NY/T 1113-2006 Microbial Fertilizer Terminology NY/T1113-2006 Standard download decompression password: www.bzxz.net
This standard specifies the main terms in terms of microbial fertilizer product types, strains, culture media, sterilization, production and quality inspection. This standard is applicable to the fields of microbial fertilizer production, quality inspection, application, scientific research and teaching.

ICS65.080
Agricultural Industry Standard of the People's Republic of China
NY/T1113—2006
Terms of microbial fertilizer
Terms of microbial fertilizer2006-07-10 Issued
Ministry of Agriculture of the People's Republic of China
Implementation on 2006-10-01
Appendix A and Appendix B of this standard are informative appendices. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this standard is the Microbial Fertilizer Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture. The main drafters of this standard are Yang Xiaohong, Li Jun, Shen Delong, Jiang Xin, Ge Yifan, Cao Fengming, Li Li, and Chen Huijun. httn://wwwfoodmateNY/T1113—2006
1 Scope
Terms of microbial fertilizer
NY/T1113—2006
This standard specifies the main terms of microbial fertilizer product types, strains, culture media, sterilization, production and quality inspection. This standard is applicable to the fields of microbial fertilizer production, quality inspection, application, scientific research and teaching. 2 Product types
Microbial fertilizer: Biofertilizer is a product containing specific living microorganisms, which is used in agricultural production. Through the life activities of the microorganisms contained therein, it increases the supply of plant nutrients or promotes plant growth, improves yield, and improves the quality of agricultural products and the agricultural ecological environment. Note: Currently, microbial fertilizers include microbial inoculants (2.2), compound microbial fertilizers (2.3) and bio-organic fertilizers (2.4) 2.2
Microbial inoculant
microbialinoculant
[Microbial] Inoculant
A live bacterial product made by directly using one or more target microorganisms after industrial production and proliferation, or by concentrating (6.10) or adsorbing (6.11) on a carrier (6.9).
Single species inoculant A microbial inoculant made from one microbial species (2.2). 2.2.2
Compound inoculant
multiple species inoculant
A microbial inoculant made from two or more microbial species that are not mutually antagonistic (2.2). 2.2.3
bacterial inoculant
microbial inoculant made from bacteria as the production strain (2.2). 2.2.4
actinomycetic inoculant
microbial inoculant made from actinomycetes as the production strain (2.2). 2.2.5
fungalinoculant
microbial inoculant made from fungi as the production strain (2.2). 2.2.6
azotobacteriainoculant microbial inoculant made from self-generated nitrogen-fixing bacteria and/or combined nitrogen-fixing bacteria as the production strain (2.2). 2.2.7
rhizobia inoculant
rhizobia inoculant
microbial inoculant (2.2) made from rhizobia (3.12.2) as the production strain. 2.2.8
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silicatebacteriainoculantmicrobial inoculant (2.2) made from silicate bacteria (3.12.3) as the production strain. 2.2.9
inoculantofphosphate-solubilizingmicroorganismmicrobial inoculant (2.2) made from phosphate-solubilizingmicroorganism (3.12.4) as the production strain. 2.2.10
inoculantofphotosyntheticbacteriamicrobial inoculant (2.2) made from photosyntheticbacteria (3.12.5) as the production strain. 2.2.11
Mycorrhizal fungi inoculant A microbial inoculant (2.2) made from mycorrhizal fungi (3.12.6) as the production strain. 2.2.12
Inoculant of plant growth-promoting rhizosphere microorganism A microbial inoculant (2.2) made from plant growth-promoting rhizosphere microorganism (3.12.8) as the production strain. 2.2.13
Organic matter-decomposing inoculant A microbial inoculant (2.2) that can accelerate the decomposition and maturation of various organic materials (including crop straw, livestock and poultry manure, domestic garbage and urban sludge, etc.).
Note: Rewrite NY6092002. Terms and Definitions 3. 2.2.14
Bioremediating inoculant A microbial inoculant that can reduce the concentration of harmful substances in the environment, reduce toxicity or render them harmless through the growth and metabolism of microorganisms (2.2).
Compound microbial fertilizer Compound microbial fertilizer Purpose A live bacterial product made by combining microorganisms with nutrients after industrial production and proliferation. Note: Rewrite NY/T798—2004, Terms and Definitions 32.4
Biological organic fertilizer Microbial organic fertilizer Purpose A live bacterial product made by combining microorganisms with organic materials mainly derived from animal and plant residues (such as livestock and poultry manure, crop stalks, etc.) and harmlessly treated after industrial production and proliferation. Note: Rewrite NY884-2004.Terms and Definitions 33 Species
Species
In microbiology, it is composed of strains with extremely similar phenotypic characteristics and stable genetic traits, and has obvious differences from strains of other groups.
Strain
Descendants of single cells or pure cultures belonging to the same species but from different sources. 3.3
Colony
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A visible cell aggregate with certain morphological characteristics formed by the growth and reproduction of microorganisms on a solid matrix. Lawn
A culture formed by a large number of microbial cells densely growing on the surface of a solid culture medium and connected to each other (6.5). Isolation
The technology of separating individual microorganisms from a sample containing microorganisms. Purification
The technology of isolating individual microorganisms of the same species from a mixed microbial population. Screening
The process of selecting the target strain from a population of microorganisms by using relevant techniques. Identification
The process of observing and measuring the characteristics of unknown microbial strains and determining the taxonomic status of the microorganism by comparative analysis based on standardized parameters or retrieval systems.
Degeneration
The phenomenon that the specific characteristics of the strain decrease or disappear from generation to generation. 3.10
Rejuvenation
The process of restoring the original characteristics of the strain in response to degeneration (3.9). 3.11
Preservation of microorganism The microbiological technology that enables the strain to maintain its vitality, inherent genetic and physiological and biochemical characteristics, and morphological characteristics. 3.12
Production strains
AzotobacterianitrogenfixingbacteriaA general term for various bacteria that have the function of biological nitrogen fixation3.12.2
Rhizobia
A type of Gram-negative rod that can coexist with leguminous plants, form root nodules, and carry out biological ammonia fixation. 3.12.3
Silicate bacteriasilicatedissolvingbacteriaBacteria that can decompose silicate minerals and release potassium nutrients. Note: At present, the main strains used for production are Bacillus mucilagirioszus and Bacillus adaphicus. 312.4
Phosphate solubilizingmicroorganismA general term for microorganisms that can decompose organic phosphorus compounds or dissolve inorganic phosphorus compounds. 3
County Partner District
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Photosynthetic bacteriaphotosyntheticbacteriaBacteria that can use light energy for cell metabolic activities. 3.12.6
Mycorrhizal fungi
Fungi that can form symbiotic mycorrhizae with plant roots 3.12.7
Arbuscular mycorrhizal fungiFungi that can form arbuscular mycorrhizae with plant roots, referred to as AM fungi. 3.12.8
Plant growth-promoting rhizosphere microorganismsA general term for microorganisms that exist in the plant rhizosphere and can produce plant growth substances or promote plant growth by inhibiting harmful microorganisms. Including plant growth-promoting rhizosphere bacteria (PGPR) and plant growth-promoting rhizosphere fungus (PGPF). 4 Culture medium
Culture medium medium; culture medium
Artificially prepared nutrient matrix suitable for the growth, metabolism, reproduction and preservation of microorganisms. 4.2
seedmedium
seed medium
medium (4.1) prepared to obtain microbial inoculum (6.2). 4.3
fermentation medium
fermentationmedium
medium (4.1) prepared to obtain the final product (bacteria and metabolites) of microbial fermentation. 4.4
natural medium
natural medium
medium (4.1) made from animal and plant tissues or microbial cells and their extracts and crude digestion products, which is nutrient-rich but the exact composition is unknown.
synthetic medium definedmedium
medium (4.1) prepared from chemical reagents with known composition and content. 4.6
semi-synthetic medium
semi-defined medium
medium (4.1) containing both natural ingredients and chemical reagents. 4.7
selected medium
Selective medium
A medium designed according to the special nutritional requirements of a certain microorganism or its characteristics to certain chemical or physical factors (4.1), whose function is to make a certain bacteria in a mixed flora become the dominant flora. 4.8
differential medium
Differential medium
A medium with inhibitors or indicators added to distinguish different types of microorganisms (4.1). 4
5 Sterilization
Sterilization
Measures to kill or eliminate all microorganisms by physical or chemical methods. 5.2
High-pressure steam sterilization Method of sterilization using high-pressure steam. 5.3
Intermittent sterilization
Fractional sterilization
Refers to a method of sterilizing repeatedly with normal pressure steam at intervals for a certain period of time. 5.4
dry heat sterilization
dry heat sterilization
sterilization method using heated high temperature air. 5.5
flame sterilization
sterilization method using flame high temperature burning. 5.6
ionizing radiation sterilization method using radiation produced by radioisotopes (such as Co or 137Cs) for sterilization. 5.7
microwave sterilization
sterilization method using electromagnetic waves.
ultrawiolet light sterilization
sterilization method using ultraviolet light irradiation. 5.9
filtration sterilization
filtration sterilization method using mechanical retention technology (such as filtration, adsorption) to remove microorganisms from the medium. 5.10
chemical sterilization
chemical sterilization
method of sterilization using chemical agents. 6
inoculation
the process of transferring the target microorganism to the culture medium according to the requirements of aseptic operation technology. 6.2
inoculum
inoculum
the microbial culture used to start a new culture in the industrial production of microorganisms (6.5) 6.3
NY/T11132006
NY/T1113—2006
inoulumdose
the ratio of the amount (volume or mass) of the inoculum (6.2) to the amount (volume or mass) of the inoculum. 6.4
cultivation
Methods and techniques for growing and reproducing target microorganisms and producing metabolites under suitable conditions. 6.5
culture
The growth of a specific type of microorganism formed in the culture medium (4.1) after inoculation (6.1) and cultivation (6.4). 6.6
pure culture
The process of cultivating only one type of microorganism (6.4) to grow and reproduce. 6.7
inoculumenlargementThe technology and process of gradually expanding the production strain through a series of steps to obtain a certain quantity and quality of culture (6.5). 6.8
fermentation
The process of cultivating microorganisms in an industrial production method to obtain the final product (microorganism and metabolites). 6.9
Carrier
Solid material used to adsorb target microorganisms, suitable for their survival, and safe for humans, animals, plants and the environment. 6.10
condensation
The process of reducing the water content of fermentation broth by a certain technology or method to increase the number of target microorganisms and the content of metabolites. 6.11
adsorption
The process of mixing fermentation broth with carrier (6.9) to make the target microorganisms adhere to the carrier. 6.12
granulation
The process of making microbial fertilizer (2.1) into granular dosage form. 7 Quality inspection
appearance
The external appearance of the sample.
moisturepercentage
The mass lost by the sample when baked at 105℃ for 4h~6h, expressed as mass percentage. 7.3
Particle size
The mass percentage of the sample passing through the specified standard test sieve. 7.4
Content of organic matter
The amount of organic matter in the sample determined by the volumetric method, expressed in mass percentage. Note: The volumetric method can refer to NY525-2002 (Organic Fertilizer). Total primary nutrient
The sum of total nitrogen, available phosphorus pentoxide and potassium oxide, expressed in mass percentage. [GB15063-2001,Definition 3.8]
Effective bacteria functionalmicroorganism; effectivemicroorganism The target microbial population in the sample.
Effective [live] bacteria numberof functionalmicroorganism The number of effective bacteria (7.6) per gram or per milliliter of sample. 7.8
Contaminating microorganism
Other bacteria in the sample other than effective bacteria (7.6). 7.9
Number of contaminating microorganism Number of contaminating microorganism The number of contaminating microorganism (7.8) per gram or per milliliter of sample. 7.10
Percentage of contaminating microorganism The percentage of the number of contaminating microorganism (7.9) in the sample to the sum of the number of effective bacteria (7.7) and the number of contaminating microorganism (7.9). 7.11
fecal coliforms
NY/T11132006
A general term for Gram-negative, non-spore-forming bacilli that can ferment lactose, produce acid and gas, and are aerobic or facultatively anaerobic at 44.5℃±0.5℃.
[GB/T19524.1—2004, definition 2]
fecal coliforms numberoffecal coliforms The maximum probable number (MPN) of fecal coliforms (7.11) per gram or milliliter of sample. [GB/T19524.1—2004, definition 2]
mortality of ascarid eggThe percentage of dead ascarid eggs in the sample to the total number of ascarid eggs7.14
contentof heavy metalThe total amount of arsenic (As), lead (Pb), cadmium (Cd), chromium (Cr), and mercury (Hg) compounds contained in the sample. 7.15
Shelf-life
The period during which the quality of microbial fertilizer is maintained under the storage conditions indicated on the label. 7.16
microbial fertilizer effect
microbial fertilizer effect on crop yield, quality, disease (pest) resistance and stress resistance, as well as soil fertility. 7
NY/T1113—2006
semi-synthetic culture medium
shelf life
purification…
pure culture
arbuscular mycorrhizal fungi
growth-promoting agents
single agents
ionizing radiation sterilization
fermentation·
fermentation medium·
actinomycete agents
fecal coliform
fecal coliform count
compound agents
compound microbial fertilizer
dry heat sterilization
high-pressure steam sterilization
rhizobia
rhizobia agents|| tt||Nitrogen-fixing bacteria
Nitrogen-fixing bacteria agent
Photosynthetic bacteria
Photosynthetic bacteria agent
Silicate bacteria
Appendix A
(Informative Appendix)
Chinese Index
Silicate bacteria agent·
Filter sterilization method
Water content?
Synthetic culture medium
.··2.2.7
Product China
Chemical sterilization method·
Ascaris egg mortality rate
Flame sterilization method
Identification culture medium
Intermittent sterilization method
Inoculation·
Inoculation volume| |tt||Inoculum-
Mycorrhizal agent
Mycorrhizal fungi
[Microbial] agent
Colony·
Mushroom·
Strain preservation
Strain·
Sterilization·
Culture medium
Culture
Phosphorus-dissolving microorganisms
Phosphorus-dissolving microbial agent
Bioremediation agent
Bio-organic fertilizer
Natural culture medium
Microwave sterilization method
Microbial fertilizer…
Microbial fertilizer effect
Microbial inoculant·
Bacterial agent
Selective culture medium
Organic material decomposition agent
Organic matter content·
Effective [live] bacteria count
Effective bacteria·
Miscellaneous bacteria·
Miscellaneous bacteria rate
Miscellaneous bacteria count·
Carrier·
Fungal agent-
Plant growth promoting rhizosphere microorganisms
Heavy metal content
Seed expansion culture
Seed culture medium
Ultraviolet sterilization method
Total nutrients
NY/T1113—2006
NY/T1113—2006
actinomycetic inoculant | ilization
colonyt
compound microbial fertilizercondensation :
contaminatingmicroorganism
content of organic matter
content of heavy metal
cultivation
culture
culture collection
culture medium
defined medium
degeneration
differential medium
d ry heat sterilization
Appendix B
(Informative appendix
English index
effective microorganis
fecal coliforns
fermentation
fermentation medium
filtration sterilization
flame sterilization
fractional sterilization .
fungal inocul ant
granulation
high-pressure steam sterilization1
identification
inoculant of phosphate-solubilizingmicroorganisn
inoculant of photosynthetic bacteria7.6
.....2.2.10
inoculant of plant growth-promoting rhizospheremicroorganistr
inoculation
inoculum
inoculum dose
inoculum enlargement
ionizing radiation sterilizationisolation8]
Effective bacteria functionalmicroorganism; effectivemicroorganismThe target microbial population in the sample.
Effective [live] bacteria numberof functionalmicroorganismThe number of effective bacteria (7.6) per gram or per milliliter of sample. 7.8
Contaminating microorganism
Other bacteria in the sample other than effective bacteria (7.6). 7.9
Number of contaminating microorganismNumber of contaminating microorganismThe number of contaminating microorganism (7.8) per gram or per milliliter of sample. 7.10
Percentage of contaminating microorganismThe percentage of the number of contaminating microorganism (7.9) in the sample to the sum of the number of effective bacteria (7.7) and the number of contaminating microorganism (7.9). 7.11
fecal coliforms
NY/T11132006
A general term for Gram-negative, non-spore-forming bacilli that can ferment lactose, produce acid and gas, and are aerobic or facultatively anaerobic at 44.5℃±0.5℃.
[GB/T19524.1—2004, definition 2]
fecal coliforms numberoffecal coliforms The maximum probable number (MPN) of fecal coliforms (7.11) per gram or milliliter of sample. [GB/T19524.1—2004, definition 2]
mortality of ascarid eggThe percentage of dead ascarid eggs in the sample to the total number of ascarid eggs7.14
contentof heavy metalThe total amount of arsenic (As), lead (Pb), cadmium (Cd), chromium (Cr), and mercury (Hg) compounds contained in the sample. 7.15
Shelf-life
The period during which the quality of microbial fertilizer is maintained under the storage conditions indicated on the label. 7.16
microbial fertilizer effect
microbial fertilizer effect on crop yield, quality, disease (pest) resistance and stress resistance, as well as soil fertility. 7
NY/T1113—2006
semi-synthetic culture medium
shelf life
purification…
pure culture
arbuscular mycorrhizal fungi
growth-promoting agents
single agents
ionizing radiation sterilization
fermentation·
fermentation medium·
actinomycete agents
fecal coliform
fecal coliform count
compound agents
compound microbial fertilizer
dry heat sterilization
high-pressure steam sterilization
rhizobia
rhizobia agents|| tt||Nitrogen-fixing bacteria
Nitrogen-fixing bacteria agent
Photosynthetic bacteria
Photosynthetic bacteria agent
Silicate bacteria
Appendix A
(Informative Appendix)
Chinese Index
Silicate bacteria agent·
Filter sterilization methodwww.bzxz.net
Water content?
Synthetic culture medium
.··2.2.7
Product China
Chemical sterilization method·
Ascaris egg mortality rate
Flame sterilization method
Identification culture medium
Intermittent sterilization method
Inoculation·
Inoculation volume| |tt||Inoculum-
Mycorrhizal agent
Mycorrhizal fungi
[Microbial] agent
Colony·
Mushroom·
Strain preservation
Strain·
Sterilization·
Culture medium
Culture
Phosphorus-dissolving microorganisms
Phosphorus-dissolving microbial agent
Bioremediation agent
Bio-organic fertilizer
Natural culture medium
Microwave sterilization method
Microbial fertilizer…
Microbial fertilizer effect
Microbial inoculant·
Bacterial agent
Selective culture medium
Organic material decomposition agent
Organic matter content·
Effective [live] bacteria count
Effective bacteria·
Miscellaneous bacteria·
Miscellaneous bacteria rate
Miscellaneous bacteria count·
Carrier·
Fungal agent-
Plant growth promoting rhizosphere microorganisms
Heavy metal content
Seed expansion culture
Seed culture medium
Ultraviolet sterilization method
Total nutrients
NY/T1113—2006
NY/T1113—2006
actinomycetic inoculant | ilization
colonyt
compound microbial fertilizercondensation :
contaminatingmicroorganism
content of organic matter
content of heavy metal
cultivation
culture
culture collection
culture medium
defined medium
degeneration
differential medium
d ry heat sterilization
Appendix B
(Informative appendix
English index
effective microorganis
fecal coliforns
fermentation
fermentation medium
filtration sterilization
flame sterilization
fractional sterilization .
fungal inocul ant
granulation
high-pressure steam sterilization1
identification
inoculant of phosphate-solubilizingmicroorganisn
inoculant of photosynthetic bacteria7.6
.....2.2.10
inoculant of plant growth-promoting rhizospheremicroorganistr
inoculation
inoculum
inoculum dose
inoculum enlargement
ionizing radiation sterilizationisolation8]
Effective bacteria functionalmicroorganism; effectivemicroorganismThe target microbial population in the sample.
Effective [live] bacteria numberof functionalmicroorganismThe number of effective bacteria (7.6) per gram or per milliliter of sample. 7.8
Contaminating microorganism
Other bacteria in the sample other than effective bacteria (7.6). 7.9
Number of contaminating microorganismNumber of contaminating microorganismThe number of contaminating microorganism (7.8) per gram or per milliliter of sample. 7.10
Percentage of contaminating microorganismThe percentage of the number of contaminating microorganism (7.9) in the sample to the sum of the number of effective bacteria (7.7) and the number of contaminating microorganism (7.9). 7.11
fecal coliforms
NY/T11132006
A general term for Gram-negative, non-spore-forming bacilli that can ferment lactose, produce acid and gas, and are aerobic or facultatively anaerobic at 44.5℃±0.5℃.
[GB/T19524.1—2004, definition 2]
fecal coliforms numberoffecal coliforms The maximum probable number (MPN) of fecal coliforms (7.11) per gram or milliliter of sample. [GB/T19524.1—2004, definition 2]
mortality of ascarid eggThe percentage of dead ascarid eggs in the sample to the total number of ascarid eggs7.14
contentof heavy metalThe total amount of arsenic (As), lead (Pb), cadmium (Cd), chromium (Cr), and mercury (Hg) compounds contained in the sample. 7.15
Shelf-life
The period during which the quality of microbial fertilizer is maintained under the storage conditions indicated on the label. 7.16
microbial fertilizer effect
microbial fertilizer effect on crop yield, quality, disease (pest) resistance and stress resistance, as well as soil fertility. 7
NY/T1113—2006
semi-synthetic culture medium
shelf life
purification…
pure culture
arbuscular mycorrhizal fungi
growth-promoting agents
single agents
ionizing radiation sterilization
fermentation·
fermentation medium·
actinomycete agents
fecal coliform
fecal coliform count
compound agents
compound microbial fertilizer
dry heat sterilization
high-pressure steam sterilization
rhizobia
rhizobia agents|| tt||Nitrogen-fixing bacteria
Nitrogen-fixing bacteria agent
Photosynthetic bacteria
Photosynthetic bacteria agent
Silicate bacteria
Appendix A
(Informative Appendix)
Chinese Index
Silicate bacteria agent·
Filter sterilization method
Water content?
Synthetic culture medium
.··2.2.7
Product China
Chemical sterilization method·
Ascaris egg mortality rate
Flame sterilization method
Identification culture medium
Intermittent sterilization method
Inoculation·
Inoculation volume| |tt||Inoculum-
Mycorrhizal agent
Mycorrhizal fungi
[Microbial] agent
Colony·
Mushroom·
Strain preservation
Strain·
Sterilization·
Culture medium
Culture
Phosphorus-dissolving microorganisms
Phosphorus-dissolving microbial agent
Bioremediation agent
Bio-organic fertilizer
Natural culture medium
Microwave sterilization method
Microbial fertilizer…
Microbial fertilizer effect
Microbial inoculant·
Bacterial agent
Selective culture medium
Organic material decomposition agent
Organic matter content·
Effective [live] bacteria count
Effective bacteria·
Miscellaneous bacteria·
Miscellaneous bacteria rate
Miscellaneous bacteria count·
Carrier·
Fungal agent-
Plant growth promoting rhizosphere microorganisms
Heavy metal content
Seed expansion culture
Seed culture medium
Ultraviolet sterilization method
Total nutrients
NY/T1113—2006
NY/T1113—2006
actinomycetic inoculant | ilization
colonyt
compound microbial fertilizercondensation :
contaminatingmicroorganism
content of organic matter
content of heavy metal
cultivation
culture
culture collection
culture medium
defined medium
degeneration
differential medium
d ry heat sterilization
Appendix B
(Informative appendix
English index
effective microorganis
fecal coliforns
fermentation
fermentation medium
filtration sterilization
flame sterilization
fractional sterilization .
fungal inocul ant
granulation
high-pressure steam sterilization1
identification
inoculant of phosphate-solubilizingmicroorganisn
inoculant of photosynthetic bacteria7.6
.....2.2.10
inoculant of plant growth-promoting rhizospheremicroorganistr
inoculation
inoculum
inoculum dose
inoculum enlargement
ionizing radiation sterilizationisolation10
inoculant of plant growth-promoting rhizospheremicroorganistr
inoculation
inoculum
inoculum dose
inoculum enlargement
ionizing radiation sterilizationisolation
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