This standard specifies the methods and basic requirements for the efficacy test of fungicides against citrus storage diseases, including Penicillium italicum, Penicillium digitatum, Diplodia natalensis, and Phomopiscitri. This standard applies to the efficacy test and efficacy evaluation of fungicides for the prevention and control of citrus storage diseases during storage in citrus warehouses. Other efficacy tests shall be carried out in accordance with this standard. GB/T 17980.39-2000 Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Controlling Citrus Storage Diseases GB/T17980.39-2000 Standard Download Decompression Password: www.bzxz.net
This standard specifies the methods and basic requirements for the efficacy test of fungicides for controlling citrus storage diseases, including Penicillium italicum, Penicillium digitatum, Diplodia natalensis, and Phomopiscitri. This standard applies to the efficacy test and efficacy evaluation of fungicides for controlling citrus storage diseases during storage in citrus warehouses. Other efficacy tests shall be carried out in accordance with this standard.

GB/T17980.39-2000
Field efficacy test is one of the important contents of pesticide registration management and an important technical basis for the formulation of pesticide product labels. Labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines in the Asia-Pacific region of the Food and Agriculture Organization of the United Nations (FAO). It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Citrus storage diseases are the main diseases of citrus during the storage period after harvest, and fungicides are often used for prevention and control. In order to determine the optimal concentration of pesticides for the prevention and control of citrus storage diseases, test the effect of pesticides on citrus storage diseases and the impact on citrus quality, and provide a basis for the efficacy evaluation and safe and rational use technology of fungicide registration, this standard is to be formulated. This standard is one of the standards in the series of standards for field efficacy tests of pesticides (-), but it is an independent standard. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This standard is drafted and interpreted by the Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this standard are Liu Naizhi, Wu Xinping, Gu Baogen, and Zhu Qinghua. 50
1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I)
Fungicides against store disease of citrus
Pesticide
Guidelines for the field efficacy trials (I)Fungicides against store disease of citrusGB/T17980.392000
This standard specifies the methods and basic requirements for efficacy trials of fungicides against store diseases of citrus, including Penicillium italicum, Penicillium digitatum, Diplodia natalensis and Phomopis citri.
This standard applies to efficacy trials and efficacy evaluation of fungicides for registration against citrus storage diseases during storage in citrus warehouses. Other efficacy trials shall be carried out in accordance with this standard.
2 Test conditions
2.1 Selection of test objects, crops and varieties The test object is citrus storage diseases.
The test crop is the citrus fruit after harvest. The citrus varieties that are susceptible to diseases during storage are selected. Record the variety name. 2.2 Environmental conditions
The test fruits must come from the same source, and the fruit size and variety characteristics must be similar. Remove the fruit with disease scars and mechanical damage, wash the test fruit and wait for processing.
During the test, the storage conditions of the test fruit must be consistent; under refrigeration, the characteristics of the variety should be considered to keep the storage conditions in the most suitable state.
The test can be carried out in different regions and different seasons (autumn, winter, summer), and multiple varieties can also be tested in different years. During the test, the fruits that are not treated should be stored separately. 3 Experimental design and arrangement
3.1 Drugs
3.1.1 Experimental drugs
Indicate the drug's trade name/code, Chinese name, generic name, dosage form, content and manufacturer. The experimental drug shall be treated with no less than three doses or the dosage specified in the agreement (the trial agreement signed by the trial entrusting party and the trial undertaking party). 3.1.2 Control drugs
Control drugs must be registered products that have been proven to have good efficacy in practice. The type and mode of action of the control drug should be similar to that of the experimental drug and the commonly used dose should be used. Special circumstances may depend on the purpose of the trial. Approved by the State Administration of Quality and Technical Supervision on February 1, 2000 and implemented on May 1, 2000
3.2 Experimental arrangements
GB/T 17980.39--2000
The experimental drugs, control drugs and blank controls are randomly arranged in the warehouse. For each treatment, 100 to 200 fruits are stored at room temperature, and 400 to 500 fruits are stored in cold storage, and this is repeated 4 to 5 times.
3.3 Application method
3.3.1 Application method
Perform according to the requirements of the agreement and the instructions on the label. Generally, single fruits and large packages are stored in isolation after dipping, fumigation and spraying. 3.3.2 Equipment used
If spraying, select the equipment commonly used in production, and record all the information on the type of equipment used and operating conditions (operating pressure, nozzle diameter). The application of pesticides should ensure that the dosage is accurate and evenly distributed. Any deviation in the dosage exceeding ±10% must be recorded. 3.3.3 Application time and number
Perform according to the requirements of the agreement and the instructions on the label, and record the date of application. 3.3.4 Dosage and capacity used
Use according to the requirements of the agreement and the dosage indicated on the label. Usually the content of active ingredients in the agent is expressed as g/t (grams per ton). At the same time, record the multiple of medication and the amount of liquid medication. When adding wax, record the type and amount of wax. 4 Investigation, recording and measurement methods
4.1 Meteorological data
During the test, record the average temperature, relative humidity and air components (carbon dioxide, ethylene, etc.). 4.2 Investigation methods, time and frequency
4.2.1 Investigation method
Investigate the number of rotten fruits and the pathogenic pathogens in each treatment. 4.2.2 Investigation time and frequency
Storage at room temperature: The first investigation is carried out 30 days after treatment, and then every half a month, for a total of three investigations. Refrigerated storage: From the start of refrigeration, investigate once a month, for a total of four investigations. 4.2.3 Calculation method of drug efficacywww.bzxz.net
The drug efficacy is calculated according to formula (1) and formula (2):
Number of rotten fruits
Rotten fruit rate (%)-
Total number of fruits investigated
Storage disease prevention effect (%)=
Where: CK.-Raw fruit rate of blank control treatment; PT-Raw fruit rate of test agent treatment.
4.3 Direct effect on fruit
X 100-
·(1)
Observe whether the drug causes phytotoxicity to the fruit. If spots appear on the fruit, they must be recorded. Any changes in color and taste must be accurately described. 4.4 Effect on other pests and diseases
Any effect on other pests and diseases should be recorded, including beneficial and unbeneficial effects. 4.5 Product yield and quality
Factors that affect the taste and aroma of the fruit must be recorded. 5 Results
Use Duncan's new multiple range (DMRT) method to statistically analyze the test data. Write a formal test report and analyze and evaluate the test results. The test report should list the original data. 0G
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