NY/T 2500-2013 Guidelines for testing specificity, consistency and stability of new plant varieties Konjac NY/T2500-2013 |tt||Standard compression package decompression password: www.bzxz.net

1CS 65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T 2500—2013
Guidelines for the conduct of tests for distinctness, uniformity and stability-Konjac
(Amorphohallus )
Issued on 2013-12-13
Implemented on 2014-04-01
Issued by the Ministry of Agriculture of the People's Republic of China
Scope·
Normative reference documents
Technical spectrum and definitions
Requirements for breeding materials
Testing methods
Specificity,
Character table
Determination of consistency and stability results
Dividend characteristics
1 Technical questionnaire·
Appendix A (Normative Appendix)
Konjac characteristics
Appendix (Normative Appendix)
Interpretation of konjac characteristics
Appendix ((Normative Appendix))
Format of technical questionnaire for konjac
NY/T2500—2013
NY/T 2500—2013
This standard was drafted in accordance with the rules given in G/1.1
Technical Standards The Seed Administration Bureau of the Ministry of Agriculture and Rural Affairs proposed this standard. This standard was approved by the National Technical Committee for Standardization of New Varieties of Dry Products (SAC/T: 277). This standard was drafted by: Crop Research Institute of Sichuan Academy of Agricultural Sciences, Agricultural Science and Technology Development Center of Southwest University. The authors of this standard are: Liu Youli, Yu Yi, Zhang Shenglin, Zhang Jianfeng, Jiang Xuezhu, Xian Dengning, Lan Jingping, Jiao Rong, Huang Weizao, He Malin
1 Scope
NY/T 2500—2013
Guidelines for testing specificity, consistency and stability of new plant varieties
This standard defines the technical requirements and general principles for testing specificity, consistency and stability of new varieties. This standard uses the specificity, consistency and stability test results of new varieties of the genus Amorphiohattus Blutne to define 2 Normative references
The following documents are indispensable for the formulation of this document. For all dated references, only the dated version applies to this document. For all undated references, the latest version (including all amendments) applies to this document. G/119557.1 Guidelines for testing specificity, consistency and stability of new plant varieties General principles 3 Terms and definitions
The terms and definitions defined in GBT19557.1 and the following terms and definitions apply to this document 3.1
Single measurernent of a group of plants 3.2
measurement of a number of individual plants or parts of planis Individual measurement
Measurement of a number of individual plants or parts of planis Individual measurement
Measurement of a number of individual plants or parts of planis 3.3
Visual inspection by single olservation of a group of plants or parts of plants Visual inspection of a number of plants or parts of plants 3. 4
Individual visual assessmentrnent assessment of individual plants ar paris of planisMonthly assessment of a batch of plants or a certain organ or part of a plant to obtain a correct individual record: 4 Symbols
The following symbols apply to this document:
MG: Sample measurement;bzxz.net
MS: Individual measurement;
VG: Group measurement.
VS: Daily measurement.
QI: Quantitative trait;
Q: Quantitative trait.
PQ: Pseudo-quality: Growing condition
(a)(g) The content of the mark is explained in detail in 2 (+): The content of the mark is explained in detail in 3: No document 4 The underlined part specially indicates the applicable scope of the test trait NY/T 2500—2013
5 Requirements for propagation materials
5.1 The propagation materials shall be provided in the form of bulbs. 5.2 The number of bulbs submitted shall be no less than 50.
5.3 The bulbs submitted shall be healthy in appearance, vigorous, free of diseases and insects, and shall be two-year-old bulbs with a diameter of cm and uniform individual growth. 5.4 The bulbs submitted shall generally not be subjected to any treatment that affects the expression of the variety's traits. If they have been treated, detailed treatment information shall be provided. 5.5 The bulbs submitted shall comply with the relevant provisions of the country's plant quarantine. 6 Test methods||t t||6.1 Test period
The test period is at least one growth cycle. 6.2 Test location
The test is carried out at one location. If some traits cannot be fully expressed at this location, other locations that meet the conditions shall be selected for observation.
6.3 Field test
6.3.1 Test design
Uncommon varieties and similar varieties are planted adjacent to each other in a pit-planting manner. Each plot has at least 12 plants, with a stacking distance of 30 cm and a row spacing of 60 cm. There are 2 replicates in total. 6.3.2 Field management
can be carried out according to the current production management method. 6.4 Character observation
6.4.1 Observation period
Character observation should be carried out according to the growth stages listed in Table A.1 and Table A.2. The growth stages are described in Table B.1. 6.4.2 Observation method
Character observation should be carried out according to the measurement methods (VG, VS, MG, MS) specified in Table A.1 and Table A.2. The measurement methods of some characters are shown in B.2 and T3.3.
6.4.3 Number of observations
Unless otherwise specified, the number of samples for individual character measurement (VS, MS) should not be less than 1, and the number of samples for each plant in the organs and parts of the observed plants should be 1. The whole plot or a mixed sample of a specified size should be observed for group observation of characters (VG, M). 6.5 Additional tests
When necessary, additional tests may be performed on the traits listed in Table 2 or traits not listed in this document. 7. Determination of specificity, consistency and stability results 7.1 General principles
The determination of specificity, consistency and stability shall be carried out in accordance with the principles of (G3, 19557.1). 7.2 Determination of specificity
The proposed variety shall be clearly distinguished from all existing varieties. When the proposed variety has obvious and reproducible differences from similar varieties in individual traits during the test, the proposed variety can be determined as having specific characteristics. 7.3 Determination of consistency
The proposed variety shall be determined based on the group standard of 1 and the acceptance probability of at least 95%. When the sample size is 1, at most 2 specific types can be identified. 2
7.4 Determination of stability
NY/T 2500--2013
If a variety has consistent characteristics, it can be considered that the variety has stability. Generally, no qualitative selection test is carried out. If necessary, the next generation of the variety can be planted. Compared with the previous varieties, if there is no significant change in the characteristics, the variety can be determined to be stable.
8 Characteristic table
According to the test results, the traits are divided into basic traits and selected traits. The basic traits are the traits that must be used in the test. The basic traits are shown in Table A.1-Selected test traits. 8.1 Overview
The trait table lists the trait name, expression type, expression status and corresponding code and standard variety, observation period and method, etc. 8.2 Expression Types
According to the expression method of the trait, the trait is divided into three types: quality trait, pseudo quality trait and quantitative trait. 8.3 Expression Status and Corresponding Codes
8.3.1 Each trait is divided into a series of expression statuses to facilitate the definition of the trait and the specification description: Each expression status is assigned a corresponding digital code to facilitate data recording, processing and variety description establishment and communication. 8.3.2 For quality traits and pseudo quality traits, all expression statuses are listed in the test guide: For quantitative traits, in order to shorten the length of the trait list, the expression status of the even-numbered code is not listed. The expression status description of the even-numbered code is in the form of the previous expression status to the next expression status,
8.4 Standard varieties
Traits Table 4 lists "standard varieties for reference for the expression status of some traits to help" determine the different expression states of related traits and correct the differences caused by environmental factors:
9 Grouped traits
In wood character, the cultivar grouping traits are as follows
) Petiole: spots (Table A.1 Neutral trait 15)
) Night applied - Sichuan petiole spotted varieties: Petiole: spotted pattern (Table A,! Neutral trait 16) Only Sichuan petiole spotted varieties were used: Petiole: spot color (Table A.1 Neutral trait 17). )Petiole: bulbil at the bifurcation point (Table A.1 neutral characters 18))Leaf: shape of leaflets at the top (Table A.1 neutral characters 23))Ball: flesh color (Table A neutral characters)
10 Technical Questionnaire
Please fill in the Technical Questionnaire NY/T2500—2013 in the format given in the Appendix
A. 1 Basic properties of konjac
: Depth
Special: Height
Bud: Tip shape
Peel: Number
1 Moment: Color
Complex origin and location of the pattern:
Peel: Short pattern color
Phosphorus 4: Content
: Nature
Appendix A
【Normative Appendix】
Table of konjac properties
Table A.1 Table of basic properties of konjac
VG, MS
State
Near-quality
Green
Pink
Yellow pot color
Fruit color
Pink
Non-industry
Standard variety
Pingshan Magic City
Yuning No.
Pearl taro
Chishan Baimohua
Yumo"7||t t||Huanxi Qianyu
Jinfoshanban
Shandili
Yumo No. 1
Jinbi Ranjie
2009-103
Nongban No. 3
Yumo 17
Mountain Konjac
Jinfo 1 Wide!
Yumo·No.
Jinfoban
Magic No. 1
Yongshan Mo Production
Waste Treatment No. 1
;: posture
mesophyll; rough skin
+petiole: length
Na: surface limit filter
(1)
leaf pot: main color
leaf feather: spots
instrument suitable for fertilizer on leaves with obvious species; leaves
collar: secret case
only HF petiole has variety: plan
petiole: spots Color
Handle: Beads at fork
Leaflets: Quantity
Table A1 (grade)
Current time and method
Table inspection and collection
He Jing Ma You
Cimen color
Dark green
Brown yellow
Spiral mouth color
Brown green
Standard variety
NY/T 2500-2013
Dehong Zhujingcha
Nongbei No. 2
Chuanshan Pang
Qiarang No. 1
Nongmo!
Jiangshan Cuishou
Yudu" No.
2009-003
Haima No. 1
Youshu Zhaojie
Zhanbai Class
Dehong Huapengtang
Shuhuijingyu
Nongdong No. 3
Yongshan H Yu, etc.
Silting No.
Original Mountain White Devil
Hanque! Let
Laobu quality film
2000-103
Nipuao
Zhu Lexue
Drop condensation 1 gram
Agriculture and Forestry: No.
NY/T2500—2013
Leaflet: Yan position
: Level Jing Leaflet edge shape
Leaflet slippery: Glossiness
piece, generally end leaflet shape
Leaflet: Level end leaflet-light leaking shape 1
Ball leather: shape
Ball falling: Bud eye shape
Ball look: Flesh color
A.2 magic Taro selection test properties
See Table A.2.
Extract: Poly content
Seeds: Powder
Table A.1 (continued)
Testing and methods
Active state
Azure green
Charcoal green silk
Broad lanceolate
Lead to the world
Anti-energy shape
Burn type
Yellow door color
Table A.2 Konjac selection test properties table
On-time production and methods
Exercise state
Cup elegant product
Pearl bud beautiful festival
298 - 003
Yuhuo number
Husband and daughter's home production
20n3 -80
Yufei No. 1
Dehong Pearl Demon Festival
Sparse Hair Grinding
Guizhou No. 1
Wu Wen Magic Hu
Pearl Ya Kang Festival
Yulu No.
Pearl Tooth Demon Jie
Du Mo 15
Hai Mo Hua
Pearl Ya Amorphophallus
Sen Ya Pin Zhi
2016-39
Xi Zao City
Yu Lu: No.
Pearl Doctor
Inflorescence Habitat: Length
Bo Yan Bao: Shape
Buddha Bag: Long Banquet
Fei Yan Cha: Internal Test Main Color
Spadix: shape of base
Spadix: outer ring at base
Spike: position relative to spadix
Spike length
Fleshy spadix: hollow
Fleshy spadix: medium spadix
Fleshy spadix; Pheasant length
Table A.2 (continued)
Test period and method
Expression status
Light color
Light green
Brown
Inverted bell
Funnel
NY/T 2500-2013
Standard Varieties
Yuwei
Xilan Ningfu
Ximo Fu
Yumo:
Sulfur Hair Deer Fu
Yujun
Youyuci
Pingshan White Konjac
Pearl Yam Magic Bull
Sparse Hair Magic Ning
Yulu||tt| |Yu Mo"
2C11-001
Pingshan Concave Spot
Shu Lao Mozi
2010-091
Xi Luo Mo Festival
Yu Mo No. 1
Yu Yan【No.
Yu Lu No. 1
Pingshan Line School
Xi Yi Mo Fu
21::01
23_0 1
Wanmao Mo City
Xi Lian No.
NY/T 2500—2013
Succulent inflorescence: attached to the heart organ length
Even spike inflorescence: attached to the lip-shaped bag
Succulent inflorescence: attached to the ageing face bag
Succulent inflorescence: Chen male organ hair
Fruit position
Pearl: shape makeup
Table A.2 (continued)
Training period
Table transportation state
Light yellow
Pineapple purple
Equal color
Long round shape
Standard variety
Control gaze
Yu Zhao, No.
Suoshan Court
Shujing City
2516 -01
Yu Mi 7
Pingshan: Magic
Sparse·Mao Moshi
Sister Yamjac:
Flowing Hairy Hoof Festival
Zhu Mao Feng Festival
B.1 Konjac Growth Stage
Table B. 1.
Yin Gong Xiao Yang Growth Area
Kang E Xiu Low Period
Main Bud Bright Period
Ball Big Period
Yuan "Long-term
Extremely Mirror Dormant Thorn
Shi Long Period
Fruit Maturity Period
Interpretation of Multiple Characters
(Normative Appendix)
Interpretation of Konjac Character Table
Table B.1 Konjac Growth Stage The table
ball state is relieved from the throat, the main plant
planted body exposed mountain pond and
special piece is developed in. The height of the cultivation is not skin cultivation river
the leaves naturally lodging
surname out of the ground, the flower sequence
is the realization of the product system store city color Luo
all the characters related to the buds and scales are measured on the 5th day of the labor break, and
the right related to the river and the tree's character observation research a praise.The leaf color, disease pattern and other characteristics of the petiole are observed, (r)
all parts and the spathe are wrapped in attached code devices, etc. The plants are white and naturally flowering. All the characteristics involving the thickness of the leaves or pedicels are observed. The leaves are tightly packed at the ground ()
all the fruit-only characteristics are observed for 3 years or more. The plants naturally bear fruit and the flowers are natural ()
all the characteristics related to the bulb identification are observed. The bulbs fall naturally after harvest and the bulb identification is requested. B.3
Explanation of individual characters
Only shape: bud: thickness - bud top; d close to the ground and browse the characteristics of the body. Character 2 wear: height, the height change from the ground to the top of the bud. Character? Bud, tip shape. Tooth shape. No figure. 1 Avoid south shape
Figure H.1 Bud: tip shape
NY/T 25002013
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