GB/T 5009.37-2003 Analytical methods for edible vegetable oil hygiene standards
Some standard content:
ICS67.040
National Standard of the People's Republic of China
GB/T5009.37——2003
Generation G13/5004.47—199 catties
Method for analysis of hygienic standards of edible vegetable oils Issued on August 11, 2003
Ministry of Industry and Information Technology of the People's Republic of China
National Standardization Administration of China
Implementation on January 1, 2004
GB/T5009.37—2003
This standard replaces GB/T5009.37—1996 Hygienic standard for edible vegetable oils - Distribution method. Compared with GB/T6099.37-1996, this standard has the following improvements: - The colorimetric method for determination of peroxide value is added as the second method; - The structure of the original standard is revised in accordance with GB/T6001.4—2101 standard part 3: Chemical analysis methods; - The determination method of base value is revised. This standard is proposed and managed by the Ministry of Health of the People's Republic of China. This standard is approved by Shanghai Municipal Health and Epidemic Prevention Station, Tianjin Municipal Health and Epidemic Prevention Station, Anhui Provincial Health and Epidemic Prevention Station, Shaanxi Shangwei Niuhu Station, Liaoning Provincial Health and Epidemic Prevention Station, Hunan Provincial Health and Epidemic Prevention Station, and the Ministry of Health Food Hygiene Inspection and Testing Institute. This standard was first issued in 1985, and was first revised in 1996. This is the first revision. 34
1 Scope
Analysis method of edible vegetable oil hygienic standard This standard specifies the standard analysis method of edible vegetable oil. The standard is applicable to the analysis of edible vegetable oil hygienic standard GB/T5009.37--20Q3
The control limit of this prescription is n.10k/kg+ peroxide value and the second limit is 0.cE3mea/kg. 2 Normative references
The following documents are quoted in this standard and become the terms of this standard. For any cited document with a date, all subsequent amendments (excluding the contents of the survey) or versions are not applicable to this standard. However, the parties to the agreement on whether the latest version of the document can be used shall study whether it can be used as the latest version of this document. For a cited document with a date, the latest version applies to this standard. GB/T5009.11 Determination of total and inorganic toxins in food GB/T5009.22 Determination of xanthotoxin T in food GE/T3009.27 Determination of hydroxytoxin (R) in food B/T5009.188 Determination of hydroxytoxin in food
3 Sensory inspection
3.1 Color
Specification: cup end point 0mm, cup height 10mm.
3. 1. 2 Analysis Steps
Place the sample in a beaker at a height of not less than 1.5 m. First, place the sample under natural light in a room temperature, then measure its reflected light. Observe and describe the color according to the following: white, off-white, lemon yellow, yellowish yellow, orange, yellowish yellow, brown, brownish red, brownish brown, etc.
3.2 Odor and Taste
Place the sample in a beaker at 1.5 m. Heat it at 0.5 m. Stir it rapidly to remove the odor. Take a small amount of the sample to measure its taste and describe it according to the following: sweet, burnt, sour, bitter, spicy, etc. 4 Physical and Chemical Test
4.7 Fusion Valence
4.1.1 Principle
The separated fatty acids in plant oil are marked with potassium hydroxide. The amount of potassium hydroxide consumed per gram of plant oil is the aldehyde value. 4.1. 2 Reagents
4.1.2.1 Acetaldehyde-ethanol mixture: Mix acetaldehyde-ethanol (21> slurry. Use potassium emulsifier 3%/L) to neutralize until phenol indicator drops.
4.1.2.2 Hydroxide standard titration solution (e(K2O3)H2O) = c.350mn./T.4.1.2.3 Acid indicator: 10%/acetaldehyde solution.4.3 Steps
Weigh Take 0.8-10% of the mixed ethyl acetate-ethanol mixture from the subgastric cone, and dissolve it with oil. If necessary, put it in hot water to warm it up to promote its dissolution. Cool it to room temperature, add one drop of 2% indicator liquid, and titrate the molten salt with hydrogen peroxide (0.1%/drop). The end point is 4.1.4. Calculate the result
the sample is the price formula (1 reverse calculation
the cool price (in terms of ammonia oxidation) single step mg per point (/) method sample sample chemical evaluation standard who moistened the liquid rest right, single this is the toxicity (!: potassium hydroxide standard full determination of the actual concentration, the total is the shore oxygen small (mol/I.! -- the sample is a single surprise grams:
Ning 1nT potassium hydroxide standard tube liquid -30m when * hydroxide injection full grams of calculation followed by the guarantee of the two books use effective numbers,
4.1.5 beat density
in the year basic miscellaneous work quickly obtained twice through the text of the conclusion The absolute difference shall not exceed 10% of the arithmetic mean. 4.2 Peroxide value
4.2.1 Method I Titration method
4.2.1.1 Principle
The oil is freshly produced by the titration method. The actual chemical reaction is carried out by agreement with the actual chemical reagents to generate the sulphur dioxide. The calculation is as follows: 4.2.1.2 Reagents
4.2.1.2.1 Weigh the chemical reagents and weigh the droplets. Add 10% water and decompose it. Heat the acid to dissolve it. After cooling, add a brown bottle containing concentrated glacial acetic acid: 4.2.1.2.2
4.2.1.2.3 Standard titration method for sodium sulphate [eNa.S(.)=0.c23r10./[]. 4.2.1.2.4 Potential indicator (1c) Weigh 0.50% reactive sediment, a little wood. Reduce the paste, pour 59mL sand into the empty filter bottle: Filter: Before use:
4. 2. 1.3 Analysis steps
Weigh 200, 250 ml of the mud or sample to be filtered, mix with 5m methane aldehyde and completely pour it into the bottle, add 1.CerT. saturated screen solvent, filter, tightly cover the bottle, and shake gently for .min, then incubate for 3min. 100ml water is collected and titrated with sodium thiosulfate standard (0.002 0 0./L) and two fixed car Yan Dian Ren attached, 11 starch indicator solution, the blue disappearance of the fixed car is the road point, the same amount of trichloroacetic acid is reduced in glacial acetic acid, potassium hydroxide is reduced, water, the method of the time, the number of doses tested is 4.2.1.4 Calculation of shrinkage
formula or 2) and formula (to calculate. V) X...126D100
: .. X 2 78.8
, ... the unit of oxidation state is 10
, position
:--Trial discharge sodium tromethamine standard full set drop point reporting volume: single check for empty (m) let the reagent room pass the test sodium dropping point drop technical volume, single low light factory: representative of the standard Sabo method, the unit is mul/liter (mul/): sample page amount, the unit is gram ():
CB/T5099.37—2003||tt ||0.12h9——The mass of iodine that is eliminated with 1.nn1. sodium carbosulfate standard oil c (NerS.O.) = 1.0 mnl/L], unit is point ():
78.S--Two calculation factors
One calculation is retained as the empty effective number
4.2.1.5 Precision
The absolute difference between two independent determinations obtained under the same conditions should not exceed 10% of the average value. 42.2 Second method Colorimetric method
4.2.2.1 Principle
Use three-burning solvent to dissolve: The divalent iron ions in the sample are oxidized to trivalent iron ions, and the trivalent iron ions react with the salt of the associated acid to form a red micro-wavelength complex. The absorbance is measured at a wavelength of 5ILF and compared with the standard series.
4.2.2.2 Reagents
4.2.2.2.1 Acid solution: 10rl1/1.1: accurately collect 83.3mL concentrated salt, add 100ml water. Mix well 2.2.2.2.2 Overload (load 0%).
4.2.2.7.3 Trichloromethane + methyl alcohol (732 mixed solvent): Take 7UmL dichloromethane and 30ml dichloromethane and mix. 4.2.2.2.4 Chloride solution (8.5R/1.1): sieve and weigh 0.35g ferric chloride (FeCl·4I1,1) 100ul into a colorimetric bottle, add water and then add hydrochloric acid solution. Dilute with water until the solution is diluted to 10% (the solution can be stored in a refrigerator at 10℃ and can be stable for more than 1 year).
4.2.2.2.5 Potassium succinate solution 80?/1,): Weigh 50% thiocyanate, add water to dissolve to 50mL<The solution can be stable in the refrigerator at 10℃!
4.2.2.2.6 Standard thread (1.0R/L1): Weigh 0.10g reducing powder in a 100mL beaker, add 10ml. Salt brand (3n./[2.mT-11ml. Hydrogen peroxide (3u% after dissolution on the electric furnace for 5m:n to remove excess hydrogen peroxide: Cool to room temperature and add 10m7. Guest to the flow, dilute with water until it is not thick, accurate hook, this catalytic year is equivalent to 1.0g, 4.2.2.2.7 Iron standard use wave ((.21g/L), weigh 1.0ml. Iron standard catalyst reserve (.0mg/mL) Add 17% ethanol to each volume of the sample (accurate to the scale) and 10% coagulant to each volume of the sample. Mix well. Each volume of the sample is equivalent to 10.0% of the sample. 4.2.2.3 Apparatus 4.2.2.3.1 Photometric tube 4.2.2.3.2 1. Colorimetric tube with stopper: 4.2.2.4 Analysis Steps 4.2.2.4.1 Preparation of sample solution Weigh approximately 0.11% of the sample (accurate to the scale) and 10% chloroform (accurate to the scale) into a volumetric bottle. Add 7% ethanol to the mixture and dilute to the scale. Mix well. The soft band absorbent was used (10.0 μg/m1.) U, 0.2, 0.3, 1.0.2.C.3.0.4.0 L (each equivalent to 0.2..50 10.02..39 0.4).0 g) in 9 ml of dry colorimetric medium, diluted with 1:3 oxane (7+3 agent) to the desired concentration. Then add about 0.15 μL potassium sulfoxide (30/1.). After 5 hours of drying at room temperature (1°C ~ -35), the absorbance was measured in 1 cm2 of water with the ratio of trioxane (713) to the total absorbance at 500 nm. The standard was used. After subtracting the zero absorbance from each absorbance, a standard curve will be prepared or a straight line regression equation will be calculated. 4.2.2.4.2 Sample determination
Pipette 1.1 mL of sample solution into a dry 11 mL colorimetric tube, add 1 drop of (3.05 mL) sodium hydroxide (3.5 g/L) solution, add trioxymethylene glycol, add 3% sodium hydroxide solution to a weak cup until pressure is reached, mix, and then follow the method of 4.2.2.4.1 "add 1 bottle of about 2.05 mL of the sieve 20/)... After subtracting the zero absorbance from the sample absorbance, compare it with the curve and use the regression equation to obtain the content.
GB/T 5009.37—2003
4.2.2.5 Calculation of results
Calculate the oxidation value of the sample according to the formula (): x=
×55.34×?
Select the equivalent value, the unit is the gram equivalent per gram of gold (k) obtained from the standard curve, the unit is microgram): the amount of zero iron is obtained on the standard curve, the unit is microgram (1B); the total volume of the sample is in milliliters, the maximum sample volume during the measurement is in milliliters (m1); - sample mass, the unit is gram (g):
55.84..Fu atom amount;
2—Calculate the factor
4. 2. 2. 6 Precision
The absolute difference between two independent results obtained under repeatability conditions shall not exceed the calculated average value. 4.3 Precision
4.3.1 Principle
The reaction product of these compounds and ?, di-di ... 2.2 Refined extract: Take 5 ml of the solution, add 1000 ml of the stock solution into a bucket, add 50 ml of acetic acid, shake carefully, and release the gas when the mixture begins to evaporate. Separate the layers, discard the last layer, add 50 ml of sulfuric acid and treat again, add the next layer into another bucket, wash three times with water, then dehydrate with anhydrous sodium sulfate, and use a glass to obtain a yellow shrinkage dye solution. 4.3.2.3 2,4-Dichlorobenzene solution: weigh 1,2-dichlorobenzene and dissolve it in 10 ml of refined extract. 4.3.2.4: Weigh 4% solid chloroacetic acid and add 10% sodium hydroxide to decompose it. 4,3.2.5 Potassium-beta-hydroxybenzoate, weigh 4% potassium hydroxide and add 132% ethanol to decompose it, cool it overnight, and collect the upper part to clarify the secretion. If the solution is exhausted, re-prepare. 4.3.3 Spectrophotometer, 4.3.4 Analysis steps Weigh about 0.02-0.5 μg/ml of the sample, add 5.0 mL of the undissolved sample and dilute to the mark, put it into a 2 ml test tube, mix with 3 mL of trifluoroacetic acid and 5 mL of 2,4-dihydroquinone solution at 60 °C for 1 h: After cooling, add T. hydroxide ions to the test tube membrane slowly to form a thin layer, seal it, shake vigorously to mix, and measure the absorbance at a wavelength of 44 nm using a 1 cm colorimetric marker and a test blank as the measuring point. 4.3.5 Aggregate calculation
The base value of the sample is calculated in reverse order according to the formula:
354XV-/V
X: - base value of the sample, unit weight per gram (meo/ke) rA· absorbance of the timed sample bottle
is recorded, unit is gram (g
V, - total volume of the sample, unit is literFu originates from the amount;
2—According to the calculation factor
4.2.2.6 Precision
The absolute difference between two independent complete results obtained under repeatability conditions shall not exceed the calculated average blood value%. 4.3 Basic value
4.3.1 Principle
What compounds and? , the reaction product of the two broken stem cakes, in the reduced liquid is red or wine red, discard 445nTI, determine the wealth of the light, the base price,
4.3.2 Reagents
4.3.2.1 Prepare ethanol, collect 1330l of anhydrous alcohol, measure 2000ml of bottom flask, add 5 more pots of powder, 193% potassium oxide, connect the reflux condenser with the standard push rate, heat in a water bath for 1h, then use the whole slope full steam bag to evaporate the collection case to filter, 4.3. 2.2 Refined extract: Take 5 ml of the solution, add 1000 ml of the stock solution into a bucket, add 50 ml of acetic acid, shake carefully, and release the gas when the mixture begins to evaporate. Separate the layers, discard the last layer, add 50 ml of sulfuric acid and treat again, add the next layer into another bucket, wash three times with water, then dehydrate with anhydrous sodium sulfate, and use a glass to obtain a yellow shrinkage dye solution. 4.3.2.3 2,4-Dichlorobenzene solution: weigh 1,2-dichlorobenzene and dissolve it in 10 ml of refined extract. 4.3.2.4: Weigh 4% solid chloroacetic acid and add 10% sodium hydroxide to decompose it. 4,3.2.5 Potassium-beta-hydroxybenzoate, weigh 4% potassium hydroxide and add 132% ethanol to decompose it, cool it overnight, and collect the upper part to clarify the secretion. If the solution is exhausted, re-prepare. 4.3.3 Spectrophotometer, 4.3.4 Analysis steps Weigh about 0.02-0.5 μg/ml of the sample, add 5.0 mL of the undissolved sample and dilute to the mark, put it into a 2 ml test tube, mix with 3 mL of trifluoroacetic acid and 5 mL of 2,4-dihydroquinone solution at 60 °C for 1 h: After cooling, add T. hydroxide ions to the test tube membrane slowly to form a thin layer, seal it, shake vigorously to mix, and measure the absorbance at a wavelength of 44 nm using a 1 cm colorimetric marker and a test blank as the measuring point. 4.3.5 Aggregate calculation
The base value of the sample is calculated in reverse order according to the formula:
354XV-/V
X: - base value of the sample, unit weight per gram (meo/ke) rA· absorbance of the timed sample bottle
is recorded, unit is gram (g
V, - total volume of the sample, unit is literFu originates from the amount;
2—According to the calculation factor
4.2.2.6 Precision
The absolute difference between two independent complete results obtained under repeatability conditions shall not exceed the calculated average blood value%. 4.3 Basic value
4.3.1 Principle
What compounds and? , the reaction product of the two broken stem cakes, in the reduced liquid is red or wine red, discard 445nTI, determine the wealth of the light, the base price,
4.3.2 Reagents
4.3.2.1 Prepare ethanol, collect 1330l of anhydrous alcohol, measure 2000ml of bottom flask, add 5 more pots of powder, 193% potassium oxide, connect the reflux condenser with the standard push rate, heat in a water bath for 1h, then use the whole slope full steam bag to evaporate the collection case to filter, 4.3. 2.2 Refined extract: Take 5 ml of the solution, add 1000 ml of the stock solution into a bucket, add 50 ml of acetic acid, shake carefully, and release the gas when the mixture begins to evaporate. Separate the layers, discard the last layer, add 50 ml of sulfuric acid and treat again, add the next layer into another bucket, wash three times with water, then dehydrate with anhydrous sodium sulfate, and use a glass to obtain a yellow shrinkage dye solution. 4.3.2.3 2,4-Dichlorobenzene solution: weigh 1,2-dichlorobenzene and dissolve it in 10 ml of refined extract. 4.3.2.4: Weigh 4% solid chloroacetic acid and add 10% sodium hydroxide to decompose it. 4,3.2.5 Potassium-beta-hydroxybenzoate, weigh 4% potassium hydroxide and add 132% ethanol to decompose it, cool it overnight, and collect the upper part to clarify the secretion. If the solution is exhausted, re-prepare. 4.3.3 Spectrophotometer, 4.3.4 Analysis steps Weigh about 0.02-0.5 μg/ml of the sample, add 5.0 mL of the undissolved sample and dilute to the mark, put it into a 2 ml test tube, mix with 3 mL of trifluoroacetic acid and 5 mL of 2,4-dihydroquinone solution at 60 °C for 1 h: After cooling, add T. hydroxide ions to the test tube membrane slowly to form a thin layer, seal it, shake vigorously to mix, and measure the absorbance at a wavelength of 44 nm using a 1 cm colorimetric marker and a test blank as the measuring point. 4.3.5 Aggregate calculation
The base value of the sample is calculated in reverse order according to the formula:
354XV-/V
X: - base value of the sample, unit weight per gram (meo/ke) rA· absorbance of the timed sample bottle
is recorded, unit is gram (g
V, - total volume of the sample, unit is litermL of propylene is diluted to 50Cml with water. 4.4.1.3.2 Cotton acid standard solution: accurately weigh .10 (cottonpol, fish in a 1mL container, add ketone; 7U> dissolve and dilute to the scale. This solution is equivalent to 1,mR ketone, 4.4.1.3.3 Cotton age standard solution: absorb cotton with standard solution 5.mL. Monitor in a 109mL container, add (70% by weight) with group science, this is equivalent to 5, cotton before, 4.4.1.4 Analysis steps
Weigh 1,0 refined oil. 2 crude oil is placed at 0 m. Add 2 μL of acetone (1 μL/min) to a conical flask and shake at a speed of 1500 nm. Then place it in a refrigerator overnight. Take the above solution and filter it. The solution is used for determination.
Pipette 0.013.200, U..1.6.2.4 ml of phenol standard solution (equivalent to 0.5102, 20120R phenol) and add 1 μL of acetone (7%) to each sample. Shake for 1 min. Take the sample and standard solution and filter it into a colorimetric cup. Measure the absorbance at the wavelength of 378 nm with acetone (72%). Draw a standard curve. 4. 4. 1. 5 Calculation of results
The amount of free ions in the test column is calculated according to formula (3). × 123911002
The amount of free ions in the selected sample, in grams per gram (g/13); The mass of free ions in the sample obtained under constant pressure, in micrograms (μg): The mass of the sample, in units of μg (R),
The calculation retains one significant figure.
4.4.1.6 Precision
The absolute value of two independent determination results obtained under repeatability conditions shall not exceed 10 of the average value of the two determinations. 4.4.2 Benzene method
4.4.2. 1 Principle
After the free cottonpol in the sample is collected, it reacts with amines in the ethanol solution to form a yellow compound, which is more similar to the standard series. 4.4.2.2 Reagents
4.4.2.2.1% is added to 0.
4.4.2.2.22 Alcohol (553,
GRT 5009.37-2003
4.4.2.2.3 Unamined: Reduce too much or filter, the color is darker than the heat. 4.4.2.2.4 Standard phenol drop: To 1, 4.1.3.2 4.4.2.3. .1,4.4.2.3 Analysis steps
Take about 1.c only) 5-a system, add 20,m. two broken glass beads, and violently drop them, and put them in the refrigerator overnight, select and equalize the pressure of the liquid. In two 25m colorimetric tubes, if .0m is added, the filter is used as the middle tube, the other is C1.10.0.20C.4.0.80, 1.00mL of the standard reduction is made into 4C, 5.G, 16.0, 20.0.40., 5 ((code cotton resistance)) in each of the two groups of 25m colorimetric tubes (7%) to 2m group standard tube sample tube 1 person 3m1. Collect, heat at 1=mis), take out the cold single end, add 25m1.7% ethanol to each standard test tube to 25ral. After adding ethanol, both sets of standard test tubes are replaced for 15min. Use the standard before as the reagent, use the 1c colorimetric cup as the reagent, adjust the zero point with the standard zero tube, wavelength at 445, measure the photometry of the two: use the two compositions of the aurora to compare with the standard concentration and the shadow of the test tube with it from the standard curve to find out the content of the microstructure from the standard curve: 4.4.2.4 Calculation of results: The content of microstructured carbonyl alcohol in the middle reaches must be calculated by the formula (through the test tube). The formula is: ×1 000 ×1 000 ×270 ×195 The content of high-risk substances in the sample is g/kg (100:100 μg): The unit is μg. The calculation results retain the three valid numbers: 4.4.2.5 Concentration The absolute difference between the results obtained under the conditions of high efficacy and the arithmetic half-mean shall not exceed 4.5 Arsenic The content of high-risk substances in the sample is g/kg (100:100 μg): The unit is μg. 4.7 Retention of solvents 4.8.1 Principle
The sample can be sealed and cut off. At a constant temperature, the sample is vaporized to a uniform volume. The sample is measured in the liquid above the sample. The effluent can be quantitatively measured. 4.8.2 Reduction
4.8.2.1 DMA) Collect 1.0ml in an empty bottle and place it at 60°C. Collect 1L of the sample and the sample can be used at 0-100°C. If there is any disturbance, it can be treated with ultrasonic or heated by air. 2.8.2.2 Take 2mL of the cleaned H2O in the vaporized bottle and put it in the bottle. The mass of the H2O in the vaporized bottle is 2mL. The mass of the H2O in the vaporized bottle is 1mL. The mass of the H2O in the vaporized bottle is 1mL. The mass of the H2O in the vaporized bottle is 1mL. The mass of the H2O in the vaporized bottle is 1mL. The sample can be accurately weighed with a flow rate of about 100ml. Use an instrument to take a sample with a flow rate of about 100ml before filling. Do not let the sample come into contact with the liquid. And formula 8: calculate the liquid density of the input multi-dose: .e-mJro.sanx1000
wherein:
the concentration of the No. 6 final dose, the unit is the set point per liter: ml.): the unit of a bottle and room is
the mass development of MA, the unit is gram
the measurement of the added agent, the unit is);
4MA at 2 o'clock, the unit is gram per liter ml4.8.3 Instrument
4.8.3.1 The rent of the gasification bottle (penetrating bottle) is 0m1.~15T.Bing case GB/T 5009.37-2003
Dense test: put 1m hexane in low density hot water (3cm) 4.8.3.2 Gas chromatograph: with hydrogen ionization detection: 4.8.4 Analytical steps
4.8.4.1 Gas chromatograph test conditions
4.8.4.1.1 Package, steel sample. Inner diameter 3mm, length m, inside 59DEG5 of it 102G2.30 days 4. B. 4. 1. 2
4.8. 4. 1. 3
Equipment: Micro-detonation test station.
Oxygen deviation: 50..
4.8.2, 1.4 vaporization room temperature, 140%:
4.8. 4. 1.5NLn
4.8.4. 1.6 Gas,: 50 ml.minWww.bzxZ.net
4. 8. 4. 1.7 Gas penetration: 5) rm)./1min4.8.4.2 Determination
Practical oil seal of the oil is collected in a 5-position temperature box and then collected by a micrometer or instrument for 1m~, mT hungry gas length (with the standard curve according to the machine-injected gas full, record the sheep component or multiple E points (music normalization method> measurement sensitive peak activity or this and this, compare with the cup standard curve, calculate the total amount of gas on the liquid. 1
Report rubber ticket
Sweet face||tt ||Figure 1 Gasification dead
4.8.4.3 Standard test curve test
Take the first positive phase package spectrum only on the table eight loss rate before the amount of hinge low certificate for the mountain reduction preparation of the body bottom oil I through the 7 open cylinder control large residual Lang rate agent edible oil bed building standard, respectively you happy 25, 08 release 6 culture gasification in the case room, must pass the rate of the juice person six ball business (8220 years divided into, 2×X, 0.1 for six s::
GB/T 5009.37—2003
The freezing point of the agent is placed in a 50°C oven, and 330°C of standard gas is injected into the sample. The corresponding values are deducted from the blank sample to prepare the standard solution (multiple chromatograms are calculated by normalization method). 4.8.5 Calculate the content of the sample by the formula (). 0×1U0K
Where:
X—the content of the six bases in the oil sample, in milligrams and kilograms; RR determination of the mass of the gasifier in the gasification bottle, in micrograms (ig); m
The mass of the sample, in grams ().
The calculated result retains three valid digits,
4.8.6 Precision
The difference between two independent determination results obtained under the significance test shall not exceed 5% of the arithmetic mean. 4.9 Sugar (applicable to margarine)
Replace according to 63/TE00138.
4.10 Identification of non-edible oils in oil
For the three types of non-edible oils in the market: qualitative identification, 4.10.1 Tung oil
4.10.1.1 Trichloroethane-monochloroethane interface method: Take 1 ml of oil sample and put it into a test tube. Add 1 ml of trichloroethane along the test tube.), so that the liquid in the test tube is separated into two layers, and then heated in water for about 1 minute. If there are bubbles, the two layers will fall on the surface of the separated surface and a purple-red to dark coffee-colored ring will appear.
4.101.2 Industrial nitrate, the word is used to detect the oil in dark oils such as soybean oil and cotton oil. But it is not suitable for the detection of dry oil or art pool. Take 5~1 drop of the test tube. If the oil is dissolved in nitrite, there will be a sediment. After passing through the pool for 1 time, there will be a little nitrite and sulfuric acid. If there is oil in the liquid, there will be a white precipitate. It will start to show color and turn yellow after standing.
4.10.1.3 Sulfuric acid method, take a few drops of the sample, put it on a white porcelain plate, and scratch it with 1~2 drops. If there is oil, a dark red solid will appear. The color will be Deepen, and finally become a color without color. 4.10.2 Mineral Dis
Take 1 test sample, add 1mL of hydrogenated saponin solution (600/> and 25mL of ethanol. Connect to air cooling source and reflux for about 5min. It should be heated evenly during saponification for a short time. After saponification, add 25mL of boiling water, and ditch. If it is turbid or there is a precipitate of a foamy substance, it means that there is a substance that cannot be saponified.
4.10.3 Hemp Oil
Take 10μl of test and control hemp oil and spot it on a silica gel G thin layer plate. The thickness of the thin layer plate is 3.25mm~0.3mm. Activate at 105°C for 30min. Adjust the viscosity by b times, and then spot the sample. The spotting amount is about 13-20 μl. Do not use an agent. The color developer is a solid solution (>.)). When the step point and the control color are equal, it means that there is marijuana, sesame oil, sesame oil private prison shop blue B also measures red, and the transmission is smaller on the thin layer plate. 4.11
GH/T500,22 operation,
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