Some standard content:
Appendix A and Appendix B of this standard are normative appendices. This standard was proposed and approved by the Ministry of Industry and Information Technology of the People's Republic of China. The drafter of this standard is: China Agricultural University. The drafter of this standard is: Chen Liguo, Zhu Er, GB19169-2003
CB 19159-2003
The chrysanthemum varieties used in the cultivation of chrysanthemum are a mixture of artificially cultivated pure chrysanthemums and their culture media. my country adopts a three-level expanded breeding procedure (i.e. mother seed, original seed, and descendant seed) to cultivate chrysanthemum varieties. In order to standardize the production, distribution and use of this variety in my country and ensure the sustainable development of my country's chrysanthemum production, this standard is specially formulated. 1 GB
Auricularia auricula strains
GB 19169-2003
This standard specifies the terms and definitions, quality requirements, test methods, inspection rules and labeling, marking, packaging, transportation, etc. of Auricularia auricula strains.
This standard applies to the production, circulation and use of Auricularia auricula strains. 2 Normative references
The clauses in the following documents become clauses of this standard through reference in this standard. For all referenced documents with dates, all subsequent revisions (excluding the contents of the contribution) or static versions shall apply to this standard. However, the parties to the agreement based on this standard shall consider whether the latest version of this document shall apply. For all referenced documents without dates, the later versions shall apply to this standard. GB/T151 Packaging and transportation mark (GB/T1912000, eq[S0780:1997) B/49, 28 Hygiene microbiological inspection and immunization method, culture medium and test (G3/T127261991 Edible fungi technology
CB 19172-2UH3 Pleurotus ostreatus
NY/T5282002 Edible fungi production technology specification 3 Terms and definitions
The following terms and definitions apply to this standard: 3.1
Stock culture
The pure culture of seedlings and their subcultures obtained by various methods with strong seedlings and their subcultures. Glass test tubes are used as culture vessels and units. Also called primary culture, test bone culture. [NY/T E28-2002, definition X 3,3]
Precullarespaw
A pure culture of mycelium obtained by transplanting and expanding the mother strain. Usually in a glass bottle or plastic plate or 18cm28cm plastic container:
[NY/T528-2002.Definition 3.4]
Precullarespaw
A pure culture of mycelium obtained by transplanting and expanding the mother strain. Usually in a glass bottle or plastic plate or 18cm28cm plastic container:
[NY/T528-2002.Definition 3.4]
Precullarespaw
A pure culture of mycelium obtained by transplanting and expanding the mother strain. In a glass bottle or plastic plate as a container: The precullarespaw can only be used for cultivation, and can be expanded again.
[NY/T 528-2003.Definition 3. ]
Cash
antagonism
and there are different genetic bases between the colonies to produce cattle no growth zone or form different forms of linear edge phenomenon. [(13172-2003, definition 3.
angular mutation sectarr
some body parts of the four viruses or must cause fat some loss, slow growth, case mycelium surface characteristics of the phenomenon of angular volume: -
GH19*69-20D3
[GR19172CC3, definition 3.
high temperature inhibition can high lempcralured linc edible species in the production process of high temperature adverse effects, the growth of the country appear fluorescence, dark or mycelium become weak phenomenon,: GB191727r03. Standard × 3.6
ear science (primordium)
promerdiwm
black ear not standard small research teeth dry culture medium I but the performance of the British color want to monitor the transparent quality description 3. Day
biological efficacy car biulugiel rffieincy number history culture medium and cultured fruiting body or silk weight ratio (H/13728-199*. Standard 2.1.
motility charucters af varlety
food month south of the state ... 1.2 The requirements of the material should meet the requirements of Table 1. Table 1 The material should be of good quality and clean, and should be able to absorb air and filter bacteria. The total temperature should be within 100℃ and the length of the handle should be (s-5)mox(3-.5)m. The material should be of good quality and clean, and should be suitable for people with low income and poor health. Long, special. A, especially the corner, the hundred edges are neat, the big mixed Rui two drops are not two shrinkages, there are bacteria in the end, the color package is complete, the small koji has unique disappearance to the end: no acid, yes, higher heterotrophic microbiology should meet the requirements of the platform,
world species microbiology requirements
rain effect shape car
Xiang is not gas, the product is cut and improved, there is a transfer test to take off the main rate K speed: stop PDA culture (see the record A), in the theft (26K: 2T: the next model silk 13 large--15 large full 4. 1.2 GH19169—2003 4.1.5 Seeds supplied by seed suppliers must undergo seedling tests to prove that the agronomic traits of the seeds are qualified before they can be used for expansion or publication: 4.2. Original seeds 4.2.1 Container specifications shall comply with 47.1.2 of NY/T528, 22. Heat setting Sensory requirements shall comply with the original seed sensory requirements in Table 3 or be plastic free. |tt||The size of each bottle bag and the distance between the doors (the size of each original seed) and the water content of the seedlings should be within the limit of the seedling growth rate, and the seedling growth rate should be within the limit of the seedling growth rate. The seedling growth rate should be within the limit of the seedling growth rate, and the seedling growth rate should be within the limit of the seedling growth rate. uuj tm
package x15m
long teeth production
white to beige fish, sheep, grow inside the edge of the continuous neat and even culture medium. When the species sits closely, there is no shrinkage, sweat, but there is a little net to brown water taste
allowed for early chemical quality, this color two ear shape fruit has not opened by the species unique to the elimination of the bundle, no danger, real, intention and other abnormal blowing 4.2.4 bacteria or cattle this nature: on the appropriate culture medium, under the appropriate ancestor 86: ± 2:), 40) days 45 days long condition zone. 4.3 dependent species
4.3.1 container specifications should be in accordance with NY/T528-2392 + 4.7.1.3 provisions. 4.3.2 separation requirements should be in accordance with the provisions of Table 4. Sensory requirements for cultivation
Base or reactive
Culture medium surface distance from the bottle mouth
Receive quantity (number of species per cabinet)
Features of the south
Total body of culture medium
Required by the industry
Previous knowledge of the family
1 primordium)
Strong, no dandruff, no cracks
Clean, tight and tight, full of airtightness and bacteria requirements 50m-5mm
Bottle (grid) 50 bottles (bag)
Length Micro-car device
Guang Bao Xia Bei Huang Zao Xia Xiao Mao Qi Mao Jian bacteria shepherd's purse edge appearance common Qi Wu Fei hand change year piece brand species special clinical stupid (whole) some or slightly dry plan may have quantity light tube standard capital ball
May have a few species color not black North Shenzhen stock pressure ear has and this on South science has some Ting Xiang Gui, show fall off, suffer from over and other special 3
GB19169—2003
4.3.3 Microbiological indicators should conform to the research including Table 2, 4.3.4 Bacterial plastic growth rate change: on suitable culture medium, under suitable resistance (2612 it), generally 35 big-40 big growth disease wear device. 5 Sampling
5.1 The sampling of the quality inspection department is accurate and representative. 5.2 Each sample is divided into batches according to variety, road conditions, planting time, original variety, and cultivated variety according to the source of the variety, production method and technology time: the samples to be inspected are randomly picked according to the batch.
5.3 The sampling quantity of mother strains, original strains and cultivated strains is about 10% and 5% of the strain plate, respectively. The sampling quantity is less than 10 (bottles, packaging): more than 10 (bottles>, two-level sampling can be carried out. 6 Test method 6.1 Physiological inspection According to the items in Table 5, the inspection method in Table 5 is used for the inspection items. The quantity of strains raised in the field and the main strains are then tested by biological inspection. These methods are based on the naked eye observation. |Inner eye theory
Inner layer
Inner eye trace
Being species
Check and rectify - can be
Mother species, original species
On the culture medium, the surface of the risk
The plate of good meat
The body of the song
Egg breeding and sex equipment
Divided into words will
Miscellaneous south south fall
Sex in the entity removal》
Product prevention phenomenon and corner fee
Check and run method
Inner sleep rules, to blood
Sexual intercourse production record
Inner armor you
Inner limit viewing meal
Inner eye approval
Inner limit double inspection
Inner limit inspection must be safe|| tt||5 Rescue Bank Notes
Meat Observation
Meat and Species
Observe the water-sealed films of the cultured materials with an optical microscope with a magnification of not less than 1×0 for each item in Table 2. Each sample should have less than 50 fields of view.
6.2.14-Bacterial Examination
Take a small amount of cultured materials suspected of being contaminated with recommended bacteria and inoculate them into PDA medium according to the method of non-causing detection! See Section A.1> on the oblique or flat plate, 21C. 10 2 female cultured materials are large-? Large, with the appearance of variegated colors other than white, or with abnormal balls, each chrysanthemum contaminated material, if necessary, conduct water-sealed film microscopy,
6.2.2 Surface Examination
Link a small amount of cultured materials suspected of being contaminated with recommended bacteria. According to the provisions of 4.8 in 423, the tablets are cultured in the culture liquid for 25-28 days. Observe whether the culture liquid is completely filled with mud and sand. The liquid is mixed accurately, and the fine south is dyed: the culture is reduced and the tide is transported. 6.3 Mycelial growth rate 6.3.1 Mother species PD4 plug base (now A, 1 amount), 2F: soil 2T culture, record the number of days to grow full. B19169—2003 6.3.2 Original species and cultivated species are used according to the formula of B.2, B.3, and cultured in 26: soil 2Y: culture, and record the number of days required for full growth, 6. 4 Agronomic characteristics of mother varieties
The seeds will be made into stock during the process. Use one of the matching pieces specified in Chapter K1, Section 13.2, and Chapter B.3, raise the water content to 62%12%, make 15 pieces. After inoculation, carry out routine management in three days, keep records of cultivation, and statistically test the results. At the same time, the seedlings of the mother variety are used as controls and the same treatment is performed. Compare the test results of the two, and use the test items calculated by the time. If any one of the items of the mother variety tested is delayed for more than five days (including five days) during the time calibration of the control, it is unqualified. If the yield is significantly lower than that of the control, it is unqualified. If the surface morphology of the pieces is significantly different from that of the control or the shape is different, it is unqualified. Table 6 Agronomic trait test records in mother seed cultivation
Natural enemies required for 5 kinds of long-selling glycosides
Days required for the original K-selling bottle
Days required for the level of the already and not produced
Days required for the original and strong varieties not produced
Rescue and cultivation department refers to the species of empty fixed embryos and requires large grains
Fearful species of technology species to produce local ear burial device dog society species to harvest department·ear required large society
Trial Ya knot buy
Yield/g||tt| |Single ear quality blue/
Biological efficiency/(%)
Ear shape, color drop
Ear diameter u
Ear tablet rain layer
Test results
6.5 Sampling
All levels of sample collection should be sampled, and the number of samples left should be 3 (bottles, bags)~5 (bottles, bags) for each batch of strains, 10~70% of the mother strain and source strain 6 pieces, and the strains should be cultivated for 4 months. ? Inspection rules
Inspect according to quality requirements. According to the inspection items, all the strains that meet the quality requirements are qualified strains; those that do not meet the requirements in any one of them are unqualified strains.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.1 Phage inspection
Carry out according to the items in Table 5,
Table 5 official inspection methods
Inspection items use
Specifications, no ladder plastic jade
Cultivate and raise
Bacteria strain cattle
Replant main strain
Biological inspection
Some methods
Inner things
Nude observation
Inner eye frequency
Inner eye
Inner layer
Inner eye trace
Being cultured
Mother strain, original strain
Culture medium surface
Plate good meat
The characteristics of the body are as follows:
egg breeding and sex conditions
divided into two parts,
miscellaneous south south falls
sex in the entity removal》
product prediction phenomenon and corner fee
check running method
inside sleeping rules, to blood
sexual intercourse production record
inside armor you
inside limited observation
inside eye approval
inside limited double observation
inside limited current observation must be safe
5 rescue silver reform coupons
meat prosperous observation
meat with the system
table 2 items are observed on the water-sealed slices of the cultured objects with an optical microscope with a magnification of not less than 1×0, and each sample should show less than 50 fields of view.
6.2.14- Bacteria inspection
Take a small amount of suspected bacterial contamination culture and inoculate it into PDA medium according to the procedure described in Section A.1> on the slant or plate. 21C. 10. 2 female culture medium is large. If there are variegated colors other than white or abnormal balls, it is considered as contaminated. If necessary, conduct water-sealed slide microscopy.
6.2.2 Surface inspection
Take a small amount of suspected bacterial contamination culture and inoculate it into PDA medium according to the procedure described in Section 4.8 of Section 423. Cultivate it in water-soluble medium for 25-28 hours and observe whether the culture is clean. Add and mix liquid, for the preservation of fine south dyeing: culture and reduce production, especially tide Bo transport dyeing,
6.3 Mycelial growth rate
6.3.1 Mother species
PD4 blocking base (now A, 1 amount), 2F: soil 2T culture, record the number of days to grow full of the market. B19169—2003
6.3.2 Original species and cultivars
Use the formula of T1, B.2, B.3, and the formula of the single standard, in 26: soil 2Y: culture, record the number of days required for full growth culture,
6. 4 Agronomic characteristics of mother species
The kernel will be made into original species. Use one of the preparations specified in Chapter K1, Chapter 13.2, Chapter B.3, raise the water tray to 62%12%, make, and complete 15. After inoculation, routine management was carried out in three days, and the cultivation records were kept and the test results were statistically analyzed. At the same time, the seedlings of the mother strain were used as the control and the same treatment was performed. The test equipment of the two was compared to calculate the test items. If any one of the test items of the mother strain was delayed for more than five days (including five days), it was unqualified. If the yield was significantly lower than that of the control, it was unqualified. If the appearance and surface morphology of the test piece were significantly different from that of the control, it was unqualified. Table 6 Agronomic trait test records in mother seed cultivation
Natural enemies required for 5 kinds of long-selling glycosides
Days required for the original K-selling bottle
Days required for the level of the already and not produced
Days required for the original and strong varieties not produced
Rescue and cultivation department refers to the species of empty fixed embryos and requires large grains
Fearful species of technology species to produce local ear burial device dog society species to harvest department·ear required large society
Trial Ya knot buy
Yield/g||tt| |Single ear quality blue/
Biological efficiency/(%)
Ear shape, color drop
Ear diameter u
Ear tablet rain layer
Test results
6.5 Sampling
All levels of sample collection should be sampled, and the number of samples left should be 3 (bottles, bags)~5 (bottles, bags) for each batch of strains, 10~70% of the mother strain and source strain 6 pieces, and the strains should be cultivated for 4 months. ? Inspection rules
Inspect according to quality requirements. According to the inspection items, all the strains that meet the quality requirements are qualified strains; those that do not meet the requirements in any one of them are unqualified strains.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.1 Phage inspection
Carry out according to the items in Table 5,
Table 5 official inspection methods
Inspection items use
Specifications, no ladder plastic jade
Cultivate and raise
Bacteria strain cattle
Replant main strain
Biological inspection
Some methods
Inner things
Nude observation
Inner eye frequency
Inner eye
Inner layer
Inner eye trace
Being cultured
Mother strain, original strain
Culture medium surface
Plate good meat
The characteristics of the body are as follows:
egg breeding and sex conditions
divided into two parts,
miscellaneous south south falls
sex in the entity removal》
product prediction phenomenon and corner fee
check running method
inside sleeping rules, to blood
sexual intercourse production record
inside armor you
inside limited observation
inside eye approval
inside limited double observation
inside limited current observation must be safe
5 rescue silver reform coupons
meat prosperous observation
meat with the system
table 2 items are observed on the water-sealed slices of the cultured objects with an optical microscope with a magnification of not less than 1×0, and each sample should show less than 50 fields of view.
6.2.14- Bacteria inspection
Take a small amount of suspected bacterial contamination culture and inoculate it into PDA medium according to the procedure described in Section A.1> on the slant or plate. 21C. 10. 2 female culture medium is large. If there are variegated colors other than white or abnormal balls, it is considered as contaminated. If necessary, conduct water-sealed slide microscopy.
6.2.2 Surface inspection
Take a small amount of suspected bacterial contamination culture and inoculate it into PDA medium according to the procedure described in Section 4.8 of Section 423. Cultivate it in water-soluble medium for 25-28 hours and observe whether the culture is clean. Add and mix liquid, for the preservation of fine south dyeing: culture and reduce production, especially tide Bo transport dyeing,
6.3 Mycelial growth rate
6.3.1 Mother species
PD4 blocking base (now A, 1 amount), 2F: soil 2T culture, record the number of days to grow full of the market. B19169—2003
6.3.2 Original species and cultivars
Use the formula of T1, B.2, B.3, and the formula of the single standard, in 26: soil 2Y: culture, record the number of days required for full growth culture,
6. 4 Agronomic characteristics of mother species
The kernel will be made into original species. Use one of the preparations specified in Chapter K1, Chapter 13.2, Chapter B.3, raise the water tray to 62%12%, make, and complete 15. After inoculation, routine management was carried out in three days, and the cultivation records were kept and the test results were statistically analyzed. At the same time, the seedlings of the mother strain were used as the control and the same treatment was performed. The test equipment of the two was compared to calculate the test items. If any one of the test items of the mother strain was delayed for more than five days (including five days), it was unqualified. If the yield was significantly lower than that of the control, it was unqualified. If the appearance and surface morphology of the test piece were significantly different from that of the control, it was unqualified. Table 6 Agronomic trait test records in mother seed cultivation
Natural enemies required for 5 kinds of long-selling glycosides
Days required for the original K-selling bottle
Days required for the level of the already and not produced
Days required for the original and strong varieties not produced
Rescue and cultivation department refers to the species of empty fixed embryos and requires large grains
Fearful species of technology species to produce local ear burial device dog society species to harvest department·ear required large society
Trial Ya knot buy
Yield/g||tt| |Single ear quality blue/
Biological efficiency/(%)
Ear shape, color drop
Ear diameter u
Ear tablet rain layer
Test results
6.5 Sampling
All levels of sample collection should be sampled, and the number of samples left should be 3 (bottles, bags)~5 (bottles, bags) for each batch of strains, 10~70% of the mother strain and source strain 6 pieces, and the strains should be cultivated for 4 months. ? Inspection rules
Inspect according to quality requirements. According to the inspection items, all the strains that meet the quality requirements are qualified strains; those that do not meet the requirements in any one of them are unqualified strains.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.1> Slant or flat plate, 21C. 10 2 female culture Philippine? Large, with the emergence of white other than the color, or the distribution of strange balls for each chrysanthemum contamination, if necessary, water seal microscopy,
6.2.2 fine surface trace
linked to a small amount of bacterial contamination of the culture medium according to the operation of the /423 4.8 provisions of the tablet culture meat containing culture solution 25--28 days after the waterlogging, observe the growth of the wave is clear of mud and sand. The liquid is mixed, for the preservation of fine south infection: culture and reduce the production of the tide Bo transport infection,
6.3 hyphae growth rate
6.3.1 mother strain
PD4 plug base (now A, 1 amount), 2F: soil 2T culture, record the number of days to grow full of money - surface. B19169—2003
6.3.2 Original seeds and cultivars
Use the formula of Section 1, Section 2, Section 3, and Section 4, and cultivate them in 26: 24: 36: 18: 27: 19: 2 ... Compare the two inspection stations, and use the inspection items calculated by pairs. Any one of the mother varieties tested is more than five days in length. If the delay in the control fungus bed is greater than that of the control, it is unqualified. If the yield is significantly lower than that of the control, it is unqualified. If the surface morphology of the actual year is significantly different from that of the control or follows the shape, it is unqualified. Table 6 Inspection records of agronomic traits in mother varieties
5 kinds of long-smelling glycosides required natural enemies
Original Chong K-smelling bottle required days version
Already and not required days level
Original and strong varieties not required days to break
Rescue cultivation department index species empty index fetus required large grain
Fear name species technology species to produce local ear burial device dog society species to harvest department ear required large society
Trial Ya knot buy
Yield/g||tt| |Single ear quality blue/
Biological efficiency/(%)
Ear shape, color drop
Ear diameter u
Ear tablet rain layer
Test results
6.5 Sampling
All levels of sample collection should be sampled, and the number of samples left should be 3 (bottles, bags)~5 (bottles, bags) for each batch of strains, 10~70% of the mother strain and source strain 6 pieces, and the strains should be cultivated for 4 months. ? Inspection rules
Inspect according to quality requirements. According to the inspection items, all the strains that meet the quality requirements are qualified strains; those that do not meet the requirements in any one of them are unqualified strains.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture mediumWww.bzxZ.net
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.1> Slant or flat plate, 21C. 10 2 female culture Philippine? Large, with the emergence of white other than the color, or the distribution of strange balls for each chrysanthemum contamination, if necessary, water seal microscopy,
6.2.2 fine surface trace
linked to a small amount of bacterial contamination of the culture medium according to the operation of the /423 4.8 provisions of the tablet culture meat containing culture solution 25--28 days after the waterlogging, observe the growth of the wave is clear of mud and sand. The liquid is mixed, for the preservation of fine south infection: culture and reduce the production of the tide Bo transport infection,
6.3 hyphae growth rate
6.3.1 mother strain
PD4 plug base (now A, 1 amount), 2F: soil 2T culture, record the number of days to grow full of money - surface. B19169—2003
6.3.2 Original seeds and cultivars
Use the formula of Section 1, Section 2, Section 3, and Section 4, and cultivate them in 26: 24: 36: 18: 27: 19: 2 ... Compare the two inspection stations, and use the inspection items calculated by pairs. Any one of the mother varieties tested is more than five days in length. If the delay in the control fungus bed is greater than that of the control, it is unqualified. If the yield is significantly lower than that of the control, it is unqualified. If the surface morphology of the actual year is significantly different from that of the control or follows the shape, it is unqualified. Table 6 Inspection records of agronomic traits in mother varieties
5 kinds of long-smelling glycosides required natural enemies
Original Chong K-smelling bottle required days version
Already and not required days level
Original and strong varieties not required days to break
Rescue cultivation department index species empty index fetus required large grain
Fear name species technology species to produce local ear burial device dog society species to harvest department ear required large society
Trial Ya knot buy
Yield/g||tt| |Single ear quality blue/
Biological efficiency/(%)
Ear shape, color drop
Ear diameter u
Ear tablet rain layer
Test results
6.5 Sampling
All levels of sample collection should be sampled, and the number of samples left should be 3 (bottles, bags)~5 (bottles, bags) for each batch of strains, 10~70% of the mother strain and source strain 6 pieces, and the strains should be cultivated for 4 months. ? Inspection rules
Inspect according to quality requirements. According to the inspection items, all the strains that meet the quality requirements are qualified strains; those that do not meet the requirements in any one of them are unqualified strains.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.5. Samples
The sample collection departments at all levels shall collect samples from each batch of bacteria, and the number of samples to be kept shall be 3 pieces (bottles, bags) to 5 pieces (bottles, bags), and the mother strain and source strain shall be 6 pieces, and the strain shall be cultivated for 4 months. ? Inspection rules
Inspect according to the quality requirements. The strains that meet all the quality requirements on the inspection date shall be qualified; the strains that do not meet any of the requirements shall be unqualified.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.5. Samples
The sample collection departments at all levels shall collect samples from each batch of bacteria, and the number of samples to be kept shall be 3 pieces (bottles, bags) to 5 pieces (bottles, bags), and the mother strain and source strain shall be 6 pieces, and the strain shall be cultivated for 4 months. ? Inspection rules
Inspect according to the quality requirements. The strains that meet all the quality requirements on the inspection date shall be qualified; the strains that do not meet any of the requirements shall be unqualified.
Date label, mark, package. Transportation, purchase date, 1 label mark
White, 1.1 product label
Each piece of collection (replacement) must be affixed with a label clearly indicating the following: a: Product name (: Black Fungus Mother Plant)
b Product name (such as 1):
Product name (: × Yi fungus plant): el
2) Inoculation period (: 2001.)
! Execute the label.
8.1.2 Packing label
The packaging label must clearly indicate the following: a) producer name, variety name
b) factory name, address, contact number:
Guarantee period card conditions;
GB19169—2003
number or;
implementation mark,
8.1.3 Packing transportation mark
According to GB/T1U1, there are the following graphic marks: Handle with care mark:
Prevent from sunlight, moisture and freezing mark!
Ensure the packaging, high-yield mark;
Ensure punctuation
Ensure key mark,
8.2 Packaging
8.2.1 The outer packaging of the mother seed is a box or a carton with sufficient pressure. The inside is filled with light materials with general impact resistance such as cotton and paper.
White. 2.2 The original seed and the seed agent are packed in a carton with sufficient pressure. The inside of each carton is filled with light materials with general impact resistance such as milk powder and newspaper. The top and bottom of the carton are sealed with 8cm wide tape, and the same packing tape is used. The box is enclosed with a production certificate and a description of the product (including the formula of the product and the applicable application). 8.3 Transportation
3.1 It shall not be mixed with other items.
日.3.2 When the air temperature reaches above 3℃, use 0℃20℃ refrigerated trucks and ships for transportation: 8.3.3 During transportation, measures must be taken to prevent cysts, fungi, and contamination. 8.4 Purchase and storage
日.4.1 Generally, the mother plant should be stored for one year ~ the first year, and the storage period should not exceed three months. 8.4.2 The original god should be stored in a cold storage above 10℃. The storage period for small plants is more than 40 days: 8.4.3 The seeds should be used as soon as possible: Within 14 days, they can be stored in a loose room with no ventilation, dry light (relative humidity must be ~50%) and no light.
Appendix A
(Normative Appendix)
Cultivar medium and its formula
A,1PDA medium (sodium dextrose agar medium) 200g potato starch (old), 20g glucose, 20g agar, 1000ml water. pH value natural. A,2CFDA medium (compounded yellow potato agar medium) GH19169-2003
200g potato starch (old), 2Ug dihydrogen dextrose, 0.1g magnesium sulfate, 1000ml water. pH value natural A.3 Potassium sucrose medium 200g potato starch (old), 50g sucrose (old), 20g sugar, 1000ml water. pH value natural. Record
(Normative Appendix 1
Culture medium and its formula for original species and cultivated species B.1 Cotton seed culture medium
Tree bark 78%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.2 Cotton seed culture medium
Tree bark 63%, cotton 15%, 20%, 1%, gypsum 1%, moisture content 55%:2%, B.3 Cotton seed culture medium
Cotton husk 79%, 20%, 1%, moisture content 8±2%.
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