NY/T 1156.14-2008 Guidelines for Indoor Bioassay Tests for Pesticides Fungicides Part 14: Pot
Method for Control of Anthracnose in Cucurbits NY/T1156.14-2008 Standard Download Decompression Password: www.bzxz.net This part specifies the pot method for testing fungicides for control of anthracnose. This part applies to indoor bioassay tests of fungicides for pesticide registration against anthracnose in cucurbit crops.

ICS65.100
Agricultural Industry Standard of the People's Republic of China
NY/T 1156.14—2008
Guideline for laboratory bioassay of pesticidesPart 14: Potted plant test for fungicide control anthracnose [Colletotrichumorbiculare (Berk.&Mont) Arx) on cucurbits2008-05-16 published
2008-07-01 implemented
The Ministry of Agriculture of the People's Republic of China published
"Guidelines for Indoor Bioassay Tests of Pesticides and Fungicides" is a series of standards: Part 1: Test for inhibition of spore germination of pathogenic fungi - concave slide method; Part 2: Test for inhibition of mycelium growth of pathogenic fungi - flat blood method; Part 3: Test for inhibition of cucumber box sphaerocephala - flat leaf method; Part 4: Test for control of powdery mildew - pot stack method; Part 5: Test for inhibition of rice leaf slime - broad bean leaf method; Part 6: Determination of the combined effect of mixtures; Part 7: Test for control of cucumber frost disease - pot method; Part 8: Test for control of rice cancer disease - pot method; Part 9: Test for inhibition of gray mold - leaf method! Part 10: Pot method for control of gray mold; Part 11: Pot method for control of powdery mildew of melon; Part 12: Late blight test method; Part 3: Leaf method for control of late blight; Part 14: Anthracnose test method for control of melon; Part 15: Pot method for control of wheat leaf rust; Part 16: Turbidimetric method for control of bacterial growth; This part is Part 14 of the Guidelines for Indoor Bioassay of Pesticides. This part is prepared and managed by the Ministry of Agriculture of the People's Republic of China. The unit responsible for the preparation of this part is the Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this part are Yuan Shanluan, Zhu Chunli, Xu Yiping, Wu Xinping, Zhang Wei, and Yang E. NY/T 1156.14—2008
1 Scope
Guidelines for indoor bioassay tests for pesticides, fungicides NY/T 1156. 14---2008
Part 14: Test for the control of anthracnose in cucurbits: pot method This part specifies the test method for the determination of anthracnose by fungicides by pot method. This part is applicable to the indoor bioactivity determination test of fungicides for registered pesticides against anthracnose in cucurbit crops 2 Instruments and equipment
General laboratory routine instruments and equipment.
2.1 Electron ionizer (capacity 0.1mg).
2.2 Instruments.
2.3 Climate chamber.
2.4 Pipette or pipette, etc.
3 Reagents and materials
The reagents used in the method, if not specified, are of analytical grade; water is distilled water. 3.1 Biological test materials
The test pathogen is a wild sensitive anthracnose fungus of melons, Colletotrichumorbiculare (Berk. & Mont.) Arx strain. Record the source of the strain.
The test crop is a variety susceptible to gray disease: potted, cultured to 2 to 4 pieces of true green, numbered for standby use. 3.2 Test agent
Original drug (or elixir).
3.3 Control agent
Use the original drug (or mother drug) that has been registered and commonly used in production, and its chemical structure type or mode of action should be the same or similar to that of the test agent. www.bzxz.net
4 Test steps
4.1 Preparation of spore suspension
The test pathogen is cultured on a suitable culture medium. After spores are produced, the spores are washed with sterile water and filtered through 2 to 4 layers of gauze to prepare a suspension with a concentration of 1×105 spores/mL for standby use. 4.2 Preparation of the agent
Water-soluble agents are directly dissolved and diluted with steamed stuffing water. Other agents are dissolved in suitable solvents (methanol, acetone, methyl sulfoxide or dimethyl sulfoxide, etc.), and diluted with 0.1% leaf temperature-80 or other suitable surfactant aqueous solution. According to the activity of the agent, 5 to 7 series of mass concentrations are set, and the final content of organic solvents generally does not exceed 0.5% to 1%: the preparation can be directly diluted with water. 4.3 Treatment with the agent
Spray the liquid on the leaf surface until the gold part is moist, and wait for the axil of the drug to be naturally blown away by the wind for use. Each treatment has 3 pots, 4 times, and a control treatment containing only solvents and surfactants but not active ingredients and water is set. 4.4 Inoculation and culture
NY/T1156.14—2008
Use a microsampler to draw 10 spore suspension and inoculate it on the leaf. Each treatment should have no less than 30 inoculation points. For protective tests, inoculate 24 hours after the treatment with the drug; for therapeutic tests, inoculate 24 hours before the treatment with the drug. For disease resistance activation tests, inoculate again 3 to 7 days after the drug treatment. After inoculation, move to a moisturizing box (relative humidity 95%-100%) and culture in the dark for 24 hours, then culture at 22℃:~-27℃, light intensity 20000L1x, and humidity 80%~90%. 4.5 Investigation
When the morbidity rate of the blank control reaches more than 50%, use a vernier caliper to measure the diameter of the lesion once by the cross-cross multiplication method. Take the average value in meters (mm).
5 Data statistics and analysis
5.1 Calculation method
Based on the survey data, calculate the control effect according to formula (1) and express it as a percentage (%). The calculation result is retained to two decimal places. P=De=×100:
——control effect, unit is white fraction (%); blank control lesion diameter, unit is millimeter (mtm); D
difference of lesion treated with the agent, unit is millimeter (mm). 5.2 Statistical analysis
Use standard statistical software such as DPS (data processing system), SAS (statistical analysis system) or SPSS (systematic program for social sciences) to conduct regression analysis on the numerical value of agent concentration and the probability value of efficacy, calculate the ECs, E9 and other values ​​of each agent and their 95% confidence limits, and conduct difference analysis between the treatments of each agent
6 Results and report writing
Analyze and evaluate based on the statistical results, and write a formal test report.
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