This standard provides the main biological characteristics, growth and reproduction, genetic characteristics and detection methods of the bigmouthed bull mullet. This standard is applicable to the detection and identification of the germplasm of the bigmouthed bull mullet. SC 1064-2003 Bigmouthed bull mulletSC1064-2003 standard download decompression password: www.bzxz.net

ICS 67. 120. 30
Aquatic Industry Standard of the People's Republic of China
SC10642003
Bigmouth buffalo
Issued on July 30, 2003
Ministry of Agriculture of the People's Republic of China
Implemented on October 1, 2003
Chapter 4 and Chapter 6 of this standard are mandatory, and the rest are recommended. Appendices A and B of this standard are normative appendices. This standard was proposed by the Fisheries Bureau of the Ministry of Agriculture
This standard was approved by the National Technical Committee for Aquaculture of Aquatic Products Standardization Technical Committee, and the unit of this standard: Beiyang Fisheries Research Institute The main drafters of this standard: Wang Jiaxi, Hu Shaohua, Yin Jiao, Zhu Chun-·, Han Zhang, Zhang WeiSC 1064—2003
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Bigmouth mullet
SC1064—2003
This standard is intended for the main biological characteristics, growth and reproduction, genetic characteristics and detection methods of bigmouth mullet (Valencietnes).
This standard is used to test some of the bigmouth mullet. 2 Normative references
The following documents are used as references to this standard. For all references with past dates, all subsequent amendments (excluding errors) or revisions do not apply to this standard. However, the parties reached or agreed upon based on this standard are encouraged to consider whether the latest versions of these documents can be used. For all references without date, the latest version applies to this standard. GB/T1SEE4, 1. Inspection of cultured fish germplasm Part 1: Inspection rules GB/T18654.2 Inspection of cultured fish germplasm Part 2: Sampling method GB/T1B64.3 Inspection of cultured fish germplasm Part 7: Determination of traits GB/T18654.12 Inspection of cultured fish germplasm Part 2: Analysis of color type SC1037-2000
3 Name and classification
3.1 Scientific name
Population buffalo fish [Icziour'pnelny (Valeniemn), also known as big-mouthed buffalo fish. 3.2 Common name
China's big-mouthed buffalo fish,
3.3 Classification position
Development (Cyriaifomes subfamily (Caroatomidae buffalo genus cuobis) 4 Main morphological characteristics
4.1 External morphological characteristics
4. 1. 1 Appearance
The body is spindle-shaped. The flesh is relatively hard, without teeth, the mouth is terminal, the anus is relatively large, especially the teeth, the body is relatively bright, the mouth is relatively large, and there is a row of reflective scales on each side of the body. The lateral line is median, straight and complete: the head is bright green, the upper part of the lower body is green, the lower part is dark blue, the upper body is white without ventral integument. The pores are relatively large and the ventral pores are not well developed. The quality is poor. The dorsal fossa is relatively long, with a V-shaped mouth depression, which is roughly triangular, and the shape is symmetrical.
The external morphology of the bigmouthed bullfish is shown in Figure 1. 4.1.2 Appearance
4.1.2.1 Dorsal shape
The dorsal fossa is slightly T3, iF-23-37.
4.1.2.2 The wax braid formula is A,ti-9,
4.1.2.3 The fiber length is 1064-2003
The formula is 37
4. t. 2. 4 The outer diameter of the first smoke bow is usually -80
4. 1. 3 Measurable traits
Figure The outer circumference of a large-mouthed bullfish is 2.6~3.8 times the body length: 35~4.4 times the head length, 3.9--13.9 times the tail length; the tail height is 1.1~-1.8 times the length of the hind shank 120~1.
4.2 Internal structural features
It has four standard tubular objects, which are connected by feather micro-tubes. 4.2.2 Dorsal vertebrae
Total number of vertebrae: 32~34
5 Growth and reproduction
5.1 Fish species in the Yangtze River Basin and the areas to its south Table 1 Weight of fish of each age group
Medium/fragile
Body weight/k
Body length/eu
5.2 Vegetative reproduction
5.2.1 Sexual maturity Age
55/8~631
x6. 8~-25. 4
Male fish is 3+ years old, female fish is 2+ years old. 5.2.2 Spawning type The sex of the individual is determined by the year-round spawning pattern. 5.2.3 Grain yield The relative fertility of the 4-year-olds is 25 × 1U/g body weight, 2 × 2 × 1 #74 × 29. 4 -.,, 6 × 2 × 1: 77n~·3 7n4 × 2 × 1: 43 ~-51 × 2 × 1: 6 ... 6.2 Biochemical and metabolic characteristics Figure 2 Lysogeny of the bigmouthed fish 6.2.1 The heart histology has a single enzyme band of LDH isozyme. Its LDH isozyme spectrum is shown in Figure 3. The tracer diagram is shown in Figure 4. |3 Electrophoresis spectrum of LDH isoforms in cardiac tissue
Figure 4 Scanning bands of LDH isoforms in cardiac tissue
SC10642003
8.2.2 Cardiac maintenance department: Activity intensity of LDH isoforms bands Table 2. Table 2 Activity intensity of LDH isoforms bands in cardiac tissue
15 Standard
7 Detection method
7.1 Sample
According to the definition of GB/T18654.2.
7.2 Determination of properties
According to the provisions of GB/T18654.3
7.3 Age determination
According to the provisions of SC137UI:
7.4 Lysinophil detection
According to the current provisions of GB/T18651.12
7.5 Simultaneous sea detection
7.5.1 Sample extraction
Suck the big mouth of the cow kidney fish such as the tail. Remove enzymes and collect blood at the same time, then remove the spines and quickly remove The heart tissue was washed with dehydrated water for 1 month, then transferred to a homogenized slurry in a 2:5 (highest quality) grade H8.9 buffer solution and cooled in a balloon bath for 4 h. The slurry was placed in a refrigerator for 4 h and centrifuged at 100 cm/s for 70 min. After centrifugation, the slurry was placed in a 50% low temperature box for isozyme electrophoresis analysis. 7.5.2 Polyacrylamide gel electrophoresis || tt || Separate the LDH with a high-temperature gel The electrophoresis is 26TA. The source of the electrophoresis is 4CA7.5.3. Spectrum analysis
Place the cloth (with brush) in the dyeing machine. After dyeing, wash it with ionized water 2 to 8 times. Fix the tape with cold acetic acid and 10% acetic acid. 0.00100: The isoenzyme gel section was scanned with a wavelength change to determine the isoenzyme bands. The distribution of various fiber bands and the isoenzyme bands were determined. 7.6 Test results were determined in accordance with the provisions of GH/T1HF54.1. 4.1 Electrode blasting mother stream Appendix A (Normative Appendix) Preparation of blasting solution SC 1064—2003 Add 1.628 mg of sodium sulfoxide to 1.31% of distilled water, add Tris(III) solution at a concentration of 8.00 g, and dilute to 2.5 L. Dilute with 1.1% sodium sulfate before use. .2
2 Sample buffer
Prepare 19% dilute ris ammonia magnetic buffer stock solution
(Normative Appendix)
L,DH Preparation of re-staining solution
H,1A Note
NBT
Coenzyme (NA5%
Methyl ester (MS) 20mg
Sodium chloride 290mg
Heat to 1C5L
1m0l/L Sodium lactate
Sodium lactate Set
Add distilled water to
o,Iris hydrochloric acid yH.1
Tris: ec.57 R
10]/. Plate acid to adjust pH to 7.1
Add garlic water 109m
Prepare color solution 5 in the proportion of 4 concentrate, 2 parts of solution B, 3 parts of solution, and 11 parts of distilled water
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