Carbendazim,thiram and carbofuran suspension concentrates for seed dressing
Some standard content:
NS 65.020
Agricultural Industry Standard of the People's Republic of China
NY 621—2002
Duofu Ke suspension seed dressing agent
Carhendazim, thiram and carhofuransuspension concentrates for seed dressing2002-12-30Issued
Implementation on 2003-03-01
Issued by the Ministry of Agriculture of the People's Republic of China
Chapter 3 and Chapter 5 of this standard are mandatory, and the rest are recommended. NY621-2002
This standard is prepared in accordance with the requirements of GB/117763
31999 seed coating agent product standard room specification" and formulated in combination with the actual situation of domestic production enterprises
This standard is issued by the Agricultural and Economic Information Department of the Ministry of Agriculture. This standard is under the jurisdiction of the Institute of Drug Control of the Ministry of Agriculture, and the technical standard is issued by the Agricultural Pesticide Cultivation Institute. This standard is issued by the Chinese people Che Guoping, Sun Wengtu, and Qiulan. This standard is entrusted to the Ministry of Agriculture Pesticide Inspection Institute for interpretation. The common name, structure and basic physical and chemical formula of the active ingredients in the product of Duofu Ke Suspension Seed Coating Agent are as follows: a) Polybacterium
IS Common name cArhendazim
Chemical name: N-benzimidazole-2-ylcarbamate Structural formula:wwW.bzxz.Net
Empirical formula C, H, NO
Relative mass: 191.2 (according to the 1997 International Relative Atomic Mass Calculator) Biological activity: decomposition point at 310℃]
vapor k(2C): 100Fa
NY621-2002
Decomposition 20)/): 84 in water) 78) C. Chloromethane 68 in chlorine 10n
Quality: Stable to acid, relatively stable to light and heat, decomposes slowly under warm conditions 1) Thiram
ISU wine name: tlhitem
Chemical name: bis(N,N-methylformamide)-compound structural formula:
(CI,NCSSNCH.),
actual formula NS
decomposition mass: 2.4 according to the 337 year letter International relative atomic mass) Biological activity: melting point, 145 ° C Solubility (21:3//1): 0.018 in water, a0 in chlorine, 280 in ethanol less than 10 Stability: easy to decompose after melting, long-term storage in hot and mixed environments) g/ml Common name: carbolurEn Chemical name, 2,3-dihydro-2,3-dibenzo-7-yl N-methylamino ester Structure! OCNHCH VY621-2002 Empirical formula: .H.NO Relative molecular mass: 221.26 (connection: 1937 International Relative Mass Calculation) Biological correction: soft and easy to decompose. 150℃~152℃
Gentlemanometer (33℃): 2.1mP:
Distinction (20)/(mg/L): 320 in water, 20g isocyanate in diisocyanate 25-.30 stability; the stability and neutrality will be observed, and the reducing medium is not guaranteed. 1 Scope
This standard does not specify many requirements, test methods and marking labels, packaging, printing, storage and transportation of floating agents. This standard applies to each type of floating agent. 2 Normative references
The following documents become the terms of this standard through reference. From the date of application of the document, all subsequent amendments (excluding delayed proposals) or revisions are applicable to this standard. However, parties to an agreement based on this standard are encouraged to study and use the latest versions of these documents. For any document without an F date, its new version shall apply the following standard: GB/T169-2 Method for determining μH value of pesticides G/T1634-2 Product acceptance criteria for pesticides G13/1635 Method for sampling from commercial enterprises GB3795 General rules for agricultural packaging GB/T16.50 Pesticide sales agent, method for determining fineness of wettable powder 3 Requirements 3.1 Composition and external composition: This product is composed of polyvinyl alcohol, polyvinyl alcohol, polyvinyl alcohol or original medicine and other additives (including colorants): It should be movable and evenly distributed with the base. It may have a small amount of precipitation after long-term storage, but it should be able to return to its original state when touched or tested by hand at room temperature, and there should be no precipitation.
3. 2 M·F·K-series seed dressing agent shall meet the requirements of Table 1 Table 1 Multi·F·K-series suspension seed dressing control items Index items
Total content of effective products/(number
H multi-content: ()
Fu-type double-containing other)
Chongbai double-benefit/%）
Monthly ocean (%)
Separation (passing 44μm acid sieve) (%)
Degree range 25uas)
Sensitivity to film
Included in average degree%）
Included in dressing rate (
Indicated content:
Indicated quantity
Indicated content
Indicated content·
a. 3~7. (
will be qualitative
thermal cost specification
. Indicate the content is too high and 0.00%
in normal production, a test is conducted every month, 4 test methods
4.1 Sample
Table 1 continued)
NY621-2002
according to the "commercial pesticide collection method" in /T10, the packaging of the oil list is determined by the random number table method, and the final sampling is not less than 250mL,
4.2 Identification test
commercial liquid chromatography method
vehicle identification test can be carried out after the effective ingredient content is measured. Under the color operation conditions of the scanning room, the color of the sample liquid is measured by the soil bee The relative difference between the retention time of bacillus-methyl and the retention time of the color change of thiram or chloranil in the standard sample should be within 1.5 hours. When the specified test method is doubtful for the identification of the active ingredient, at least one other method should be used for retrograde identification. 4.3 Determination of the content of thiram, chloranil and chloranil 4.3.1 Summary of the method The sample is decomposed in methanol and the new sample is scanned with decahydroacetic acid + water as the main chromatographic medium. The sample is fixed with a stainless steel column and filled with a chain. The sample is separated and determined by high-performance liquid chromatography and quantified by the external standard method. 4.3.2 Reagents: isocyanate, water, hot water, ice water, ammonia, bacterium standard: known content, 0.0%, Fuguan effective standard: 9.0% chloramphenicol. 4.3.3 Receiver: dynamic chromatography, variable liquid ultraviolet detector, chromatograph: 25T×.6mm stainless steel, inner Table 4 Specifications for LLT1M reverse phase chromatography column: 5Tm (or other reverse phase chromatography column with the same column efficiency) Guard column: Temperature accuracy; Chromatographic data processor: Microinjector ... Ultrasonic washer. 4.3.4 High performance quasi-phase chromatography operating conditions Phase isopropyl acetate + water + water = 2 + F1.1 (volume ratio): Flow rate: 3.7 mL/min Measured liquid length: 2795.m Sample maximum: NI621-2002 Temperature: 35°C Retention time: Carbendazim 5.5min. 5.-3min, 7.1min. The reverse operation parameters are typical (see Figure 1). The characteristics of different instruments can be taken into account and appropriate adjustments can be made for the given dyeing parameters in order to obtain the best effect.
Multi seedlings,
2—— bundles;
3—— gram gate.
Figure 1 High-performance chromatography of polyoxyethylene sulfone suspension seed dressing agent 4.3.5 Determination steps
4.3.5.1 Preparation of standard samples
Weigh the carbendazim standard sample (accurate to C1g), thiram and budweiser standard samples (accurate to C1g) according to the ratio of the effective ingredients in the sample, and determine the accuracy to C1.) in the same 10T volumetric flask, add 1 mL of ethyl acetate, and use a ultrasonic washing machine to ultrasonically treat the standard sample. Add 9 mL of methanol, use a ultrasonic washing machine to ultrasonically separate the effective ingredients, take out and bring to room temperature, and then use methanol to hold the sample for later use. 4.3.5.2 Sample drop roll preparation
Mix the sample thoroughly without color and take 30 mg of doxorubicin accurately to 0.100 ml of the sample was placed in a bottle with 2 mL of glacial acetic acid and treated by ultrasonic cleaning for 2 min. Then 100 ml of methanol was added and the solution was effectively separated by ultrasonic cleaning. After taking it out and letting it cool to room temperature, it was fixed to volume with methanol. The separated liquid was transferred to a 10 ml centrifuge tube and then? [Trnin speed sound heart to dissolve wave avoidance, standby, 4. 3. 5. 3 Determination
Under the above dial recording fiber, wait for the instrument base level to be determined, reverse the number of needles of standard sample liquid, until the adjacent two needles of the bacteria record (Fu Guan Shuang Jing Bai Dai> sleep face proposed change is less than!, when 5%, according to the standard sample rate change, test interception drop effect, sample drop, standard sample drop sequence. 4.3.6 Calculation period
The measured two needles of sample solution and the two needles of standard sample solution before and after the sample are respectively averaged, and the content of carbendazim (Fu Guan Shuang, Ke Bai Jian) in the sample is expressed as a mass fraction X, (). (1) Calculation: X = -A ×m×P
A, the average peak area of chlorpyrifos and chlorpyrifos in the standard sample, A. The average peak area of chlorpyrifos (chlorpyrifos, chlorpyrifos or) in the standard sample, in milligrams (mg: the mass of the sample before hanging, in grams [
P-the fraction of chlorpyrifos and chlorpyrifos in the standard sample, %, 4.3.7 Allowable difference
The difference between the results of two and a half tests is not less than 5 times the arithmetic mean and the test results are carefully calculated and measured. 4
4.4 Determination of resistance
Carry out according to G/T 1501.
4.5 Determination of maximum floating rate
4.5.1 Summary of the method
NY 621-2002
Use standard water to prepare the test sample into a liquid of appropriate concentration. Under the specified conditions, place the sample in a humid water bath at 0 m below the surface of the water and collect the amount of the substance floating in the liquid, and calculate the floating amount. 4.5.2 Reagents
Anhydrous calcium sulfate;
Sedimentary ethanol
Hydrogenated magnesium sulfate (six crystal water);
Standard hard water preparation: Take anhydrous calcium sulfate, 304 days and iron oxide with six crystal water. 0.139g. Dilute with steamed stuffing water to L.
4.5.3 Only instruments
Beaker:
Only measuring tube; 250ml. Inner diameter a8.5mr~4mmoml~250ml shaving space 20.ccm~21.cm, 250l scale line and short capsule bottom surface distance cm6cm
Glass suction: glass milk end and corresponding suction device aligned; constant source water bath: 3
Water tray: 890
4, 5. 4 Remaining steps
Weigh 5 g each of samples A and B accurately to 0.02 g with a difference of less than C. 1) Put 50 mL of 301 standard hard water into each beaker. Use F to make a circular motion at a speed of 12 cm/min for 2 min. Transfer the remaining contents in the beaker to a measuring cylinder with 30=1 mL of standard hard water. Dilute the sample with 30℃ standard hard water at a rate of 1/4 of the scale. With the bottom of the measuring cylinder as the axis, turn the measuring cylinder up and down 30 times within 1 min (put it back on the bed once, each time for about 2 min).
Sample A is immediately washed with a vacuum cleaner within 108~15 min. 9/10 of the contents, i.e. 225 m! Remove the residue by shaking, making sure that the opening of the pipette is always below the surface. Transfer the remaining 2 mL of the residue to a 10 mL beaker that has been dried to constant weight. Add 1 mL of water at 1°C to 90°C, add 1 mL of ice-free ethanol, and continue to remove water in a water bath until the constant weight reaches 0.002 mL). The original amount of the residue is mg-
. Place the sample vertically in a test tube. After 50 min, use a pipette to remove 9/10 of the residue, i.e. 225 mL. Remove the sediment in the sample by shaking or removing the amount of sediment, making sure that the opening of the pipette is always below the surface. 25 mL of the residue is left. Reduce the residue to a sample and take the mass of the residue m. 4.5.5 Calculate the total mass x of the sample. () Calculate according to formula (3): X: =(12m=×12×100
Wherein:
m, the mass of dry matter in the 25mL residue at the bottom of the container, in grams (g); mL——the mass of the substance in the 25mL residue at the bottom of the container: in grams (g). 4.6 Determination by sieve analysis
GB/16150) is a reverse sieve method.
4.7 Determination of viscosity
4.7.1 Summary of the method
NY6212002
Use a digital frequency conversion grid to select suitable characteristics. At a speed of 3rmin, measure the viscosity of the sample. 4.7.2 Instrument
Digital rotary viscometer:
Rotation number:
Constant pan water content: 25±1t:
4.7.3 Determination steps| |tt||After the sample is squeezed, keep it in a constant temperature bath. When the sample temperature reaches the required value, dry the sample and place it in a constant temperature water bath for 25 h. Adjust the viscosity. Install the rotor and rotate the rotor continuously until the rotor reaches (3C1) r/mn. Slowly insert the rotor into the sample so that the surface is not concave on the rotor. Start the test machine and read the viscosity (P·s) 4.8 after 1 min. Determination of the quality| |tt||4.B.1 Method Summary
Collect a certain amount of test batch of culture medium, stir the culture medium to mix the sample and seeds thoroughly. Take the culture medium and observe the burning condition in the source for a certain period of time.
4.8.2 Reagents and Materials
Cultivation medium: diameter about 200mm, depth 30t;
Injection vessel 5ml, 1
± rice seed; 100% effective egg white is ?8 1. The moisture content is between 2% and 14%. The environmental conditions of the harvesting room are: temperature is 20-30%, relative humidity is 40%-69%, 4.8.3 The test frequency
Take the main seed and keep it in the incubator. Use a syringe to absorb the sample. Inject it into the incubator, add 5 inches of rotation. Open the seed and spread the seed coating flat to form a film. After 2) minutes, use a breaking stick to gently push the seeds. Check the surface of the seeds. If the seed coating on the surface of all seeds has solidified, the film is considered as a solid. 4.9 Determination of coating uniformity
4.9,1. Method summary
Take a certain number of coated seeds and seeds, respectively, and use:-Quantitative flash alcohol culture solution to take the measured light absorption rate, calculate the test value of coating uniformity,
4.9.2 Reagents and apparatus
Good quality% ethylene!
Z alcohol solution 9%H=
Microscope: 5mlL;
Accurate commercial center:
Spectrophotometer:
Colorimetric, 1m:
4.9.3 Determination of oxygen
Measure the absorption of 100 qualified coated tablets, divide them into ten and store them in 4 commercial tubes, use 2.mL~5.Um (within the linear range of absorbance) ethanol solution, add 1% ethanol solution, soak for 15min, take the sample without soaking, let it stand or drain, get the clear solution, take the wave as reference, and determine its absorbance A at the maximum absorption wavelength (5531 with the detection time as the dyeing time, and its local component as the rate, and its component can be selected as the report period) 4. 59. 4 Calculation of shrinkage
Arrange the 5 measured absorbance values from small to large: and calculate the average absorbance value 4: Test column coating average viscosity x (treatment> connect formula (3) calculation constant:
In the formula:
×100=4m
-Absorbance should be between 0.74~.3A, centrifugal sound within the range,- total centrifugal pressure.
4.10 Determination of package shedding
4.10.1 Summary of the method
NY621—2002
You Take a certain amount of coated seeds, shake them on a shaker for a certain time, and then filter the seeds with ethanol to determine the absorbance and calculate the shedding rate.
4.10.2 Receiver and reagents
Electrical flask: 25mL;
Spectrophotometer:
Shaker, 20r/min. HY-2A speed-adjustable multi-function device: 95% CH 2 O (95% ethanol): 55:45 HO
4.10.3 Experimental steps
Weigh! (accurate to C.52) Two portions of coated seeds with qualified abdominal properties are placed in dry bottles. One portion is accurately added with 195 ml of ethanol, and then stoppered and soaked for 1 hour. Place in an ultrasonic cleaner and vibrate for 10 minutes to fully dissolve the seed coating agent on the surface of the seeds. Take out the seed coating agent and place it in the preheater for 20 minutes. Collect the seed coating agent and drain it for 10 minutes.0L is dissolved in a 50mL weighing bottle until the required amount of solution is required. The other portion is placed on a dropper: After 11[riⅡ, carefully transfer the seeds to another triangular flask and treat according to the method of 4 to obtain solution B. Filter with alcohol as the reference and determine its absorbance at the maximum temperature (550 nm). Use luminol as the detection wavelength for dyeing. If it is a dye, it can be selected according to its composition. 4.10.4 Calculation of coating shedding rate X (%) is calculated according to formula (4): As /m A m × 1cc m Am Am × 10 A In the formula: A is the mass of the coated seeds weighed, in grams. L is the mass of the prepared solution weighed, in grams (R)! A,——absorbance of A of the sample
At——absorbance of the sample.
4.10.5 The difference between the results of the two tests is allowed to be less than 1%, and the average value is taken as the test result. 4.11 Low temperature qualitative test
4.11.1 Method summary
The sample is kept at 0℃ for 1 hour, and the appearance is observed for any changes. Continue to store at 0℃, and test its physical and chemical indicators. 4.11.2 Refrigerator
Refrigerator: Maintain ℃,
One bottle: 100 ml.n
4.11.3 Test steps
Take 80mL of the sample and place it in the other bottle, keep it at 9℃±1℃ for 1 hour, stir it every 15min, observe its appearance for 15min, and continue to place it under the above conditions? After 71, take the flask out of the mountain, return to the temperature, and measure the adhesion and analysis of NY621--2002. The test results meet the standard requirements and are qualified. 4.12 Hot storage blanket qualitative test 4.12.1 Constant temperature box (or constant temperature water bath) 4:2 Amway bottle (or sealed glass bottle that can still be judged at 54℃): 50mL medical syringe, 5)mI 4.12.2 Test steps Use an injection timer to inject about 1 Pour the sample into a clean ampoule (avoid the sample contacting the bottle neck), seal it with a high temperature fire (avoid the volatilization of the reagent. At least 3 bottles are divided. Place the sealed ampoule in a container, and then place it in a constant temperature box (or constant temperature water bath) at 54°C for 14 hours. Take it out and divide the ampoule into sections. The samples with different quality and changes are tested for gram, polysorbate and related floating contents within 24°C. The test results show that the relative percentages of polysorbate, polysorbate and gram floating contents are all less than %, and the floating rate is not lower than 1%. 0. Research for the purpose of combination, 4.13 Product performance inspection and acceptance
The product and acceptance shall comply with the relevant provisions of (TS/T1634. Limit value processing, use the approximate value comparison method, 5 Marking, comprehensive labeling, packaging, storage and delivery
5.1 Rate·width·gram floating tax clothing marking, the whole village packaging, shall comply with the relevant provisions of (B3796, 5.2 Multiple·phase·gram floating tax clothing packaging shall be calculated in units of 10hgkg=0kg.1cukg. Other forms can also be used according to user requirements or product conditions. The packaging should meet the requirements of 36. 5.3 The packages should be stored in a ventilated warehouse with stairs. 5.4 During transportation, it should be kept away from moisture and sunlight. It should not be mixed with food, seeds, and other materials. It should avoid contact with skin and dirty things and prevent inhalation through the mouth and nose. 5.5 In addition to the corresponding toxicity signs in the instructions or packaging materials, there should also be toxicity instructions, poisoning symptoms, detoxification methods and accidental damage. If ingested by mistake, atropine can be used for detoxification. 5.6 Under the specified transportation conditions, the shelf life of the seed coating agent is 2 years from the date of production.
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