This standard specifies the detection method and reagents for mouse adenovirus (MAd). This standard is applicable to the detection of mouse MAd. GB/T 14926.27-2001 Detection method for mouse adenovirus in laboratory animals GB/T14926.27-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of Chinabzxz.net
GB/T 14926.27-2001
Laboratory Animals
Microbiological Examination Methods (4)
Laboratory AnimalsMicrobiological Examination Methods2001-08-29 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926-27-2001
This standard is a revision of GB/T14926.27—1994 Laboratory Animals Adenovirus Examination Method. The examination method has not been changed, and only some parts of the original standard have been modified. This standard was proposed and approved by the Ministry of Science and Technology of the People's Republic of China. The drafting unit of this standard is the Chinese Society of Laboratory Animals. The main drafter of this standard is the author of the drafting of this standard.
This standard was first issued in January 1994.
National Standard of the People's Republic of China
Laboratory animalMethod for examination ofmouse adenovirus (MAd)
This standard specifies the detection method and reagents of mouse adenovirus (MAd). This standard is applicable to the detection of mouse MAd.
Cited Standards
GB/T14926-272001
Recommended GB/T14926-27-1994
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is published, the versions shown are valid, all standards will be revised, and the parties using this standard should explore the possibility of using the latest versions of the following standards. GB/T14926.50- 2001
GB/T14926.512001
GB/T14926.522001
Laboratory animals
Laboratory animals
Laboratory animals
Enzyme-linked immunosorbent assay
Immunoalcohol test
Immunomicron test
According to the principle of immunology, MAd antigen is used to detect MAd antibodies in mouse serum: 4 Main reagents and equipment
4-1 Test
4-1.1 ELISA antigen
4-1-1-1 Specific antigen
MAd Inoculate mouse embryonic (ME) or mouse bone (MK) or 3T3 cells, add vitrectomy and culture for 4-5 days, and harvest when the cell lesions reach 10+~10+. After freezing and thawing three times or ultrasonic treatment, remove the cell fragments by low-speed centrifugation, and concentrate the supernatant by ultracentrifugation to prepare or ELISA antigen
4.1.1.2 Normal antigen
ME or MK or 3T3 cells are frozen and thawed, and the supernatant obtained by low-speed centrifugation to remove the cell fragments is stored, 4-1-2 Antigen
MAd infected cells, culture for 3-5 days, when the lesions reach 10+~+++, use brain enzyme to digest and disperse. Wash with PBS and smear. After drying at room temperature, fix it for 10 minutes and store it at -20℃. 4.1.3 Positive serum
Sergeous serum obtained by immunizing SPF mice with MAd antigen. 4-1-4 Negative serum
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 2001 and implemented on May 1, 2002
SPF mouse serum,
4.1.5 Enzyme-bound
GB/T14926-27—2001
Spicy peroxide-labeled sheep or rabbit anti-mouse IgG antibody: or thin root peroxide-labeled staphylococcus A
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