This standard specifies the artificial insemination technical links of pig semen collection, semen quality inspection, dilution, packaging, storage and transportation, insemination, etc. This standard applies to artificial insemination operations of pigs. NY/T 636-2002 Technical Regulations for Artificial Insemination of Pigs NY/T636-2002 Standard download decompression password: www.bzxz.net

ICS 65.020
Agricultural Industry Standard of the People's Republic of China
NY/T636—2002
Rules for swine artificial insemination techniques2002-12-30 Issued
2003-03-01 Implementation
Promulgated by the Ministry of Agriculture of the People's Republic of China
Appendix A of this standard is a normative appendix, and the appendixes are informative. The standard is issued by the Ministry of Agriculture:
NY/1636—2002
The main contributors of this standard are: National General Station of Animal Husbandry and Veterinary Medicine, Quality Supervision and Inspection Center of the Ministry of Agriculture (Guangzhou), South China Agricultural University, Tangxia Hunting Ground of Dongguan Food Import and Export Corporation, Guangdong Province. The main contributors of this standard are: Liu Hailiang, Ling Qiuxiang, Zhang Shouhui, Zhang Guoji, Zhang Xiaohong, Zhang Daijian, Xue Ming. 1 Scope
Technical Regulations for Artificial Insemination of Pigs
NY/T636-2002
This standard specifies the technical links of artificial insemination such as quality inspection of semen-sugar solution, barrel creaming, packaging, storage and transportation of semen, and is applicable to artificial insemination of pigs. 2 Semen Collection
2.1 Male Training Age
2.1.1 Training Age: Male pigs aged 7 to 8 months can also be trained after mating. 2.1.2. Methods of collecting semen: The semen of adult boars must be recorded in the hindquarters of the boar. The boar can be trained to mount slowly. It can also be trained by using estrus sows to attract the boar. When the boar is in the mood, the boar can be quickly separated from the sow and trained to mount the sow. This can be done once or twice a day, and the training time should not exceed 15 inches each time. 2.2 Methods of collecting semen: 2.2.1 Preparation before collecting semen: 2.2.1.1 Preparation of boar for collecting semen: Cut off the long hair on the foreskin of the boar, rinse the surface of the boar with clean water. 2.2.1.2 Preparation of semen collection equipment: The semen collection equipment should be kept in a constant temperature sticky container and prepared with paper and gauze to clean the dirt in the foreskin of the boar and clean the gauze of the sow.
2.2.1.3 Preparation of semen diluent: prepare the desired semen diluent, place it in water and heat it to 35°C. 2.2.1.4 Preparation of semen quality inspection equipment: adjust the microscope stage, constant temperature plate and heat density meter for quality inspection.
2.2.1.5 Preparation of semen packaging equipment: semen packaging device, semen storage bottle or bag, etc. 2.2. 2 Semen collection procedure
2.2.2.1 Disinfect the boar's foreskin with 0.7% highly sensitive acid solution, and then clean it with warm water: 2.2.2.2 The collector holds a 37℃ semen cup (with a disposable food bag inside and covered with 2 to 3 layers of gauze) in one hand and uses a double-layer latex hand in the other hand to squeeze out the boar's foreskin, press the boar's foreskin, stimulate the boar's foreskin to slightly crawl out and expel the penis, take off the original gloves, hold the extended boar's penis head tightly with the hand, and push the "S" shaped penis along its direction of impact. When the boar ejaculates, collect the semen in portions or in full in the semen collection box. The small amount of semen (mL white) ejected by the boar is not collected until the boar ejaculates.
2.3 Semen collection frequency
Semen collection frequency is determined by the number of effective sperm obtained per unit time: to achieve fixed point, time, and person. Adult boars should come to the semen collection no more than 2 to 3 times a week, and young boars should come to the semen collection once or twice a week. 3
Semen injection inspection
3. 1 Semen collection volume
Weigh the semen after collection.
3.2 Color
Normal semen should be milky white or grayish white. Semen with abnormal colors such as yellow, yellow, red, and reddish red should be discarded. 3.3 Odor
NY/T636—2002
Pig semen should have an odor. If there is a strange odor, it should be discarded. 3.4 PH
Measure with pH or pH test paper, normal range is 7.0~7.8 3.5 Sperm motility
The percentage of sperm with vertical motion in each case, estimated on a scale of (1~1.0, the requirement of motility is not less than 0. The slide or cover slide should be preheated at 37°C. 3.6 Sperm density
Use cell counting plate or semen analyzer to measure the sperm density per liter. 3.7 Sperm deformity rate
Observe the sperm deformity rate with ordinary microscope or related microscope, the deformity rate should not exceed 18%. Check the deformity rate of each boar every two weeks.
3.8 Fill in the boar semen quality inspection registration form (with matching 4.1.1 Dilution formula of semen preparation 4.1.2 Measure the amount of light powder according to 10ccml, 2000ml, and add it to the package. 4.1.3 Use 1h semen or the prepared dilute sample to add a certain amount of distilled water, and use a stirrer to help it dissolve. 4.1.4 Use 0.11mol/L diluent or 0.1mol/L sodium hydroxide to adjust the pH of the dilute solution to about 7.2 (6.B--7.4). After the diluent is prepared, it should be labeled in time, indicating the product name, preparation date and time, etc. The prepared dilute solution can be used for the preparation of the preparation solution after 1h. .5 The dilution should be dried in a 4°C incubator. It should not be stored for more than 20 minutes. 4.2 Semen dilution 4.2.1 After the semen is collected, it should be diluted as soon as possible. The original storage should not exceed 20 minutes. 4.2.2 The temperature difference between the dilution and the sieve should not exceed 1, that is, the dilution liquid should be heated to 330~37, and the humidity should be measured by the semen temperature. The reverse operation cannot be performed. 4.2.3 When diluting, add the dilution liquid to the semen, then gently stir or use sterilized water to stir it to make it uniform. 4.2. When high-magnification sieving is performed, low-magnification sieving (1:1)~(1:2) should be performed first. After 0.5 minutes, the remaining sieve solution should be slowly added along the wall. ||4.2.5 Determination of the estimated number of sieves: Determine the screening multiple according to the output volume of 89mL--100mL, including more than 3 billion effective cases. After dilution, the sperm must be tested for viability at about 5% and then the sperm must be checked for viability at more than 0.6 for packaging and storage. 4.2.6
4.2.7 Mix the semen, and then dilute the fresh and tough concentrates of the first boar at a ratio of 1:1. According to the sperm density and sperm count, the dilution multiples should be calculated and the whole concentrate should be added. Then, evenly disperse. 4.3 Semen packaging
4.3.1 Adjust the semen packaging machine, and pack the semen into new bottles or cups in units of m.--100ml. 4.3.2 The boar breed, number, production period, shelf life, tax reduction name and production unit, etc. should be marked on the bottles or cups. 4 Accurate storage
4.4.1 After the semen is placed at 2:1 for 1h~1, it is stored in a 17℃ constant temperature box. You can also put the sick liquid bottle or the package wrapped with a towel directly into a 17℃ constant temperature box.
4.4.2 High-effective dilution can be stored for 3 days. Medium-acting dilution can be stored for 4d~5, and long-acting dilution can be stored for 7d~9. No filter can be used to store semen until it is completely used up.
4.4.3 Gently turn it over every 125 to prevent precipitation and death. 4.5 Transportation of semen
NY/T636—2002 Www.bzxZ.net
Semen should be placed in a well-protected container for transportation. Avoid strong shaking during transportation. 5.1 Insemination time: 8h~12b after the estrus sows have standing reflex, the first insemination is performed: 8h~13b after the second and third inseminations are performed: 5.2 Semen inspection: Take out the accumulated fluid from the machine, gently remove it, use a dropper that has been condensed, and place it on a preheated plate at 37°C for a while, check the activity under a microscope, and use it for 6 hours before use. 5.3 Insemination: Do not use the sterilized or disinfected sperm tube for insemination. 5.4 Procedure for transfusion
5.4.1 Disinfect the hands of the sperm collector:
5.4.2 Wet the hair along the vulva, vagina and surrounding area of ​​the sperm collector with water and probe the vulva.5.4.3 Take out the sterilized sperm tube from the sealed bag and apply lubricant on it.5.4.4 Insert the sperm tube into the pig's vagina at 45 degrees. When you feel resistance, slowly rotate clockwise and move your hands backward to feel the vas deferens and confirm the transfusion site.5.4.5 Take the sperm of qualified quality from the sperm storage foil and confirm the breed and ear number of the male.5.4.6: Turn the sperm tube upside down and cut the bottle cap (or tear open the mouth) with scissors to ensure that the sperm can flow out.
5.4.7 Adjust the insemination time by controlling the height of the insemination bottle (box) and the intensity of stimulation to the sow. The insemination time requirement is 3rmim.~in.
5.4.8 When the semen in the insemination bottle (box) is emptied, lower the semen bottle (box) for about 15 minutes, and observe the liquid flowing back to the insemination bottle (box). If there is backflow, re-inject it.
5,4.9 In the case of preventing air from entering the sow's reproductive tract, the insemination tube should be retained for 5 minutes! 5.4.10 Record the replacement of the extraction record (see Appendix (). 3
NI/T636-2002
Appendix A
(Normative Appendix)
Public liquid quality inspection record
Public quality inspection record
Deformation
Rate!
Behind the scenes
Pre-measurement/
Storage time
D-box
Lemon micro-stock three-step
EDTA fish
Potassium
Blue calculation
Glycolic acid chain toxin
Tris(Eri)
Cysteine
Lin Institute
【Spotted material attachment】
See several male essences diluted into formula
Decline day. 1
Common formulas for several male cat sperm diluents
Hospital 1
Formula 1
Appendix C
(Informative operation record)
Maternal sperm record table
Table C. 1Maternal sperm insemination record
1st insemination
Ear number time
Dye 2 Change of sperm
Time anti-risk
Calculation\change of sugar god
NYT6362002
Unit:/1x:ml.
Anti-seat flow
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