This standard specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of pear scab (Venturia pirina). This standard applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of pear scab for registration. Other field efficacy tests shall refer to this standard. GB/T 17980.24-2000 Guidelines for field efficacy tests of pesticides (I) Fungicides for the control of pear scab GB/T17980.24-2000 Standard download decompression password: www.bzxz.net
This standard specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of pear scab (Venturia pirina). This standard applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of pear scab for registration. Other field efficacy tests shall refer to this standard.

GB/T 17980.24—2000
Field efficacy test is one of the important contents of pesticide registration management and an important technical basis for the formulation of pesticide product labels. Labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Pear scab is one of the important diseases of pear trees in my country, and fungicides are often used for prevention and control in production. In order to determine the optimal field use concentration of agents for the prevention and control of pear scab, test the effects of agents on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and rational use technology of fungicide registration, this standard is specially formulated. This standard is one of the standards in the Guidelines for Field Efficacy Trials of Pesticides (I), but it is an independent standard. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This standard is drafted and interpreted by the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this standard are Wu Xinping, Liu Naizhi, Yan Baogen and Zhu Qinghua. 1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I)
Fungicides against scab of pear
Pesticide--
Guidelines for the field efficacy trials (I)Fungicides against scab of pear:GB/T 17980.24—2000
This standard specifies the methods and basic requirements for field efficacy trials of fungicides against pear scab (Venturia pinina). This standard is applicable to field efficacy trials and efficacy evaluation of fungicides for registration of fungicides against pear scab. Other field efficacy tests shall be carried out in accordance with this standard.
2 Test conditions
2.1 Test objects, selection of crops and varieties The test object is black spot.
The test crop is pear. Select pear varieties susceptible to black spot, and select fruit trees of the same variety, age and tree vigor. Record the name of the fruit tree variety.
2.2 Environmental conditionsbzxZ.net
The tillage conditions (soil type, fertilization conditions, tree age, plant spacing) of all test plots must be uniform and consistent and in accordance with local scientific agricultural practices (GAP). Record the irrigation time, quantity and method. 3 Test design and arrangement
3.1 Chemicals
3.1.1 Test chemicals
Indicate the trade name/code, Chinese name, generic name, dosage form content and manufacturer of the chemical. The test chemical treatment shall be no less than three doses or the dosage specified in the agreement (test agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control drugs
Control drugs must be registered products that have been proven to have good efficacy in practice. The type and mode of action of control drugs should be similar to those of test drugs and the local commonly used dosage should be used. Special cases can be determined according to the purpose of the test. 3.2 Plot arrangement
3.2.1 Plot arrangement
The plots of test drugs, control drugs and blank controls are arranged in random blocks. Special cases should be explained. 3.2.2 Plot area and repetition
Plot area: no less than 2 mature fruit trees per plot. Approved by the State Administration of Quality and Technical Supervision on February 1, 2000 112
Implemented on May 1, 2000
Number of repetitions: at least 4 repetitions.
3.3 Application method
3.3.1 Application method
GB/T17980.24—2000
Perform according to the requirements of the agreement and the label instructions. The application of pesticides should be adapted to local scientific agricultural practices. 3.3.2 Use of equipment
Select the equipment commonly used in production, and record all the information on the type of equipment used and the operating conditions (operating pressure, nozzle diameter). The application of pesticides should ensure accurate dosage and uniform distribution. The deviation of the dosage should be recorded if it exceeds ±10%. 3.3.3 Application time and frequency
Perform according to the requirements of the agreement and the label instructions, and determine the number and interval of application according to the epidemic law of the disease and the distribution of rainy days. Record the number of applications, application date and the growth period of fruit trees. 3.3.4 Use dosage and capacity
Use according to the requirements of the agreement and the dosage (concentration) indicated on the label. Usually the active ingredient content in the pesticide is expressed as g/hm2 (grams per hectare). Due to the different sizes of fruit trees, the multiple of the pesticide application should be recorded at the same time, and the amount of pesticide applied per unit area and per tree [L/hm2 or L/plant (liter/hectare or liter/plant)].
3.3.5 Data requirements for pesticides for the control of other diseases and insect pests If other pesticides are to be used, pesticides that have no effect on the test pesticides and test objects should be selected, and all test plots should be treated evenly, and they should be used separately from the test pesticides and control pesticides to minimize the interference of these pesticides. Record accurate data on the application of such pesticides.
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type, daily rainfall in mm) and temperature (daily average temperature, maximum and minimum temperature in °C) data should be obtained from the test site or the nearest meteorological station. The adverse weather factors that affect the test results during the entire test period, such as severe or long-term drought, rainstorms, cold waves, ice and so on, should be recorded.
The record of relevant meteorological data during the pathogen infection period is meaningful for the analysis results and the determination of the treatment time of the therapeutic agent.
4.1.2 Soil data
Record soil type, organic matter content, moisture (dry, wet or waterlogged), soil cover (crop residues, plastic film covering, weeds) and other data.
4.2 Survey method, time and frequency
4.2.1 Survey method
Two plants in each plot are surveyed, and each plant is sampled at five points: east, south, west, north and center. At each point, 20 leaves and 20 fruits of the current year's branches are taken for investigation.
Leaf grading method:
Grade 0: disease-free;
Grade 1: The area of ​​lesions accounts for less than 10% of the entire leaf area; Grade 3: The area of ​​lesions accounts for 11% to 25% of the entire leaf area; Grade 5: The area of ​​lesions accounts for 26% to 40% of the entire leaf area; Grade 7: The area of ​​lesions accounts for 41% to 65% of the entire leaf area; Grade 9: The area of ​​lesions accounts for more than 65% of the entire leaf area. Fruit grading method:
Grade 0: No lesions on the fruit;
Grade 1: 1~~2 lesions on each fruit;
Grade 3: 3~4 lesions on each fruit;
Grade 5: 5~6 lesions on each fruit;
GB/T 17980.24—2000
Grade 7: 7~10 lesions on each fruit, some lesions connected occupy about one-fifth of the fruit area; Grade 9: More than 10 lesions on each fruit, and the lesions connected occupy more than one-quarter of the fruit area. 4.2.2 Survey time and frequency
Survey the disease base before the first application of pesticides. After the application of pesticides, the time and frequency of the survey should be determined according to the development of the disease and the duration of the pesticide and the requirements of the agreement. Usually the last survey is conducted 10~14 days after the last application of pesticides. 4.2.3 Calculation method of drug efficacy The drug efficacy is calculated according to formula (1) and formula (2): (relative value of the number of diseased leaves (fruits) at each level) × 100 disease index - total number of leaves (fruits) investigated × 9 control effect (%) Where: CK - disease index of blank control area before application; PT - disease index of pesticide treatment area before application; CK × PT) × 100 CK × PT. If the disease base is not investigated before application, the control effect is calculated according to formula (3): CK - PT × 100 control effect (%) = 9 4.3 Direct effects on crops
·(2))
(3)
Observe whether the pesticide has any damage to the crops, and record the type and degree of damage. In addition, record the beneficial effects on the crops (such as promoting maturity, stimulating growth, etc.).
Record pesticide damage in the following ways:
a) If the pesticide damage can be measured or calculated, it should be expressed as an absolute value, such as plant height. b) In other cases, the degree and frequency of pesticide damage can be estimated by the following two methods: 1) Record the pesticide damage situation in each plot according to the pesticide damage grading method, expressed as one, ten, ten ten, ten ten ten, ten ten ten ten. Grading method of pesticide damage:
One: No pesticide damage;
Ten: Mild pesticide damage, does not affect the normal growth of crops; Ten Ten: Moderate pesticide damage, can be recovered, will not cause crop yield reduction; Ten Ten Ten: Severe pesticide damage, affects the normal growth of crops, causes a certain degree of loss in crop yield and quality; + Ten Ten Ten: Severe pesticide damage, crop growth is hindered, and crop yield and quality loss are serious. 2) Compare the pesticide treatment area with the blank control area to evaluate the percentage of pesticide damage. At the same time, the pesticide damage symptoms of crops (dwarfing, chlorosis, deformity, etc.) should be accurately described. 4.4 Impact on other organisms
4.4.1 Impact on other pests and diseases
Any impact on other pests and diseases must be recorded, including beneficial and unbeneficial impacts. 4.4.2 Impact on other non-target organisms
Record the impact of the pesticide on wild organisms and beneficial insects in the test area. 4.5 Product yield and quality
Generally not required.
5 Results
GB/T17980.24—-2000
Use Duncan's new multiple range (DMRT) method to statistically analyze the test data, and use corresponding biostatistics methods in special cases. Write a formal test report and analyze and evaluate the test results. The test report should list the original data. 15
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