This part specifies the method and basic requirements for field efficacy plot test of wheat male sterility induced by chemical male sterility (Chemical induction of male esterility). This part applies to the field efficacy plot test and efficacy evaluation of wheat male sterility induced by chemical male sterility. GB/T 17980.131-2004 Guidelines for field efficacy test of pesticides (II) Part 131: Test of wheat male sterility induced by chemical hybridization agent GB/T17980.131-2004 Standard download decompression password: www.bzxz.net
This part specifies the method and basic requirements for field efficacy plot test of wheat male sterility induced by chemical male sterility (Chemical induction of male sterility). This part applies to the field efficacy plot test and efficacy evaluation of wheat male sterility induced by chemical male sterility.

JCSs 65. 1CO
National Standard of the People's Republic of China
GB/T17980.1312004Www.bzxZ.net
Guidelines for the ficeld efficacy trials ( I )-Part 131: Chemical induclion of male sterility induce male yeld in wheat
Pesticide
Guidelines for the ficeld efficacy trials ( I )-Part 131: Chemical induclion of male sterility induce male yeld in wheat whea12004-03-03 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration
2004-08-01 Implementation
GB/T17980.131—2004
The efficacy test is the first important work of China's pesticide registration and the first is the labeling of pesticide products: the label is a guide for enterprises to use pesticides reasonably. In order to standardize the efficacy test methods of pesticides, make them more scientific and in line with international standards, and make our products more easily recognized by the world, we have formulated the efficacy test criteria of my country. This series of standards considers the field efficacy test criteria of the European and Mediterranean Plant Protection Organization (EPP) and the similar criteria of the United Nations Agriculture Organization (FAO) in the Asia-Pacific region. It is formulated based on the actual situation in China and after a large number of field tests. Chemical maleicides use chemical methods to induce male fertility in wheat, produce hybrid wheat varieties: In order to ensure the maleicide effect of chemical maleicides, control production points, and produce pure seeds, determine the dosage and period of the agent, and provide a good basis for the evaluation of the efficacy of chemical maleicides in wheat and the safe and reasonable use of the technology, this part of G/T17S was formulated. This part is one of the standards of the series of standards for efficacy test of medicinal materials (>), but it is an independent part. This part was issued by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this part is the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this part are Wei Fuqi, Yao Jinge Wenxue, Zhang Jia, Zhang Aimin, and Men Weiying. This part is edited by the Pesticide Control Institute of the Ministry of Agriculture. 54
1 Scope
Standards for field efficacy test (III)
CH/T:17 980.131—2004
Part 131: Tests on male sterility induced by chemical maleicides in wheat This part specifies the method and basic requirements for the plot test of chemical maleicides (chemical maleicides) to induce male sterility in wheat.
This part is applicable to the plot test and efficacy evaluation of chemical maleicides to induce male sterility in wheat. 2 Test Conditions
2.1 Selection of crop varieties (parents)
This method is suitable for winter wheat, winter wheat, and winter wheat varieties that are suitable for local ecological and agricultural conditions. The parent combination is properly selected and the flowering period of the parents is reasonably arranged. The male parent's opening period is not as good as the red parent, the parent has a large opening angle, large anthers, long filaments, more pollen, good pollen emission performance, regular regulation, long flowering period, long head and head length, and heading period is shorter than the male parent. The height of the interplants should be small. At the same time, the height of the interplants should be 5cm~10cm higher than that of the male parent, which is convenient for breeding. The number of interplants (seedlings) should be at least 2-4. 2.2 Cultivation conditions (soil quality, fertility, crop yield) should be consistent, and the actual situation of the site, sowing time, sowing tray, sowing method and other local agronomic measures should be taken into consideration. 3 Experimental design and arrangement 3.1 Experimental agents 3. 1. 1 Test agent
Lamin drug name, product name, product number, dosage form, content and manufacturer. The test agent has four doses of high, medium, low and medium. The purpose of setting the dose is to test the safety of the test drug for children) or according to the agreement (the test party and the test undertaker sign a test agreement: the drug package is completed,
3. 1.2 Control agent
Control agents should be designed! All products have been proven to have good effects in actual combat. They should be used in the application of the drug and sprayed at the most effective application period. Blank control (K) spray water. If the test agent is a current agent, each single wave in the standard should also be set as a control. 3.2 Plot arrangement
3. 2. 1. The experimental plan does not return the dosage. The blank control and other plots are randomly arranged. In special cases, it should be mentioned that at least 4 groups of tests should be conducted with one female parent and the corresponding male parent as a group. The parents in each group are arranged alternately and surrounded by the cross. For example: 4 varieties (series × 4 test ages × spraying periods, the completely randomized area is set as the variety area). The high area is the micro-seat for the spraying, and the spraying period is the secondary area. 3.2.2 Plot area and vertical area: 6r, the plot x should have 5 kinds of crops-6 rows. The number of times is at least 4 times. 3.3 Application method 3.3.1 Application method According to the instructions and labels, the Changzhou funding method is applied. GB/T17980.131-20D4 3.3.2 Type of spray applicator 3.3.3 Time and frequency of spraying 3.3.3.4 Type of spray applicator 3.3.4.5 Type of spray applicator 3.3.4.6 Type of spray applicator 3.3.4.7 Type of spray applicator 3.3.4.8 Type of spray applicator 3.3.4.9 Type of spray applicator 4.4.10 Type of spray applicator 4.5.2 Type of spray applicator 5.6.7 Type of spray applicator 6.8.8 Type of spray applicator 7.9.9 Type of spray applicator 8.10 Type of spray applicator 9.10 Type of spray applicator 10.10 Type of spray applicator 11.10 Type of spray applicator 12.10 Type of spray applicator 13.10 Type of spray applicator 14.10 Type of spray applicator 15.10 Type of spray applicator 16.10 Type of spray applicator 17.10 Type of spray applicator 18.10 Type of spray applicator 19.10 Type of spray applicator 20.10 Type of spray applicator 21.10 Type of spray applicator 22.10 Type of spray applicator 23.10 Type of spray applicator 24.10 Type of spray applicator 25.10 Type of spray applicator 26.10 Type of spray applicator 27.10 Type of spray applicator 28.10 Type of spray applicator 29.10 Type of spray applicator 30.10 Type of spray applicator 31.10 Type of spray applicator 32.10 Type of spray applicator 33.10 Type of spray applicator 34.10 Type of spray applicator 35 3.3.4 Use the data of dosage and water use according to the dosage and water use of the standard. The dosage of the common drugs is expressed as active ingredient/hm. The water use is expressed as T./-1. When the dosage is required, the effect of the drug can be determined according to the test method, the type of sprayer, and the water quality determined by local experience.
3.3.5 Data of drugs used for the prevention and control of pests and non-target organisms. Use other drugs, select drugs that have no effect on the timing and effect of the test drugs, and uniformly treat all plots, separate the strong drugs from the test drugs, and control the risk of other drugs to the minimum: record the accurate data of these drug tests such as name, period, dosage, etc.!
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4. 1. 1 Meteorological data
According to the meteorological data during the test period, it can be obtained from the nearest meteorological station, such as rainfall type, temperature (daily average temperature, maximum and minimum temperature, with temperature), wind speed, light and humidity, etc.: if the meteorological data of the past 10 days before the application are sufficient, the weather conditions that affect the test results during the test period should be recorded. In particular, long-term heavy rain, long-term rainfall, etc. should be recorded. 4. 1. 2 Soil snail data
Record soil moisture, organic matter content, tillage conditions. 4.2 Temporary management data
Record soil moisture, road water, fertilization and other data. 4.3 Investigation method
4.3.1 To investigate the male affinity rate, self-pollination and fruit setting rate as well as the artificial saturation inoculation and error fruiting rate of spraying treatments, select typical plants from each treatment plot, and artificially spray 23 ears in the treatment area and the opposite period area after and before flowering, and leave 19 ears for testing. At harvest, select 2 ears in each treatment area for natural pollination to determine the self-pollination and fruit setting rate. The above candidates harvested the sugar cane together, brought it indoors for testing, checked the number of single hot fruiting grids, plant height, internode length, length of smile compensation,
4.3.2 Calculation method of efficacy
External treatment energy benefit half average safety × 100
Rate (%) = (1-
Control bagged average number of grains per ear!
Treatment of convex powder light control performance variable grain whole × 1C0 self-pollination empty rate (%)
Artificial pollination fruiting rate ()
Control bagged average number of grains
Treatment of artificial pollination single half average grain tour
Control bagged sugar cane number of grains
Randomly collect 1 from the natural input and three to measure the grain weight and hair growth rate, 4.4 Investigation on the side effects of wheat growth
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During the period of seedling removal, open the ring and flower 1 large, and investigate each treatment area for phenomena that affect the growth and development of young plants, such as leaf temperature injury: heat quotient, internode length, and sticky long shoots. Samples were taken for microscopic examination to see if the morphology was normal. 5
4.5 Evaluation of male sterility effect
a) Different chemical hybrid agents or total effect on sterilization of young plants: 1) Insist on the susceptibility of varieties to the chemical series of male sterilizers: 2) The chemical scheme had male sterilization effect on young plants at different application periods; 3) Reverse technology and appropriate tailoring 4.6 The effect of cross-fertilization and other conditions on the seed killing rate of small and medium-sized plants. The low cross-fertilization rate reflects the influence of the chemical system on the whole flower device. At the same time, it determines the seed production, yield and seed production purity. The effect of the chemical maleicide on the seed production process: a) Determination of the optimal treatment period of the chemical maleicide and the evaluation of its seed production value + b) The morphological and cow changes of the chemical maleicide. 4.7 Product and quality analysis. The yield of small and medium-sized plants, the quality of the seeds and the quality of the seeds.
5 Summary and discussion
Based on the results of the experiment, comprehensive and evaluative opinions and suggestions were put forward, including the optimal concentration, spraying period, appropriate application technology of the chemical insecticide, its effect on seed production and its breeding, factors and precautions affecting seed production, applicability, etc.
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