Some standard content:
National Standard of the People's Republic of China
Survey Specification
Biological Survey
The specification for oceanogrephic surveyMarine biologicel survey
1 Subject content and scope of application
GB 12763.6—91
This standard specifies the technical requirements, sampling, analysis and data processing of marine biological surveys. This standard applies to marine biological surveys in surveys of basic elements of the marine environment. 2 Reference standards
GB 2014 Technical requirements for silk and synthetic fiber screensGB12763.1 Marine survey specifications: General
GB12763.7 Marine survey specifications Marine survey data processing 3 Language symbols
3.1 Chlorophyll
Chlorophyll is a very important pigment in autotrophic plant cells and is the main substance for absorbing and transmitting light energy when plants perform photosynthesis. Chlorophyll a is the main pigment. Chla stands for chlorophyll a in seawater. 3.2 Primary productivity
The ability of phytoplankton to assimilate inorganic carbon per unit volume of seawater (or per unit area of sea area) per unit time. 3.3 Productivity index
The ability of chlorophyll a per unit time to assimilate inorganic carbon. 3.4 Microorganisms
A group of single-cell or multi-cell organisms with tiny individuals, simple structures and diverse physiological types. 3.5 Bacterial heterotrophic growth rate
The rate at which heterotrophic bacteria use organic matter for growth and reproduction. 3.6 Bacterial heterotrophic activity
The ability of heterotrophic bacteria to carry out physiological metabolic activities. 3.7 Plankton
A group of organisms that lack developed movement organs and have very weak movement ability and can only move with the water flow and passively float in the water layer. 3.8 Benthic organisms
A general term for organisms living on the surface of the ocean base or in the sediment. According to the size of the individual, the organisms that are blocked by the mesh of the sieve with a hole width of 0.5mm are called macrobenthic organisms. The dust that can pass through the mesh of the sieve with a hole width of 0.5mm and is blocked by the hole width of 0.042mm is called microbenthic organisms.
Approved by the State Administration of Sport on March 22, 1991 and implemented on January 1, 1992
:com3.9 Pollutant organisms
GB12763.691
Organisms that live on the bottom of ships and the surface of all facilities in the water. These organisms are generally harmful. 3.10 Swimming animals
A general term for a class of animals that have well-developed motor organs, can swim freely, and are prone to changing habitats. Part I General Provisions
Technical Design
Technical design should be carried out according to the survey tasks, including station establishment, projects, content, methods, time, number of times, expected results, professional configuration, personnel quality, ships and equipment. Special attention should be paid to the technical requirements and guarantee measures for sea sampling and indoor analysis. 5 Survey Requirements
5.1 Survey Projects
5.1.1 Chlorophyll and Primary Productivity
5.1.2 Microorganisms
5.1.3 Plankton
5.1.4 Large and Small Benthic Organisms
5.1.5 Fouling Organisms
5.1.6 Swimming Animals
5. 2 Auxiliary Parameters
When surveying marine organisms, relevant professional parameters should be determined as needed and observations should be carried out simultaneously. 5.3 Survey methods
5.3.7 Large-scale observation
5.3.2 Section observation
5.3.3 Continuous observation
5.4 Sampling methods
5. 4. 1 Sampling
Applicable to sampling of chlorophyll concentration and primary productivity, microorganisms, phytoplankton, etc. Sampling shall be carried out according to the specified water layer (see Table 1) Table 1 Sample layer
Water depth range
100-200
Standard time
Surface layer 510 Room layer
Surface layer 5102030 Bottom layer
Surface layer 510203050
75 Bottom layer
75100150
Surface layer 510203050
Note: @Surface layer refers to the water layer within 0.5m depth below the sea. ② When the water depth is less than 50m, the bottom layer is the water layer 2㎡ from the bottom. ③ When the water depth is 50～200, the distance between the bottom layer and the bottom is 4% of the water depth. 5.4.2 Trawl
Applicable to sampling of plankton, benthic organisms and swimming animals. 5.4.3 The minimum distance between the bottom layer and the bottom layer of the mud sampling is applicable to sampling of microorganisms and benthic organisms.
5.4.4 Sampling on the hanging board and the water surface or underwater facilities is applicable to sampling of fouling organisms.
5.5 Survey time
5. 5. 1 Monthly survey.
GB 12763. 6 --91
5.5.2 Quarterly survey, generally February (winter), May (spring), August (summer), November (autumn). The time intervals of surveys in each season should be basically equal. If there are special requirements, the number of surveys should be increased according to the situation. 5.5.3 In sea areas far away from my country, the survey time should be determined according to the actual situation. 5.6 Positioning requirements shall comply with the relevant provisions of GB12768.1. 5.7 Preparation for the sea
The items needed at sea should be calculated in practical and reserve quantities, and instruments and equipment should be installed and stored, with safety and convenience as the principle. 6 Main instruments and equipment
6.1 Marine biological mixing and analysis, mainly including water samplers, bottom sediment samplers, fluorometers, spectrophotometers, liquid scintillation counters, various nets and accessories, biological classification and identification, counting and measurement, weighing equipment, centrifugation, desiccation, refrigeration, drying and other equipment. Instruments and equipment, standard substances and reagents shall comply with the relevant provisions of GB12763.1. 6.2 Survey ships
6.2.1 Laboratory
6.2.1.1 General laboratory
Applicable to the processing and analysis of samples of quercetin, benthic organisms, fouling organisms and zooids. 6.2.1.2 Radiation laboratory
Applicable to the analysis and measurement of primary productivity and microbial samples using +C and H, and is required to have ventilation and radioactive protection facilities. The radiation laboratory and the instruments that come into contact with C and H must be clearly marked and the radiation intensity must be tested regularly. 6.2.1.3 Microbiological laboratories are suitable for the processing of microbiological samples and should have shade-free facilities. 6-2.2 Other facilities 6.2.2.1 The winch, wire rope, net-raising rod, double trawl or single trawl (including plate net frame) and fish finder, etc., must meet the requirements of swimming animal trawl. 6.2.2.2 The power winch, wire rope, boom, sea (fresh) water source, working power supply, deck work space and location, etc., must meet the requirements of various professions. 7.1 Sampling requirements 7.1.1 The sampling location should avoid the sewage outlet of the survey ship.
7.1.2 Deep water sampling
Use the water sampler specified in the survey project to collect water. Before entering the water, check whether the water sampler is closed and accurately place it in the predetermined water layer. Strictly observe the stagnation time. Take samples and process them as required.
7.1.3 Trawl sampling
Use the nets specified by the professional to collect samples, strictly control the speed of raising and lowering the net, and accurately judge whether the net has reached the predetermined water layer. When trawl, pay attention to whether the working status is normal. If there is any abnormality, take effective measures immediately. Rinse the nets carefully, collect samples, and strictly prohibit specimens. 7.1.4 Bottom sampling
Use the sampler specified in the project to collect samples, strictly abide by the operating procedures, and pay attention to the working status of the sampler. Take samples and process them as required. If abnormalities are found, re-sample.
7.1.5 Sampling on hanging plates and underwater facilities
GB 12763. 6—91
Make plates according to the plan requirements, correctly select the hanging plate location, sampling facilities and hanging plate methods. Strictly take plates, sampling time and sample processing. 7.2 Record sampling
Record according to the relevant provisions of GB12763.1. In case of abnormal phenomena or new discoveries, in addition to recording, take photos or videos on the spot. 7.3 Requirements for sampling tools and equipment
According to the relevant provisions of GB12763.1.
8 Sample analysis
8.1 Sample processing
The samples collected from each project should be processed according to professional requirements. 8.2 Sample determination
The samples to be determined should be determined according to professional requirements. 8.3 Identification and counting
Main species. Except for microorganisms, they should generally be identified to species and counted according to professional requirements. 8.4 Sample preservation
Samples after analysis, measurement and identification can be retained in whole or in part according to data analysis, application level and academic value. Retained samples should be kept as required.
9 Data collation and report writing
9.1 Data collation
9.1.1 Calculation and statistics
Identify, count and measure results, calculate and count according to the prescribed formula and format. 9.1.2 Fill in recommended forms
Fill in various calculations and statistical results according to the requirements of the form and card. 9.1.3 Draw charts
After the relevant data and information are formed, draw the Shu-style chart according to the requirements. 9.1.4 Fill in reports
Fill in various reports according to the relevant provisions of GB12763.1. 9.2 Write reports
9. 2. 1 Voyage report
After each voyage marine survey is completed, the technical person in charge shall write a voyage report according to the relevant provisions of GB12763.7. 9.2.2 Survey results report
After the survey task is completed, the technical person in charge shall write a marine survey results report according to the relevant provisions of GB12763.」. 9.3 Data archiving, acceptance and results appraisal
According to the relevant provisions of GB 12763.1.
Part II Determination of Chlorophyll and Primary Productivity 10 Technical Requirements
10.1 Determination of chlorophyll and primary productivity includes determination of chlorophyll a and primary dust productivity. 10.2 Determination of Chlorophyll a
10.2.1 Sampling Level
See Table 1.
10.2.2 Precision
GB 12763. 6—91
When the chlorophyll a concentration is at the level of 0.5 mg/m*, the relative error of repeated samples is ±10%. 10.3 Determination of Primary Productivity
10-3.1 Sampling Level
According to the optical depth, water samples shall be collected at the depths where the light intensity is 100%, 50%, 30%, 10%, 5% and 1% of the surface layer. Special cases may be determined according to regulatory requirements.
10.3.2 Determination range
0.05~100mg/(mh).
10.3.3 Precision
When the primary productivity is at the level of 30mg/(m2·h), the relative error of repeated samples is ±10%. (Cultivated for 3h, 185kBg radioactive\c added)
11 Determination of chlorophyll in seawater (extraction fluorescence method) 11.1 Principle of the method
The acetone extract of chlorophyll a is excited by blue light to produce red fluorescence. The phytoplankton obtained by filtering a fixed volume of seawater is extracted with 90% acetone. The fluorescence value of the extract before and after acidification is measured using a fluorometer to calculate the concentration of chlorophyll in seawater. 11.2 Main instruments and equipment
11. 2. Fluorometer
The excitation light wavelength is 450nm and the emission light wavelength is 685nm. 11.2.2 Filtration device
Includes filter, support, flask and vacuum pump. 11.2.3 Glass fiber filter membrane
Its interception efficiency is equivalent to that of polycarbonate microporous membrane with pore size of 0.65 um. 11.2.4 Refrigerator
11.3 Reagents
11.3.1 90% (V/V) acetone
11.3.2 10% (V/V) hydrochloric acid
11.3.3 Magnesium carbonate minus p (MgCOa) = 10 g/dm11.4 Determination steps
11.4.1 Fluorometer calibration
11.4.1.T Calibrate at least once every six months. 11.4.1.2 Preparation of standard chlorophyll a solution (p = 1 mg/dm\) Filter and quantify the chlorophyll extracted from well-grown cultured diatoms in the early stage of exponential growth with 90% acetone, or dissolve a certain amount of commercially available chlorophyll a crystals with 90% acetone, the concentration of which is about p = 1 mg/dm. 11.4.1.3 Calibration of standard chlorophyll a solution Use a spectrophotometer to correctly determine the concentration of the standard chlorophyll a solution. 11.4.1.4 Preparation of chlorophyll a standard working solution Use the above-mentioned standard chlorophyll a solution to prepare standard working solutions of different concentrations for calibration of each range. 11.4.1.5 Determination of conversion factor F. The above-mentioned standard working solutions of different concentrations are used to measure the fluorescence values before and after acidification on different ranges. The calculation formula of the conversion factor Fa of each range is:
Where: F-
GB 12763.6-91
conversion factor of range \d\, m/m*; p(chla)
p(Chla) standard working concentration of chlorophyll a, mg/m; R, fluorescence value before acidification;
R2 fluorescence value after acidification:
11.4.2 Water sample determination
11.4.2.1 Sampling
Sampling shall be carried out at the depth specified in Table 1 of Part I and recorded in the marine table] (see Appendix G). 11.4.2.2 Filtration
After sampling, filtration shall be carried out as soon as possible. The volume of filtered seawater depends on the Shecha Sea area. For sea areas with a water depth of less than 200m, 20 to 100cm is taken, and for sea areas with a water depth of more than 200m, 100 to 250cm is taken. Before filtering, add 1 to 2cm of magnesium carbonate solution. The negative pressure of the suction should be less than 50kPa during filtration, and record it in the marine table 2 (see Appendix G).
11. 4.2.3 Extraction
Put the filter membrane containing phytoplankton into an extraction bottle with 10cm of 90% propylene, cover it tightly, shake it, and immediately put it in a low-temperature (≤0℃) refrigerator for extraction for 12 to 24h.
11.4.2.4 Fluorescence measurement
The measurement steps are as follows:
Take out the sample and place it in a dark place at room temperature for about 0.5h to make the sample temperature consistent with room temperature! b.
Before and after each batch of samples is measured, 90% acetone is used as a comparative solution. The blank fluorescence values Fm and Fz of each range are measured; the supernatant in the extraction bottle is poured into the measuring cell, and the appropriate range is selected to measure the fluorescence value of the sample. The method is: add 1 drop of 10% hydrochloric acid to the measuring cell, and measure its fluorescence value R after 30 seconds. The results are recorded in Table 3 (see Appendix G). 12 Data Arrangement
12.1 Data Calculation
12. 1. 1 Calculation of Chlorophyll a Liquid Content in Seawater F) -(R-- R.) . V)
Where: p.(Chla)--Chlorophyll a concentration in seawater, mg/m\sConversion coefficient of base range\d\, mg/rm*--Fluorescence value before acidification;
R. —Inflammation value after acidification
V,-volume of extract, cm\
V: volume of filtered seawater, cm.
12. 1. 2 Calculation of chlorophyll a content in water column A(Chla)
Where: o-
p.(Chla) + Pa+(Chla). (D-1 = D)2
Chlorophyll a content in water column, mg/m;
Pm(Chla)-
Chlorophyll a concentration in the ith layer, mg/m
D,————depth of the ith layer, ml
number of sampling layers;
x -.
12.1.3 Calculate the average concentration of chlorophyll a in the water column GB12763.6-91
pchla)
p,(Chla)-
Where: p(Chla)—average concentration of chlorophyll a in the water column, mg/m2; A(Chla) chlorophyll a content in the water column, mg/m2; D
—maximum sampling depth, m.
12.2 Fill in the marine table 7 (see Appendix G).
12.3 Draw the distribution map
12.3.1 Plane distribution map
12.3.1.1 Distribution map at each level
Contour line value standard, 0.10.0.20,0.30,0.50,0.75,1.00,1.50,2.003.00,5.00.10,00mg/m12.3.1.2 Content distribution map
Contour line value standard, 1.00,1.50,200,3.00,5.00,10.00,20.00,30.00.50.00,100.00,200.00，300.00,500.00 mg/m.
12.3.2 Cross-sectional distribution map
Contour line value standards: 0.10, 0.20, 0.30, 0.50, 0.75.1.00, 1.50, 2.00, 3.00, 5.00, 10.00 mg/m. 12.3.3 The above value standards can be increased or decreased according to the specific situation. 13 Determination of marine primary productivity (1\C tracer method) 13.1 Principle of the method
A certain amount of radioactive ammonium salt of magnetic acid H\CO- or carbonate 1+CO is added to a water sample with a known total carbon dioxide concentration. After a period of incubation, the amount of organic 1C in phytoplankton cells is determined, and the amount of organic carbon synthesized by phytoplankton through photosynthesis can be calculated. 13.2 Main instruments and equipment
13.2.1 Underwater photometer, underwater illuminance meter or transparency disk. 13.2.2 Sample incubator or culture bottle cover
Use neutral light-attenuating material to reduce the light to 100%, 50%, 30%, 10%, 5% and 1%. 13.2.3 Filtration device
See 11.2.2.
13.2.4 Filter membrane
See 11.2.3 or cellulose ester microporous filter membrane with a pore size of 0.65 μm. 13.2.5 Liquid scintillation counter
13.2.6 Fume hood
13.2.7 ShakerbzxZ.net
13.3 Reagents
13.3.1 Filtered seawater
Use seawater from the surveyed sea area and filter it through a glass fiber or cellulose ester microporous filter membrane. Store it in a clean reagent bottle and wash bottle for storage. 13.3.2 1C working solution
GB 12763. 691
Dilute 1*C stock solution with filtered seawater to make the radioactivity intensity about 1850 kBq/cm, or as required. 13.3.3 Scintillation fluid
13.3.4 Total count scintillation fluid
Add 0.2 cm (V/V) phenylethylamine to every 10 cm\ of scintillation fluid. 13.3.5 0.1 mol/dm hydrochloric acid
13.4 Determination steps
13.4.1 Determination of quenching correction equation
Use a series (usually 6)\C quenching standard bottles and measure on a liquid scintillation counter. Use linear regression method to obtain the equation for calculating efficiency. 13.4.2 Determination of sampling depth
Use an underwater photometer or illuminometer to measure the intensity of light radiation at different depths in the water column, or use a transparency disk to measure the transparency of seawater to determine the optical depth of sampling.
13.4.3 Water sample determination
13. 4. 3. 1 Sampling
Sampling should be carried out at the predetermined depth and recorded in the swimming table 2 (see Appendix G). When sampling, a water sampler that is opaque and has no steel parts should be used: water samples should be protected from direct sunlight.
13.4.3.2 Water sample packaging
After sampling, filter the water sample through a sieve with a pore size of about 180um under weak light as soon as possible and dispense it into culture bottles. The culture bottles must be cleaned and dried, and soaked in 2% (V/V) dilute hydrochloric acid for more than 24 hours. Each layer of samples should include two white bottles and one black bottle. The first and fourth layer samples should also be divided into a zero-time culture bottle. The volume of water samples in the zero-time culture bottle must be accurate. The remaining water samples shall be measured according to the provisions of Chapter 11. The chlorophyll a concentration of each layer of water samples shall be measured.
13. 4. 3. 3 Add 1*C working solution
Take the same volume of 1C working solution and add it to each culture bottle. The amount of phytoplankton added depends on the amount of phytoplankton in the sample and the culture time. Generally, in the sea area with a water depth of less than 200m, 37~~370kBg is added for 24h of culture, and 370~740kBq is added for the sea area with a depth of more than 200m. 13. 4. 3. 4 Take the total count sample
Use a micro-filter to absorb a certain volume of water sample from each zero-time culture bottle, transfer it into two total count scintillation bottles respectively, cover it tightly, mix it, and use it for radioactivity determination. 13.4.3.5 Cultivation
Put the culture bottles (except the zero-time culture bottles) with \C into the corresponding incubators, or put them into the corresponding culture covers and then put them into the transparent incubators. Note the start time of cultivation. The culture bottles should be placed in a place where the sun is not blocked and the temperature should be kept constant during the cultivation period with flowing surface seawater. The cultivation time is generally between 2 and 24 hours and should be as close to the local noon time as possible. 13.4.3.6 Filtration of zero-time samples
After the start of cultivation, filter the two zero-time samples immediately. The obtained filter membrane containing phytoplankton should be placed in a scintillation bottle. In the fume hood, add 1 cm*0. 1 mol/dm2 hydrochloric acid and cover it after 15 minutes. Or fumigate the filter membrane with concentrated hydrochloric acid vapor in the fume hood for 15 minutes and put it in a scintillation bottle. 13.4 3.7 Filtration
After sample incubation. For the steps of filtering water samples, refer to 13.4.3.6. 13.4.3.8 Determination of radioactivity
Add 10 cm of liquid scintillation liquid to the scintillation bottle containing the filter membrane with phytoplankton, oscillate slowly on a vibrator for at least 20 min, measure with a liquid scintillation counter, and record in Marine Tables 5 and 6 (see Appendix G). 13.4.4 Determination of total carbon dioxide concentration in water samples Determine the salinity of seawater samples and calculate the total carbon monoxide concentration in seawater p(C) = (0.067 $ — 0.05) × 0.96 × 12 000-(5)
GB 12763. 6-91
-total concentration of carbon dioxide in seawater (in C), mg/m Where: p(C)—
S…--practical salinity of toxic water (dimensionless).
14 Data compilation of tough grade productivity
14.1 Data calculation
14. 1. 1 Calculation of the amount of C added
R. - VtX 1 000
Amount of C added kBq
Wherein, R—
the average value of the amount of C measured in each total count flash refining bottle, kBq; V—the volume of the culture water sample, cm\;
the volume of the total count water sample for joint measurement, mm.
14.1.2 Calculation of marine primary productivity
P, - (r. fo) : p(c)
Wherein: P,-marine primary productivity (in C), mg/(m*, h)R-\amount of C added, kBq;
Ratio--average value of 1C radioactivity in white bottle samples, kBq-radioactivity of 1C in zero-time samples, kBgRh --
-total concentration of carbon dioxide in seawater, mg/m; pr)
N—·Cultivation time, h.
14.1.3 Calculation of productivity index
n(Chla)
Productivity index, h-\
Wherein: 1
Primary productivity of water body, mg/Add 37~~370kBg, add 370~740kBq in the sea area above 200m. 13.4.3.4 Take total count samples
Use a micro-filter to take two aliquots of a certain volume of water sample from each zero time culture bottle, transfer them into two total count scintillation bottles respectively, cover them tightly, mix them, and use them for radioactivity determination. 13.4.3.5 Culture
Put the culture bottles with \C (except zero time culture bottles) into the corresponding culture boxes, or cover them with the corresponding culture covers and then put them into the transparent culture boxes. Note the start time of culture. The culture bottles should be placed in a place where the sun is not shaded, and the temperature during the culture period should be kept constant with flowing surface seawater. The culture time is generally between 2 and 24 hours, and as close to the local noon time as possible. 13.4.3.6 Filtration of zero time samples
Immediately after the start of incubation, filter the two zero time samples and place the resulting filter membrane containing phytoplankton in a scintillation bottle. Add 1 cm*0.1 mol/dm2 hydrochloric acid in a fume hood and cover it after 15 min. Alternatively, fumigate the filter membrane with concentrated hydrochloric acid vapor in a fume hood for 15 min and place it in a scintillation bottle. 13.4 3.7 Filtration
After sample incubation, refer to Section 13.4.3.6 for the steps of filtering water samples. 13.4.3.8 Determination of radioactivity
Add 10 cm of liquid scintillation liquid to the scintillation bottle containing the filter membrane containing phytoplankton and oscillate slowly on an oscillator for at least 20 min. Determine using a liquid scintillation counter and record in Tables 5 and 6 (see Appendix G). 13.4.4 Determination of total carbon dioxide concentration in water samples Determine the salinity of seawater samples and calculate the total carbon monoxide concentration in seawater p(C) = (0.067 $ — 0.05) × 0.96 × 12 000-(5)
GB 12763. 6-91
-total concentration of carbon dioxide in seawater (in C), mg/m Where: p(C)—
S…--practical salinity of toxic water (dimensionless).
14 Data compilation of tough grade productivity
14.1 Data calculation
14. 1. 1 Calculation of the amount of C added
R. - VtX 1 000
Amount of C added kBq
Wherein, R—
the average value of the amount of C measured in each total count flash refining bottle, kBq; V—the volume of the culture water sample, cm\;
the volume of the total count water sample for joint measurement, mm.
14.1.2 Calculation of marine primary productivity
P, - (r. fo) : p(c)
Wherein: P,-marine primary productivity (in C), mg/(m*, h)R-\amount of C added, kBq;
Ratio--average value of 1C radioactivity in white bottle samples, kBq-radioactivity of 1C in zero-time samples, kBgRh --
-total concentration of carbon dioxide in seawater, mg/m; pr)
N—·Cultivation time, h.
14.1.3 Calculation of productivity index
n(Chla)
Productivity index, h-\
Wherein: 1
Primary productivity of water body, mg/Add 37~~370kBg, add 370~740kBq in the sea area above 200m. 13.4.3.4 Take total count samples
Use a micro-filter to take two aliquots of a certain volume of water sample from each zero time culture bottle, transfer them into two total count scintillation bottles respectively, cover them tightly, mix them, and use them for radioactivity determination. 13.4.3.5 Culture
Put the culture bottles with \C (except zero time culture bottles) into the corresponding culture boxes, or cover them with the corresponding culture covers and then put them into the transparent culture boxes. Note the start time of culture. The culture bottles should be placed in a place where the sun is not shaded, and the temperature during the culture period should be kept constant with flowing surface seawater. The culture time is generally between 2 and 24 hours, and as close to the local noon time as possible. 13.4.3.6 Filtration of zero time samples
Immediately after the start of incubation, filter the two zero time samples and place the resulting filter membrane containing phytoplankton in a scintillation bottle. Add 1 cm*0.1 mol/dm2 hydrochloric acid in a fume hood and cover it after 15 min. Alternatively, fumigate the filter membrane with concentrated hydrochloric acid vapor in a fume hood for 15 min and place it in a scintillation bottle. 13.4 3.7 Filtration
After sample incubation, refer to Section 13.4.3.6 for the steps of filtering water samples. 13.4.3.8 Determination of radioactivity
Add 10 cm of liquid scintillation liquid to the scintillation bottle containing the filter membrane containing phytoplankton and oscillate slowly on an oscillator for at least 20 min. Determine using a liquid scintillation counter and record in Tables 5 and 6 (see Appendix G). 13.4.4 Determination of total carbon dioxide concentration in water samples Determine the salinity of seawater samples and calculate the total carbon monoxide concentration in seawater p(C) = (0.067 $ — 0.05) × 0.96 × 12 000-(5)
GB 12763. 6-91
-total concentration of carbon dioxide in seawater (in C), mg/m Where: p(C)—
S…--practical salinity of toxic water (dimensionless).
14 Data compilation of tough grade productivity
14.1 Data calculation
14. 1. 1 Calculation of the amount of C added
R. - VtX 1 000
Amount of C added kBq
Wherein, R—
the average value of the amount of C measured in each total count flash refining bottle, kBq; V—the volume of the culture water sample, cm\;
the volume of the total count water sample for joint measurement, mm.
14.1.2 Calculation of marine primary productivity
P, - (r. fo) : p(c)
Wherein: P,-marine primary productivity (in C), mg/(m*, h)R-\amount of C added, kBq;
Ratio--average value of 1C radioactivity in white bottle samples, kBq-radioactivity of 1C in zero-time samples, kBgRh --
-total concentration of carbon dioxide in seawater, mg/m; pr)
N—·Cultivation time, h.
14.1.3 Calculation of productivity index
n(Chla)
Productivity index, h-\
Wherein: 1
Primary productivity of water body, mg/
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