NY/T 2569-2014 Guidelines for testing specificity, consistency and stability of new plant varieties Cannabis NY/T2569-2014 |tt||Standard compressed package decompression password: www.bzxz.net

ICS65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T 2569—2014
Guidelines for the conduct of tests for distinctness, uniformity and stability-Hemp
(Cannabis sativa L.)
2014-03-24 Issued
2014-06-01 Implementation
Ministry of Agriculture of the People's Republic of China
Normative citations
Terms and definitions
Symbols:
Requirements for propagation materials
Testing methods
Specificity, consistency, stability, and determination of results 8 Character table
Grouped characters
10 Technical questionnaire
Appendix A (Normative Appendix) Hemp Character Table B (Normative Appendix)
Explanation of Hemp Character Table
Appendix (Normative Appendix) Hemp Technical Questionnaire Format
NY/T 2569—2014
NY/T 2569--2014
This standard is formulated in accordance with the rules given in GJ3/T1.1-2009 and is proposed by the Seed Administration Bureau of the Ministry of Agriculture.
This standard is formulated by the National Technical Committee for Standardization of Testing New Plant Varieties (SAC/T277). The drafting units of this standard are: Economic Crops Research Institute of Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Economic Crops Research Institute of Nanning Academy of Agricultural Sciences, Science and Technology Development Center of the Ministry of Agriculture, The main drafters of this standard are: Xiao Guangwen, Sun Lianfa, Yang Ming, Zhang Liguo, Chen Lijun, Wu Xiexian, Rong You, Tui Yongpian, Fang Yuyan, Zheng Nan, Li Dongmei, Xu Yan, Wu Chaoning, Guo Hongyan, 1 Scope
NY/T2569-2014
Guide for testing specificity, consistency and stability of new plant varieties.
This standard specifies the technical requirements for qualitative testing of specificity, consistency and stability of new varieties and the general principles for judging results. This standard applies to the specificity, consistency and stability test and result judgment of new varieties of hemp (Cazabis safizI.): 2 Normative references
The following documents are indispensable for the application of this document. For all references with dates, only the versions with the dates are applicable to this document. For all references without dates, the latest versions (including all amendments) are applicable to this document. GF/T3543.3 Inspection procedures for crop seeds Purity analysis GB/T3543.4 Inspection procedures for crop seeds Germination test GB/T3543.G Inspection procedures for crop seeds Moisture determination GB/T10557.1 Guidelines for specificity, efficacy and stability testing of new plant varieties General 3 Terms and definitions
The terms and definitions defined in GB/T19557.1 and the following terms and definitions apply to this document. 3. 1
Group measurement single measurement of a group of plants or parts of plunts A group record is obtained by measuring a certain organ or part of a group of plants or plants. 3.2
Individual measurement meusuremenlolanumherofindividualplantsorpartsofplants A group record is obtained by measuring a certain organ or part of a group of plants or plants. 3.3
Visual assessment by a single operator of a group of plants or parts of plants Obtain a group record. 3.4
Visual assessment by observation of individual plants or parts of plants Obtain an individual record. 4 Symbols
The following symbols apply to technical documents:
MG: group measurement.
MS: individual measurement.
VG. Group measurement:
VS: individual visual assessment.
QL: Quality property:
QY: Numerical property,
PQ: Pseudo quality property:
NY/T 2569—2014
(a)-~（d): The annotation content is explained in detail in B.2. (X): The marked content is explained in detail in Table A of B.3. "\S\ is the standard variety used in the southern region, "\ is the standard variety used in the northern region: \SV\ is the standard variety used in both the north and the south.
5 Requirements for propagation materials
5.1 Propagation materials are provided in the form of seeds or young cores. 5.2 The submitted seed quality certificate is at least 0.3g. The submitted seedlings are 0 plants. 5.3 The submitted propagation materials should be healthy in appearance, high in vitality, and free of diseases and insect pests. According to the provisions of G3/T3513.3, (GT/T343.4 and (GB/3513.6, the specific quality requirements of propagation materials are as follows: purity 296.0%. Teething rate 85%, water content 9 %5.4 The propagation materials submitted shall not have been subjected to any treatment that may affect the normal expression of the variety's traits (such as seed coating treatment). If they have been treated, detailed descriptions of the treatment shall be provided.
5.5 The propagation materials submitted shall comply with the relevant provisions of China's plant quarantine. 6 Test method
6.1 Test cycle
The test cycle shall be at least 2 independent growth cycles. 6.2 Test location
The test is usually carried out at one location. If certain traits cannot be fully expressed at this location, they may be observed at other eligible locations.
6.3 Field trial
6.3.1 Experimental design
The variety submitted and similar varieties Near planting, for varieties that have been propagated, the number of plants planted shall not be less than 200, with a spacing of 10 (t120cm and a row spacing of 30ct11~-70cm-repeated twice; for varieties that are propagated asexually, the number shall not be less than 10 plants. 6.3.2 Field management
It can be carried out according to the production management methods used by local people. 6.4 Character observation
6.4.1 Observation period
Character observation should be carried out according to the growth stages listed in Appendix A. The description of the growth stages is shown in Table B. 6.4.2 Observation method
Character observation should be carried out according to the observation methods specified in Appendix A (VG, VS, M, MS). Some character observation methods are shown in I3.2 and 3.|| tt||6.4.3 Number of observations
Unless otherwise specified, the number of plants sampled for individual traits (VS, MS) shall be no less than 10. When observing the organs or parts of the plants, the number of samples taken for each plant shall be 1. For group observations of traits (VG, MG), the entire plot or a mixed sample of a specified size shall be observed. 6.5 Additional tests
If necessary, additional tests may be performed on traits not listed in this document. 7 Determination of specificity, consistency and stability results 7.1 General principles
The determination of specificity, consistency and stability shall be carried out in accordance with the principles determined in (GB/T 3557.1)2
7.2 Determination of specificity
NY/T 2569—2014
The proposed variety should be clearly distinguished from all known varieties. In the test, when the proposed variety has a clear and reproducible difference with similar varieties in at least one trait, it can be determined that the proposed variety has heteromorphism. 7.3 Determination of consistency
The consistency of seed-propagated varieties is determined by comparing with varieties of the same type, that is, the degree of variation of the variety cannot significantly exceed that of varieties of the same type. When evaluating the consistency of asexually propagated varieties, a sample standard of 1 and a probability of acceptance of 95% are used. If a sample consists of 10 plants, a maximum of 2 heteromorphic plants are allowed.
7.4 Determination of stability
If a variety has consistency, it can be considered stable. Generally, stability is not tested. If necessary, the next generation of seeds or the next batch of asexual propagation materials of the variety can be planted. Compared with the previously provided propagation materials, if there is no obvious change in the expression of the trait, the variety is determined to be stable. 8. Trait table
8.1 Overview
Basic traits are the traits that must be used in the total test. The basic traits of cannabis are shown in Appendix A. The trait table lists the trait name, expression type, expression state and corresponding code, standard variety observation period and method, etc. 8.2 Expression type
According to the expression method of traits, traits are divided into three types: quality traits, pseudo-quality traits and quantitative traits. 8.3 Expression status and corresponding codes
8.3.1 Each trait is divided into a series of expression states. In order to define traits and standardize descriptions: Each expression state is assigned a corresponding numerical code. In order to facilitate data recording, processing and the establishment and communication of variety descriptions. 8.3.2 For quality traits and pseudo-quality traits, all expression states should be listed in the test guide; for quantitative traits, in order to shorten the length of the trait table, the expression states of even-numbered codes are not listed, and the expression states of even-numbered codes are described in the form of the previous expression state to the next expression state.
8.4 Standard varieties
The trait table lists standard varieties that can be referenced for the expression states of some traits to help determine the different expression states of related traits and correct the differences caused by environmental factors.
9 Grouped traits
The linear classification of varieties in the combined file is as follows:! Color intensity of the model strain of C. cordifolia (Table A, 1 in 4). h) Plant: ratio of hermaphrodites (Table A.1 neutral trait 13), Plant: ratio of female plants (Table A.1 neutral trait 14) d) Plant: ratio of male and female plants (Table A.1 neutral trait 15) e) Plant: plant height (Table A.1 neutral trait 18) 10 Technical Questionnaire
Applicants should fill in the cannabis technical questionnaire in the format given in Appendix (C), NY/T 2569-2014
A. 1 Basic characteristics of cannabis
See Table A.1
Leaf color
Color intensity
Plant: Heart leaf flower bottom color intensity
Fi. People small
Leaf sodium: Flower back ring color intensity
[ (--)
Flower: Disordered, common, northern, time, hundred
Green degree
Appendix 4
[Normative Appendix]
Table of cannabis properties
Table of basic cannabis properties
Test period
Properties fully described
Slaughter, oval
Medium, obovate
Wide, inverted
No glassy, ​​weak
No or weak
Standard product
Guyuan only, Y
Qiqihar City, SN
Zhang Jing, SN
so1t -1.sv
Yunma No. 4,SN
Yongren,
Shilin 2,SN
Qitian,S
Shilin County-2,5%
Qiqiluoer City,SN
2010-1,Guoyuan County S)
Zhangjiakou.Ni Shilin County Lan,S)
2010 -1,SN
Shilin County-2,SV
.SO31.SN
Zhangjia[1.N (Shilin-2.S)
2019:1.5V
Yanyuan.SV
Serial number (
「Leaf; Center leaflet length
Chuan: Center leaflet width
Petiole: degree
Zhi Le: Ya Xiong same plant ratio||tt| |Plant: Female plant ratio
Plant: Plant ratio
Inflorescence: Flowery back Wulingsiqiang bird
Flower disease: 『HC containing bureau
『First plant: high
Cable: Color
Table A.1 (continued)
Observation period and method
Character description
None or very weak
None to very low
Guard green
NY/T 2569—2014
Standard male species
201C-N(S)
1 Ma No. 1, 1: Youlin County-2, S:
2010-4N Mo H, 3)
Yongren, N Mingqiao Bing·2.S)
USO3I, SN
2010 Yunma
Youlin 2·2.SN
Shenqiha East (SN)
Device,
USOST, SN
, LIS(XI.N(Qilu Luxiang City, S)
201e-1.N(Original. 9)
Lu'an Hanma, N Shilin County-2, S)
2 Lian 4.SY
Lin-2SN
NY/T2569-2014
Base, "Interval length Ling
1 winter: number of bottles
School: number of nodes
, plant: branch length change
Du plant: branch height
Special: branching effect
Base: cross-section of pith thickness
Plant: fall inspection||tt| |1 seed; dry grain
planted: outer seed coat color
Table A.1 (continued)
Viewing time and method
220 yuan
Character selection
None minimum
None or very little
Especially or extremely
Medium gain
Standard variety
Mo N Jiqihar City.S:
Yanyuan.N (Guyuan County.S)
Yuncheng No. 1 N (Bulin County 2,S)| |tt||Qijiha East City.SN
2Assist, NGuoyuan County S)
Shu-2,S
Qijihar CitySN
LusiN Guyuan County S)
In Linshi-2,SV
2010.N (Zhiyuan County, S)
Shitizhizhi2.SV
201-1,NLinshi-2.S)
2C13-2,SN
Linzhizhi2.SN
2010 -: .SN
2011-1. V2 Lin 4, S)
S01.N(H Yuanxian,)
Moyu S
USCS1, SN
Zhangjia 1.sY
Shuirenzhen S
Shexian-2.S
Seeds; Seed coat pattern degree
Table A.1 (continued)
Observation period and method
Character band
None or weak
NY/T2569—2014
Standard product
Zhangliao.sV
USOS2, SN
NY/T2569—2014
B.1 Hemp growth stage
See Table 13. 1:
Appendix B
(Normative Appendix
Explanation of the table of cannabis characteristics
1.1 Basic growth stages of cannabis: The four growth stages in the table represent the entire growth period of the plant. The first digit of the four-digit growth period code represents the basic growth stage of cannabis (Table B.1 Basic growth stages of cannabis
The first digit of the code for the growth period represents the germination period
Nursing period
Flowering and seeding period
Aging period
B.1.2 Secondary growth stage of the plant: The second digit of the four-digit growth period code represents the characteristics of the plant. The third and fourth digits represent the developmental period of the plant (B.2) Table B.2 Secondary growth stage of cannabis plant represents the development period of ten kinds of nutritional growth (Science). Leaf blade refers to the small at least m1362
【1st pair
2nd pair
3 pairs
1st pair of leaves
5 pairs
1st pair of leaves
Flowering and seeding period (color extraction and division)
sexuality through dioecious coal
flower primordium
flower shadow formation period
flower formation period
well flower example lake
"a tender!
3 lazy leaves
5 tender leaves
7 tender +
x2(nth pair)
the main whole leaf sequence changes from pairs to round leaves.cm gender cannot be distinguished
the first closed flower is
the first hybrid male flower blooms
only sexual plants
230 yuan
old period
flowering period
flowering period
flower shadow period
initial flowering
and flowers
the early maturity of Pu Zi
planted maturity period
Seed skin maturity period
Female flower formation period
Female flower early flowering period
General flower flowering milk
Male flower shape
Male flower upper flowering period
Seeds or hot early
Planted in the war maturity period
, planted mature milk
St. sticky
Steamed musk roasted
Explanation involving multiple traits
The last pair of complete plants Fully expanded leaves,
Only observed plants
Table B.2 (continued)
50 Flowers open
95 Males open or poorly
Su Yip Flower
The 7th Styled 5th Flower
30 Photochromic formation
The 9th Seed change
Seed hardening
05 Seed change or scattered
The 10th Styleless Wedding Flower
Stigma initial station
505Female flowers have bracts
The first closed stigma appears
Most male flowers open
The first species hardens
50The first species changes
9The seeds harden or scatter
The sun changes
Leaf holes fall
Wave falls
Observation site is the observation rate above the base of the plant/3.
B.3Explanation of individual characters
Character classification and codes in the figure are shown in Table A.
Character 1: shape, see Figure B:
Nai obovate
About leaf: color, see Figure.?
Character 2wwW.bzxz.Net
Cotyledon: shape
Inverted oval
NY/T 2569—2014
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