This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of pike. This standard is applicable to the detection and identification of pike germplasm. GB 19162-2003 Pike GB19162-2003 standard download decompression password: www.bzxz.net

ICS 65.150
National Standard of the People's Republic of China
GB19162—2003
Mullet
2003-06-04 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
2003-12-01 Implementation
Chapter 4 and Chapter 6 of this standard are mandatory, and the rest are recommended. Appendix A of this standard is an informative appendix.
The technical standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the Marine Aquaculture Sub-Technical Committee of the National Technical Committee for Aquatic Products Standardization. The drafting unit of this standard: Tianjin Fisheries Research Institute. GB19162—2003
The main drafters of this standard: Li Xiangpu, Ma Weilin, Liu Maoli, Geng Xuyun, Fang Enjun, Wang Zhiqing, Xing Lijian, Sun Jinsheng. 1
1 Scope
GB19162—2003
This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics and detection methods of pike (Murilso-iuy Basilewsky).
This standard applies to the detection and identification of pike germplasm. 2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. For any dated referenced document, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, the parties to the agreement or agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For any undated referenced document, the latest version shall apply to this standard. GB17716 Qing
: 3 Name and classification
3.1 Scientific name
Sakura-horned Mugil sa-iuy Basilewsky3. 2 Classification position
Generic order (Mugiliformes), family (Mugilidac), genus (Mugit). 4 Main morphological and structural characteristics
4.1 External morphological characteristics
4.1.1 Appearance
The body is slender and fusiform, with a slightly flat front end and gradually flattened to the back. The mouth is inferior and in the shape of a "human". There are small teeth on both jaws, which are chalky and velvety. 1 The frontal bone suddenly bends downward behind the corner of the mouth, and the rear end is exposed. The eyes are small orange-red, and the fat eye face is not developed. There are two dorsal fins, separated. There are no axillary scales at the base of the pectoral fins. The body is covered with weak comb scales, the head is round scales, and there is no lateral line. The gills are slender and densely arranged. The tail fin is concave or shallow. The head and dorsal part of the body are hard blue, the sides of the body are light yellow, and the abdomen is white.
The external morphology of the spindle color is shown in Figure 1.
Figure 1 The appearance of the pike
4.1.2 Countable traits
4.1.2.1 Dorsal fin style: D. +T 8,
GB 19162—2003
Pectoral scales style: 1.16~17.
4.1.2.2
4.1.2.3 Pelvic fin style: V. I -5.4.1.2.4 Anal eel style; A, 1-8~9.
4.1.2.5 Number of scales in a row: 36~~44.
4,1.2.6 Number of scales in horizontal rows: 12--14,
Number of gill scales outside the first gill arch: 2G~-60 + 50~～76. 4, 1. 2.7
4.1.3 Measurable traits
The proportion values ​​of measurable traits of the measured individuals with body length 6.0mm~71.0cm and body weight 34g\~5330g are shown in Table 1. Table 1 Ratio of measured measurable traits
Total length/body length" Body length/body height, body length/head length" Head length? Snout length Head length/eye diameter
Head length/distance between eyes
1.186o.0835.031±0.6514.211+0.2454.593=0.4955.707±0.8572.133±0.1994.2 Internal structural characteristics
4. 2. 1 Spiral
Gills are hollow, forked at the front, with angular spines on both sides. 4.2.2 Vertebrae
Total number of vertebrae: 24. The articular process of the second vertebra extends backwards and is spinous in shape. 4.2.3 Peritoneum
Colorful.
4:2.4 Pyloric straight
5~7 (usually 6).
4.2.5 Digestive tract
Esophagus spotted, stomach in "V" shape, kidney wall muscular; intestines long.4, 2. 6 Location of gonads
Back of body, below.
5 Growth and reproduction
5.1 Growth
Length peduncle length/tail peduncle height
Body length/mud habitat lengthbzxz.net
5.13:1+0.403
1, 947+0. 202
The measured values ​​of body length and weight of fish in different age groups are shown in Table 2. The average calculated values ​​of body length and weight of fish in different age groups corresponding to Table 2, growth equation and body length and weight relationship are shown in Appendix A. Table 2 Measured values ​​of body length and weight of fish in different age groups Age/age
Solid length/cm
Measured weight/g
Measured body length/cm
Solid weight/g
17. 3-~35, 2
86~750
23.4-~40.4
101--979
25. 2~-47. 5
250--1 815
29.2~47.6
417--2 075
36. 0---53. 5
510~2665
31, 1~52.1
634 ---2 703
42. 9--66.5
1 205--4 045
44.2~~69. 6
1 440--3 680
Certificate; 1-year-old fish cannot be distinguished by sex - physical length: 9. 5 cm25. 3 rm, measured weight, 26 g~260 g, 5.2 breeding
5.2.1 Sexual maturity age
Female fish 3-4 years old, male fish 2-3 years old.
5.2. 2 Spawning times
The sexually mature individual's gonad matures once a year and spawns once. 2
58. 3--67. 0
2 200 ~4 305
56, 4--64, 5
2 995~4 700
61,0~74, G
3 745~4 590
57, 6~- 71.
2035~5336
5. 2. 3 Egg carrying capacity
The egg carrying capacity of individuals in different age groups is shown in Table 3.
Age/age
Weight/8
Absolute egg carrying capacity/grain
Relative egg carrying capacity/(grain/g)
Genetic characteristics
Cytogenetic characteristics
6.1.1 Chromosome number
1059+i69.6
Table 3 Egg carrying capacity of individuals in different age groups
1 491+695.0
2 596±677.7
CB 19162—2003
2945±73.4
(13.7=3.2)×10(13.0+2.2)×10%(7.51.9)×10(5.5±1.1)×10
(7. 5=1. 6)× 165
The number of chromosomes in the body is 2n=48.
6.1.2 Karyotype
555165. 4
745148.5
411-153.9
260±31.9
13 pairs of centromere chromosomes (m group), 5 pairs of sub-centromere chromosomes (sm group), and 6 pairs of sub-telomeric chromosomes (st group). The number of chromosome arms (NF) is 84 (see Figure 2). Karyotype: 13m+5srn—6st. Ding A
6.2 Biochemical genetic characteristics
Figure 2 Fish chromosome karyotype
6.2.1 Lactate dehydrogenase (LDH) isozymes in muscle 6.2.1.1 Lactate dehydrogenase (LDH) isozyme spectrum in muscle is shown in Figure 3. 6. 2. 1. 2
Lactate dehydrogenase (LDII> isozyme scan in muscle is shown in Figure 4. Figure 3 Electrophoresis spectrum of lactate dehydrogenase (LIH) isozyme in muscle n
Figure 4 Enzyme band scan of lactate dehydrogenase (LDH) isozyme in muscle Figure 6.2.1.3 Lactate dehydrogenase (I.The relative activity intensity of lactate dehydrogenase (LIH) isozyme is shown in Table 4. Table 4 Relative activity intensity of lactate dehydrogenase (LIH) isozyme in pike muscle Alcohol band Relative activity intensity GB 19162-2003 6.2.2 Population genetic variation range The proportion of polymorphic gene loci was 0.50, the average heterozygosity was 0.0727, 7 Inspection methods 7.1 Age identification 7.1.1 Scale selection site: between the lower part of the dorsal fin base and the upper part of the vertical row of scales on the side of the body, 7.1.2 Operation steps; Use a trowel to remove the dorsal scales and store them in the scales. a
Take out the scales stored in the scale bag, place them in culture medium, and clean them. Press the cleaned scales on the fish body and clamp them between two glass slides, attach a marker, and seal the two ends with transparent tape. d) Observe the growth rings of the scales under a microscope or projection to determine the age of the fish. Growth ring characteristics: sparse and dense, cut or form gaps. 7.1.3
7.1.4 Regenerated scales shall not be used for age identification. 7. 2 Determination of fertility
During the breeding season, live dissection of sexually mature broodstock before spawning is performed, and body weight, pure body weight and gonad weight (accurate to 0.01g) are weighed on an electric balance. At the same time, 1.00g of eggs are weighed, and two samples are counted under a dissecting microscope to calculate the average number of eggs per gram. The total number of eggs contained in the nest is the absolute egg-bearing capacity, and the number of eggs contained per unit body weight (g) is the relative egg-bearing capacity. 7.3 Chromosome detection
Perform in accordance with GB17716.
7.4 Biochemical genetic analysis
Perform in accordance with GB17716.
Shanlu A
(Informative Appendix)
The calculated values ​​of body length and weight of fish in different age groups, growth equation, body length and disc relationship A.1 The calculated values ​​of body length and weight
and Table 2 The corresponding average decalculated values ​​of body length and weight of fish in different age groups are shown in Table A.1. Table A.1
Age/(age)
Decalculated body length:/cm
Decalculated weight/name
Decalculated body length/cm
Decalculated weight/g
Growth equation
Male fish:
Female fish:
In the formula:
Average decalculated values ​​of body length and weight of fish in different age groups2
L, -137. 8[1e-3. 087 *t-0.659 61]W, 34 120. 1[1- e-0 87 8(+0. B9 62 L,=121, 2[1e-0. 106 2+,811 )]W, =28 125. 3[1—ec 0 2(+# A > 73L—body length of fish at one age, in centimeters (cm): W, body weight at one age, in grams (g))e (a natural logarithm;
age of fish, in years.
A.3 Body length and surface area relationship
GB 19162—2003
.(A.1)
++..+++++.-+++( A, 4 )
W = 0. 027 09 X I1.3.6Y bh4
In the formula;
W——body weight, in grams (g);
body length, in centimeters (c).
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