Guidelines on efficacy evaluation of pesticedes Part 20: Herbicide control weed in tomato field
Some standard content:
ICS 65.100
Agricultural Industry Standard of the People's Republic of China
NY/T 1464.20—2007
Guidelines on efficacy evaluation of pesticides Part 20:Herbicide control weed in tomato field field2007-12-18 issued
2008-03-01 implemented
The Ministry of Agriculture of the People's Republic of China issued
NY/T1464 "Guidelines for Field Efficacy Tests of Pesticides" is a series of standards, totaling 2G parts: Part 1: Tea insecticides for control of migratory locusts;
Part 2: Insecticides for control of rice water weevils; -Part 3: Insecticides for control of cotton blind bugs; Part 4: Insecticides for control of yellow aphids; Part 5: Insecticides for control of fruit aphids; Part 6: Insecticides for control of vegetable horses; Part 7: Fungicides for control of carbon monoxide disease; Part 8: Fungicides for control of tomato virus diseases: Part! Some fungicides for controlling pepper virus diseases: Part 10: Fungicides for controlling mushroom bubble disease: Part 11: Fungicides for controlling banana black spot; Part 12: Fungicides for controlling grape powdery mildew: Part 13: Fungicides for controlling grape anthracnose: Part 14: Fungicides for controlling rice residue blight! Part 15: Fungicides for the control of wheat red blight; Part 16: Fungicides for the control of wheat root rot; Part 17: Herbicides for the control of weeds in green field; Part 18: Herbicides for the control of weeds in sesame field; Part 19: Herbicides for the control of weeds in wolfberry field; Part 20: Herbicides for the control of weeds in tomato field; Part 21: Herbicides for the control of weeds in cucumber field; Part 22: Herbicides for the control of weeds in garlic field; Part 23: Herbicides for the control of weeds in sorghum field; Part 21: Herbicides for the control of weeds in red bean field; Part 22: Herbicides for the control of weeds in tobacco seedling bed; Part 26: Test for cotton wilt accelerators. This part is Part 20 of the guidelines for inter-organic efficacy tests of pesticides. This part was proposed and managed by the Crop Management Department of the Ministry of Agriculture of the People's Republic of China. The unit of this part is the Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this part: Zhang Hongjun, Wu Zhifeng, Yang Jun, Liu Xue, Liu Yixue, Zhang Jia, Lu Jianfei, NY/T1464.20-2007
1 Scope
Guidelines for field efficacy tests of pesticides
Part 20: Herbicide control of tomato weeds This part specifies the methods and basic requirements for the field efficacy test of herbicides for controlling tomato weeds. NY/T 1464.20—2007
This part is applicable to the field efficacy test and efficacy evaluation of herbicides for controlling weeds in open field and protected tomato fields. Other field efficacy tests shall refer to this part. 2 Test conditions
2.1 Crops
Conventional varieties that are representative of the local area.
2.2 Selection of test weeds
The test site must have a variety of representative weeds, and the number distribution must be uniform. The weed composition must be consistent with the herbicide spectrum to be tested (grass, sedge, broadleaf grass, annual (annual) 2.3 Cultivation conditions
The cultivation conditions of the test plot (soil type, organic matter content, pH, cropping conditions, etc.) must be uniform and in line with local agricultural regulations.
Record the previous crops and the previously used pesticides. Avoid selecting plots where pesticides that affect the growth of the plant or the effect of the test pesticide have been used.
3 Experimental design and arrangement
3.1 Pesticides
3.1.1 Test pesticides
Indicate the code of the test pesticide, the Chinese and English common names, dosage form, dosage and manufacturer. The test agent treatment is set at high, medium, low and multiple of the medium dose (the multiple of the medium dose is set to evaluate the safety of the agent on wolfberry), or according to the agreement (the test agreement signed by the test entrusting party and the test undertaking party, the same as below). 3.1.2 Control agent
The control agent must be a registered product that has been proven to have good safety and efficacy in practice: the type and mode of action of the control agent are the same or similar to the test agent and the local commonly used dose and treatment method are used. Special circumstances can be determined according to the test age. Set manual weeding and empty house control treatment.|| tt||3.2 Plot arrangement
3.2.1 Plot arrangement
The plots for different treatments in the experiment are arranged in random blocks. In special cases, such as the experiment of controlling perennial weeds, in order to avoid the uneven distribution of perennial weeds, the plots can be arranged irregularly according to the actual situation, and it should be explained. 3.2.2 Plot area and repetition
Plot area: 20㎡～30m㎡2, each plot should include at least 2 rows of tomatoes, and the total number of seedlings should not be less than 20. Number of repetitions: at least 4 times.
3.3 Application method
NY/T 1464.20-2007
3.3.1 Instructions
Follow the protocol and label instructions, usually spraying. The method of application should be in accordance with local good agricultural practices. 3.3.2 Application equipment
Select a sprayer with stable pressure and fan-shaped nozzle for application. Ensure that the pesticide is evenly distributed on the designated spraying site of the test. Record all information such as the type of equipment used and operating conditions (operating pressure, nozzle type and height, nozzle diameter, soil mixing depth). The amount of pesticide should be accurate when applying the pesticide. If the deviation of the amount exceeds ±10%, it should be checked. Record the factors that affect the efficacy, duration and selectivity of the drug. 3.3.3 Application time and frequency
Follow the protocol requirements and label instructions. If the label or protocol does not specify the number of applications, the test should be conducted according to the test results and the action characteristics of the test agent. The application time is related to the planting period of weeds and tomatoes. a) Before transplanting tomato (mixed with soil or not mixed with soil): h) After transplanting tomato (mixed with soil or not mixed with soil): c) After transplanting tomato (full or directional spraying) Record the month and time of each application and the growth status of weeds and tomatoes at the time of application (seedlings, growth period). The same drug can be applied once or in multiple times, and the number and time of applications should be recorded. 3.3.4 Application amount and water consumption
Apply the drug according to the protocol requirements and the dosage and water consumption indicated on the label. The dosage of the drug is expressed in the name of the active ingredient/hm, and the water consumption is expressed in/hIn. When the amount of water is not specified in the agreement, the amount of water can be determined based on the mode of action of the test agent, the type of sprayer, and local experience:
3.3.5 Agents for controlling pests and diseases and non-target weeds If other agents are used: Agents that have no effect on the test agent, the control object and tomatoes should be selected, and all plots should be treated uniformly and used separately from the test agent and control agent to minimize the interference of these agents. 4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the experiment, data such as rainfall (precipitation in mm), temperature (daily average temperature, maximum and minimum humidity in mm), relative humidity (in mm), wind (in m2/s), and sunshine hours (in h/l) should be obtained from the test site or the nearest meteorological station. Especially the meteorological data on the day of application and 10 days before and after: record the bad weather during the whole test period, such as severe or continuous dry weather, rainstorm, late frost, ice pods, etc. 4.1.2 Soil data
Record the soil type, organic matter content, soil fertility, PH, soil moisture (dryness, water accumulation), etc., and also record the previous crop, yield level, etc.
4.2 Field management
If there are agricultural activities such as fertilization, irrigation, pest control, etc., the time, frequency and method should be recorded. Record the weeding time and number of weeds in the weeding plot. 4.3 Survey methods, time and frequency
4.3.1 Weed survey
Record the weed population in the plot, such as weed species, number of weeds, coverage or weight, etc. Use the absolute value method or estimated value method, and record the Chinese name and Latin name of each weed. Describe in detail the obvious symptoms of the effect of the pesticide on the weeds (such as growth inhibition, chlorosis, deformity, etc.) to accurately explain the action mode of the pesticide
4.3.1.1 Absolute value (numerical measurement survey method
NY/T 1464.20 - 2007
Calculate the number or weight of each weed, survey each plot or randomly select 3 to 4 points in each plot, and conduct sampling surveys at each point with a square area of 0.25m~1.00m2. If necessary, survey some other contents, such as the number of weeds. 4.3.1.2 Estimated value (visual inspection) survey method
Each pesticide treatment area is compared with the adjacent blank control area to estimate the relative weed population. This survey method includes the overall weed community and individual weeds, and can use indicators such as weed quantity, coverage, height and growth (such as the actual weed quantity). The estimated result can be expressed in a simple percentage (0 means no weeds, 100% means the same weeds as the blank control area), also known as the equivalent percentage effect of weed control (0 means no control effect, 100% means all weeds are controlled). The types and number of weeds in the blank control area should also be recorded. In order to overcome the difficulties of accurately estimating percentages and using homogeneous variance, the following grading standard can be used for investigation: [Grade 1: None; Grade 2: Equivalent to 0~-2.5% of the blank control area; Grade 3: Equivalent to 2.6% of the blank control area; Grade 4: Equivalent to 5.1%~0% of the blank control area; Grade 5: Equivalent to 10.1%-15% of the blank control area; Grade 6: Equivalent to 15.1%~25% of the blank control area; Grade 7: Equivalent to 25.1%~35% of the blank control area; Grade 8: Equivalent to 35.1%~67.5% of the blank control area; Grade 9: Equivalent to 67.6%~100% of the blank control area. Investigators must be trained before using this grading standard: This grading range can be used directly without converting it into an estimated value or the average of the scores.
4.3.2 Investigation time and frequency
a) Soil treatment
The first investigation: conducted after weeds emerge in the control area (generally about 15 days after application, visual inspection or adjustment of the number of plants for control efficacy); the second investigation: conducted during the vigorous weed growth period in the control area (generally about 30 days after application, to investigate the number of plants for control efficacy); the third investigation: conducted after new weeds emerge in the treatment area (---generally 45-60 days after application to investigate the number of plants for control efficacy and fresh weight for control efficacy).
h) Stem and leaf treatment
First survey: trace before application, investigate the weed species and growth period, and the percentage of major weeds (base survey); second survey: about 15 days after application (plant number control effect, quick effect survey); third survey: about 30 days after application (plant number control effect); fourth survey: 45 days to 60 days after application (plant number control effect, fresh weight control effect, lasting effect survey). 4.3.3 Efficacy calculation method
Root survey number, pre-sowing or post-sowing pre-seedling application and post-seedling application, the control effect is calculated according to formula (1). For post-seedling application except the last survey, the control effect is calculated according to formula (2), the unit is fraction (%). The calculation result is rounded to two decimal places. E-XX×100
E=Xx100
Wherein:
EPrevention effect:
X. ——Number of live weeds (or fresh weight) in the blank control area: X:—Number of remaining weeds (or fresh weight) in the treatment area; X,“—Number of weeds in the treatment area before drug application; X—Number of remaining weed nodes in the treatment area after drug application
....(13
.. (2)
NY/T 1464.20—2007
4.4 Crop Survey
4.4.1 Survey Methods
Observe the effects of pesticides on tomato growth and record the types and extent of the pesticide damage. The following requirements can be recorded: a) Record the time and symptoms of the pesticide damage in detail (such as growth inhibition, chlorosis, dead spots, deformities, etc.). b) Observe the interaction between pesticide damage and adverse factors (cultivation methods, lodging, pests and diseases, long-term high temperature or freezing damage, etc.).
For example If the fruit damage can be counted or measured, it is expressed in absolute values, such as plant height, stem thickness, degree of expansion, number of branches, number of fruits and number of deformed fruits. The number of plants measured should be no less than 15 per plot. In other cases, the degree and frequency of drug damage can be estimated by the following two methods: 1) According to the method of drug damage classification, the drug damage of each plot is graded and scored. 2) Compare the drug treatment area with the blank control area to evaluate the percentage of drug damage: Level 1: The fruit grows normally without any symptoms of damage; Level 2: The fruit is not damaged; Slight damage, less than 10% of the damage is: Level 3: Moderate damage to tomatoes, can be recovered later - no impact on yield; Level 5: Severe damage to tomatoes, difficult to recover, resulting in low yield; Level 5: Severe damage to tomatoes, cannot be recovered - resulting in obvious low yield or no yield. 4.4.2 Number of surveys
Observe 5, 1, and 5 days after application to see if there is any damage. If there is any damage, the symptoms and degree of damage should be recorded in detail, and the time required for tomatoes to resume normal growth should be observed and recorded, and the yield should be measured. 4.5 Side effect observation ||tt ||Record the effects of the test agent on non-target organisms. If other agents are used to control pests and non-target weeds, the accurate data of the application of such agents should be recorded (such as agent name, application time, application dosage, etc.): 4.6 Tomato yield
Tomatoes are a vegetable variety harvested in batches, and the difference between individual tomato plants is relatively large, so each time the fruit yield is measured, all tomato plants in the plot should be included. Generally, they are harvested every 5 to 7 days until the harvest is completed. The total yield of the plot is the sum of the yields of each batch, usually in kg/ hm2 indicates.
If required, the quality of the pesticide should be graded according to the relevant standards and relevant regulations. 5 Statistical analysis
The obtained data were statistically analyzed by using the biostatistical method (DMRT method). 6 Results and report writing
Analyze and evaluate the results and issue a formal test report. If the agent shows signs of long-lasting effect in the test, a post-crop safety test should be carried out.
NY/T 1464.20-2007
Agricultural Industry Standard of the People's Republic of China
Guidelines for Field Efficacy Tests of Pesticides
Part 2: Herbicides for Control of Weeds in Tomato Fields NY/T 1464. 20--2007
Published by China Agriculture Press
[Building 18, Maiguangdian Street, Chaozuo District, Beijing (Ministry of Administration Code: 100025
Website: www, ccep. com. cu)
Printed by China Agricultural Publishing House
Published by Beijing Xinhua Bookstore
Distributed by Xinhua Bookstores in various places
#880mm×:230mm 1/16
First edition in March 2008
Printing sheet 0.75
Number of words? words
Second printing in Beijing in March 2008
Book number: 16209:2577
Print run: 1503 copies
Price: 10.00 yuan
Copyright reserved
Infringements must be investigated
Report phone: (010) 650058943 Calculation method of drug efficacy
The number of root surveys, the last survey of pre-sowing or post-sowing pre-seedling application and post-seedling application, the control effect is calculated according to formula (1). For post-seedling application except the last survey, the control effect is calculated according to formula (2), the unit is fraction (%). The calculation result is rounded to two decimal places. E-XX×100
E=Xx100
Where:
EControl effect:
X. ——Number of live weeds (or fresh weight) in the blank control area: X:—Number of remaining weeds (or fresh weight) in the treatment area; X,“—Number of weed plants in the treatment area before drug application; X—Number of remaining weed nodes in the treatment area after drug application
....(13
.. (2)
NY/T 1464.20—2007Www.bzxZ.net
4.4 Crop Survey
4.4.1 Survey Methods
Observe the effects of pesticides on tomato growth and record the types and extent of the pesticide damage. The following requirements can be recorded: a) Record the time and symptoms of the pesticide damage in detail (such as growth inhibition, chlorosis, dead spots, deformities, etc.). b) Observe the interaction between pesticide damage and adverse factors (cultivation methods, lodging, pests and diseases, long-term high temperature or freezing damage, etc.).
For example If the fruit damage can be counted or measured, it is expressed in absolute values, such as plant height, stem thickness, degree of expansion, number of branches, number of fruits and number of deformed fruits. The number of plants measured should be no less than 15 per plot. In other cases, the degree and frequency of drug damage can be estimated by the following two methods: 1) According to the method of drug damage classification, the drug damage of each plot is graded and scored. 2) Compare the drug treatment area with the blank control area to evaluate the percentage of drug damage: Level 1: The fruit grows normally without any symptoms of damage; Level 2: The fruit is not damaged; Slight damage, less than 10% of the damage is: Level 3: Moderate damage to tomatoes, can be recovered later - no impact on yield; Level 5: Severe damage to tomatoes, difficult to recover, resulting in low yield; Level 5: Severe damage to tomatoes, cannot be recovered - resulting in obvious low yield or no yield. 4.4.2 Number of surveys
Observe 5, 1, and 5 days after application to see if there is any damage. If there is any damage, the symptoms and degree of damage should be recorded in detail, and the time required for tomatoes to resume normal growth should be observed and recorded, and the yield should be measured. 4.5 Side effect observation ||tt ||Record the effects of the test agent on non-target organisms. If other agents are used to control pests and non-target weeds, the accurate data of the application of such agents should be recorded (such as agent name, application time, application dosage, etc.): 4.6 Tomato yield
Tomatoes are a vegetable variety harvested in batches, and the difference between individual tomato plants is relatively large, so each time the fruit yield is measured, all tomato plants in the plot should be included. Generally, they are harvested every 5 to 7 days until the harvest is completed. The total yield of the plot is the sum of the yields of each batch, usually in kg/ hm2 indicates.
If required, the quality of the pesticide should be graded according to the relevant standards and relevant regulations. 5 Statistical analysis
The obtained data were statistically analyzed by using the biostatistical method (DMRT method). 6 Results and report writing
Analyze and evaluate the results and issue a formal test report. If the agent shows signs of long-lasting effect in the test, a post-crop safety test should be carried out.
NY/T 1464.20-2007
Agricultural Industry Standard of the People's Republic of China
Guidelines for Field Efficacy Tests of Pesticides
Part 2: Herbicides for Control of Weeds in Tomato Fields NY/T 1464. 20--2007
Published by China Agriculture Press
[Building 18, Maiguangdian Street, Chaozuo District, Beijing (Ministry of Administration Code: 100025
Website: www, ccep. com. cu)
Printed by China Agricultural Publishing House
Published by Beijing Xinhua Bookstore
Distributed by Xinhua Bookstores in various places
#880mm×:230mm 1/16
First edition in March 2008
Printing sheet 0.75
Number of words? words
Second printing in Beijing in March 2008
Book number: 16209:2577
Print run: 1503 copies
Price: 10.00 yuan
Copyright reserved
Infringements must be investigated
Report phone: (010) 650058943 Calculation method of drug efficacy
The number of root surveys, the last survey of pre-sowing or post-sowing pre-seedling application and post-seedling application, the control effect is calculated according to formula (1). For post-seedling application except the last survey, the control effect is calculated according to formula (2), the unit is fraction (%). The calculation result is rounded to two decimal places. E-XX×100
E=Xx100
Where:
EControl effect:
X. ——Number of live weeds (or fresh weight) in the blank control area: X:—Number of remaining weeds (or fresh weight) in the treatment area; X,“—Number of weed plants in the treatment area before drug application; X—Number of remaining weed nodes in the treatment area after drug application
....(13
.. (2)
NY/T 1464.20—2007
4.4 Crop Survey
4.4.1 Survey Methods
Observe the effects of pesticides on tomato growth and record the types and extent of the pesticide damage. The following requirements can be recorded: a) Record the time and symptoms of the pesticide damage in detail (such as growth inhibition, chlorosis, dead spots, deformities, etc.). b) Observe the interaction between pesticide damage and adverse factors (cultivation methods, lodging, pests and diseases, long-term high temperature or freezing damage, etc.).
For example If the fruit damage can be counted or measured, it is expressed in absolute values, such as plant height, stem thickness, degree of expansion, number of branches, number of fruits and number of deformed fruits. The number of plants measured should be no less than 15 per plot. In other cases, the degree and frequency of drug damage can be estimated by the following two methods: 1) According to the method of drug damage classification, the drug damage of each plot is graded and scored. 2) Compare the drug treatment area with the blank control area to evaluate the percentage of drug damage: Level 1: The fruit grows normally without any symptoms of damage; Level 2: The fruit is not damaged; Slight damage, less than 10% of the damage is: Level 3: Moderate damage to tomatoes, can be recovered later - no impact on yield; Level 5: Severe damage to tomatoes, difficult to recover, resulting in low yield; Level 5: Severe damage to tomatoes, cannot be recovered - resulting in obvious low yield or no yield. 4.4.2 Number of surveys
Observe 5, 1, and 5 days after application to see if there is any damage. If there is any damage, the symptoms and degree of damage should be recorded in detail, and the time required for tomatoes to resume normal growth should be observed and recorded, and the yield should be measured. 4.5 Side effect observation ||tt ||Record the effects of the test agent on non-target organisms. If other agents are used to control pests and non-target weeds, the accurate data of the application of such agents should be recorded (such as agent name, application time, application dosage, etc.): 4.6 Tomato yield
Tomatoes are a vegetable variety harvested in batches, and the difference between individual tomato plants is relatively large, so each time the fruit yield is measured, all tomato plants in the plot should be included. Generally, they are harvested every 5 to 7 days until the harvest is completed. The total yield of the plot is the sum of the yields of each batch, usually in kg/ hm2 indicates.
If required, the quality of the pesticide should be graded according to the relevant standards and relevant regulations. 5 Statistical analysis
The obtained data were statistically analyzed by using the biostatistical method (DMRT method). 6 Results and report writing
Analyze and evaluate the results and issue a formal test report. If the agent shows signs of long-lasting effect in the test, a post-crop safety test should be carried out.
NY/T 1464.20-2007
Agricultural Industry Standard of the People's Republic of China
Guidelines for Field Efficacy Tests of Pesticides
Part 2: Herbicides for Control of Weeds in Tomato Fields NY/T 1464. 20--2007
Published by China Agriculture Press
[Building 18, Maiguangdian Street, Chaozuo District, Beijing (Ministry of Administration Code: 100025
Website: www, ccep. com. cu)
Printed by China Agricultural Publishing House
Published by Beijing Xinhua Bookstore
Distributed by Xinhua Bookstores in various places
#880mm×:230mm 1/16
First edition in March 2008
Printing sheet 0.75
Number of words? words
Second printing in Beijing in March 2008
Book number: 16209:2577
Print run: 1503 copies
Price: 10.00 yuan
Copyright reserved
Infringements must be investigated
Report phone: (010) 65005894
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