Some standard content:
ICS65.120
Agricultural Industry Standard of the People's Republic of China
NY/T722—2003
General standard of feed enzyme preparation2003-12-01Promulgated
Ministry of Agriculture of the People's Republic of China
Implementation on 2004-03-01
This standard was proposed by the Ministry of Agriculture of the People's Republic of China and drafted by the National Technical Committee for Standardization of the Feed Industry of Guangdong Yiduoli Animal Science and Technology Co., Ltd. at its own expense, and the Ministry of Agriculture Feed Quality Supervision and Inspection Center (Guangzhou) participated in the drafting.
The main contributors of this standard are: Chen Shaomei, Luo Daochao, Liu Yali, Biao Jiance and Feng Wenhua. 1 Scope
General rules for enzyme preparations for feed
NY/T722—2003
This standard specifies the technical specifications and definitions, requirements, test methods, test rules and common marking, labeling, packaging and transportation of static preparations for feed. This standard applies to enzyme preparations for feed. 2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. For any referenced document with a date, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, the parties to the latest agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For any referenced document with no date, the latest versions are applicable to this standard. GB3/T-1789.3—1991 Microbiological examination of food - Determination of bacterial flora G3/T6917-198 Determination of particle size of compound feed powder G13/T 643F1386
Determination of moisture in feed
(B10618 Material label
Determination of total arsenic in feed
GB/T 13079 1999
GB/T 13080—1991
GB/T 13082—{991
GI3/T 13091—[99]
Determination of lead in feed
Determination of lead in feed
Testing method for salmonella in feed
Type and method for toxicological evaluation of food safety GB15133,.1199
(G13/1 17280
QH/T 18031993
3 Terms and definitions
Abbreviation Method for determination of aflatoxin B in feed Enzyme-linked immunosorbent assay Test method for enzyme preparations
The following terms and definitions apply to this standard. 3.1
Feed enzyme preparations are produced by enzyme-producing biological fermentation or extraction of enzyme-containing animal and plant tissues, have a clear or distinct enzyme catalytic activity, are effective in improving the digestion and absorption of feed nutrients by animals, and have biological evaluation criteria and performance requirements. They are enzyme products used as feed additives. 4 Classification, enzyme types and dosage forms of feed enzyme preparations 4. 1 Classification of enzyme preparations for feed
Enzyme preparations for feed can be divided into single enzyme preparations for feed, complex enzyme preparations for feed and mixed enzyme preparations for feed: 4.1.1 Products are separated and purified to contain only one functional enzyme component. Enzyme preparations for feed with the same composition and narrow range are called single enzyme preparations for feed. 4.1.2 Products containing two or more functional enzyme components - these enzymes are specific according to the different feed raw materials and system homogenization, and have enzyme catalysis on multiple components in the feed are called complex enzyme preparations for feed. 4.1.3 Products containing two or more functional enzyme components - these enzymes are specific according to the different feed raw materials and system homogenization, and have enzyme catalysis on multiple components in the feed are called complex enzyme preparations for feed. The feed enzyme preparation containing two or more main enzyme activities and having the same chemical reaction on some ingredients in the feed is called mixed feed preparation.
NY/T722—2003
4.2 Enzyme types of feed enzyme preparations
The main enzyme types and activities of feed enzyme preparations should be stated. The enzyme types that can be used as feed enzyme preparations should include the following categories: 4.2.1 Enzymes that strengthen animal endogenous digestive enzymes: such as acid protease, neutral protease, α-amylase, saccharifying enzyme, etc. 4.2.2 Enzymes that decompose non-starch polysaccharide anti-nutritional factors. Such as xylanase, β-glucanase, Ⅱ polysaccharide, etc. 4.2.3 Enzymes that destroy other anti-nutritional factors. Such as pectinase, phytase, etc. 4.3 Dosage forms of enzyme preparations for feed
Dosage forms of enzyme preparations for feed are divided into: solid dosage form and liquid dosage form. 5 Requirements
5.1 Safety requirements
5.1.1 If the source of enzyme preparations for feed comes from enzyme-containing animal or plant tissues, the enzyme preparations extracted from the edible parts of animals and plants are required to be used as the source of enzyme preparations for feed. The safety of the tissues should be determined: 5.1.2 If the source of enzyme preparations for feed comes from enzyme-producing microbial fermentation engineering, the microbial fermentation strain should be a special one. The enzyme-producing microbial fermentation strain that meets the following three conditions is a safe strain; the strain is non-pathogenic (including animal and plant pathogenicity); does not produce toxins and other harmful physiologically active substances; ... - the genetic stability of the bacteria is stable, easy to preserve, not easy to degenerate, and not easy to be infected by bacteriophages, etc. The fermentation strains that meet the requirements of food additives as enzyme sources can be judged as safe strains. 5.1.3 The process of producing enzyme sources through animal and plant tissue extraction and microbial fermentation engineering must be a safe process. According to the requirements of food grade preparations 1. Process 5.1.4 Any preparation extracted from the tissue of non-edible parts of animals and plants or produced by bacteria other than bacteria used as food additives shall be submitted to the statutory authority for safety testing according to the requirements of GR15193.1-1994. It shall not be used as the source of enzyme preparations for feed: It shall not be used as the source of enzyme preparations for feed using safe animal and plant tissues or non-safe strains. 5.1.51. Industrial grade Enzyme preparations are generally not allowed to be directly used in feed preparations. Only industrial preparations that meet the requirements of food additives can be used as the source of enzyme preparations for feed. It is strictly prohibited to use industrial enzyme preparations produced by ammonium sulfate precipitation process as the source of test reagents for feed.
5.1.6 During the post-treatment and re-production of enzyme sources, all raw materials, auxiliary materials, and additives in the process must be food grade or feed grade. 5.1.7 The entire production process must not be contaminated by pathogenic bacteria, pathogens, and other 5.1.8 Antibiotics, microorganisms and other prohibited drugs should not be added to the product. 5.1.9 For enzyme products, the enzyme source obtained by fermentation should state the strain of the enzyme and the basis for safety determination; for enzyme sources from animals and plants, the safety of animal and plant tissues should be stated; the production process should state whether it complies with the safety requirements of the standard. 5.1.10 The hygienic indicators of enzyme products for feed shall not exceed the standards specified in this standard. 5.2 Sensory indicators
a) Solid dosage form: consistent color, uniform particle size, no mold, deterioration, caking and peculiar smell; h) Liquid dosage form has a certain color and special smell of enzymes, the liquid is uniform, without precipitation and peculiar smell; 5.3 Physical and chemical indicators
a) Solid dosage form should be labeled with four indicators: enzyme activity, moisture, particle size, and mixing uniformity; h) Liquid dosage form should be labeled with four indicators: enzyme activity, pFI, and content; 5.4 Hygiene indicators
Should comply with the regulations in Table 1.
Monument (as As) (mg/kg)
Lead (as Pb)/(mg/kg)
Pot (as Ce)/(mg/kg)
Sand lemon fungus
Taiyu bacteria group/(individuals/1CO*)
Aflatoxin B/(ug/kg)）
Sanitary indicators
Not detectable
NY/T 722--2003
Jiang: If the sample is in liquid dosage form, the calculation results of tablets, lead and pot are expressed in gram (mg1); the calculation result of large coliform group is expressed in pieces per 100 ml (pieces/100 ml); the calculation result of aflatoxin B is expressed in microgram per plant (μg/L). The index values are the same as those in Table 1. Test methods
6.1 Sensory organs
Weigh 0g (or 50)ml of the sample), observe with the naked eye, smell and touch with hands. 6.2 Enzyme activity
Perform according to the agency standards.
6.3 Moisture content
According to GB/T 6435-1986:
6.4 Particle size
1986 implementation
According to GB/T 5917
6.5 Mixing uniformity
6.5.1 Determination method
Ten samples are taken from different parts of the same batch of products, with each sample size being 50 points. The distribution of the ten samples must take into account the representativeness of the depth, number of bags or material flow in all directions. Each sample must be taken out from one point. No turning or mixing is allowed before sampling. Each sample is fully mixed in the laboratory. Ten samples are taken from the sample by the quartering method, and the enzyme activity of the representative enzyme is measured. Then the coefficient of variation is calculated. 6.5.2 Calculation of the determination results
The average value is given by formula (1):
Standard deviation $Where (2) and (3) are given:
r + (ry) (r -
e+-r++ - 10
The coefficient of variation CV is given by formula (1):
Where:
Enzyme activity of each determination:
The mean value of enzyme activity;
Standard deviation.
NY/T 722—2003
6. 6 plI value
According to the determination method of pH value in QB/T 1803—19936. 7 Capacity
According to the determination method of bulk density in QB3/T1803-199. 6.8 Arsenic
According to GB/T130791999 Heat..
According to GB/T:3085-1991.
According to (13/T130821991.
6. 11 Salmonella
According to GB13-91
6.12 Coliform
1991.
According to GB/T4789.3-391 Pyrolysis
6.13 Aflatoxin B
According to GB/T13/T172801998
7 Inspection Rules
7.1 Batches
Products produced and packaged by cattle production plants in shifts of ten days with the same process conditions, product name, batch number and specifications are: batches
7.2 Sampling Method
Randomly take a single sample from the packaging of each batch of products: Use a clean and suitable sampling tool to reach into the depth of one-fourth of the sample to be taken, and use Take enough samples by cross-pollination method, mix the collected samples thoroughly, and divide them into 2 parts by quartering method. Put half of them into clean and dry sample bottles or sealed bags, and affix a label with the manufacturer's name, product name, batch number, sampling date, etc. Take samples from the same batch of products. The amount of each sample should not be less than eight times the amount of the sample required for inspection. The samples are put into sample bottles or sealed bags, one part is sent to the laboratory for inspection, and the other part is sealed and kept away from light for inspection. 7.3 Factory inspection
7.3.1 Factory inspection items
a) Solid dosage form: sensory indicators, clarity, moisture, particle size and packaging. b) Liquid dosage form: sensory indicators, activity, pH, content and packaging: 7. 3.2 Determination of the sample method
The sample taken shall be inspected according to the factory inspection items. If any index in the inspection result is unqualified, the sample shall be re-sampled for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still unqualified indexes in the re-inspection result, the whole batch of products shall be judged as unqualified and cannot be shipped out.
7.3.3 The batch of products shall be sent to the quality inspection department for factory inspection. Only after the inspection is qualified can the certificate of conformity be issued and shipped out. 7.4 Type inspection
Type inspection items: All items in Chapter 5 sensory indicators, physical and chemical indicators, and hygiene indicators. Type inspection should be carried out in the following cases: 7.4.21 When issuing the production license product approval number: b) When the statutory quality inspection department makes a request: When there are major changes in raw and auxiliary materials, process and main equipment; ) When resuming production after stopping for a period of time,
NY/T722-2003
7.4.3 Judgment method: The sampled samples shall be inspected according to the type inspection items. If any item in the inspection result is unqualified, the sample shall be collected again for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still points of unqualified in the re-inspection result, it shall be classified as unqualified.
8 Marking, labeling, packaging, transportation and storage 8.1 Marking
Product The outer packaging of the product must indicate: product name, specifications, trademark, cattle manufacturer, etc. and use a clear feed label and inspection certificate. 8.2 Label
Product labeling shall be implemented in accordance with GB10648
8.3 Packaging
Product packaging must be: airtight, waterproof, light-proof, and firm. 84 Transportation
The product contains biologically active substances, and light, temperature, and humidity can easily cause fire. The product must be covered during transportation to avoid exposure to the sun, rain, and heat. It must be handled with care and must not be mixed with toxic, harmful, or other polluting items. 8.5 Storage
The product must be stored in a cool, dry, light-proof place. The storage warehouse must be kept clean, cool, ventilated, and protected from heat and moisture. 722-2003
Agriculture of the People's Republic of China
Industry Standard
Test for Enzyme Preparations for Feed
NY 722-: 2003
Diwei Standard Publishing House
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal Code: 100)46
Tel: 6552394688517548
China Standard Publishing House Suhuangdao Printing Co., Ltd. Guangzhan Printing Co., Ltd. Xinhua Bookstore Beijing Distribution Office
Announcement by Xinhua Bookstores in various places
F880×123C 1/=6
Sheet 3/1 Number of words 12 F words
First edition in February 2011 First printing in February 2004 Printing efficiency 1—600
$ number: :56066 - 2-15356
Price 10.COT
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Report phone number: (010)68533533
20022 1/2. Determination of the sample method
The sample taken shall be inspected according to the factory inspection items. If any index in the inspection result is unqualified, the sample shall be re-sampled for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still unqualified indexes in the re-inspection result, the whole batch of products shall be judged as unqualified and cannot be shipped out.
7.3.3 The batch of products shall be sent to the quality inspection department for factory inspection. Only after the inspection is qualified can the certificate of conformity be issued and shipped out. 7.4 Type inspection
Type inspection items: All items in Chapter 5 sensory indicators, physical and chemical indicators, and hygiene indicators. Type inspection should be carried out in the following cases: 7.4.21 When issuing the production license product approval number: b) When the statutory quality inspection department makes a request: When there are major changes in raw and auxiliary materials, process and main equipment; ) When resuming production after stopping for a period of time,
NY/T722-2003
7.4.3 Judgment method: The sampled samples shall be inspected according to the type inspection items. If any item in the inspection result is unqualified, the sample shall be collected again for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still points of unqualified in the re-inspection result, it shall be classified as unqualified.
8 Marking, labeling, packaging, transportation and storage 8.1 Marking
Product The outer packaging of the product must indicate: product name, specifications, trademark, cattle manufacturer, etc. and use a clear feed label and inspection certificate. 8.2 Label
Product labeling shall be implemented in accordance with GB10648
8.3 Packaging
Product packaging must be: airtight, waterproof, light-proof, and firm. 84 Transportation
The product contains biologically active substances, and light, temperature, and humidity can easily cause fire. The product must be covered during transportation to avoid exposure to the sun, rain, and heat. It must be handled with care and must not be mixed with toxic, harmful, or other polluting items. 8.5 Storage
The product must be stored in a cool, dry, light-proof place. The storage warehouse must be kept clean, cool, ventilated, and protected from heat and moisture. 722-2003
Agriculture of the People's Republic of China
Industry Standard
Test for Enzyme Preparations for Feed
NY 722-: 2003
Diwei Standard Publishing House
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal Code: 100)46
Tel: 6552394688517548
China Standard Publishing House Suhuangdao Printing Co., Ltd. Guangzhan Printing Co., Ltd. Xinhua Bookstore Beijing Distribution Office
Announcement by Xinhua Bookstores in various places
F880×123C 1/=6
Sheet 3/1 Number of words 12 F words
First edition in February 2011 First printing in February 2004 Printing efficiency 1—600
$ number: :56066 - 2-15356
Price 10.COT
Website bzcbs. con
Copyright reserved
Slow infringement will be investigated
Report phone number: (010)68533533
20022 1/2. Determination of the sample method
The sample taken shall be inspected according to the factory inspection items. If any index in the inspection result is unqualified, the sample shall be re-sampled for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still unqualified indexes in the re-inspection result, the whole batch of products shall be judged as unqualified and cannot be shipped out.
7.3.3 The batch of products shall be sent to the quality inspection department for factory inspection. Only after the inspection is qualified can the certificate of conformity be issued and shipped out. 7.4 Type inspection
Type inspection items: All items in Chapter 5 sensory indicators, physical and chemical indicators, and hygiene indicators. Type inspection should be carried out in the following cases: 7.4.21 When issuing the production license product approval number: b) When the statutory quality inspection department makes a request: When there are major changes in raw and auxiliary materials, process and main equipment; ) When resuming production after stopping for a period of time,
NY/T722-2003
7.4.3 Judgment method: The sampled samples shall be inspected according to the type inspection items. If any item in the inspection result is unqualified, the sample shall be collected again for re-inspection. The sampling range or sample quantity shall be twice that of the first time. If there are still points of unqualified in the re-inspection result, it shall be classified as unqualified.
8 Marking, labeling, packaging, transportation and storage 8.1 Marking
Product The outer packaging of the product must indicate: product name, specifications, trademark, cattle manufacturer, etc. and use a clear feed label and inspection certificate. 8.2 Label
Product labeling shall be implemented in accordance with GB10648
8.3 Packaging
Product packaging must be: airtight, waterproof, light-proof, and firm. 84 Transportation
The product contains biologically active substances, and light, temperature, and humidity can easily cause fire. The product must be covered during transportation to avoid exposure to the sun, rain, and heat. It must be handled with care and must not be mixed with toxic, harmful, or other polluting items. 8.5 Storage
The product must be stored in a cool, dry, light-proof place. The storage warehouse must be kept clean, cool, ventilated, and protected from heat and moisture. 722-2003
Agriculture of the People's Republic of China
Industry Standard
Test for Enzyme Preparations for Feedwww.bzxz.net
NY 722-: 2003
Diwei Standard Publishing House
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal Code: 100)46
Tel: 6552394688517548
China Standard Publishing House Suhuangdao Printing Co., Ltd. Guangzhan Printing Co., Ltd. Xinhua Bookstore Beijing Distribution Office
Announcement by Xinhua Bookstores in various places
F880×123C 1/=6
Sheet 3/1 Number of words 12 F words
First edition in February 2011 First printing in February 2004 Printing efficiency 1—600
$ number: :56066 - 2-15356
Price 10.COT
Website bzcbs. con
Copyright reserved
Slow infringement will be investigated
Report phone number: (010)68533533
20022 1/
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