Pesticide-Guidelines for the field efficacy trials(II)-Part 125:Herbicides against weeds in soybean
Some standard content:
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National Standard of the People's Republic of China
GB/T17980125—2004
Guidelines for Field Efficacy Tests (II)
Part 125: Herbicides against weeds in soybean fieldsPesticides
Guidedines for Lhe fhell efricaty Lrials t I )-Part 125:Herbicides against weeds in soybean fields 90yhear2004-03-03 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration
2004-08-01 Implementation
GB/T17980.125--2004
The efficacy test is one of the important contents of pesticide registration in my country, and is the important technical basis for formulating pesticide product labels. Labels are the key to the safe use of pesticides. In order to standardize pesticides, the efficacy test is the content of the law, so as to make the efficacy test report of my country more recognized by the world and to align with international standards. The standard is a standard issued by the European and American Protection Organization for the Protection of Agricultural Products (EPP) and the European and American Agricultural Products Protection Organization (EPO). It is a standard for the average area of the agricultural products in China. It is formulated based on the actual situation in my country and through a large number of daytime tests: the majority of crops will be harmed by weeds during the entire growth period, causing serious damage to crops. In production, weed control should be carried out with herbicides. In order to determine the light action tolerance of herbicides for controlling weeds in soybean fields, or the appropriate application period, and the safety of soybean pesticides, and to provide a basis for the efficacy evaluation and safe use of registered soybean pesticides, this part of GB/17JU was formulated. This part is one of the series of standards for pesticide efficacy test rules (:), but it is an independent part of this part. This part is compiled by the Agricultural Science and Technology Bureau of the People's Republic of China. Department responsible for drafting: This part is drafted by: Institute of Pesticide Control, Ministry of Agriculture. The main drafters of this part are: Wei Tongxiang, Chen Tiebao, Hui Fuping, Zhou Wei, Liu Xue, Gui Guanbiao, Gao Huoli. The Institute of Pesticide Control, Ministry of Agriculture is responsible for the interpretation of this part. 428
1 Scope
Criteria for field efficacy test (II)
Part 125: Herbicides for controlling weeds in soybean fields (GB/T17980.125-2004
This part specifies the method and basic requirements for the plot test of herbicides for controlling weeds in soybean fields (GB/T17980.125-2004). Part 125 specifies the method and basic requirements for the plot test of herbicides for controlling weeds in soybean fields. Part 125 applies to the registration of herbicides for controlling weeds in soybean fields and the evaluation of drug sensitivity. Other micro-tests are carried out according to the technical requirements.||tt ||2 Experimental conditions
2.1 Crops and cultivars
Record the cultivar type of soybeans, select the common varieties and varieties that are widely planted. Obtain the name of the soybean variety. 2.2 Selection of test weeds
The plots should have representative weed populations. The density of the main weeds should meet the test requirements, and the distribution should be consistent with the weed control spectrum of the test weed control (e.g. single weed and/or double weed, annual and/or perennial). Record the Chinese name of each weed and change it to Latin name:
2.3 Cultivation conditions
All test plots should be planned and planned (soil type, organic matter content, pH value, soil fertility, tree planting and cultivation, and should comply with the current scientific agricultural practice: GAP). The cultivation measures such as soybean sowing depth, row spacing, etc. should be in line with the actual production of the pond. Record the crop and the pesticides used before, and avoid selecting plots for plot tests that have been treated with herbicides that have an effect on soybeans. If necessary, record the following: 3 Experimental design and safety selection 3.1 Pesticides 3.1.1 Test Pesticides Trade name, Chinese name, generic name, dosage form, manufacturer of the test pesticide: The test pesticide treatments should be set at high, medium, low and medium doses (the setting is to evaluate the safety of the test pesticide on soybeans) or the pesticides specified in the agreement (the test design agreed upon by the test entrusting party and the test contractor). 3.1.2 Control pesticides should be registered products with good results in practice. The type and effect of the control pesticide should be similar to those of the test pesticide. The dosage and treatment method should be used appropriately. Special conditions can be regulated by the test date. If the test agent is a pesticide, the total amount of the test agent should be adjusted. 3.2 Plot arrangement
3.2.1 Plot arrangement
The test plots are arranged in groups. In some cases, special conditions can be arranged by neighboring Liufu. For example, in the case of multiple control trials, in order to avoid the interference of multiple distributions, it is necessary to use the corresponding arrangement rules according to the actual situation.
3.2.2 Plot area and weight
Plot type: 20m--30m (not less than 3 rows). The area of the test product is 10m*-15.425
GB/T17980.125-2004
Repeat the source, at least 4 times.
3.3 Application methodwwW.bzxz.Net
3.3.1 Use method
Follow the instructions on the label or the contract requirements. Generally, the spraying should be in accordance with the scientific agricultural practice. 3.3.2 Equipment used
The spray equipment should be able to evenly distribute the pesticide to each plot and ensure the dosage of the pesticide. The spray nozzle of the spraying agent should be used with the sprayer and the spraying agent should be specified. Record the sprayer type, nozzle model, spraying force, spraying depth, etc. If necessary, record the spraying method and depth. 3.3.3 Application time and frequency. According to the characteristics of the pesticide, the application time can be divided into: B) before sowing (with or without soil digging) L) after sowing (with or without soil digging) C) after seedlings: D) after seedlings, record the application time, the growth period of weeds and crops, the main weed species and the maximum application temperature, the lowest temperature, wind speed, soil dryness, rainfall, etc. The application time and frequency are generally in accordance with the regulations on the label. The test agent should be used according to the label or in accordance with the regulations, and the dosage is expressed in g/bt of the effective ingredient and the dosage is expressed in 1.m\. The meteorological conditions for pesticide application should be adjusted according to the characteristics of the pesticide and the conditions of the pesticide application machine. The conclusion should be as follows (except for special requirements):
3.3.5 Data requirements for pesticides used to control pests and non-specific weeds If other pesticides are required, the pesticides that have a great impact on the test pesticide, the target and the crop should be selected and separated from the test pesticide and sprayed on all plots to minimize the interference of these pesticides and record them accurately (such as pesticide name, application period, etc.).
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4. 1. Meteorological data
Meteorological data for the entire test period should be obtained from the test site or the nearest meteorological station, such as rainfall (rainfall type, expressed in mT), average temperature, maximum temperature and minimum humidity (expressed in mT), wind speed, rainfall, sunlight and relative humidity. For example, the meteorological data on the day of application and one day before and after the application are the adverse weather conditions that may affect the test results during the test period, such as severe or long early rains, heavy rains, etc. The following requirements must be recorded: 4.1.2 Soil data
Record soil type (record its composition as much as possible), organic matter content, soil pH H value, large filling degree (such as ten-wet type, waterlogging) and research 4.2 Field management data
Record samples of land preparation, watering and fertilization, etc.,
4.3 Survey method, time and frequency
4.3.1 Weed survey
Detailed description of the shape of weeds (such as cow-weed type, time-slightly shaped, etc.): Clearly explain the mode of action of the drug, record the number of weeds in the plot, the degree or weight of weeds, which can be expressed in absolute or percentage terms. 4.3.1.1 Absolute value (number measurement) survey method
GB/F 1798D.125—2004
Calculate the number of each weed and take the effective amount: conduct a survey on each plot. You can also randomly select 3 points in each plot, and then select 0.25m~1 square to quickly retreat
4.3.1.2 Estimation Visual survey method
4 treatment plots in the same block group are compared to estimate the weed control. This survey method includes estimating the total annual weed control. The estimation should take into account the weed quantity, coverage, height and seedling density (i.e. the actual weed control). In principle, this estimation method is fast and simple, and the results can be expressed in terms of percentage, or converted to express the weed control percentage effect (0 is the constant). No control effect, 100 heads for complete weed control, and the absolute effect of the untreated plot on the control.
In order to overcome the difficulty of accurately estimating the difference and using homogeneous variance, the following levels can be used for investigation: 1: No weeds:
Level 2: The number of weeds in the control area is 2.6%~4, which is equivalent to 5.15--10% of the weeds in the indoor area; , the blank area is the same as the control area, the first level is 25.1%~35% of the blank area, the second level is 35.1%~10% of the blank area, and the third level is 37.6%~10% of the blank area. The classification range can be used directly without converting it into an estimated half-score.
No matter which kind of adjustment is used, the method of action of the product should be clearly stated. The common symptoms of damage (growth inhibition, head, glassy shape, etc.) should also be accurately described.
4.3.1.3 Crop Observation
) The screening results should be detailed and the affected crops should be described accurately. The symptoms of delayed emergence, seedling death, growth inhibition, fading, clear type, contact spots, poor adhesion, and death of cattle should be described accurately. ) Selective tests should be carried out in different areas to measure yield.
The safety of the test pesticide should take into account the interaction between the pesticide and the cultivation measures, lodging, fullness, and abnormal micro-conditions.
4.3.1. 4 Side effects
The impact of the pesticide on some reptiles,
4.3.2 Time and frequency of inspection
Unless otherwise specified in the manual, the pesticide safety inspection time should be coordinated with that of the pesticide as much as possible. :) After sowing, the first inspection should be conducted by the former 2-piece inspection team: observe the emergence of soybean seedlings and the emergence of weeds, and the second inspection should be conducted during the 2-piece compound leaf period of soybeans, and the Donglihan weed control effect (number measurement) should be conducted. At the same time, the second inspection of the pesticide should be conducted by the former 2-piece inspection team: before the second inspection, the number of weeds remaining should be observed, and the weed control effect (number and effect) should be observed. The fourth inquiry is to check whether the target drug matures quickly and test the residual weeds (weeding effect) on the day of application. b) After application, check the percentage of each weed before application and adjust the percentage of each weed after the first inquiry. Check the residual weeds effect and check the drug for the time being. Before the second period of growth or when the amount of weed control is better, the absolute value method is used to investigate the weed control effect (the number of surviving plants and fresh weight in the soil). The third investigation is before harvest. It is necessary to delay the growth of crops. 15: GB/T 17980.125-2004 Crop yield investigation The experiment should be divided into different areas for by-product production. When the by-product is left, the middle section of the by-product area should be used, and the yield area and 15m-155cm should be measured. The data should be processed with appropriate statistical methods: write a report, explain the statistical methods used, and analyze the results. Put forward conclusive points of application effect evaluation (product characteristics, application technology, efficacy, pesticide damage) and economic benefits (increase in production, increase in efficiency, quality, cost).
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