This standard provides the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of silver carp (Hypophthalmichthys molditrix). This standard is applicable to the germplasm identification of silver carp. GB 17717-1999 Silver carp GB17717-1999 Standard download decompression password: www.bzxz.net

GB17717—1999
This standard is formulated to identify silver carp, protect and preserve the excellent traits and germplasm of silver carp original species, avoid germplasm mixing and degradation during seed production, and carry out effective monitoring of silver carp germplasm. This standard is mainly the result of the "Eighth Five-Year Plan" scientific and technological research, and absorbs the relevant results of the "Sixth Five-Year Plan" and "Seventh Five-Year Plan" scientific and technological research. Appendix A of this standard is a prompt appendix.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the Freshwater Aquaculture Sub-Technical Committee of the National Technical Committee for Aquatic Standardization. The drafting units of this standard are: Shanghai Fisheries University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard are: Li Sifa, Zhao Jinliang, Cai Wanqi, Zhou Biyun, Lv Guoqing, Fan Zhaoting, Xu Zhongfa. This standard shall be implemented from July 1, 1999. 91
1 Scope
National Standard of the People's Republic of China
Silver carp
GB17717-1999
This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of silver carp (Hypophthalmichthys molitrix).
This standard applies to the germplasm identification of silver carp.
2 Cited Standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard was published, the versions shown were valid. All standards are subject to revision, and parties using this standard should explore the possibility of using the latest version of the following standards. GB17716—1999 Silver Carp
3 Name and Classification
3.1 Scientific Name
Silver Carp (Hypophthalmichthys molitrir). 3.2 Classification position
Belongs to the order Cypriniformes, the family Cyprinidae, the subfamily Hypophthalmichthyinae, the genus Hypophthalmichthys.
4 Main morphological and structural characteristics
4.1 External morphological characteristics
4.1.1 Appearance
The body is flattened laterally and slightly high, with an abdominal ridge between the lower part of the pectoral fin and the anus. The head is relatively large, about one-fourth of the body length, with a short, blunt and round snout, a large and oblique mouth, and a slightly upward tilted lower jaw. The eyes are relatively small and located below the lateral midline of the body. The scales are small and the lateral line is complete. The gill rakers are specialized and connected to each other to form a spongy sieve membrane. There are suprabranchial organs. The tail fin is deep. The end of the pectoral fin reaches or slightly exceeds the base of the pelvic fin. The body is silvery white, the back is gray, and the edges of the dorsal and caudal fins are slightly black. The external morphology of the silver carp is shown in Figure 1.
4.1.2 Countable traits
4.1.2.1 Dorsal fin type: D3.6～7.
Anal fin type: A3.11~14.
Lateral line scale type: 97
26～27
16～18
Approved by the State Administration of Quality and Technical Supervision on April 5, 199992
Implemented on July 1, 1999
4.1.3 Quantifiable traitsWww.bzxZ.net
GB 17717-1999
Figure 1 Appearance of silver carp
For individuals with a body length of 21.8~91.0cm and a body weight of 180~19500.0g, the proportion values ​​of the measured traits are shown in Table 1. Table 1 Measured measurable traits ratio
Body length/body height
Total length/body length
Body length/head length
1Head length/interocular distanceBody length/tail peduncle length
Brain length/eye diameter
Head length/snout length
1.170±0.0343.349±0.1933.840±0.246|3.619±0.4808.443±1.5521.936±0.1558.296±0.8964.2 Internal structural characteristics
Two chambers, the anterior chamber is long and expanded, the posterior chamber is small and conical. 4.2.2 Hypopharynx teeth
Flat fan, tooth surface feather-shaped. Tooth formula 4/4.
4.2.3 Vertebrae
Total number of vertebral hips: 40~42.
4.2.4 Peritoneum
Black.
5 Growth and reproduction
5.1 Growth
Caudal peduncle length/caudal peduncle height
1.127±0.123
The measured values ​​of body length and weight of silver carp of different age groups are shown in Table 2. The growth equation and the relationship between body length and weight of fish of different age groups corresponding to Table 2 are shown in Appendix A (suggested appendix). Table 2 Measured values ​​of body length and weight of fish in different age groups Age\, age
Measured body length
Measured weight
Measured body length
Measured weight
31.9~52.6
495~2400
30.3~53.5
500~2800
45. 5~61. 2
1715~4450
38. 3~62.7
900~4 250
1) Age is mainly identified based on the number of annual rings on scales. 3+
45. 7~67. 0
1 920~6 800
54.0~68.5
3 000~6 800
54.0~74.5
4 400~7500
53.3~76.0
2500~~9 500
63.5~79. 2
4 700~9 500
66.5~~84. 0
81.0~91.0
6500~1250011000~12500
5.2 breeding
GB 17717 -—1999
5.2.1 Age of sexual maturity: Female fish are 3 to 5 years old, male fish are 2 to 4 years old. Sexually mature individuals mature their gonads once a year and spawn once a year. 5.2.2
5.2.3 Egg carrying capacity: See Table 3 for the egg carrying capacity of individuals in different age groups. Table 3 The number of eggs in individuals of different age groups
Age, age
Weight·g
Absolute number of eggs, grains
Relative number of eggs, grains/g
Genetic characteristics
6.1 Cytogenetic characteristics
6.1.1 Chromosome number
5 713. 5±806. 7
(6. 22±1. 92)X105
110.6±36.2
Somatic cell chromosome number is 2 times, 2n=48.
6.1.2 Karyotype
5959.3±682.3
(6. 69±1. 35)×105
112.5±27.6
7321.4±894.4
(8. 63±1. 55) X105
119.7±30.9
10 pairs of mid-centromere chromosomes (m group); 9 pairs of sub-metacentric chromosomes (sm group); 5 pairs of subtelomeric chromosomes (st group), number of chromosome arms (NF) 86 (see Figure 2). 78
6.2 Biochemical genetic characteristics
Chromosome karyotype of silver carp
6.2.1 Lactate dehydrogenase (LDH) isozymes in muscle 6.2.1.1 The electrophoresis zymogram of lactate dehydrogenase (LDH) isozymes in muscle is shown in Figure 3. The enzyme band scanning diagram of lactate dehydrogenase (LDH) isozymes in muscle is shown in Figure 4.6.2.1.2
Figure 3 Electrophoresis zymogram of LDH isozymes in silver carp muscle LDHs
Figure 4 LDH isozyme band scanning diagram of silver carp muscle 6.2.1.3 The activity intensity of lactate dehydrogenase (LDH) isozyme bands in muscle is shown in Table 4.94
Activity intensity
GB 17717 -1999
Activity intensity of LDH isozyme bands in silver carp muscle
Population variation range: The proportion of polymorphic loci in silver carp is 11.8%~23.5%, and the average heterozygosity is 0.0484~~0.0591.6.2.1.4
Detection method
Age identification
According to the provisions of GB17716.
7.2 Determination of fertility
According to the provisions of GB17716.
7.3 Chromosome detection
According to the provisions of GB17716.
7.4 Lactate dehydrogenase (LDH) isozyme spectrum shall be in accordance with the provisions of GB17716.
GB 17717 --1999
Appendix A
(Suggested Appendix)
Calculated values ​​of body length and weight for different age groups, growth equation, body length and weight relationship formula Body length and weight calculated values
The average calculated values ​​of body length and weight for fish of different age groups corresponding to Table 2 are shown in Table A1. Table A1
Age, age
Deferred body length
Deferred weight
Deferred body length
Deferred weight
Growth equation
Female fish:
Male fish:
The average deferred values ​​of body length and weight of fish in different age groups1+
L 125. 6[1 - e-0. 160 7(+0. 08 4)W, =52 962. 6[1 — e-0. 160 7(+0 068 4)3L, -111. 1[1 -- e-0. 175 8(+0. 193 3)]W, = 31 766. 7[1 e-0.175 8(+0.193 3)]3Where: L. - fish body length at age t, cm; W, fish body weight at age t, g;
e-natural logarithm;
age of a fish.
A3 Body length and weight relationship
Fish stage
Fish body weight, g;
Where: w-
L—-fish body length, cm.
A3.2 Edible commercial fish stage
Broodstock stage
Female fish:
Male fish:
W = 0. 019 9 X L2. 943 3
W = 0. 012 5 × L3. 082 4
W = 0. 062 0 × L2. 7511
W = 0. 021 1 × L2. 949 7
（A1）
..（A4)
....(A5)
.........( A6 )
....(A7)
·（A8)
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