This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of naked carp of Qinghai Lake. This standard is applicable to the detection and identification of germplasm of naked carp of Qinghai Lake. SC 1063-2003 Naked carp of Qinghai Lake SC1063-2003 Standard download decompression password: www.bzxz.net

Chapter 3 and Chapter 6 of this standard are mandatory, and the rest are recommended. This standard is issued by the Fisheries Department of the Ministry of Agriculture. This standard is issued by the Yangtze River Fisheries Research Institute of the China Academy of Fisheries Sciences. The main drafters of this standard are Shi Yanquan, Qi Gong, Qiao Jianxin, Tu Lehui, Hui Huihe, Liu Nianqin, Mao Yuefeng SC1063-2003 1 Scope Naked Carp of Qinghai Lake SL1063-2003 This standard gives the status of the sea tide [ Gonypseati (Kesler_) of the main ecological characteristics, growth and reproduction, genetic characteristics, and detection methods of this standard are used for the inspection and development of fresh germplasm. 2 Normative references || tt || The following documents are cited as references to this standard. All subsequent revisions (excluding the contents of the revision) or sub-editions are not applicable to this standard. Before the revision period, the parties to this standard shall reach an agreement on the Please note that the latest versions of these documents shall apply. For undated documents, the latest versions shall apply. H118854.1 Quality control of other species in aquaculture - Part 1: Testing methods GB/T18651.2 Testing of germplasm of aquaculture species - Part 2: Extraction methods GB/T18654.3 Quality control of farmed fish - Part 3: Determination of release factors GH/T1S651.12 Quality control of farmed fish - Part 1: Testing methods for free-range components SC1 0172000 Postal
3 Scientific name and classification
3.1 Scientific name
Royal fruit [yrocytris preatski (Kegsler) 3.2 Aliases
3.3 Classification
Genus Cyprinifornee (Cyprinifornee), Cyprinidae (Cyprinidae), Schizothoracinac (Schizothoracinac), GymlotypFis Gunther).
4 Main deformation and structural characteristics
4.1 External morphological characteristics
4.1. Appearance
This fish is long, with side flexures, flat surface, mouth proximal to the inferior position, slit-shaped, narrow lip, and interrupted lip opening groove. There is no stubbornness, the fish body surface is sparse and has a variety of specialized patches on both sides of the liver gate and the governor meridian. The back of the body is yellow or yellow, and the color of the gate changes to light yellow. There are irregular super-colored patches on the sides of the body, and some fish are all yellow. The appearance of the sea cucumber is shown in Figure 1.
4.1.2 Snake characteristics
4.1.2.1 Dorsal standing diagram; D, 1i-7(6-~9). 4. 1.2.2 Instantaneous screening type: A, 1i-3.
4.1,2.3 No. .. Issue number: outer 13~13, inner 21~184.1.3 Verifiable traits
For the individual with body length 21cm-3ct. and weight 100--450g, the actual disc ratio values ​​are shown in Table 1SC10632003
Total length/body length
Body length/body height
1. 18: -0. 519: 5. 88---. 344.2 Internal structural characteristics
4, 2. 1 Yi
The outer shape of Qinghai naked carp
The measurable trait ratio values ​​of the sea lake operation
The original length, length
5. uu. 04
5. u4 1 :. :0
Two chambers, the rear chamber is 1.69-3.3 times the length of the front chamber, the wall 4.2.2 lower teeth
two lights: 3-4/4-3.
4.2.3 Vertebra
Total number of general push books: 17.~52.
4. 2. 4 Peritoneum
The length of the joint is 1.11 times~5.2 times the body length,
Growth and breeding
5.1 Growth
The total length and weight of fish in different groups are as follows? , mile length/limit test
5.66 :.V
length body height
The total length and body height of different age groups are measured in Table 2
: 22. 33-
1.860:0.1E
30, t3!
year static/identification
x ​​Upper Valley
F,16±
54,251
cyst 2 (ammonium)www.bzxz.net
76, E ±
juice 1:X one is done, the average low sample self-certification sample plus the identification difference, let the 2 samples 100 years
5. 2 need to breed
sexual maturity years, female value 4 or above, single fish 3~4 years old. 5.3.1
Origin type: Sexually mature individuals ovulate once a year and lay in batches: 5.2.3
Egg number: The fertility rate of individuals in different age groups is shown in Table 3. 265.
Table 3 Average fertility rate of broodstock individuals in different age groups/
Absolute fertility rate/
Relative fertility rate/(weight/% ​​body weight)
Genetic characteristics
6.1 Cytokine characteristics
Body color number: 2\=92;
Karyotype formula: 24-26sm+26s-1et
Body type is shown in Figure 2.
Staining rough map
84, 5c
415,25
SC 1063—2003
583, 0
5=1,5±
IIG, 21
64E, 9±
SC1063—2003
6.2 Biochemical characteristics
The group lactate dehydrogenase was electrophoresed with 1 enzyme (T.1H) as shown in Figure 3. The enzyme band was drawn in Figure 4. The activity of the acid band is shown in Table 4.
.1H: :
Strong activity, high yield
Detection method
7.1 Sampling rules
11 1.1>H :
Figure 3 Isoenzyme electrophoresis of renal tissue
Scanning diagram of LDH isoenzyme bands in renal tissue
Activity intensity of LDH isoenzyme bands in renal tissue
According to GE/71853-.2:
7.2 Character determination
According to GB/T18654.3:
7.3 Determination of age
According to GB/T18654.3,
7.4 Determination of fertility
According to S:10372.
7.5 Chromosome detection
According to GB/T18654.12.
7.6 Isocyanate determination
SC1063—2003
7.6.1 Sample preparation: add 20% dextrose to the sample in a ratio of 1:5, mix well in an ice bath, centrifuge at 4,12930r/1 for 30min, and store the supernatant in a refrigerator for later use. 7.6.2 Electrophoresis separation: use a horizontal plate electrophoresis instrument and a 7.5% olefin gel for separation, the electrode is rinsed with a trimethylolpropane (S)-amino acid system (I8) electrophoresis paint, and the electrophoresis is carried out at a speed of 30.0-150°C. The amount of electrophoresis is 100%.
Staining: After the electroporation, prepare the mulberry color solution (see the formula of the staining solution in the table) in a constant temperature of 30°C. Table 5 Staining solution formula
1.01 μl Tri-HCl color solution (>El-E. 3>) 1 mL
Note: IICL is saline,
Spectrum
Digital
Digital
Digital
7.6.4 Scanning determination: Use a laser scanner to scan the electrophoresis spectrum. 7.7 Detection and determination
According to the provisions of CB, T16,1,
1 mzl/1. Lactic acid
(ll-7, 5)/ml
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