The writing rules for detection and identification of regulated pests
Some standard content:
ICS 65.020. 01
National Standard of the People's Republic of China
GB/T 236352009
Writing rules for detection and identification of regulated pestsIssued on April 27, 2009
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of ChinaAdministrative Committee of Standardization of the People's Republic of China
Implemented on October 1, 2009
GB/T23635—2009
This standard is modified and adopts ISPM No. 27 of FAO/IPPC "Diagnostic protocols for regulated pests" (English version). This standard is consistent with ISPM No. 27 Compared with the new standard, the main differences are as follows: the approval, background, date and acknowledgments of the diagnostic protocol, and publication of the diagnostic protocol have been removed; some normative references have been deleted; the scope has been redefined; the "Appendix I: Key steps for developing diagnostic protocols" of the original standard has been removed. This standard was proposed and coordinated by the National Plant Quarantine Standard Promotion Committee. The drafting units of this standard are: Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Inspection and Quarantine Scientific Research Institute, Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, National Agricultural Technology Extension Service Center, Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard are: Yin Liping, Jiao Jun, Lin Shiming, Ben Shuifang, Yu Ju, Zheng Jianzhong, Wang Fuyang, Wang Xiufen, and An Yuzhu. 1
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Provisions for the preparation of protocols for detection and identification of restricted pests
GB/T 23635--2009
This standard specifies the content and structure of the detection and identification procedures for regulated pests, and specifies the minimum requirements for detection and identification methods.
This standard applies to the compilation of detection and identification procedures for regulated pests in relevant international trade and pest control. 2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. For all the referenced documents with the specified period, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, it is encouraged to The parties to the agreement on this standard shall study whether the latest versions of these documents can be used. For any undated referenced documents, the latest versions shall apply to this standard. (H13/T1.1-2000 Guidelines for Standardization Part 1: Structure and Rules for Writing Standards Plant Quarantine Technical Table (ISPM No. 5, FAO, 2007) 3 Principles and basic framework for writing detection and identification procedures 3.1 Principles
3.1.1 Detection and identification procedures for regulated pests should provide necessary methods and guidance for experts or professionally trained personnel engaged in detection and identification of relevant pests. 3.1.2 The detection and identification methods used in the detection and identification procedures should be tested for "sensitivity, specificity and reproducibility"; the technical data of the selected pest diagnosis methods should be publicly published and confirmed by relevant experts. At the same time, the reoperability of these methods (including the difficulty of operation, time spent and speed) and the relevant knowledge and experience that operators should have should be generally applicable: the selected instruments and the acquisition of control samples, reagents, etc. should all be generally applicable. 3.1.3 Each detection and identification procedure should include more than one method as far as possible to provide for laboratories with different detection capabilities and in different situations. Different situations also include different methods for detection of different developmental stages of pests, and also include situations where the diagnosis results need to be confirmed by several methods.
3.1.4 Each detection and identification procedure should include a description of the relevant information of the pest, including: classification information, host, distribution, biology, record keeping and Corresponding scientific literature. In many cases, it can also be supplemented with information that is more helpful for detection and identification. 3.1.5 The detection and identification procedures should provide relevant information on the sources and acquisition methods of reference materials (such as positive and negative controls, specimens) used to ensure the accuracy of pest identification. 3.1.6 The detection and identification procedures should include safety warnings when using some instruments, chemical reagents, etc. used in the diagnostic methods; it should also include reminders that the use of these instruments and chemical reagents does not exclude other appropriate chemicals or instruments. 1) Experts refer to entomologists, mycologists, virologists, bacteriologists, nematologists, weed scientists, molecular biologists, etc. 2) Reproducibility refers to the degree of consistency between individual results obtained using the same method, the same test materials, and under different conditions. Different conditions refer to different operations, different equipment, different laboratories, and different or same time. 1
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GB/1 23635—2009
3.2 Basic framework
Informative overview elements
Normative general plan
Normative technical elements
Informative supplementary elements,
Note: Elements with * are optional.
Daily"
Standardized name
-Normative reference document "
Terms and definitions"
Pest information
Classification information
Instruments and appliances
Reagents (drugs) and Materials
Symptoms and detection
Records and specimen storage
Normative summary"
Ways to obtain information
Informative appendix
References
-Index*
4 Basic content of informative overview elements
4.1 Cover
The front should comply with the provisions of 6.1.1 in GB/T1.1-2000.4.2 Table of contents
This is an optional element. The following should comply with the provisions of 6.1.2 in GB/T1.1-2000.4.3 Foreword
Each standard should have a foreword, which consists of specific parts and basic parts. The specific part shall give the following information appropriately:
Describe the degree of consistency with the corresponding international standards, guidelines, instructions or other documents, write the number of the corresponding international documents, the Chinese translation of the document name, and list the technical differences with the adopted international standards and the main editorial changes made; ...Describe all or part of other documents replaced or abolished by the standard; Describe the major technical changes compared with the previous version of the standard; Describe the relationship between the standard and other standards or documents. Describe which appendices in the standard are normative appendices and which are informative appendices. The basic part shall give the following information appropriately:
The proposing and responsible unit of the standard;
The drafting unit and drafter of the standard;
The previous versions of the standard replaced by the standard. 2
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4.4 Introduction
The introduction is an optional element and shall comply with the provisions of 6.1.4 in GB/T1.1--2000. 5 Basic contents of the preparation of normative general elements
5.1 Standard name
GB/T 23635—2009
The standard name should be written in accordance with "Procedures (or methods) for the detection and identification of regulated pests (current scientific names and Chinese names)" and the English name should be attached.
5.2 Scope
The main contents of the standard should be clearly stated, and the following usage should be used. "This standard specifies ……\, "This standard is applicable to" the detection and identification procedures of regulated noxious crops. It is applicable to identification or monitoring, etc., and is applicable to local or third-party laboratories; its detection and identification may be related to international trade or to the determination of domestic pest status. 5.3 Normative references
This is an optional element. The principles and specific methods of citing standards can be found in 6.2.3 of GB/T 1.1-2000. 6 Basic contents of the preparation of normative technical elements
6.1 Terms and definitions
This is an optional element. Any term that is not immediately understood or well-known, or a term that may have different interpretations in different situations, or an important professional term in detection and identification, should be defined to clarify. The definition should be headed by the following words: "The following terms and definitions apply to this standard." For quarantine terms and definitions, please refer to ISPM No. 5. 6.2 Pest information
Provide brief information on pests, including the life history of the pest, morphological characteristics, morphological and (or biological) variation, relationship with other related species, general parasitic range, damage to sheep and sheep, current and previous geographical distribution (of the palace), and the mode of transmission and spread (including vectors and pathways). This part of the information is important but should not be too long or too long, and should not exceed one page. 6.3 Classification information
The content mainly includes:
a) The name of the pest, including the name of the current scientific name and the year (fungi include the known asexual and sexual generations): - Synonyms; including former names;
-Recognized common name, the asexual generation name of fungi (including synonyms); - Abbreviations of viruses and viroids;
Chinese name, including former names, common names, pathogen names, disease names, etc. b) Classification status, including relevant subspecies classification information. 6. 4 Instruments and utensils
List the instruments and utensils required for specific diagnostic methods. 6.5 Reagents (drugs) and materials
List the main reagents (drugs) and materials required for the specific diagnostic methods. 6.6 Symptoms and detection
6.6.1 Describe the types and parts of host plants, plant products and other commodities that can carry or be found in pests. 6.6.2 Describe the symptoms or signs of hosts infested by pests, including typical characteristics and similarities and differences with other causes of symptoms. Attach slides, photos, diagrams and other image materials when necessary. 6.6.3 Describe the developmental stage of the detected pests, as well as the possible location and density of the pests on (in) the host plants, plant products and other commodities.
GB/T 23635--2009
6.6.4 Describe the host developmental stage, sputum conditions and seasons related to the pests. 6.6.5 Describe the detection methods of pests in goods, such as using a diopter or a magnifying glass. Describe how to correctly collect and extract pests from infected hosts.
6.6.6 Describe the collection and extraction methods of pests from asymptomatic hosts or other materials (such as soil, water, culture medium). 6.6.7 Describe the activity of detected pests. 6.6.8 Describe the sensitivity, specificity and reproducibility of all methods in this part and the positive, negative quality control substances and reference substances used in the test. The differences with similar symptoms caused by other factors should also be provided. 6.7 Identification
6.7.1 One or more methods that can be used alone to identify pests should be provided. When there are multiple methods, the advantages and disadvantages of these methods should be given, as well as the equivalence of the methods when used alone and in combination. If several methods are required or multiple methods are used alternately to identify pests, a flow chart (table) should be drawn. 6.7.2 Methods in this part: mainly include methods based on morphological characteristics or morphological data characteristics, biological characteristics (such as toxicity or host range), and biochemical and molecular characteristics. Morphological characteristics can be obtained directly or after isolation and cultivation: if cultivation or isolation is a necessary step in the biochemical or molecular method, it should be described in detail. 6.7.3 For identification using morphological and morphological data characteristics, the following information should be provided: preparation of pests, mounting and inspection methods (such as optical, electron microscopy and measurement techniques); identification checklist (family, genus, species); a description of the morphology of the pest or its colony, including pictures of morphological identification characteristics and an explanation of the difficulty in observing specific structures; comparison of similar or related species; a relevant reference specimen or culture.
6.7.4 For each biochemical or molecular identification method (such as serological method, electrophoresis, PCR method, DNA barcoding method, RFLP method, IgG1 sequencing method), a detailed description (including equipment, reagents and consumables) should be given. References to related materials (such as standard substances and reagents to be used) should be attached after the method. 6.7.5 When more than one method can be used reliably, other appropriate methods should be listed as alternative or supplementary methods, such as: there are reliable morphological methods, but there are also appropriate biological methods. 6.7.6 The methods for isolating pests from asymptomatic host plants or plant products (such as detecting potential infestations) should be described in detail, and when necessary, biological methods for directly identifying asymptomatic materials should also be provided. Phytochemical or molecular methods. 6.7.7 Describe in detail the phenomena and problems that may arise in the identification of pests, and provide the contact method of the laboratory for comparison and verification of the test results when necessary.
6.8 Record and specimen sample storage
6.8.1 The record information that should be kept for the pests identified by quarantine includes: a) the name of the identified pest;
b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d)
Description of the signs of damage or damage to the harmful crops (including relevant photos), and if there are no symptoms, it should also be stated: diagnosis Methods used for identification, including quality control materials, and results obtained by the method: f
If morphological or morphometric methods are used for identification, relevant characteristics and measurement data, drawings or photos, and descriptions of the developmental stages of the pests shall be preserved; g
3) Enzyme chain reaction method.
4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results shall be preserved, such as: gel photos based on the identification results or printouts of EI.ISA readout results; descriptions of the extent of damage on the host (how many pest individuals are found, and the tissues and areas affected) , statistics: i
j) Name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as alive or dead, the activity of the developmental stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or printed results of EL.ISA, etc.) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to GI3/T1.12000 6.3.8. 7 Basic contents of the preparation of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative appendix
This is an optional element. Abbreviated according to 6.4.1 in GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Prepared according to 6.1.3 in GR/T 1.1-2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
Ji will be replaced by our publishing center
Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net001. Describe the types and parts of host plants, plant products and other commodities that can carry or be found with pests. 6.6.2. Describe the symptoms or signs of hosts infested with pests, including typical characteristics and similarities and differences with other causes of symptoms. Attach slides, photos, diagrams and other image materials when necessary. 6.6.3. Describe the developmental stage of the detected pests, as well as the possible location and density of the pests on (in) the host plants, plant products and other commodities.
GB/T 23635--2009
6.6.4. Describe the host developmental stage, climatic conditions and seasons related to the pests. 6.6.5. Describe the detection methods of pests in the goods, such as using a diopter or a magnifying glass. Describe how to correctly collect and extract the pests from infected materials.
6.6.6. Describe the collection and extraction methods of pests from asymptomatic hosts or other materials (such as soil, water, culture medium). 6.6.7 Describe the activity of the detected pests. 6.6.8 Describe the sensitivity, specificity and reproducibility of all methods in this section, as well as the positive, negative control substances and reference substances used in the test. The differences from similar symptoms caused by other factors should also be provided. 6.7 Identification
6.7.1 One or more methods that can be used alone to identify pests should be provided. When there are multiple methods, the advantages and disadvantages of these methods should be given, as well as the equivalence of the methods when used alone and in combination. If several methods are required or there are multiple methods to alternately identify pests, a flow chart (table) should be drawn. 6.7.2 Methods in this section: mainly include methods based on morphological characteristics or morphological data characteristics, biological characteristics (such as toxicity or host range), and biochemical and molecular characteristics. Morphological characteristics can be obtained directly or after isolation and cultivation: if cultivation or isolation is a necessary step in the biochemical or molecular method, it should be described in detail. 6.7.3 For identification using morphological and morphological data characteristics, the following information should be provided: preparation of pests, mounting and inspection methods (such as optical, electron microscopy and measurement techniques); identification checklist (family, genus, species); a description of the morphology of the pest or its colony, including pictures of morphological identification characteristics and an explanation of the difficulty in observing specific structures; comparison of similar or related species; a relevant reference specimen or culture.
6.7.4 For each biochemical or molecular identification method (such as serological method, electrophoresis, PCR method, DNA barcoding method, RFLP method, IgG1 sequencing method), a detailed description (including equipment, reagents and consumables) should be given. References to related materials (such as standard substances and reagents to be used) should be attached after the method. 6.7.5 When more than one method can be used reliably, other appropriate methods should be listed as alternative or supplementary methods, such as: there are reliable morphological methods, but there are also appropriate biological methods. 6.7.6 The methods for isolating pests from asymptomatic host plants or plant products (such as detecting potential infestations) should be described in detail, and when necessary, biological methods for directly identifying asymptomatic materials should also be provided. Phytochemical or molecular methods. 6.7.7 Describe in detail the phenomena and problems that may arise in the identification of pests, and provide the contact method of the laboratory for comparison and verification of the test results when necessary.
6.8 Record and specimen sample storage
6.8.1 The record information that should be kept for pests identified by quarantine includes: a) the name of the identified pest;
b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d)
Description of the signs of damage or damage to the harmful crops (including relevant photos), and if there are no symptoms, it should also be stated: diagnosis Methods used for identification, including quality control materials, and results obtained by the identification methods: f
If morphological or morphometric methods are used for identification, relevant characteristics and measurement data, drawings or photos, and descriptions of the developmental stages of the pests shall be preserved; g
3) Enzyme chain reaction method.
4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results shall be preserved, such as: gel photos based on identification results or printouts of EI.ISA readout results; description of the extent of damage on the host (how many pest individuals are found, and the tissues and areas affected) , Statistics: i
j) Name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as alive or dead, the activity of the developmental stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or printed results of EL.ISA, etc.) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to GI3/T1.12000 6.3.8. 7 Basic contents of the preparation of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative appendix
This is an optional element. Abbreviated according to 6.4.1 in GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Prepared according to 6.1.3 in GR/T 1.1-2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
Ji will be replaced by our publishing center
Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net001. Describe the types and parts of host plants, plant products and other commodities that can carry or be found with pests. 6.6.2. Describe the symptoms or signs of hosts infested with pests, including typical characteristics and similarities and differences with other causes of symptoms. Attach slides, photos, diagrams and other image materials when necessary. 6.6.3. Describe the developmental stage of the detected pests, as well as the possible location and density of the pests on (in) the host plants, plant products and other commodities.
GB/T 23635--2009
6.6.4. Describe the host developmental stage, climatic conditions and seasons related to the pests. 6.6.5. Describe the detection methods of pests in the goods, such as using a diopter or a magnifying glass. Describe how to correctly collect and extract the pests from infected materials.
6.6.6. Describe the collection and extraction methods of pests from asymptomatic hosts or other materials (such as soil, water, culture medium). 6.6.7 Describe the activity of the detected pests. 6.6.8 Describe the sensitivity, specificity and reproducibility of all methods in this section, as well as the positive, negative control substances and reference substances used in the test. The differences from similar symptoms caused by other factors should also be provided. 6.7 Identification
6.7.1 One or more methods that can be used alone to identify pests should be provided. When there are multiple methods, the advantages and disadvantages of these methods should be given, as well as the equivalence of the methods when used alone and in combination. If several methods are required or there are multiple methods to alternately identify pests, a flow chart (table) should be drawn. 6.7.2 Methods in this section: mainly include methods based on morphological characteristics or morphological data characteristics, biological characteristics (such as toxicity or host range), and biochemical and molecular characteristics. Morphological characteristics can be obtained directly or after isolation and cultivation: if cultivation or isolation is a necessary step in the biochemical or molecular method, it should be described in detail. 6.7.3 For identification using morphological and morphological data characteristics, the following information should be provided: preparation of pests, mounting and inspection methods (such as optical, electron microscopy and measurement techniques); identification checklist (family, genus, species); a description of the morphology of the pest or its colony, including pictures of morphological identification characteristics and an explanation of the difficulty in observing specific structures; comparison of similar or related species; a relevant reference specimen or culture.
6.7.4 For each biochemical or molecular identification method (such as serological method, electrophoresis, PCR method, DNA barcoding method, RFLP method, IgG1 sequencing method), a detailed description (including equipment, reagents and consumables) should be given. References to related materials (such as standard substances and reagents to be used) should be attached after the method. 6.7.5 When more than one method can be used reliably, other appropriate methods should be listed as alternative or supplementary methods, such as: there are reliable morphological methods, but there are also appropriate biological methods. 6.7.6 The methods for isolating pests from asymptomatic host plants or plant products (such as detecting potential infestations) should be described in detail, and when necessary, biological methods for directly identifying asymptomatic materials should also be provided. Phytochemical or molecular methods. 6.7.7 Describe in detail the phenomena and problems that may arise in the identification of pests, and provide the contact method of the laboratory for comparison and verification of the test results when necessary.
6.8 Record and specimen sample storage
6.8.1 The record information that should be kept for pests identified by quarantine includes: a) the name of the identified pest;
b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d)
Description of the signs of damage or damage to the harmful crops (including relevant photos), and if there are no symptoms, it should also be stated: diagnosis Methods used for identification, including quality control materials, and results obtained by the identification methods: f
If morphological or morphometric methods are used for identification, relevant characteristics and measurement data, drawings or photos, and descriptions of the developmental stages of the pests shall be preserved; g
3) Enzyme chain reaction method.
4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results shall be preserved, such as: gel photos based on identification results or printouts of EI.ISA readout results; description of the extent of damage on the host (how many pest individuals are found, and the tissues and areas affected) , Statistics: i
j) Name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as alive or dead, the activity of the developmental stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or printed results of EL.ISA, etc.) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to GI3/T1.12000 6.3.8. 7 Basic contents of the preparation of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative appendix
This is an optional element. Abbreviated according to 6.4.1 in GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Prepared according to 6.1.3 in GR/T 1.1-2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
Ji will be replaced by our publishing center
Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net00The advantages and disadvantages of these methods, as well as the equivalence of the methods when used alone and in combination, should be given. If several methods are required or there are multiple methods to be used in combination to identify pests, a flow chart (table) should be drawn. 6.7.2 Methods in this part: mainly include methods based on morphological characteristics or morphological data characteristics, biological characteristics (such as toxicity or host range), and biochemical and molecular characteristics. Morphological characteristics can be obtained directly or after isolation and cultivation: if cultivation or isolation is a necessary step in the biochemical or molecular method, it should be described in detail. 6.7.3 For identification using morphological and morphological data characteristics, the following information should be provided: preparation of pests, mounting and inspection methods (such as optical, electron microscopy and measurement techniques); identification case list (family, genus, species); description of the morphology of the pest or its colony, including pictures of morphological identification characteristics and explanation of the difficulties in observing specific structures; comparison of similar or related species; related reference specimens or cultures.
6.7.4 For each biochemical or molecular identification method (such as serological method, electrophoresis, PCR method, DNA barcoding method, RFLP method, IgG1 sequencing method), a detailed description (including equipment, reagents and consumables) should be given. References to related materials (such as standard substances and reagents to be used) should be attached after the method. 6.7.5 When more than one method can be used reliably, other appropriate methods should be listed as alternative or supplementary methods, such as: there are reliable morphological methods, but there are also appropriate biological methods. 6.7.6 The methods for isolating pests from asymptomatic host plants or plant products (such as detecting potential infestations) should be described in detail, and when necessary, biological methods for directly identifying asymptomatic materials should also be provided. Phytochemical or molecular methods. 6.7.7 Describe in detail the phenomena and problems that may arise in the identification of pests, and provide the contact method of the laboratory for comparison and verification of the test results when necessary.
6.8 Record and specimen sample storage
6.8.1 The record information that should be kept for pests identified by quarantine includes: a) the name of the identified pest;
b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d)
Description of the signs of damage or damage to the harmful crops (including relevant photos), and if there are no symptoms, it should also be stated: diagnosis Methods used for identification, including quality control materials, and results obtained by the identification methods: f
If morphological or morphometric methods are used for identification, relevant characteristics and measurement data, drawings or photos, and descriptions of the developmental stages of the pests shall be preserved; g
3) Enzyme chain reaction method.
4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results shall be preserved, such as: gel photos based on identification results or printouts of EI.ISA readout results; description of the extent of damage on the host (how many pest individuals are found, and the tissues and areas affected) , Statistics: i
j) Name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as alive or dead, the activity of the developmental stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or printed results of EL.ISA, etc.) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to GI3/T1.12000 6.3.8. 7 Basic contents of the preparation of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative appendix
This is an optional element. Abbreviated according to 6.4.1 in GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Prepared according to 6.1.3 in GR/T 1.1-2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
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Learn Mathematics 11 Words
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http://foodmate.net00The advantages and disadvantages of these methods, as well as the equivalence of the methods when used alone and in combination, should be given. If several methods are required or there are multiple methods to be used in combination to identify pests, a flow chart (table) should be drawn. 6.7.2 Methods in this part: mainly include methods based on morphological characteristics or morphological data characteristics, biological characteristics (such as toxicity or host range), and biochemical and molecular characteristics. Morphological characteristics can be obtained directly or after isolation and cultivation: if cultivation or isolation is a necessary step in the biochemical or molecular method, it should be described in detail. 6.7.3 For identification using morphological and morphological data characteristics, the following information should be provided: preparation of pests, mounting and inspection methods (such as optical, electron microscopy and measurement techniques); identification case list (family, genus, species); description of the morphology of the pest or its colony, including pictures of morphological identification characteristics and explanation of the difficulties in observing specific structures; comparison of similar or related species; related reference specimens or cultures.
6.7.4 For each biochemical or molecular identification method (such as serological method, electrophoresis, PCR method, DNA barcoding method, RFLP method, IgG1 sequencing method), a detailed description (including equipment, reagents and consumables) should be given. References to related materials (such as standard substances and reagents to be used) should be attached after the method. 6.7.5 When more than one method can be used reliably, other appropriate methods should be listed as alternative or supplementary methods, such as: there are reliable morphological methods, but there are also appropriate biological methods. 6.7.6 The methods for isolating pests from asymptomatic host plants or plant products (such as detecting potential infestations) should be described in detail, and when necessary, biological methods for directly identifying asymptomatic materials should also be provided. Phytochemical or molecular methods. 6.7.7 Describe in detail the phenomena and problems that may arise in the identification of pests, and provide the contact method of the laboratory for comparison and verification of the test results when necessary.
6.8 Record and specimen sample storage
6.8.1 The record information that should be kept for pests identified by quarantine includes: a) the name of the identified pest;
b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d)
Description of the signs of damage or damage to the harmful crops (including relevant photos), and if there are no symptoms, it should also be stated: diagnosis Methods used for identification, including quality control materials, and results obtained by the method: f
If morphological or morphometric methods are used for identification, relevant characteristics and measurement data, drawings or photos, and descriptions of the developmental stages of the pests shall be preserved; g
3) Enzyme chain reaction method.
4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results shall be preserved, such as: gel photos based on the identification results or printouts of EI.ISA readout results; descriptions of the extent of damage on the host (how many pest individuals are found, and the tissues and areas affected) , statistics: i
j) Name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as alive or dead, the activity of the developmental stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or printed results of EL.ISA, etc.) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to GI3/T1.12000 6.3.8. 7 Basic contents of the preparation of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative appendix
This is an optional element. Abbreviated according to 6.4.1 in GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Prepared according to 6.1.3 in GR/T 1.1-2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
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Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net001. The records of pests identified by quarantine should include: a) the name of the identified pest; b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d) description of the signs of damage or damage to the harmful crops (including relevant photos), and the absence of symptoms should also be stated: the method used for diagnosis, including quality control materials, and the results obtained by the collection method; f) If morphological or morphometric methods are used for identification, save the relevant characteristics and measurement data, drawings or photos, and the description of the developmental stage of the pest; 3) Enzyme chain reaction method. 4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results should be preserved, such as: gel photos or ELISA readout printouts based on the identification results; description of the extent of damage on the host (how many pest individuals are found, the tissues and areas affected), statistics: i
j) The name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, provide a description of the status of the pest, such as live or dead, the activity of the development stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or ELISA printouts) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to 6.3.8 of GB3/T1.12000. 7 Basic content of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative Appendix
This is an optional element. Abbreviated according to 6.4.1 of GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Written according to 6.1.3 of GR/T 1. 1—2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
Ji will be replaced by our publishing center
Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net001. The records of pests identified by quarantine should include: a) the name of the identified pest; b) the number or reference number of the pest specimen (for traceability); the natural state of the infected material, including the scientific name if possible; the source of the infected cargo (including geographical location), and the place where the pest was intercepted or seized; d) description of the signs of damage or damage to the harmful crops (including relevant photos), and the absence of symptoms should also be stated: the method used for diagnosis, including quality control materials, and the results obtained by the collection method; f) If morphological or morphometric methods are used for identification, save the relevant characteristics and measurement data, drawings or photos, and the description of the developmental stage of the pest; 3) Enzyme chain reaction method. 4) Restriction fragment length polymorphism method.
Food Partner Area
GB/T23635—2009
h) If biochemical and molecular methods are used for identification, relevant documented test results should be preserved, such as: gel photos or ELISA readout results printouts based on the identification results; description of the extent of damage on the host (how many pest individuals are found, the tissues and areas affected), statistics: i
j) The name of the identification laboratory, including the name of the person in charge and (or) the person who identified it: k) Data and information on the collection, quarantine and identification of pest specimens; 1) If possible, a description of the status of the pest, such as live or dead, the activity of the development stage, 6.8.2 All evidence such as pest cultures, nucleic acids, specimens or test materials (such as gel photos or ELISA printouts) should be preserved.
6.8.3 The retention period of samples, specimens and records shall be specified according to actual needs: the retention period of general specimens and records shall be at least 1 year. 6.9 Normative Appendix
This is an optional element. The normative appendix gives additional clauses to the standard text according to 6.3.8 of GB3/T1.12000. 7 Basic content of informative supplementary elements
7.1 Ways to obtain information
The detection and identification procedures should provide ways to obtain pest quarantine, identification or other detailed information, including detailed contact information of experts, organizations, institutions, etc., so that relevant details in the methods can be consulted. 7.2 Informative Appendix
This is an optional element. Abbreviated according to 6.4.1 of GB/T1.1~2000. 7.3 References
The references are placed after the last appendix. The references should include relevant literature information resources cited for the preparation of detection and identification procedures.
7.4 Index
This is an optional element. Written according to 6.1.3 of GR/T 1. 1—2000. GB/T 23635-2009
Printing date: October 30, 2009
People's Republic of China
National Standard
Regulations on the preparation of procedures for the detection and identification of regulated pests
GB/T 23635:20C9Www.bzxZ.net
Published and distributed by China Standards Press
No. 16, Sanlihebei Street, Fuxingmenwai, Beijing
Postal code: 100045
Website spc.net.cn
Tel: 6852391668517544
Printed by Qindao Printing Factory of China Standards Press Distributed by Xinhua Bookstores in various places
Format 880×1230 1/16
Printing sheet 0. 75
Learn Mathematics 11 Words
July 2009 Edition July 2009 First Printing*
Book Number: 155066-1-38118 Price: 16.00 Yuan If there is any printing error
Ji will be replaced by our publishing center
Copyright exclusive Infringement will be investigated
Report phone: (010) 68533533
http://foodmate.net00
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