This standard specifies the determination method for the residues of permethrin in plant-derived foods. This standard is applicable to the determination of permethrin residues in grains, vegetables and fruits. GB/T 5009.106-2003 Determination of Permethrin Residues in Plant-derived Foods GB/T5009.106-2003 Standard download decompression password: www.bzxz.net

ECS 67.040
National Standard of the People's Republic of China
GB/T5009.106—2003
Generated from GA14879—1991
Determination of permethrin residnes in vegelahle finds2003-08-11Published
Ministry of Health of the People's Republic of China
Standardization Administration of the People's Republic of China
Implemented on 2004-01-01
G 5009.106—2003
This standard replaces GB14879:1994 Method for determination of dichloroisocyanurate in food. Compared with GB1487—1991, the main changes of this standard are as follows: the Chinese name of the standard is changed, the Chinese name of the standard is changed, and the structure of the original standard is modified according to the GB/T20001.4—20013 standard part 3, chemical analysis method.
The standard is proposed by the Ministry of Health of the People's Republic of China. The drafting units of this standard are: Beijing Agricultural University, Beijing Academy of Agriculture and Forestry Sciences, Institute of Agricultural Sciences, Ministry of Health, National Health and Family Planning Commission, and the main starting points of the standard are: Mingjiu, Zhengji, Guoluo, and Shijie. The original standard was issued in 2003 and this is the first revision. 28www.bzxz.net
1 Scope
GB/T5009.106—2003
Determination of the content of chlorpyrifos in plant foods This standard specifies the determination method of chlorpyrifos residues in plant foods. This standard is applicable to the determination of chlorpyrifos transesterification residues in palm food, banana vegetables and fruits. 2 Principle
Electron capture detector has high sensitivity to electronegative compounds. In the sample, these compounds are extracted and purified, and then detected by gas chromatography electron capture detector. The peak height (area) of the sample is compared with the peak (area) of the standard sample. The equivalent content of the sample is calculated.
3 Test
3.2 Petroleum ether.
3.3 Ethyl acetate.
3.4 ​​Caustic soda.
3.5 Sodium sulfate,
3.6 Flour soil (60 mesh ~ 80), activated at 130℃ for 24h before use, stored in a desiccator. 3.7 Dimethoate standard solution, accurately weigh the chloranthone standard, extract with methyl alcohol or stir, and store in ice. 3.8 Dimethoate solution: Decompose the solution before use, and store in the refrigerator for later use. 4 Collection
4.1 Gas chromatograph, with NiFCI),
4.2 Tissue extractor
4.3 Sample extractor
4.4 Rotary extractor:
4.5 Chromatographic column. 1m (inner diameter) × 17cm
5 Analysis steps
5.1 Uptake
5.1.! For fruits and vegetables, weigh 10 g of the chopped fruits and put them into a tissue crusher, add 30 ml of the mixture and extract with a Buchner funnel. Rinse the residue with a small amount of acetone, collect all the liquid in a 500 mL separatory funnel, add 200 ml of 20/T sodium chloride, extract with petroleum ether (0.25, 25 ml) three times, pass the petroleum ether layer through anhydrous sulfur film, and concentrate with a rotary evaporator to 1 m2-3 mL for purification.
5.1.2 Cereals: Weigh 20g of crushed sample, put it into filter paper, extract it with 100mL acetone + 1:1 mixture of aldehyde in a chromatographic extractor for 45min, then transfer it to neutralizer for 5min. The following steps are the same as for fruits and vegetables: 5.2 Purification
Put a little defatted liquid (extracted with 25mL petroleum fermentation mixture) on the bottom of the chromatographic column, then put it on top, add 2g sodium sulfate, add 4g silica gel, and then add 10mL anhydrous sodium sulfate on the top, shake it slightly to make it full, dissolve it with 10L 2% or 5% ethyl acetate petroleum aldehyde, discard the liquid, put the concentrated sample extract into the column, wash it with 70ml of the above solvent, collect 29
GB/T 5009.106—2003
All eluents were condensed and settled for gas chromatograph analysis: 5.3 Chromatographic recordings
5.3.1 Chromatographic analysis: 3mm light) x.5m, internal micron 8% V-1 (1+3% Aigs/aChrQ5.3.2 Photoemission 886.
5.3.3 Inlet temperature: 240,
5.3.4 Detector temperature: 255.
5.3 .5 Air flow velocity: 71mt./min5.4 Fixed
According to the night instrument, a series of standard concentrations of various standards are prepared, and the peak quotient of each standard is obtained. The peak quotient of different oxygen-containing auxiliary lipids is measured. Take the sample liquid-the standard liquid with high concentration is compared with the high concentration of the filtered liquid, and calculate its relative purity.
5,5 Calculation
Calculate according to the following formula:
hc, Q.
1.1 Drug content in the sample, unit is meter (mm);
standard drop rate: single point per (m/s); standard dissolution rate: unit is dispersed volume (mm); standard peak separation: single point per (mm); sample weight: unit is gram (g); Q
sample weight: unit is liter). 6 Precision
under the condition of good accuracy, the value of the single determination shall not exceed the average value of the sample.
color of the dihydrogen chrysanthemum acid
color of the dihydrogen chrysanthemum acid
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