This standard specifies the methods and basic requirements for field efficacy plot tests of terminalicides for the control of apple leaf spider mites. This standard applies to field efficacy plot tests and efficacy evaluation of terminalicides for the control of eggs, larvae, nymphs and adults of Panonychusu 1mi, as well as Bryobla rubrloculus and Tetranychus viennensis or other types of harmful waxes. Other field efficacy tests shall refer to this standard. GB/T 17980.7-2000 Guidelines for Field Efficacy Tests of Pesticides (I) Acaricides for the Control of Apple Spider Mites GB/T17980.7-2000 Standard download decompression password: www.bzxz.net
This standard specifies the methods and basic requirements for field efficacy plot tests of terminalicides for the control of apple leaf spider mites. This standard applies to the field efficacy plot test and efficacy evaluation of daphnicidal agents for controlling eggs, larvae, nymphs and adults of Panonychusu 1mi, Bryobla rubrloculus and Tetranychus viennensis or other types of harmful waxes. Other field efficacy tests shall be carried out in accordance with this standard.

GB/T 17980.7--2000
Field efficacy test is one of the important contents of pesticide registration management in my country, and is an important technical basis for the formulation of pesticide product labels, while labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Apple spider mite is one of the important mites that harm apple trees in my country, and acaricides are often used for prevention and control in production. In order to determine the optimal field use concentration for the prevention and control of apple spider mites, test the effects of the agent on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and rational use technology of acaricide registration, this standard is specially formulated. This standard is one of the standards in the Guidelines for Field Efficacy Trials of Pesticides (I) series, but it is an independent standard in itself. This standard is proposed and coordinated by the Ministry of Agriculture of the People's Republic of China. This standard was drafted and interpreted by the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this standard are Jiang Hui, Wang Xiaojun, Chen Jingfen, Xia Yan, Wu Shixiong and Wang Shaocheng. 369
1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I)
Pesticide--
Guidelines for the field efficacy trials ( I )-Acaricides against spidermite on appleGB/T17980.7—2000
This standard specifies the methods and basic requirements for field efficacy trials of acaricides against spidermite on apple. This standard applies to the field efficacy plot test and efficacy evaluation of acaricides for registration to control the eggs, larvae, nymphs and adults of Panonychus ulmi, Bryobia rubrioculus, Tetranychus iennensis or other types of pest mites. Other field efficacy tests shall be carried out in accordance with this standard. 2 Test conditions
2.1 Selection of test objects, crops and varieties The test objects are the eggs, larvae, nymphs and adults of Panonychus ulmi, Bryobia rubrioculus, Tetranychus iennensis or other types of pest mites. The test crop is apple, and any variety can be used. Record the variety name. 2.2 Environmental conditions
Select a large fruit orchard consisting of small fruit trees with dense leaves or fruit trees of the same variety. It is not suitable to use new orchards, nurseries and large fruit trees. The cultivation conditions (soil type, fertilizer, tillage, plant spacing) of all test plots must be consistent and in line with local scientific agricultural practice GAP).
3 Experimental design and arrangement
3.1 Agents
3.1.1 Test Agents
Indicate the trade name/code, Chinese name, generic name, dosage form content and manufacturer of the agent. The test agent treatment shall be no less than three doses or the dosage specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control Agents
The control agent must be a registered product that has been proven to have good efficacy in practice. The dosage form and mode of action of the control agent should be similar to that of the test agent and the local commonly used dosage should be used. Special circumstances may depend on the purpose of the test. 3.2 Plot Arrangement
3.2.1 Plot Arrangement
The plots of the test agent, control agent and blank control are arranged in random blocks. In areas with severe damage, the blank control area should be set outside the test area to prevent the impact on adjacent plots during application and cross contamination caused by wind. Approved by the State Administration of Quality and Technical Supervision on February 1, 2000 370
Implementation on May 1, 2000
3.2.2 Plot area and repetitionbzxZ.net
Plot area: 2 to 3 trees.
Number of repetitions: at least 4 repetitions.
3.3 Application method
3.3.1 Application method
GB/T 17980. 7--2000
Perform according to the requirements of the agreement and the instructions on the label, usually by spraying. Application should be adapted to local scientific agricultural practices. 3.3.2 Use of equipment
Use equipment commonly used in production and record all information on the type of equipment used and operating conditions (operating pressure, nozzle diameter). The dosage should be accurate and evenly distributed when applying pesticides. Any deviation of the dosage exceeding 10% of the soil should be recorded. 3.3.3 Application time and frequency
Follow the protocol requirements and label instructions. The application time usually depends on the stage of mite occurrence. There are different treatment methods for the experimental pesticides to control overwintering eggs, newly hatched mites (ovicides, larvicides) and active mites (acaricides for summer). Ovicides and larvicides: Follow the application instructions (usually only apply once). Acaricides for summer: It is advisable to apply the pesticide when the distribution is even, the mite population is dense and the number of mites is increasing (in summer, if necessary, the second application can be made after 14 days).
Acaricides for spring: The average mite population base is not less than 2 to 4 active mites per leaf. Acaricides for autumn: The average mite population base is not less than 6 to 8 active mites per leaf. Record the number of applications and the date of each application. 3.3.4 Dosage and volume
Apply the pesticide according to the agreement and the concentration indicated on the label. Usually the active ingredient content in the pesticide is expressed as mg/kg or mg/L (gram/dry gram or milligram/liter). When used for spraying, the multiple of the pesticide and the amount of liquid pesticide used per hectare (LL/hm (liter/hectare)) should be recorded at the same time. 3.3.5 Requirements for pesticide data for the prevention and control of other pests and diseases If other pesticides are used, pesticides that have no effect on the test pesticides and test objects should be selected, and all plots should be treated uniformly. They should be used separately from the test pesticides and control pesticides to minimize the interference of these pesticides. Record the accurate data of the application of such pesticides.
4 Survey, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type, daily rainfall in mm) and temperature (daily average temperature, maximum and minimum temperature in °C) data should be obtained from the test site or the nearest meteorological station. Adverse climatic factors that affect the test results during the entire test period, such as severe or long-term drought, heavy rain, ice, etc., must be recorded. 4.1.2 Soil data
Record soil type, fertility and topography, as well as irrigation conditions, soil cover and other information. 4.2 Survey methods, time and frequency
4.2.7 Survey methods
Investigate the number of active mites on 5~10 leaves of each tree in each plot in the east, west, south, north and center, and indicate the type of leaves taken. Use a handheld magnifying glass to examine the leaves and record the number of active mites. The number of eggs should be recorded separately. Ovicide should be checked for eggs at the bud. 4.2.2 Survey time and frequency
Survey the insect population base before treatment and at least three times after application. The interval can be determined according to the mode of action and duration of the pesticide. The first survey is conducted 1 to 2 days after application.
4.2.3 Calculation method of pesticide efficacy
The pesticide efficacy is calculated according to formula (1), formula (2) or formula (3): 371
GB/T 17980.7--2000
Number of mites before application - number of mites after application
Reduction rate of mite population (%)
Number of mites before application
Control effect (%)=
CK,× PT
CK,× PT.
Or control effect (%)
100 - CK
Where: PT. The number of mites before the treatment in the treatment area; PT, - the number of mites after the treatment in the treatment area;
- the number of mites before the treatment in the blank control area;
CK, the number of mites after the treatment in the blank control area;
PT - the rate of reduction of mites in the treatment area;
CK - the rate of reduction of mites in the blank control area. 4.3 Direct effects on crops
· (3)
Observe whether the pesticide has any damage to the crops and record the type and extent of the damage. In addition, the beneficial effects on the crops (such as accelerated maturity, increased vitality, etc.) should also be recorded.
Record the damage in the following ways:
a) If the damage can be counted or measured, it should be expressed in absolute values, such as plant height. b) In other cases, the extent and frequency of the damage can be estimated by the following two methods: 1) Record the damage in each plot according to the damage grading method, expressed as one, ten, ten ten, ten ten ten, ten ten ten ten. Grading method of pesticide damage:
One: No pesticide damage;
Ten: Mild pesticide damage, no impact on normal crop growth; Ten Ten: Moderate pesticide damage, recoverable, no crop yield reduction; Ten Ten Ten: Severe pesticide damage, affecting normal crop growth, causing a certain degree of loss in crop yield and quality; Ten Ten + Ten: Severe pesticide damage, crop growth is hindered, and crop yield and quality loss are serious. 2) Compare the pesticide treatment area with the blank control area to evaluate the percentage of pesticide damage. At the same time, accurately describe the pesticide damage symptoms of the crop (dwarfing, chlorosis, deformity, etc.). 4.4 Impact on other organisms
4.4.1 Impact on other pests and diseases
Any impact on other pests and diseases should be recorded, including beneficial and unbeneficial impacts. 4.4.2 Impact on other non-target organisms
Record any impact of the pesticide on wild organisms and beneficial insects. 4.5 Product quality and yield
Generally, no record is required.
5 Results
Use Duncan's new multiple range (DMRT) method to analyze the test data, and use corresponding biostatistics methods in special cases. Write a formal test report and analyze and evaluate the test results. The test report should list the original data. 372
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