Some standard content:
National Standard of the People's Republic of China
Food additive
Isomerized lactose liquid
Food additive
Lactulose liquid
UDC647.918:664
GB 8816—88
This standard applies to isomerized lactose liquid made from lactose as raw material and sodium hydroxide as isomerizing agent. This product is a proliferation factor of bifidus, mainly used in fresh milk, milk powder, biscuits and other foods, and has the functions of helping digestion and absorption of protein and lactose, and producing vitamin B group.
Isomerized lactose contains four kinds of sugars, namely: lactulose, lactose, galactose, and fructose. Among them, lactulose, also known as lactulose, lactose, and galactosyl fructose, plays a role in the proliferation of bifidus. The structural formula of lactulose:
Molecular formula C, H2O
Molecular weight 342.30 (according to the 1983 International Atomic Weight Table) 1 Technical requirements
1.1 Appearance: The first-grade product is a yellow transparent liquid. The second-grade product is a brown transparent liquid. 1.2 Isomerized lactose liquid shall meet the following requirements: Batch name
1. Specific gravity v8
2. Refractive index 20, C
3. Lactulose content, %
4. Lactose content, %
5. Galactose content, %
6. Fructose content, %
Approved by the Ministry of Commerce of the People's Republic of China on February 12, 19882
First-class product index
Second-class product index
Implemented on July 1, 1988
Batch name
9. Total bacteria count, pieces/mL
10. Coliform bacteria, pieces/100mL
11. Pathogenic bacteria (referring to intestinal pathogenic bacteria and pathogenic cocci) 2 Test method
The water used in this test is distilled water.
2.1 Determination of specific gravity
GB 8816--88
Determine by the specific gravity bottle method in GB5009.2-85 "Determination of specific gravity of food". 2.2 Determination of refractive index
According to QB79881 "Determination of refractive index". First-class product indicators
2.3 Determination of lactulose, lactose, galactose and fructose content in isomerized lactose solution (high performance liquid chromatography) Reagents and solutions
2. 3. 1. 1
2. 3. 1. 2
Standard lactulose USP.
Standard lactose (HG 3--1 000-76).
Standard galactose (HG3--1093-77). Standard fructose.
Acetonitrile (HG B 3329-60).
High performance liquid chromatograph.
Chromatographic separation column: Carbohydrate analysis column (3.9×300mm). Detector: Differential refractive index detector.
Recorder: 730.
Micro syringe: 100 μL.
Chromatographic analysis conditions
Analysis operating temperature is 22℃±2.
2. 3. 3. 1
Mobile phase is: acetonitrile: water = 77:23 (V/V). 2.3.3.3 The flow rate of mobile phase is 1.0mL/min. 2.3.4 Chromatographic analysis procedure
2.3.4.1 Preparation of standard solution
Secondary product index
0. 000 05
Not to be detected
Not to be detected
Accurately weigh 2 g of lactulose, 2 g of lactose, 1 g of galactose, and 1 g of fructose in 2.3.1 (accurate to one ten-thousandth of a gram). Dissolve the four sugars in a 100 mL volumetric flask, add water to the mark as a stock solution, filter with a 0.2 μm ultrafiltration membrane, and store. Prepare a standard mixed solution according to different samples during analysis.
2.3.4.2 Sample preparation
Take three 10mL volumetric flasks, wash them, dry them in a 110℃ oven to constant weight, weigh them (accurate to one ten-thousandth of a gram), add the isomerized lactose solution to the scale, weigh them (accurate to one ten-thousandth of a gram), then wash them into 50mL clean volumetric flasks with distilled water, add water to the scale, and filter them with a 0.2μm ultrafiltration membrane.
2.3.4.3 Preparation of working curve
GB8816--88
Inject the standard mixture of four sugars with a 10μL syringe and analyze: draw a working curve with the area of the component peak versus the corresponding concentration (figure omitted). The experiment shows that when the monosaccharide is lower than the following amount, that is, fructose 800μg, galactose 1200, lactulose 1000μg, lactose 1000μg, the detector response (expressed as peak area) has a good linear relationship with the component content. 2.3.4.4 Qualitative and quantitative methods
The injection volume of standard sugar and sample is 10uL. The determination of sample needs to be cross-conducted with the standard sugar mixture, and the retention time of the component peaks in the standard sample and sample is compared for qualitative determination. By calculating the peak area of each component in the standard sugar mixture, a working curve is drawn for the corresponding concentration to find out the concentration of the measured component.
2.4 Determination of arsenic
Take 10g of sample, digest it according to the food digestion method with high sugar content in the nitric acid-perfluoric acid sulfuric acid method, and then determine it according to the arsenic spot method specified in the hygienic physical and chemical inspection method of GB5009.11-85 "Determination of total lead in food". 2.5 Determination of lead
Take the digested solution in the arsenic determination item and determine it according to the dithiothreitol method specified in the hygienic physical and chemical inspection method of GB5009.12-85 "Determination of lead in food".
2.6 Bacterial index inspection shall be carried out in accordance with the determination of total bacteria in Chapter 1, Section 1, the inspection of Escherichia coli in Section 2, the inspection of enteric pathogenic bacteria in Chapter 2, Section 1, and the inspection of Staphylococcus and Streptococcus in Section 2 of the Microbiology Section of the "Food Hygiene Inspection Methods" of the People's Republic of China. 3 Acceptance rules
3.1 Isomerized lactose liquid shall be inspected by the quality inspection department of the manufacturer. The manufacturer shall ensure that all isomerized lactose liquid shipped out of the factory conforms to this standard. Each batch of products shipped out of the factory shall be accompanied by a certificate of conformity in a certain format. 3.2 The user unit has the right to inspect whether the received isomerized lactose liquid conforms to this standard in accordance with the acceptance rules and test methods specified in this standard.
3.3 Use a clean and dry glass tube to take samples from 10% of the packaging materials in each batch. When sampling, insert the glass tube vertically into the container, stir evenly, and then take samples. The total amount of each batch of samples shall not be less than 200mL. Put the samples into two clean ground-mouth stopper glass bottles, mix evenly, and stick labels. Indicate the manufacturer's name, batch number, sampling date, one bottle is sent to the laboratory for analysis, and one bottle is sealed and stored in a dark place for 2 months for future reference.
3.4 If one of the indicators in the test results does not meet the standard, the isomerized lactose liquid should be taken out from twice the number of packages for re-testing. In the results of the re-test, even if only one indicator does not meet the national standard, this batch of isomerized lactose liquid should be deemed unacceptable. 3.5 If the supply and demand parties have objections to the quality of the isomerized lactose liquid and arbitration is required, the arbitration institution can be selected by negotiation between the two parties, and the arbitration should be conducted in accordance with the acceptance rules and inspection methods stipulated in this standard. 4 Packaging, marking, storage, and transportation
4.1 The food additive isomerized lactose liquid should be packed in non-toxic polyethylene plastic barrels or glass bottles for food, and packaged in cartons on the outside, with a net weight of 5kg per barrel and 20kg per box.
4.2 The outer packaging should be marked with the following: manufacturer name, product name, production date, quality standard, net weight, batch number, standard number and "food additive" sample. bzxZ.net
4.3 Isomerized lactose liquid is a food additive. It is strictly forbidden to be placed together with acids, alkalis, toxic substances and other easily corrosive substances. During storage and transportation, it should be avoided from being contaminated by toxic substances. It should be stored in a cool and dark place and not be placed upside down. 4.4 Shelf life of secondary products: not more than 4 months. Additional instructions:
This standard was proposed and coordinated by the Science and Technology Department of the Ministry of Commerce and the Food Sanitation Supervision and Inspection Institute of the Ministry of Health. This standard was drafted by the Liaoning Provincial Commercial Science and Technology Research Institute and the Liaoning Provincial Food Sanitation Supervision and Inspection Institute. The main drafters of this standard are He Fuxiang, Liu Jizhu and Zhang Lixu. 526
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