This part specifies the methods and requirements for field efficacy tests of fungicides against tobacco brown spot pathogen (Alternaria alternata). This part applies to the field efficacy plot test and evaluation of fungicides for registration against tobacco brown spot pathogen. Other field efficacy tests shall refer to this part. GB/T 17980.91-2004 Guidelines for field efficacy tests of pesticides (II) Part 91: Efficacy tests of fungicides against tobacco brown spot pathogen GB/T17980.91-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and requirements for field efficacy tests of fungicides against tobacco brown spot pathogen (Alternaria alternata). This part applies to the field efficacy plot test and evaluation of fungicides for registration against tobacco brown spot pathogen. Other field efficacy tests shall refer to this part.

GB/T'17980.91—2004www.bzxz.net
The efficacy test is one of the important contents of pesticide registration management and is the important technical basis for the preparation of product standards. The standard is the only guide for the safe and reasonable use of pesticides. In order to standardize the methods and contents of daytime pesticide tests, make the tests more scientific and unified according to international standards, and make our country's efficacy test products have internationally recognized conditions, we have specially formulated national standards for test standards. This series of standards refers to the European and American Plant Protection Organization (EOPW) field efficacy test standards and the United Nations Agriculture and Rural Affairs Organization (FA) Asia-Pacific region similar pesticide standards. It is formulated based on my country's actual situation and verified by rat daytime efficacy tests. Tobacco red blight is an important tobacco disease in my country. In production, it is often necessary to use pesticides for prevention and control. In order to determine the use of pesticides for the prevention and control of red blight, the test drugs are specially designed to identify crops and non-target organisms with the impact of antimicrobial agents. This part is specially formulated to provide a basis for efficacy evaluation and safety, and to provide a basis for the use of technology. This part is one of the second series of standards for pesticide field efficacy test criteria. This part is an independent part and was proposed by the Ministry of Agriculture of the People's Republic of China. Drafting unit of this part: Pesticide Regulation Institute of the Ministry of Agriculture. The main contributors to this part are: Liu Baoan, Wu Xinping, Gu Baoshuai, Kong Fan, Wu Xiaobo, and Sun Huatian. This part is under the responsibility of the Pesticide Regulation Institute of the Ministry of Agriculture. 1 Standard for Field Efficacy Tests (II) Part 1: Fungicides for Control of Tobacco Red Spot Disease CB/T17980.91—2004 Part 2 defines the fungicides required for the prevention of tobacco red spot disease ( Aiternariaaite/7ztt) The method and requirements of the efficacy test of the fourth section are applicable to the field efficacy trials and evaluation of ten strains of bacteria for the prevention and control of red rot in the state. Other field efficacy trials shall be carried out in accordance with this section.
2 Test conditions
2.1 Selection of test objects, crops and varieties. The test objects are not susceptible to early disease.
The test crops are tobacco. Select the susceptible varieties NC89 and NC2. Record the variety name. 2.2 Environmental conditions
The field trials should be carried out in areas where the disease has occurred. The cultivation conditions of all test plots (such as soil type, crop variety, seed, transplanting period, plant row, etc.) should be consistent. 3.1.1.1. The test agent shall indicate the trade name and product name of the test agent, including the Chinese name, dosage form and manufacturer. The test agent shall be used in no less than three doses in the test agreement (the test agent dosage specified in the test agreement signed by the test committee and the test undertaker). 3.1.2. The control agent shall be a registered product that has been proven to be effective in practice. The type and form of the agent shall be consistent with the test agreement and traceable to the commonly used methods. The specific conditions may depend on the test date. 3.2.2. 1 Plot arrangement
The plots for the experimental drug, control drug and blank control are randomly arranged. Special circumstances should be explained. 3.2.2 Plot area and annual sales
Plot area: 15m~50m.
Number of spraying: at least 4 times.
3.3 Application method
3. 3, 1 Instructions for use
Follow the requirements and label instructions: Application should be consistent with local scientific agricultural practices. 3.3.2 Type of equipment used
Select the equipment commonly used in production, record the type of equipment used and the complete information of the operating parameters (pressure, spray diameter, etc.), and ensure that the amount of medicine is evenly distributed. The requirements for the weight of the medicine should be kept in mind. 3.3.3 The time and frequency of application of pesticides shall be carried out in accordance with the requirements of the agreement and the newly signed instructions. Usually, the first application of pesticides shall be carried out when the disease first occurs, and further determined according to the development of the disease and the duration of the pesticide. Record the date of application and the test period. 3.3.4 Dosage and volume used shall be used in accordance with the requirements of the agreement and the dosage indicated in the standard. The effective dosage of the pesticide is usually expressed as g/h:n. The monthly multiple and the required dosage (L/) shall be recorded.
3.3.5 Data requirements for other pesticides for controlling diseases and insect pests If other pesticides are to be used, they should be selected to have no adverse effects on the test pesticides and the test objects, and all small winds should be kept separate from the test pesticides and the pesticides to minimize the interference of these pesticides. Accurate data on the application of these pesticides should be recorded.
4 Investigation, recording and measurement methods
4.1 Weather and soil materials
4.1. 1 Meteorological data
Data on rainfall patterns (rainfall patterns and rainfall base, expressed in m) and daily average temperatures (expressed in h) should be obtained from the nearest meteorological station at the test site during the test period. The weather patterns that affect the test fruits during the entire test period, such as dry spells, storm surges, and ice balance during the growing season, should all be recorded. 4.1.2 Soil filling materials
Record soil type, soil fertility, moisture content (as follows, humidity as follows) and soil moisture content (as follows, humidity as follows). 4.2 Survey methods, time and frequency 4.2.1 Survey methods Five sampling points in the plot every year, random survey at 2 points every year, record the total number of leaves and the number of diseased leaves at each point, and grade the method: Grade 1, including diseased plants; Grade 2, the area of ​​diseased plants accounts for more than 1% of the total area. The following 3 levels: the area of ​​lesions accounts for 2~3% of the leaf area; Level 2: the area of ​​lesions accounts for 5%-10% of the leaf area; Level D: the area of ​​lesions accounts for 11~20% of the leaf area; Level D: the area of ​​lesions on the leaf surface is more than 21 points; 4.2.2 Adjust the time and frequency of the change according to the technical agreement. The number of disease symptoms of the fungus used for the bundle application shall be shown before the next application and after the next application. 4.2. 3 Calculation method of drug efficacy
Disease index 2 Relative value of each level of leaf formation 11. Total adjustment × 9
Blank control area disease index before drug × treatment area disease index after drug × 13 (2) Control efficacy (%) - [
Blank control area disease index after drug × treatment area disease index before drug or control effect (no secretion number before application "%) - blank, self-transmitted disease index × 100 (3) White control area disease index
4.3 Direct impact on crops
Observe whether the drug has any effect on crops. If there is any damage, record the type and degree of damage. In addition, the beneficial effects on crops (such as stimulating growth, promoting maturity, etc.) should also be recorded. Use the following method to record pesticide damage:
Effect of the fruit is measured or calculated, and it should be expressed in terms of tensile values, such as plant height s
In other cases, the following two methods can be used to estimate the frequency of pesticide damage: b
GB/T 17980,91-2004
1) According to the pesticide classification method, record the severity of pesticide damage in each plot. Use one, ten, ten, and ten: one ten ten ten ten. Pesticide damage classification method:
-, one, ten, ten:
Ten: Mild pesticide damage: Does not affect the normal growth of crops! 10: Obvious pesticide damage. Can be recovered, will not cause crop yield reduction. 11: Severe pesticide damage, affecting crop growth, causing a certain degree of loss to crop yield and quality: generally require compensation for part of the economic loss. 12) Severe pesticide damage, crop growth is affected, production is heavy and pressure is severe, must be compensated for losses, 1) Compare the pesticide treatment area with the control area, evaluate the percentage of pesticide abandonment, accurately describe the symptoms of the crop (chemical, green, shape), and provide physical photos, videos, etc. 4.4 Effects on other organisms
4.4.1 Effects on other diseases and pests
Any effects on other pests and diseases should be recorded. 4,4.2 Effects on other non-target organisms
Record the effects of the pesticide on wild animals and beneficial insects in the test area. 4.5 Product performance and quality
Record product quality.
5 Results
The test results should be analyzed by biostatistical method (DMRT method should be adopted). The test results should be analyzed in a formal report format. The original materials should be kept for inspection and verification.
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