This part specifies the methods and requirements for field efficacy plot tests of fungicides for the prevention and control of apple tree rot (Cytosporamandshurica) and pear tree rot (Cytosporaambiens) lesion recurrence. This part applies to the registration efficacy plot test and efficacy evaluation of fungicides for the prevention and control of apple tree rot and pear tree rot recurrence. Other field efficacy tests shall refer to this part. GB/T 17980.116-2004 Guidelines for field efficacy tests of pesticides (II) Part 116: Efficacy test of fungicides for the prevention and control of apple and pear tree rot lesion (spot) recurrence GB/T17980.116-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and requirements for field efficacy plot tests of fungicides for the prevention and control of apple tree rot (Cytosporamandshurica) and pear tree rot (Cytosporaambiens) lesion recurrence. This part is applicable to the efficacy plot test and efficacy evaluation of fungicides for the prevention and control of apple rot and pear rot recurrence. Its field efficacy test is also carried out in accordance with this part.

ICS 65.100
National Standards of the People's Republic of ChinabZxz.net
GB/T17980.116--2004
Guidelines for the field efficaty trials ( I )-Part 116:Fungicirles against recur cunker uf apple and
Pesticide
Guidelines for the field efficaty trials ( I )-Part 116:Fungicirles against recur cunker uf apple and
pear2004-03-03 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration
2004-08-01 Implementation
GB/T17880.116-2094
It is one of the important contents of pesticide registration management and is the important technical basis for formulating pesticide product standards. The standard is the only guide for the safe and reasonable use of pesticides. In order to standardize the methods and contents of pesticide daytime testing, make the test more scientific and unified, and align with international standards In order to make my country's two-effect test report have international recognition, my country has formulated national standards for efficacy test. This series of standards is based on my country's actual situation and is formulated after large-scale field efficacy tests: Apple and pear rot are the main diseases of apple trees and pears in my country, and sterilization is often required for prevention and control in production. In order to determine the dosage of the agent for treating the recurrence of fruit and pear rot, the test agent has no effect on the fruit. The effect of the dosage form on beneficial organisms is to provide a basis for the efficacy and safety of fungicides registered for use. This part of B/T17980 is specially formulated: This part is one of the standards for field efficacy tests of pesticides (II). This part is an independent part proposed by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this part is: Pesticide Control Institute of the Ministry of Agriculture. The main contributors to this part are: Yuan Baoliang, Tu Jindu, Wu Xinshi, Zhi Naishuang Liu Pang, Shi Yin, Shang Zengqiang. This part is interpreted by the Pesticide Control Institute of the Ministry of Agriculture. 274
1 Scope
Field pesticide Part 116: Effect test standards (II) of fungicides against apple and sharica rot scars (spots) GB/T:17980.116-2D04 This part defines the methods and requirements for field efficacy plot tests of fungicides against short-term symptoms of Cytopora and Sharica rot (Ctospora ruft), and the field efficacy plot tests and efficacy evaluation of fungicides against recurrence of fruit tree rot and pear pain rot. The test conditions shall be carried out in accordance with this part. 2.1 Selection of crop varieties and test subjects The test subjects are Cytopora and Sharica rot. The test crops are apple trees and trees. Select sensitive fruit tree varieties, record tree age and tree variety name. 2.2 Environmental conditions
The test should select mature fruit trees with more serious disease and better tree vigor in previous years to ensure that there is a certain safety range for the disease after the treatment without applying the medicine. All test plots (soil type, fertilizer, variety, tree age, row spacing, etc.) should be consistent and in line with the local scientific agricultural practice (GAP).
3.1 Test agent
3.1.1 Test agent
Indicate the trade name or code of the agent, general name, product name, dosage form, content and manufacturer. The test agent treatment should be no less than three times or the amount of medicine specified in the agreement (the test agreement designed by the test committee and the test undertaker). 3.1.2 The control agent should be a registered product that has been proven to have good efficacy in practice. The type and action formula of the control agent should be similar to that of the test agent and the commonly used local measurement should be used. 3.2 Plot arrangement
3.2.1 Plots should be arranged when
the peak period of spring rot occurs. According to the test requirements, a certain number of the most rotten diseased plots (newly converted and recurrent diseased plots) that year should be selected + scraped and numbered according to the routine method, and the location of the numbered lesions (rows of trees, plant guides and the parts of the trees where the lesions will occur) should be recorded. The diseased areas in the test plots should be arranged in sequence, and the plots should be arranged randomly.
3.2.2 Plot area and repetition
Plot area: each plot should have no fewer lesions (lesions), and the number of repetitions should be increased every year.
3.3 Application method
3.3.1 Usage method
Follow the agreement and the instructions for application. The application should be adapted to the local scientific agricultural practice. Generally, the lesions should be applied to the scraped or marked areas. 37
GB/T17980.116—2004
After the wound is fully brushed with the medicine roll: In addition to the wound scar (old wound scar), the bark around the wound is also fully coated with the medicine roll
3.3.2 Type of connection
Select the commonly used equipment in production and record the type of equipment used. Usually, a brush is used, and the width of the brush is generally about 3cm\-4 to ensure the adequacy of the medicine and the flexibility during operation. 3.3.3 Time and frequency of application
Follow the requirements of the agreement and the instructions on the label. Usually, the medicine is applied once after the wound is cured. If you want to know the actual situation of the disease and the duration of the drug, you can apply the medicine again one week or two months after the application, and record the number of applications and the duration of the drug. 3.3. 4. Use the dosage and amount of the drug according to the requirements of the agreement and the dosage indicated on the label. The content of the active ingredient in the drug test is expressed as mB/., and the number of drugs used is required to be recorded. 3.3.5. Data requirements for the control of other pests and diseases If other drugs are used, drugs that have no effect on the test acid drugs and the test objects should be selected, and all test plots should be uniformly tested for a period of time. They should be used separately from the test drugs and the control drugs to minimize the interference of these drugs. The difficulty of using such drugs should be recorded. 4 Investigation, recording and measurement methods 4.1 Meteorological data and soil density data 4.1.1 Meteorological data During the test, the data of rainfall (daily rainfall, including daily average temperature, maximum and minimum temperature changes, including no change) and humidity (daily average temperature, including daily average temperature, including daily average temperature, including daily average temperature, including daily average temperature, and daily average temperature) should be obtained from the test site or the nearest meteorological station. During the whole test period, the adverse climatic factors that affect the test results, such as long-term dry season, windstorm, ice sheet, fall, etc., should be recorded. 4.1.2 Soil density data Record the soil type, topography, soil fertility, sugarcane water content, and other information. 4.2 Survey methods, time and details
4.2.1 Survey methods
Survey the recurrence rate of the test scars (blocks) in each plot, and measure the average thickness of the healing tissue of the first three scars in the plot, expressed in mm. When the test scars (blocks) need to be removed during winter pruning of fruit trees due to disease, it should be done after the full effect has subsided.
4.2.2 Survey time and details
Carry out according to the requirements of the proposal. Usually, the lesions treated with drugs in the late autumn and early autumn should be investigated every 15 days after the drug application, during the outbreak period of the disease in the late autumn and early autumn. The last investigation should measure the spread of the lesions. The lesions treated with drugs in the autumn and winter should be investigated every 15 days after the drug application, during the outbreak period of the disease in the early autumn and early autumn. The last investigation should measure the spread of the lesions. 4.2.3 Calculation method of drug efficacy
Symptoms (spread) Recurrence (%) - End (spot) Total number of lesions × "0) Total number of lesions (spots)
.......( 1
Prevention and control effect (%) - Empty control area lesions (spots) Drug type treatment area cancer spots (recurrence) × 100 (2) Indoor control area lesions (spots)
Symptoms (spots) Average spread (cured) _2 [Each lesion intensity (mm)] ×200-（ ）Measurement disease (spot) total block fee
Promote disease protection (spot>micro-combination effect (%>-room self-to-original area disease sore () flat potential one drug support area bottom cancer "yuan) some average combination rate ×10 empty mountain control area disease range (spot) average interest combination rate..(4)
1.3 Other effects on crops
: Observe whether the crop needs to produce drugs, if there are drugs, record the drug process and type, 87E
Record according to the following method:
GR/T17980.116—2004
In the bactericidal efficacy test for preventing and controlling the recurrence of old rot scars on apple and pear trees, the drug damage that occurred is generally manifested as the damage of the drug to the bark of the diseased scar (spot), causing the tissue to turn black and necrotic, among which the upper and lower parts of the scar are taken as the vertical part: the degree of damage can be expressed by a numerical value. The specific statistical method is as follows:
Apply the drug to the outside and select the scar in the empty map (the first 3 areas before the decision), measure the length of the upper and lower ends of the scar (expressed in square meters), and calculate the drug concentration after heating.
Degree of drug damage (road)
The total length of the diseased tissue of each plot in the drug treatment area is the total width of the diseased tissue of each plot in the drug treatment area × 1G0 (5) The total length of the dead tissue of each plot in the control area is the total length of the dead tissue of the control area. When the upper and lower ends of the diseased area are formed, the formula uses \+"When the dead group is found, the formula uses \-"4.4 Effects on other organisms
4.4.1 Effects on other pests and diseases
Record any effects on the test area, including positive or negative effects. 4.4.2 Effects on other non-target organisms
Record the effects of the drug on wild organisms and insects in the test area. 4.5 Product yield and quality
Not required.
5 The results obtained from the experiment must be analyzed using statistical methods (using the MR method). The conclusions should be written in a formal way and the test results should be analyzed. The original data should be kept for inspection and verification.
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