This standard specifies the detection method and reagents for rabbit hemorrhagic disease virus (RHDV). This standard is applicable to the detection of rabbit RHDV. GB/T 14926.21-2001 Detection method for rabbit hemorrhagic disease virus in experimental animals GB/T14926.21-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T14926.21-2001
Laboratory animals
Microbiological examination methods (4)
Laboratory animalMicrobiological examination methods2001-08-29Promulgated
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine
Implementation on 2002-05-01
GB/T14926.21—2001
This standard is a revision of GB/T14926-21-1994k "Detection method for immune-hemorrhagic disease virus in laboratory animals". In addition to the relevant contents of "5.1 Detection of immunoglobulin virus antigen\ and \5.2-2 ELISA detection of immunoglobulin virus antibody\, the result judgment standard in \5 Detection method and result judgment\ has been revised.
This standard was proposed and coordinated by the Ministry of Science and Technology of the People's Republic of China, and the drafting of this standard was signed by the Chinese Society of Laboratory Animal Science. The main drafter of this standard is He Zhengming
This standard was first issued in January 1994.
1 Model
National Standard of the People's Republic of China
Laboratory animal
Laboratory animal-Method for examination of rabbit hemorrhagic virus
disuasrusRnDV) This standard defines the exemption from
This standard is applicable to the test of exemption from RHE
2 Reference standards
The following standards are included in the text and are valid through the standard. All standards 5 have been revised and the use of the same customer limit design has become
GB/T14926
GB/T14924
3 Principle
Experimental momentum
Experimental animals
Hemagglutination inhibition test
Under the conditions of water, energy
According to RHDV
According to the RHDV antigen, the most effective
4. Reagents and equipment
4.1 Reagents
4.1-1 Hemagglutinin
Antibodies
Artificial infection or spontaneous infection
4.1-2 Positive serum
RHDV antigen immunization or natural infection recovery serum 41.3 Negative serum
No RHDV infection, no immunization serum. 4.1.4 Human\0\ type red blood cells,
42 Equipment
4 .2137C constant temperature incubator.
42-2 color shaker.
Micro acid reaction plate (U-type or V-type)
Micro pipette (capacity 5~50μL) or micro dilution stick 4-2-4
principle
GB/T14926.21-2001
replaces GB/T1492421-—1994
point clauses, when this standard was published, the versions shown are the latest versions of the standard.
test the HDV antigen in the immune system, or || tt||Can be used to detect RHV antibodies in serum.
Speed, 30gt/min for 10min The supernatant obtained was approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 2001 and implemented on May 1, 2002
Detection method
GB/T14926-21-2001
The HA method (see GB14926.53-2001) was used for virus antigen detection. The HAI method (see GB/T14926-54-2001) was used for blood testing. 5-2bzxz.net
6 Results judgment
6-1 For common delayed immunizations such as lumbar vaccination, the HAI antibody titer should be >1:106-2 For clean delayed immunizations, the first vaccination should not be carried out. The HAI antibody titer ≤1+10 is judged as positive. For positive (HAI antibody titer>1:10) test results, the same method is used for retesting. If it is positive, it is judged as positive. 6- The supernatant of the liver is changed to 10 before the new house. The HA concentration is 1+16 for positive. For positive (HA concentration -16 test results, the same method is used for retesting. If it is still positive, it is judged as positive. Result report
Make a report based on the judgment results.
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