This standard is based on the "National Clinical Laboratory Operation Procedure (Second Edition)" issued by the Ministry of Health, with reference to the standards of the National Committee for Clinical Laboratory Standards (NCCLS) of the United States, and is formulated in combination with China's national conditions and the actual situation and requirements of the health system. This standard specifies the test process for manufacturers and users to test the quality of commercial microbiological culture media. The culture media that manufacturers must test but users do not need to test are listed in Table 1, including the quality control strains required for testing each group of culture media and the acceptable expected results. The culture media that users must test are listed in Table 2. The incubation environment and time are applicable to the listed accused strains, but it does not mean that these conditions are recommended for clinical specimens. WS/T 232-2002 Quality Inspection Procedure for Commercial Microbiological Culture Media WS/T232-2002 Standard download decompression password: www.bzxz.net

Health Industry Standard of the People's Republic of China
WS/T232—2002
Performance standard of quality assurance for commerciallypreparedmicrobiologicalculturemedia2002-04-20 Issued
Issued by the Ministry of Health of the People's Republic of China
2002-07-01 Implementation
WS/T232-2002
This standard is formulated on the basis of the National Clinical Laboratory Operation Procedure (Second Edition) issued by the Ministry of Health, with reference to the standards of the National Committee for Clinical Laboratory Standards (NCCIS) of the United States, and in combination with the actual conditions and requirements of China's health system. This standard shall be implemented from July 1, 2002. This standard is proposed by the Medical Administration Department of the Ministry of Health.
The drafting unit of this standard: Peking University People's Hospital. The main drafters of this standard: Zhang Zheng, Yang Jing, Zhao Xiaotao. This standard is entrusted by the Ministry of Health to the Ministry of Health Clinical Laboratory Center for interpretation. Health Industry Standard of the People's Republic of China
Performance standard of quality assurance for commercially prepared microbiological culture media1Scope
WS/T232--2002
This standard specifies the test process for manufacturers and users to conduct quality inspections on commercial microbiological culture media. The culture media that manufacturers must test but users do not need to test are listed in Table 1, including the quality control strains required for testing each group of culture media and the expected acceptable results. The culture media that users must test are listed in Table 2. The incubation environment and time are suitable for the listed quality control strains, but it does not mean that these conditions are recommended for clinical specimens.
2Tests performed by manufacturers
2.1 Source of quality control strains
All quality control strains in Table 1 and Table 2 are from ATCC (American Type Culture Collection). Commercially derived strains from ATCC or standard strains of various levels stored in my country's national bacterial strain bank can also be used. The strains listed in Table 1 and Table 2 are the minimum requirements that should be included in the test combination for evaluating the listed culture media. Manufacturers may also use additional strains on purpose.
Culture medium
Anaerobic sheep blood agar culture
Anaerobic broth (containing sheep blood):
Blood agar-CAMP test
Blood agar-selective sheep blood
Agar culture medium
Aerobic or carbon dioxide
Carbon dioxide
Commercial culture medium for manufacturer quality assurance standard incubation time
24h~48h||t t||24h~48h
Quality control strain approved by the Ministry of Health of the People's Republic of China on April 20, 2002 (ATCC No.)
Bacteroides fragilis (25285)
Clostridium perfringens (13124)
Fusobacterium spp. (25586)
Peptococcus anaerobicus (27337)
Streptococcus pyogenes (19615)||tt ||Streptococcus pneumoniae (6305)
Staphylococcus aureus (25923)
Escherichia coli (25922)
Staphylococcus aureus (33862 or
25923)
Streptococcus agalactiae (12386)
Streptococcus pyogenes (19615)
Streptococcus pyogenes (19615)
Streptococcus edema (6305)
Staphylococcus aureus (25923)
Proteus mirabilis (12453)
Expected results
Growth, beta hemolysis
Growth, beta hemolysis
Growth, alpha hemolysis
Negative reaction (no arrowhead formation)
Positive reaction (arrowhead area formed)
Negative reaction (no arrowhead formation) Head formation)
Growth, beta hemolysis
Growth, alpha hemolysis
Inhibition (partial)
2002-07-01Implementation
Culture medium
Phenylethanol agar
Blood culture medium
CLED agar
CYE/BCYE agar
Eosin-methylene blue culture medium
Hektoen| |tt||MacConkey agar
enteric
Mannitol high salt agar
Mycobacterium Sheng agar culture
Carbon dioxide
Anaerobic (no ventilation)
Aerobic (ventilation)
WS/T232-2002
Table 1 (continued)
Incubation time
24h~48h
Within 5 days
Within 5 days
24h~48h
48h~72h
Quality control strain (ATCC number)
Streptococcus pyogenes (19615)
Staphylococcus aureus (25923)
Proteus mirabilis (12453)
Bacteroides fragilis (25285)
Streptococcus pneumoniae (6305)
Pseudomonas aeruginosa (27853)
Streptococcus pneumoniae (6305)
Escherichia coli (25922)
Proteus vulgaris (8427)
Staphylococcus aureus (25923)
Legionella pneumophila (33152)
Legionella bozemanii (33217)
Salmonella typhimurium (14028)
Shigella sonnei (9290)
Escherichia coli (25922)
Salmonella enterica (14028)
Escherichia coli (25922)
Enterococcus faecalis (29212)
Salmonella enterica (14028)
Shigella citrinum (12022)
Enterococcus faecium (29212)|| tt||Escherichia coli (25922)
Escherichia coli (25922)
Proteus mirabilis (12453)
Salmonella enterica (14028)
Enterococcus faecalis (29212)
Staphylococcus aureus (25923)
24h~48h
Up to 21 days
Staphylococcus epidermidis ( 12228）
Proteus mirabilis(12453)
H37Ra Mycobacterium tuberculosis
(25177)
Group 1 Mycobacterium tassana
(12478)
Group 1 Mycobacterium rubrum
(19981)
Group Mycobacterium intracellulare
(13950)
Group N sporadic Mycobacterium
(6841)
Escherichia coli (25922)
Expected results
Inhibition (partial)
Growth, yellow center
Growth, purple ridges, inhibition of spreading (partial) growth, uneven dark yellow
Growth, yellow-green fluorescence
Growth, blue-white fluorescence
Subculture growth
Subculture growth (may be inhibited by selenite medium)
inhibited by passage (partial to complete)
growth, colorless to amber colonies
growth, blue-green colonies inhibited (partial) growth, blue to cyan colonies, cyan growth, green to cyan colonies
inhibited (partial, yellow colonies)
inhibited (partial to complete, yellow colonies) growth, pink colonies
growth, colorless colonies, inhibited clumping
(partial)
growth, colorless colonies
inhibited (partial)
growth, yellow colonies at 48h
growth, red colonies at 48h
inhibited (partial)
growth, may be inhibited
growth on selective LJ and selective
Middlebrook medium , may be inhibited (partially to completely) on selective LJ and selective Middlebrook media - selective mycobacterial media only
media
Sabouraud saxolyl succinate
SS agar
selective mycoplasma cultures
selective media for enterococci,
(containing azide)
selective media for enterococci,
(without azide)
thioglycollate media
with or without indicator
thioglycollate media,
enriched with vitamin K and hemin (BHI and
XLD agar
Note 1 Unless otherwise specified, the temperature is 35°C. WS/T2322002
Table 1 (end)
Incubation time
Up to 7 days,
25℃~35℃
Up to 7 days,
24h and 48h
24h and 48h
(Plug the bottle cap)
(Plug the bottle stopper)
24h48h
Quality Control strains (ATCC number)
Candida albicans (60193 or
10231)
Trichophyton tinea (9533)
Salmonella typhimurium (14028)
Shigella flexneri (12022)
Enterococcus faecalis (29212)
Escherichia coli (25922)
A.niger(16404)
Candida albicans(10231)
Trichophyton polyphyon(9533)
Escherichia coli(25922)
Enterococcus faecalis(29212)
Streptococcus pyogenes(19615)
Escherichia coli(25922)
Enterococcus pseudomallei(29212)
Streptococcus pyogenes(19615)
Bacteroides fragilis(25285)
Staphylococcus aureus(25923)
Peptococcus anaerobicus (27337)
Bacteroides vulgaris (8482)
Clostridium perfringens (13124)
Escherichia coli (25922)
Staphylococcus aureus (25923)
Salmonella enterica serovar Typhimurium (14028)
Shigella flexneri (12022)
Enterococcus faecalis (29212)
Escherichia coli (25922)
Note 2ATCC is a registered trademark of the American Center for the Study of Standardized Strains. Note 3This requirement applies to manufacturers and users do not have to perform testing. Growth
Expected results
Growth, colorless colonies with or without black core
Growth, colorless colonies
Inhibition (complete)
Inhibition (partial to complete) Pink to rose red colonies with precipitate
Inhibited on medium containing actinomycin
Inhibited (partial to complete)
Inhibited on medium containing tadalafil
Inhibited (partial to complete)
Growth, colonies turn black
Inhibited (partial to complete)
Inhibited ( Part) - Colorless colonies on bile agar
Growth, blackening around colonies
Bonnet (partial to complete)
Growth, red colonies with black core
Growth, red colonies
Inhibition (partial)
Inhibition (partial to complete, yellow to red colonies)
Cultivation medium
Campylobacter flag agar
Selective medium for pathogenic Neisseria
Reduce oxygen,
WS/T 232--2002
Table 2 User quality inspection of commercial culture media Incubation time
Add carbon dioxide,
Carbon dioxide
Note 1 Unless otherwise specified, the temperature is 35°C. 48h
24h~48h
Quality control strain (ATCC number)
Campylobacter jejuni (33291)
Escherichia coli (25922)
Neisseria gonorrhoeae (43069 or
43070)
Proteus mirabilis (43071)
Escherichia coli (12228)
Note 2ATCC is a registered trademark of the American Standard Strain Collection Center. Growth
Expected Results
Inhibition (partial)
Inhibition (partial), only for media containing trimethoprim
Inhibition (partial)
2.2 Storage of Quality Control Strains
The storage process for strains used for quality control purposes must be standardized to reduce the chance of contamination or changes in growth characteristics. This can be achieved by using frozen stocks or using standardized strains from commercial sources. The following procedures (Sections 2.3 and 2.4) can be used to prepare quality control strains of rapidly growing bacteria, such as Staphylococci, most Tensorcocci, Enterobacteriaceae, and Pseudomonas aeruginosa. These procedures must be modified for the preparation and use of Neisseria, Haemophilus, Mycobacterium, and fungi. If the strain is from a commercial source, use it according to the manufacturer's instructions. 2.3 Preparation of Frozen Strains
2.3.1 Quality control strains are generally prepared from lyophilized strains from ATCC and revived according to ATCC instructions. 2.3.2 Use a sterile loop to streak the revived bacterial suspension onto two or three TSA agars with 5% sheep blood, and incubate for 18h to 24h at a suitable environment and temperature (see Table 1). 2.3.3 After incubation, check the purity by colony morphology. Perform biochemical tests if necessary. Suspend sufficient bacteria in 50ml to 100ml of cryopreservation medium. The cryopreservation medium can be sterile defibrinated sheep blood, rabbit blood, skim milk or TSB, which contains 10% to 15% glycerol at a final concentration. If these frozen strains are to be used to inoculate the test medium, the turbidity of the suspension should be adjusted to 0.5 McFarland standard (1 to 2×10°CFU/mL) before freezing. The suspension of gonorrhea bacteria should be adjusted to 1.0 Jaffarland standard. The bacterial suspension must contain sufficient viable bacteria to continue to be subcultured after a freeze-thaw cycle. 2.3.4 Dispense a small amount (0.5mL~1.0mL) of the bacterial suspension into small sterile glass or plastic bottles. Prepare enough quality control culture bottles to last for one year. Check purity before quality control testing. 2.3.5 Store the vials at -50C or lower for future use. The vials can be stored in the gas phase of an ultra-low temperature freezer or liquid nitrogen tank. The strain can be stored indefinitely at temperatures below -70C, but can only be stored for one year at -50℃~-70℃, and should not be stored at temperatures above -50C.
2.3.6 When needed, take a vial of quality control culture from the refrigerator or liquid nitrogen tank and thaw it quickly, and it can be used for testing or preparing working quality control culture. Secondary cultures can also be used for testing, and single colonies can be obtained by inoculating a solid culture medium. After incubation, use a single colony for testing or inoculating agar slants or plates for use as the main working quality control strain. Unused bacterial suspension should be discarded and not reused after freezing.
2.4 Working Quality Control Cultures
2.4.1 The quality control culture should be replaced after no more than three generations of frozen quality control culture. Excessive passages increase the chance of phenotypic changes. 2.4.2 To prepare working quality control cultures, inoculate an agar slant or plate with the frozen quality control culture and incubate overnight or until sufficient volume is obtained. These cultures on working agar slant or plates should be stored at 2C to 8°C, or at room temperature, for four weeks. After further passage, the culture should be checked for purity and typical colony morphology. 2.4.3 Working frozen cultures can also be prepared from frozen quality control cultures using the steps described in Section 2.3. Either the original stock bottle or the working quality control strain can be used to inoculate the test medium.
2.4.4 The working quality control strain of anaerobic bacteria should be stored in meat culture medium or other suitable anaerobic broth medium, or frozen preservation 4
strains should not be used agar slant or plate. 2.5 Culture medium detection process
WS/T232-2002
2.5.1 The working quality control strain is transferred to the culture medium, and after incubation, 3~5 individual colonies are suspended in a small amount of sterilized TSB and incubated for 4h~5h. Use a spectrophotometer to adjust the turbidity to 0.5 McFarland standard or 0.08~0.1 at 625nm absorbance. Alternatively, prepare the bacterial suspension directly from the overnight growth and adjust the turbidity in the same way. Regardless of which method is used to determine the turbidity of the inoculum, the colony count should reach (1~2)×10°CFU/mL. If the frozen culture has been adjusted to this range, the dilution can be prepared directly from the thawed culture. In order to obtain bacteria with appropriate vitality after freezing, for microorganisms that are sensitive to freezing (such as Neisseria gonorrhoeae), the count during freezing should reach (3-6)×10°CFU/mL (1.0 McFarland). 2.5.2 To test the nutritional capacity of the culture medium, dilute the basic bacterial suspension 1:100 with saline or purified water, and streak inoculate with 10μL inoculation loop or 0.01mL of the diluted suspension on each test culture medium to achieve a concentration of (12)×10*CFU/plate. If this operation does not ensure the formation of single colonies on a specific test culture medium, the inoculum should be diluted 10 times again. 2.5.3 To test the inhibitory capacity of the selective culture medium, dilute the basic bacterial suspension 1:10 with saline or purified water, and streak inoculate with 10μL loop or 0.01mL of the diluted suspension on each test culture medium to achieve a concentration of (1-2)×105CFU/plate. To prevent excessive growth on the selective culture medium, it may be necessary to dilute the inoculum 10 times. 2.5.4 To test the growth effect of the culture tube, inoculate with a 10μL loop or 0.01mL of the basic bacterial suspension. If necessary, adjust the concentration of the inoculum.
2.5.5 Incubate the inoculated culture medium under the conditions of Table 2, normally at 35°C for 18h-24h or 42h-48h. Chocolate agar and selective culture medium for the isolation of pathogenic Neisseria must be incubated in a 5%-10% carbon dioxide environment, and the results are checked at 18h-24h and 42h-48h. For anaerobic bacteria, it is usually cultured in an anaerobic environment rich in carbon dioxide for at least 48h. For Campylobacter agar, incubate in a microaerobic environment rich in carbon dioxide at 42°C for 48h. 2.6 If Explanation
If the test strain grows fully in the culture medium listed in Table 1 and forms a typical colony morphology, the culture medium is considered to be satisfactory. For selective culture media, some bacteria are inhibited, while the desired bacteria grow fully. In some cases, the appropriate color or hemolysis reaction shown in Table 1 must be produced.
2.7 Reporting quality control data to the user
2.7.1 Culture media listed in Table 1
The manufacturer should indicate on the label, technical manual or other documents that the tested product meets this standard. The manufacturer should also indicate other tests such as sterility test and pH determination.
2.7.2 Culture media not listed in Table 1
The manufacturer must report to the user the special quality control steps required, including: the strain used for testing, pH, evaluation of operating standards, the response of the strain to be tested, and evaluation of contamination. The test results of each batch do not need to be reported one by one, but proof should be provided to show that the quality control standards of the product are met. The batch number and shelf life should be indicated in the report.
Culture media not listed in Table 1 should be tested by the user. 2.8 Transportation and storage
Culture media should be prevented from excessive water loss during transportation and should be protected and kept warm. Manufacturers should consider transportation and storage conditions when setting expiration dates.
3 Tests to be performed by the user
3.1 Operational tests
As stated above, all user-prepared culture media should be tested in accordance with the above regulations. For culture media not listed in Table 1 and the following culture media, namely Campylobacter agar and culture media for selective isolation of pathogenic Neisseria (listed in Table 2), the user must test each batch of culture media. Users who obtain culture media distributed from other places in non-commercial form should also follow the rules in this document. Manufacturers (laboratories preparing culture media) should also perform quality tests applicable to the merchant. 3.2 Other quality assurance standards
Users of commercial culture media must check each batch of culture media for: cracks in the plate, uneven filling, cracks in the culture medium, hemolysis (blood agar), freezing, excessive bubbles or spots, and contamination. 3.3 Reporting of defects
If the culture media is found to be defective, the laboratory should record the results and notify the manufacturer. These include: failure to grow, failure to produce colonies of the expected size, or evidence of contamination, drying, or cracking. The manufacturer should have appropriate corrective actions, which should also be recorded in the laboratory. These situations do not necessarily require a change of culture medium. The user can perform additional tests to identify the cause and correct it. Table 3 Comparison of Chinese and English names of culture media
Chinese name of culture medium
Anaerobic broth
Anaerobic sheep blood agar culture medium
Blood agar-CAMP test
Blood agar-selective sheep blood agar culture medium
Blood culture mediumbzxZ.net
Campylobacter agar
CLED agar
CYE/BCYE agar
Eosin methylene blue culture medium
Hektoen enteric agar
MacConkey agar
Mannitol high salt agar
Mycobacterium agar
Lysostearyl alcohol agar
Sabourgastrine glucose medium
SS agar
Selective medium for enterococci (containing azide)Selective medium for enterococci (without azide)Selective medium for pathogenic Neisseria speciesSelective medium for mycoplasma
Medium, enriched with vitamin K and oxidized
Glycogen acetate
Heme
Medium, with or without indicator
Thioglycolate
Tube media (BHI and TSB)
XLD agar
anaerobic broths
Medium English name
anaerobic sheep blood and laked blood agar mediablood agar-CAMP test
blood agar-selective sheep blood agar mediablood eulture media
campylobacter agar
CLED agar aerobic
CYE/BCYE agar aerobie
eosin methylene blue media
Hektoen enteric agar aerobicmacconkey'agar aerobic | | tt | Neisseria speciesselective mycology media
thioglycolate medium, enrichedwithvitaminKandhemin aerobicthioglycolate medium.with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)685335332 Media not listed in Table 1
The manufacturer must report to the user any special quality control steps required, including: the strain used for testing, pH, evaluation of operating standards, the response of the strain to be tested, and evaluation of contamination. The test results of each batch do not need to be reported individually, but proof should be provided that the quality control standards of the product are met. The batch number and shelf life should be stated in the report.
Media not listed in Table 1 should be tested by the user. 2.8 Transportation and storage
Media should be protected from excessive water loss during transportation and should be protected and kept warm. The manufacturer should consider transportation and storage conditions when establishing the expiration date.
3 Tests required by the user
3.1 Operational tests
As mentioned above, all media prepared by the user should be tested in accordance with the above regulations. For media not listed in Table 1 and the following media, namely Campylobacter agar and media for selective isolation of pathogenic Neisseria (listed in Table 2), the user must test each batch of media. Users of culture media distributed from other sources in non-commercial form should also follow the rules in this document. Producers (laboratories preparing culture media) should also perform quality checks applicable to the vendor. 3.2 Other quality assurance standards
Users of commercial culture media must check each batch of culture media for: cracks in the plate, uneven filling of the plate, cracks in the culture media, hemolysis (blood agar), freezing, excessive bubbles or spots, and contamination. 3.3 Defect reporting
If the culture media is found to be defective, the laboratory should record the results and notify the manufacturer. These include: failure to grow, failure to produce colonies of the expected size, or evidence of contamination, drying, or cracking. The manufacturer should have appropriate corrective measures, which should also be recorded in the laboratory. These situations do not necessarily require a change of culture media, and the user can perform additional tests to identify the cause and correct it. Table 3 Comparison of Chinese and English names of culture media
Chinese name of culture media
Anaerobic broth
Anaerobic sheep blood agar medium
Blood agar-CAMP test
Blood agar-selective sheep blood agar medium
Blood culture medium
Campylobacter agar
CLED agar
CYE/BCYE agar
Eosin-methylene blue medium
Hektoen enteric agar
MacConkey agar
Mannitol high salt agar
Mycobacterium agar
Lysostearyl alcohol agar
Sabourgastrine glucose medium
SS agar
Selective medium for enterococci (containing azide)Selective medium for enterococci (without azide)Selective medium for pathogenic Neisseria speciesSelective medium for mycoplasma
Medium, enriched with vitamin K and oxidized
Glycogen acetate
Heme
Medium, with or without indicator
Thioglycolate
Tube media (BHI and TSB)
XLD agar
anaerobic broths
Medium English name
anaerobic sheep blood and laked blood agar mediablood agar-CAMP test
blood agar-selective sheep blood agar mediablood eulture media
campylobacter agar
CLED agar aerobic
CYE/BCYE agar aerobie
eosin methylene blue media
Hektoen enteric agar aerobicmacconkey'agar aerobic | | tt | Neisseria speciesselective mycology media
thioglycolate medium, enrichedwithvitaminKandhemin aerobicthioglycolate medium.with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)685335332 Media not listed in Table 1
The manufacturer must report to the user any special quality control steps required, including: the strain used for testing, pH, evaluation of operating standards, the response of the strain to be tested, and evaluation of contamination. The test results of each batch do not need to be reported individually, but proof should be provided that the quality control standards of the product are met. The batch number and shelf life should be stated in the report.
Media not listed in Table 1 should be tested by the user. 2.8 Transportation and storage
Media should be protected from excessive water loss during transportation and should be protected and kept warm. The manufacturer should consider transportation and storage conditions when establishing the expiration date.
3 Tests required by the user
3.1 Operational tests
As mentioned above, all media prepared by the user should be tested in accordance with the above regulations. For media not listed in Table 1 and the following media, namely Campylobacter agar and media for selective isolation of pathogenic Neisseria (listed in Table 2), the user must test each batch of media. Users of culture media distributed from other sources in non-commercial form should also follow the rules in this document. Producers (laboratories preparing culture media) should also perform quality checks applicable to the vendor. 3.2 Other quality assurance standards
Users of commercial culture media must check each batch of culture media for: cracks in the plate, uneven filling of the plate, cracks in the culture media, hemolysis (blood agar), freezing, excessive bubbles or spots, and contamination. 3.3 Defect reporting
If the culture media is found to be defective, the laboratory should record the results and notify the manufacturer. These include: failure to grow, failure to produce colonies of the expected size, or evidence of contamination, drying, or cracking. The manufacturer should have appropriate corrective measures, which should also be recorded in the laboratory. These situations do not necessarily require a change of culture media, and the user can perform additional tests to identify the cause and correct it. Table 3 Comparison of Chinese and English names of culture media
Chinese name of culture media
Anaerobic broth
Anaerobic sheep blood agar medium
Blood agar-CAMP test
Blood agar-selective sheep blood agar medium
Blood culture medium
Campylobacter agar
CLED agar
CYE/BCYE agar
Eosin-methylene blue medium
Hektoen enteric agar
MacConkey agar
Mannitol high salt agar
Mycobacterium agar
Lysostearyl alcohol agar
Sabourgastrine glucose medium
SS agar
Selective medium for enterococci (containing azide)Selective medium for enterococci (without azide)Selective medium for pathogenic Neisseria speciesSelective medium for mycoplasma
Medium, enriched with vitamin K and oxidized
Glycogen acetate
Heme
Medium, with or without indicator
Thioglycolate
Tube media (BHI and TSB)
XLD agar
anaerobic broths
Medium English name
anaerobic sheep blood and laked blood agar mediablood agar-CAMP test
blood agar-selective sheep blood agar mediablood eulture media
campylobacter agar
CLED agar aerobic
CYE/BCYE agar aerobie
eosin methylene blue media
Hektoen enteric agar aerobicmacconkey'agar aerobic | | tt | Neisseria speciesselective mycology media
thioglycolate medium, enrichedwithvitaminKandhemin aerobicthioglycolate medium.with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)685335333 Defect Report
If the culture medium is found to be defective, the laboratory should record the result and notify the manufacturer. These include: failure to grow, failure to produce colonies of the expected size, or evidence of contamination, drying, or cracking. The manufacturer should have appropriate improvement measures, which should also be recorded in the laboratory. These situations do not necessarily require changing the culture medium. The user can perform additional tests to find out the cause and correct it. Table 3 Comparison of Chinese and English names of culture media
Chinese name of culture medium
Anaerobic broth
Anaerobic sheep blood agar culture medium
Blood agar-CAMP test
Blood agar-selective sheep blood agar culture medium
Blood culture medium
Campylobacter agar
CLED agar
CYE/BCYE agar
Eosin methylene blue culture medium
Hektoen enteric agar
MacConkey agar
Mannitol high salt agar
Mycobacterium agar
Lysostearyl alcohol agar
Sabourgastrine glucose medium
SS agar
Selective medium for enterococci (containing azide)Selective medium for enterococci (without azide)Selective medium for pathogenic Neisseria speciesSelective medium for mycoplasma
Medium, enriched with vitamin K and oxidized
Glycogen acetate
Heme
Medium, with or without indicator
Thioglycolate
Tube media (BHI and TSB)
XLD agar
anaerobic broths
Medium English name
anaerobic sheep blood and laked blood agar mediablood agar-CAMP test
blood agar-selective sheep blood agar mediablood eulture media
campylobacter agar
CLED agar aerobic
CYE/BCYE agar aerobie
eosin methylene blue media
Hektoen enteric agar aerobicmacconkey'agar aerobic | | tt | Neisseria speciesselective mycology media
thioglycolate medium, enrichedwithvitaminKandhemin aerobicthioglycolate medium.with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)685335333 Defect Report
If the culture medium is found to be defective, the laboratory should record the result and notify the manufacturer. These include: failure to grow, failure to produce colonies of the expected size, or evidence of contamination, drying, or cracking. The manufacturer should have appropriate improvement measures, which should also be recorded in the laboratory. These situations do not necessarily require changing the culture medium. The user can perform additional tests to find out the cause and correct it. Table 3 Comparison of Chinese and English names of culture media
Chinese name of culture medium
Anaerobic broth
Anaerobic sheep blood agar culture medium
Blood agar-CAMP test
Blood agar-selective sheep blood agar culture medium
Blood culture medium
Campylobacter agar
CLED agar
CYE/BCYE agar
Eosin methylene blue culture medium
Hektoen enteric agar
MacConkey agar
Mannitol high salt agar
Mycobacterium agar
Lysostearyl alcohol agar
Sabourgastrine glucose medium
SS agar
Selective medium for enterococci (containing azide)Selective medium for enterococci (without azide)Selective medium for pathogenic Neisseria speciesSelective medium for mycoplasma
Medium, enriched with vitamin K and oxidized
Glycogen acetate
Heme
Medium, with or without indicator
Thioglycolate
Tube media (BHI and TSB)
XLD agar
anaerobic broths
Medium English name
anaerobic sheep blood and laked blood agar mediablood agar-CAMP test
blood agar-selective sheep blood agar mediablood eulture media
campylobacter agar
CLED agar aerobic
CYE/BCYE agar aerobie
eosin methylene blue media
Hektoen enteric agar aerobicmacconkey'agar aerobic | | tt | Neisseria speciesselective mycology media
thioglycolate medium, enrichedwithvitaminKandhemin aerobicthioglycolate medium.with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)68533533with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)68533533with or without indicator aerobictubed media(BHI and tryptic soy broth)XLD(xylose lysine desoxycholate) agar aerobicWS/T232-2002
People's Republic of China Health
Industry Standard
Quality Inspection Procedure for Commercial Microbiological Culture MediaWS/T232--2002
Published by China Standards Press
No.16,Sanlihebei Street,Fuxingmenwai,Beijing
Postal Code:100045
Tel:6852394668517548
China Printed by Qinhuangdao Printing Factory of China Standard Press Distributed by Beijing Publishing House of Xinhua Bookstore Sold by Xinhua Bookstores in various places Format 880×12301/16 Printing sheet 3/4 Word count 15,000 words First edition in August 2002 First printing in August 2002 Print run 1-800
Website bzcbs.com
Keri 613-393
Infringements will be investigated
Copyright reserved
Report phone number: (010)68533533
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