This standard specifies the environmental conditions, artificial propagation, seedling cultivation, grow-out techniques and disease control techniques for pollution-free aquaculture of turbot (Scophthalmus maximus Linnaeus). This standard applies to pollution-free aquaculture of turbot. NY/T 5153-2002 Technical Specification for Aquaculture of Pollution-Free Turbot NY/T5153-2002 Standard download decompression password: www.bzxz.net

ICS65.150
Agricultural Industry Standard of the People's Republic of China
NY/T5153—2002
Pollution-free Food
2002-07-25 Issued
Technical Specification for Turbot Farming
2002-09-01 Implementation
Issued by the Ministry of Agriculture of the People's Republic of China
NY/T5153--2002
This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the National Technical Committee for Aquatic Products Standardization.
The drafting units of this standard are: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Fisheries Technology Extension Station, Yantai Fisheries Technology Extension Center Fish Farm, Laizhou Ocean and Fisheries Bureau, Shandong Province. The main drafters of this standard are: Yu Dongxiang, Lei Qilin, Chen Siqing, Liu Xuezhou, Li Lujing, Wang Chunsheng, Zhao Yushan, Mu Junshan, Ren Yongchao. 186
1 Scope
Technical Specifications for Pollution-free Turbot Farming NY/T5153--2002
This standard specifies the environmental conditions, artificial propagation, seedling cultivation, grow-out techniques and disease control techniques for pollution-free farming of turbot (ScophthalmusmarimusLinnaeus). This standard applies to pollution-free farming of turbot. 2 Normative References
The clauses in the following documents become clauses of this standard through reference in this standard. For dated referenced documents, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For undated referenced documents, the latest versions shall apply to this standard. GB11607 Fishery Water Quality Standards
NY5052 Pollution-free Food Water Quality for Seawater Aquaculture NY5071 Pollution-free Food Guidelines for the Use of Fishery Drugs NY5072 Safety Limits of Pollution-free Food Formulated Feeds 3 Environmental Conditions
3.1 Site Selection
The farm should be located far away from pollution sources, with convenient communications and transportation, sufficient electricity, and a freshwater source. 3.2 Water Quality Conditions
The water quality of the water source should meet the requirements of GB11607. The water quality of aquaculture water should meet the requirements of NY5052. 4 Artificial Propagation
4.1 Facilities
There should be temperature control facilities, aeration facilities, light control facilities, water inlet and outlet facilities, and water treatment facilities. 4.2 Broodstock Selection
The appearance characteristics of the broodstock should conform to the description of fish taxonomy, with complete body shape, normal color, health, strong vitality, good feeding, fish age of more than 2 years old, and weight greater than 2kg.
4.3 Broodstock cultivation
4.3.1 Cultivation conditions
Stocking density is less than 5kg/m2.
Light intensity is 60Ix～4001x, and the light should be uniform and soft. Water temperature is 10C～14℃, salinity is 28～~32, pH is 7.8~8.2, and dissolved oxygen is maintained above 5mg/L. Flowing water cultivation, the daily flow is 4 to 6 times of the cultivation water body, continuous aeration and oxygenation, and timely removal of residual bait and dirt. 4.3.2 Feed feeding
Feed includes soft pellet feed and feed fish. The main components of soft pellet feed are powdered compound feed, feed fish, etc., and feed fish are mainly fresh and frozen small fish.
Compound feed should meet the requirements of NY5072. Feed should be of suitable size. The daily feeding amount of compound feed is 1% to 2% of the fish body weight, and the daily feeding amount of simple feed fish is 1.5% to 3.0% of the fish body weight. Feed once or twice a day. 187
NY/T 5153 ---2002
4.4 Fertilization and Hatching
4.4.1 Broodstock Sex Ratio
The male-female ratio of mature broodstock is 11 to 3.
4.4.2 Fertilization
Artificial insemination is performed by artificially squeezing out mature eggs and semen, and using dry or wet methods. 4.4.3 Egg Washing
Immerse the fertilized eggs in 15×10' iodine solution for 3min to 5min, and then rinse with clean seawater. 4.4.4 Hatching density
Put the disinfected and cleaned fertilized eggs into clean seawater and let them stand for more than 10 minutes. Select the floating eggs and place them in the prepared hatching pool. The hatching density of fertilized eggs is 1×10″grains/m2~2×10″grains/m2. 4.4.5 Hatching conditions
Light intensity 2001x~20001x, temperature 13C~15C, pH7.8~8.2, salinity 28~35, dissolved oxygen content greater than 6mg/L, and slightly aerate to make the fertilized eggs evenly distributed in the water. 5 Seedling cultivation
5.1 Cultivation facilities and conditions
5.1.1 Hatchery facilities
Should include seedling cultivation room, biological feed cultivation room, aeration facilities, temperature control facilities, light control facilities, water treatment facilities, and water inlet and outlet facilities.
5.1.2 Cultivation conditions
Light intensity 5001x～20001x, light should be uniform and soft. Water temperature 14C～18C, pH7.8~8.2, salinity 28～32, dissolved oxygen content 6mg/L or more. 5.2 Cultivation density
Newly hatched fry 1×10° tail/m2～2×10 tail/m2; metamorphosed bottom fry 1000 tail/m2～2000 tail/m. 5.3 Feeding
Seedling feed mainly includes biological feeds such as Chlorella, rotifers, Artemia and micro-granular compound feeds. The safety and sanitation indicators of compound feeds should meet the requirements of NY5072; Chlorella used during seedling cultivation should be fresh and pollution-free, and rotifers and Artemia should be rinsed clean and free of pathogens. Feed rotifers and Artemia 2 to 4 times a day. Each time rotifers are fed, the water content should reach 5/ml~~10/ml, and each time Artemia is fed, the water content should reach 1/mL~2/mL. The daily feeding amount of compound feed is 5%~15% of the fish body weight. The feed particles should be of suitable size and fed in a timely manner. It is advisable to feed less and more frequently. 5.4 Seedling quality and specifications
5.4.1 Seedling quality
Requires normal color, healthy without damage, disease, deformity, or albinism, strong activity, and good feeding. The full-length qualified rate and disability rate should meet the requirements of Table 1.
5.4.2 Seedling specificationsbzxZ.net
Full-length qualified rate
Disability rate
Should meet the requirements of Table 2.
Table 1 Requirements for the qualified rate and disability rate of seedlings in full length
5.4.3 Seedlings out of the pond
Seedling specifications
Small seedlings
Large strains
Specifications of turbot seedlings
Total length/cm
NY/T5153-2002
Seedlings are usually collected out of the pond by drainage, and seedling damage should be avoided during operation. Seedlings should be tested for quality and specifications when they are out of the pond. 5.5 Seedling transportation
5.5.1 The transportation methods include box-type or barrel-type container inflatable transportation and live water boat transportation, which can be selected according to specific conditions. 5.5.2 The water temperature and salinity of the transportation water can be adjusted in advance according to the requirements of the culture water ring magic infant. 5.5.3 Seedlings should be stopped from feeding for more than 1 day before transportation. 6 Cultivation
6.1 Cultivation method
Indoor cultivation in factories.
6.2 Cultivation facilities
Should include cultivation ponds, feed processing rooms, analytical laboratories, and aeration, temperature control, light control, water inlet and outlet, and water treatment facilities. 6.3 Cultivation conditions
Light intensity 5001x~~20001x, the light should be uniform and harmonious. Water temperature 10C~24℃, pH7.6~8.6, salinity 15~32, dissolved oxygen greater than 5mg/L. 6.4 Fish stocking
6.4.1 Pond entry conditions
The temperature difference between the water shock and transportation of the seedlings entering the pond should be within 2C, and the salinity difference should be within 5. 6.4.2 Stocking density
The stocking density should be adjusted according to the growth of turbot. The stocking density is shown in Table 3. Table 3 Stocking density of turbot during grow-out stage
Average total length/cm
6.5 Feeding management
6.5.1 Water management
Average weight/g
Stocking density (tail/m2)
200~300
100~150
The depth of grow-out water is generally controlled at 60cm~80cm, and the flow rate is 5~10 times that of the grow-out water body, and is adjusted according to the grow-out density and water supply conditions. The grow-out water body should be clean and pollution-free, and the dirt in the pond should be removed in time. 6.5.2 Feed
6.5.2.1 Types
The feed for grow-out includes soft pellet feed and feed fish. Soft pellet feed is made by mixing powdered compound feed with feed fish: feed fish can be fed directly after washing, but it is not suitable to feed a single species of feed fish for a long time.
6.5.2.2 Safety requirements
The safety and sanitation indicators of compound feeds shall comply with the provisions of NY5072; feed fish shall be fresh, disease-free and pollution-free. 6.5.2.3 Feeding management
The daily feeding amount of compound feeds shall be 1% to 2% of the fish body weight, and the daily feeding amount of feed fish shall be 1.5% to 3% of the fish body weight. The specific feeding amount shall be determined according to the fish feeding situation, and no residual feed shall be allowed. For fish weighing less than 200g, feed 3 to 4 times a day; for fish weighing 200g to 300g, feed 2 to 3 times a day; for fish weighing 300g to 400g, feed 2 times a day. When the water temperature is below 12C or above 22C and the fish are not feeding well, the feeding frequency and feeding amount shall be appropriately reduced. 7 Disease prevention and control and drug use
7.1 Observation and testing
Observe the feeding, swimming and growth and development of fish at different stages with the naked eye regularly, find sick and dead fish in time, remove sick and dead fish for dissection and analysis, observe under a microscope, and analyze the cause. Deep burial should be done for sick and dead fish. 7.2 Prevention and control principles
Prevention should be the priority, and comprehensive measures such as light control, temperature adjustment, water quality treatment, and increased water flow should be adopted. 7.3 Drug use
Drug use should comply with the requirements of NY5071; the use of microecological preparations and immune preparations for disease prevention is recommended. 190
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