This standard was first issued in January 1994. This standard is a revision of GB/T 14926.8-1994 "Test Methods for Mycoplasma in Laboratory Animals". This standard specifies the test methods for mycoplasma in laboratory animals. This standard is applicable to mycoplasma detection in mice and rats. GB/T 14926.8-2001 Test Methods for Mycoplasma in Laboratory Animals GB/T14926.8-2001 Standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
CB/T 14926. 8-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T 14926.82001
Revised GB/T11926.8-199 Laboratory animal test method for mycoplasma.
This standard specifies the growth control test for certain mycoplasma species, extends the blind transmission test to 1, and adds ELISA as an applicable method.
This standard is issued by the Ministry of Science and Technology of the People's Republic of China and the drafting unit of this standard is the Chinese Society of Laboratory Animal Science. The main drafter is Li Hong.
This standard was issued by the Ministry of Science and Technology of the People's Republic of China and the drafting unit of this standard is the Chinese Society of Laboratory Animal Science. The main drafter is Li Hong.
This standard was issued by the Ministry of Science and Technology of the People's Republic of China and the drafting unit of this standard is the Chinese Society of Laboratory Animal Science. The main drafter is Li Hong.
This standard was issued by the Ministry of Science and Technology of the People's Republic of China and the drafting unit of this standard is the Chinese Society of Laboratory Animal Science. This standard limits the actual shoe main body in the test ancient method. This international standard is applicable to small program points. 2 Reference standards. The original exceeds the following standards. All standards are valid. All standards are used. CE/T1192#42001 Implementation number GB/T1492C9 GB/T149H 3 Principles of experiment. The main purpose of the experiment is to ensure the safety of the vehicle. 2001 3 stores in the main time
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42 real parts show active grab
4.3 spiral star for the instrument
4.4 capillary pipette, each sample 1
45 constant temperature water box.
46 high continuous centrifuge.
47 ultrasonic drum phase powder Korea device,
48 follow the stop
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GB/T14926-8--2001
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accompany in the hospital body start culture is and stop culture medium can be carried out disease phoenix special ink development, respect the animal with support original with animal natural after serum antibody basic level low,
please only use for the step detection of the selected 4.9 B wedding plate, 40 wells.55 wells oh 96 wells ( 3. Remove or not remove, use bacteria to select clean overnight, light exposure for 1h4.10 micropipette, volume 5-50L and 200ml 5.1 Proofing culture medium for mycoplasma infection 2001-08-29 China National Quality Inspection and Quarantine Bureau 2002-05-01 Standard 5.2 Thick solid culture medium for mycoplasma infection 5.3 Solid culture medium for mycoplasma infection 5.4 Din staining night 5.51 Oxygen culture: Mycoplasma spp., Mycoplasma spp. 5.5.2 The inoculated cells were centrifuged at 1000/min for 30min under the same conditions. 5.5.3 The C-sediment was sonicated with PBS and the supernatant was the ELISA antigen. The ELISA was performed with ELISA-labeled goat or rat IgG antibodies, and the corresponding animal blood was prepared by hand to obtain the peroxide-labeled spherical protein A (SPA2). 5.7 The serum of mice was used for mycoplasma immunostaining. 5.8 Open the active clear
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61 Isolation culture method
61.1 Model process room
6.1.2 Operating apparatus
6.1.2.1 Sample
GB/T14926-8—2001
Main Mo Wei car Bacillus nucleus culture medium
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Pot Irradiated Bacillus standard culture medium
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6.1-2-2 body away from the wall culture
technique GB14825.42-20014.18 in accordance with the inoculation of scattered C36 ± 13C incubator culture. 6.12.3 treasure
mycoplasma companion in semi-fluid culture medium for 3 to 7 years, the transformation of the tiger on the culture medium can be a small book, treasure or sand grains of the country.
b) Transfer about 0.5mL of the semi-fluid culture medium of the bacteria to the mycoplasma culture medium, incubate at 36±13°C for 5-7d, then transfer 1mL of the culture medium to the mycoplasma solid culture medium, mix well with L-spray, and place the culture medium in a fresh-keeping medium. 2
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GB/T 14926.8--2001
“) Mycoplasma may form “egg-like” or “flake-like” colonies after 3 days of culture on solid culture medium. d) Based on color, the solid culture medium with suspicious colonies shall be placed flat for staining. After 30 minutes, the mycoplasma colonies will become more colorful and the surrounding space will be colorless.) If no mycoplasma is found after 7 days of culture, about 1/10 of the culture medium shall be taken out and inoculated into the same culture medium for surface culture. If no measurable colonies are found after 7 days of culture at (36±1)C, it is considered positive: for those with measurable colonies, make a family photo. b) .c>d) Explosion determination.
6.2 High immunosuppression adsorption test (ELISA)
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Add the appropriate amount of the root detection solution, select the antibody solution, and place 100L in each well. Place &TC point in the center. 62-2 Use special training water to add 5ml water, each for 3min, 6-2-3 Add the sample
Replace the serum and the test serum with a direct release device, and use the appropriate amount of alcohol to release the enzyme solution in well 100971. 6.2.4 Add the sample solution and select the enzyme solution, and add 1ml water to each well. 00, 31h, select the same as above 6.2.5 Add the appropriate amount of liquid to each well and place it at 37 °C for color development for 10~15m62.6 Do not add the appropriate amount of liquid to each well and measure the A value on the fermentation mark and read the A value from each well at 4 positions. 6.2.8 Result determination Under the condition of negative and positive library clearing or independent conditions, determine the result. 6.2.8.1 If the following two conditions are met at the same time, the positive a value of the special test should be lowered to 202 b) Select the value of the test serum/ If the A value of 2.1.62.8-2 does not meet the above 8 conditions, it is judged as closed. If there is 1 case recommendation, it is considered to be correct and needs to be retested. If the positive result is positive, it is judged to be bright positive. The young fruit eye fragrance
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