GB 19442-2004 Technical Specifications for the Prevention and Control of Highly Pathogenic Avian Influenza
Some standard content:
All technical contents of this standard are mandatory. Foreword
GB19442—2004
This standard is formulated in accordance with the Animal Epidemic Prevention Law of the People's Republic of China and relevant laws, regulations and rules, with reference to the relevant parts of the International Animal Health Codes of the World Organization for Animal Health (OIE), and based on relevant scientific and technological achievements and practical experience. This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the National Technical Committee for Animal Quarantine Standardization. The drafting unit of this standard: National Animal Husbandry and Veterinary Station of the Ministry of Agriculture. The main drafters of this standard: Xu Baiwan, Li Xiufeng, Chen Guosheng, Zhang Jie, Wang Zhongli, Tian Guobin, Liang Quanshun, Li Kailun. 1 Scope
Technical Specifications for the Prevention and Control of Highly Pathogenic Avian Influenza
GB 19442--2004
This standard specifies the diagnostic technology, epidemic reporting, epidemic treatment, prevention and control measures, control and purification standards for highly pathogenic avian influenza. This standard applies to all units and individuals engaged in poultry breeding, management, production and management of poultry products, and animal epidemic prevention activities within the territory of the People's Republic of China.
2 Normative references
The clauses in the following documents become clauses of this standard through reference in this standard. For all dated references, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For all undated references, the latest versions apply to this standard. GB16548 Procedure for harmless treatment of diseased poultry and their products GB/T18936 Diagnostic technology for highly pathogenic avian influenza "Law of the People's Republic of China on Animal Epidemic Prevention" "National Emergency Plan for Highly Pathogenic Avian Influenza" (Guobanfa [2004] No. 12) "International Animal Health Code" 2002
3 Terms and definitions
The following terms and definitions apply to this standard. 3.1
Highly pathogenic avian influenza HPAI is a highly pathogenic virus of influenza A virus in the family Orthomyxoviridae. 3.2
epidemic spot
The location of the diseased poultry. Generally refers to the poultry farm (household) or other relevant slaughtering and business units where the diseased poultry are located; if they are raised in rural areas, the natural village should be designated as the epidemic spot.
epidemic area
The area within a radius of 3km with the epidemic spot as the center. When dividing the epidemic area, attention should be paid to the local breeding environment and natural barriers (such as rivers, mountains, etc.).
affected area
The area within a radius of 5km from the epidemic area.
4 Diagnosis
4.1 Epidemic characteristics
Chickens, turkeys, ducks, geese, quails, pheasants, partridges, ostriches, pigeons, peacocks and many other poultry are susceptible. The incubation period of this disease ranges from a few hours to several days, and can be up to 21 days. The main sources of infection are sick poultry and infected birds (including waterfowl and migratory birds). The virus is mainly transmitted through contact with infected poultry and their secretions and excrement, contaminated feed, water, egg trays (boxes), bedding, breeding eggs, chicken embryos and semen, and through respiratory and digestive tract infection, and can also be transmitted through airborne media. 1
GB19442—2004
4.2 Diagnostic methods
See GB/T 18936.
4.3 Clinical diagnostic indicators
4.3.1 Acute onset and death, sometimes no obvious lesions are found in acute death cases. 4.3.2 Bleeding of foot scales.
4.3.3 Bleeding of combs or edema of hair, head and face in chickens. 4.3.4 Waterfowl such as ducks and geese may show neurological and diarrheal symptoms, and sometimes corneal inflammation or even blindness. 4.4 Pathological diagnostic indicators
4.4.1 Extensive and severe bleeding in muscles and other tissues and organs. 4.4.2 Extensive congestion and bleeding of the mucosa of the digestive tract and respiratory tract; increased mucus in the glandular stomach, bleeding of the glandular stomach papilla, and band-like bleeding in the mucosa at the junction between the glandular stomach and the gizzard.
4.4.3 Milky white secretions or clots may be seen in the middle of the fallopian tube; the follicles may be congested, hemorrhaged, atrophied, and ruptured, and some may show "yolk peritonitis". 4.4.4 Necrotic foci, perivascular lymphocyte cuffs, glial foci, vascular hyperplasia and other lesions may appear in the brain, and focal necrosis of pancreatic and myocardial tissues may occur.
4.5 Serological diagnostic indicators
4.5.1 The hemagglutination inhibition (H1) titer of H5 or H7 reaches 24 or above. 4.5.2 Avian influenza agar immunodiffusion test (AGID) is positive (except waterfowl). 4.5.3 Avian influenza enzyme-linked immunosorbent assay (EI.ISA) is positive. 4.6 Etiological diagnostic indicators
4.6.1 H5 or H7 subtype virus isolation is positive, the virus can grow on sensitive cells lacking trypsin and produce obvious cytopathic effect (CPF), and the amino acid sequence determination results of the hemagglutinin gene cleavage site are consistent with the gene sequence of the highly pathogenic avian influenza isolate. 4.6.2 Specific highly pathogenic avian influenza molecular biological method test diagnosis is positive. 4.6.3 The intravenous inoculation pathogenicity index (IVPI) is greater than 1.2. 4.7 Result determination
4.7.1 The clinical suspicion of highly pathogenic avian influenza meets the clinical diagnostic index 4.3.1, and at least one of the clinical diagnostic indexes 4.3.2, 4.3.3, 4.3.4, or at least one of the pathological diagnostic indexes 4.4.1, 4.1.2, 4.4.3, 4.4.4. 4.7.2 The suspected highly pathogenic avian influenza
Non-immunized poultry meets the result determination 4.7.1, and meets the serological diagnostic indexes 4.5.1, 4.5.2 or/and 4.5.3; immunized poultry meets the result determination 4.7.1, and Newcastle disease and toxic diseases can be ruled out. 4.7.3 Confirmed
Conform to the result judgment 4.7.2, and at least meet one of the etiological diagnosis indicators 4.6.1, 4.6.2, 4.6.3; or conform to the result judgment 4.7.1, and meet one of 4.6.1, 4.6.2, 4.6.3. 5 Epidemic report
5.1 Any unit or individual who finds poultry suffering from this disease or suspected of this disease shall immediately report to the local animal epidemic prevention supervision agency. 5.2 After receiving the epidemic report, the local animal epidemic prevention supervision agency shall implement the relevant provisions of the "Animal Epidemic Prevention Law of the People's Republic of China" and the "National Highly Pathogenic Avian Influenza Emergency Plan" of the State Council. 6 Epidemic handling
6.1 Once a suspected epidemic occurs, after the expert group recognized by the Ministry of Agriculture or the provincial level makes a preliminary judgment on the on-site clinical diagnosis, control measures such as isolation and blockade shall be taken immediately; if the provincial laboratory uses serological methods to test positive, it can be basically confirmed, and compulsory measures such as culling shall be taken immediately. At the same time, the diseased materials should be sent to the national reference laboratory for virus isolation and typing identification in a timely manner. 2
GB 19442—2004
6.2 The epidemic situation must be confirmed by the national reference laboratory and the regional (provincial) avian influenza professional laboratory approved by the animal husbandry and veterinary administrative department of the State Council.
6.3 Delineation of epidemic sites, epidemic areas and threatened areas
6.3.1 Measures to be taken in epidemic sites
6.3.1.1 Kill all poultry, and dispose of all dead poultry, poultry killed and their poultry products harmlessly in accordance with national standards. 6.3.1.2 Dispose of poultry excrement, contaminated feed, bedding, sewage, etc. harmlessly. 6.3.1.3 Strictly and thoroughly disinfect contaminated articles, vehicles, utensils, poultry houses and sites. 6.3.2 Measures to be taken in the epidemic area
6.3.2.1 Set up warning signs around the epidemic area, set up animal quarantine and disinfection stations at traffic intersections in and out of the epidemic area, and disinfect personnel and vehicles in and out of the epidemic area.
6.3.2.2 Kill poultry in the epidemic area according to regulations. 6.3.2.3 Close live poultry and poultry product trading markets, prohibit the entry and exit of susceptible live poultry and the transportation of susceptible poultry products. 6.3.2.4 Dispose of poultry excrement, contaminated feed, bedding, sewage, etc. harmlessly in accordance with national standards. 6.3.2.5 Strictly and thoroughly disinfect contaminated articles, vehicles, utensils, poultry houses and sites. 6.3.3 Measures to be taken in threatened areas
6.3.3.1 Emergency compulsory vaccination of all susceptible poultry with vaccines approved by the state, and establish complete immunization records. 6.3.3.2 Monitor the epidemic situation of poultry and grasp the dynamics of the epidemic situation. 6.4 Blockade and lifting of blockade of epidemic areas
6.4.1 Blockade
The animal husbandry and veterinary administrative department shall report to the people's government at the same level to implement blockade of the epidemic area. After receiving the report, the people's government shall immediately make a decision and issue a blockade order.
6.4.2 Lifting of the blockade
After all poultry and their products in the epidemic area are handled in accordance with regulations, the relevant places and items shall be thoroughly disinfected under the supervision and guidance of the local animal epidemic prevention supervision agency. 21 days after the last poultry is culled, after being approved by the animal epidemic prevention supervision agency, the local animal husbandry and veterinary administrative department shall apply to the local people's government that originally issued the blockade order to issue a lifting order. After the blockade of the epidemic area is lifted, the epidemic situation in the area shall continue to be monitored. If no new cases are found after 6 months of supervision, the epidemic can be declared eradicated. 6.5 Killing
6.5.1 If it is confirmed to be highly pathogenic avian influenza, all poultry in the epidemic area shall be killed under the supervision and guidance of the animal epidemic prevention supervision agency. 6.5.2 Methods for killing live poultry:
6.5.2.1 Asphyxiation
First, put the poultry to be killed into bags, place them in a sealed car or other sealed container, and pass carbon dioxide into the bags to kill them; or put the poultry into a sealed bag and pass carbon dioxide into the bags to kill them. 6.5.2.2 Neck twisting
Used when the number of birds to be killed is small. According to the size of the poultry, hold the head with one hand and the body with the other hand, twist and stretch in opposite directions. 6.5.2.3 Others
Other killing methods that can avoid the spread of pathogens can also be used according to local conditions. 6.6 Harmless treatment
6.6.1 All dead and diseased poultry, culled poultry and their poultry products (including poultry meat, eggs, semen, feathers, down, viscera, bones, blood, etc.) shall be handled in accordance with GB16548; poultry excrement and contaminated or potentially contaminated bedding, feed and other items shall be harmlessly treated by incineration, sealed stacking and fermentation.
6.6.2 When poultry households need to transport, leak-proof containers should be used, with obvious signs, and the transportation should be carried out under the supervision of animal epidemic prevention supervision agencies. 6.7 Treatment records
The animal husbandry and veterinary administrative departments of people's governments at all levels must record the emergency treatment process of the epidemic in full and detail. 3
GB 19442--2004
7 Prevention and control
7.1 Strengthen feeding management and improve the level of environmental control. Feeding, production and business premises must meet animal epidemic prevention conditions and obtain animal epidemic prevention certificates. The breeding farm implements the all-in and all-out breeding mode, controls the personnel, vehicles and articles entering and leaving the production grid, and implements the cleaning and disinfection procedures. Chickens and waterfowl are prohibited from being mixed. The chicken farm and waterfowl breeding farm should be separated by more than 3km, and they shall not share the same water source. The poultry farm should have good facilities to prevent birds (including waterfowl) from entering the breeding area, and have sound rodent and insect control facilities and measures. 7.2 Strengthen disinfection and do a good job in basic epidemic prevention work. All breeding farms, slaughterhouses (farms), animal epidemic prevention supervision and inspection stations, etc. should establish a strict sanitation (disinfection) management system. 7.3 Monitoring
7.3.1 Organization and implementation unit
Organized and implemented by animal epidemic prevention supervision agencies at or above the county level. 7.3.2 Monitoring methods
Unimmunized areas: mainly epidemiological investigation and serological monitoring (including agar diffusion test, hemagglutination inhibition test, etc.), combined with pathogen isolation and toxin type identification, virulence identification for monitoring. Immunized areas: mainly pathogen monitoring, combined with serological monitoring. 7.3.3 Monitoring objects
Mainly chickens, turkeys, ducks, geese, etc., but also include susceptible poultry such as quail, pheasant, partridge, ostrich, pigeon, peacock and migratory birds. 7.3.4 Monitoring links and measures
7.3.4.1 Origin monitoring: All poultry farms can be monitored by pathogenic monitoring methods. All original, great-grandparent, grandparent and parent generation poultry farms, poultry farms with export tasks, and commercial generation poultry farms should be monitored twice a year; free-range poultry are randomly sampled.
Sampling ratio: Cotton swab samples from the throat or cloaca of 10 birds in each group are collected, placed in the same container, mixed into one sample, used for chicken embryo inoculation, and virus isolation.
For unimmunized poultry farms, serological monitoring is the main method. For poultry farms with export missions, sampling shall be conducted at a rate of 0.5% per batch; for poultry farms with more than 100,000 poultry per batch, sampling shall be conducted at a rate of 0.1%; for all other poultry farms, monitoring shall be conducted at least once every six months. For poultry farms with parent generation and above, monitoring shall be conducted at a rate of 0.5% per batch (group); for poultry farms with commercial generation, monitoring shall be conducted at a rate of 0.1% per batch (group). Random sampling of poultry shall be conducted at irregular intervals. The number of samples to be monitored for each batch (group) shall not be less than 20. 7.3.4.2 Monitoring of circulation links: Conduct irregular etiological and serological monitoring of live poultry and poultry products transferred from trading markets, poultry slaughter squares (fields), and other places.
7.3.5 Processing of monitoring results
The monitoring results shall be summarized in a timely manner and reported to the National Animal Husbandry and Veterinary Center regularly by the provincial animal epidemic prevention supervision agency. If etiologically positive and non-immune poultry serologically positive are found, they shall be reported and handled immediately in accordance with the relevant provisions of the Animal Epidemic Prevention Law of the People's Republic of China. During monitoring, if serologically positive poultry flocks are found due to the use of avian influenza vaccines that have not been approved by the State Council’s animal husbandry and veterinary administrative department, they shall be treated as avian influenza outbreaks.
7.3.6 Immunization
In the event of an outbreak, all susceptible poultry in the threatened area shall be urgently immunized. The vaccine used must be an avian influenza vaccine approved for use by the State Council’s animal husbandry and veterinary administrative department, and the provincial animal husbandry and veterinary stations shall organize and supply it in a unified manner.
7.3.7 Quarantine of introduced breeds
When introducing breeders, semen, and eggs from other places in China, quarantine approval procedures shall be handled at the local animal epidemic prevention supervision agency and quarantine shall be qualified. The introduced breeders must be isolated and raised for more than 21 days, and tested by the animal epidemic prevention supervision agency. Only after passing the test can they be mixed and raised. 4
When introducing breeders, semen, and eggs from abroad, the relevant national regulations shall be followed. 8 Standards for highly pathogenic avian influenza-free zones
Highly pathogenic avian influenza-free zones must meet the following conditions: 8.1 There must be regular and rapid animal epidemic reporting records. GB19442—2004
8.2 No highly pathogenic avian influenza has occurred in the past three years; no avian influenza vaccine has been administered in the past six months; no poultry vaccinated with avian influenza vaccine has been introduced after cessation of vaccination. 8.3 There must be an effective monitoring system and monitoring area in the area, and disease monitoring has been implemented in the past three years. No H5 or H7 pathogens have been detected, and the H5 or H7 avian influenza HI test is negative.
8.4 All reports, monitoring records and other relevant materials are accurate, detailed and complete. 8.5 If highly pathogenic avian influenza occurs, the zone may be re-approved for as a zone free of highly pathogenic avian influenza only after effective epidemic monitoring and serological testing have been implemented and confirmed that the requirements of 8.1, 8.2, 8.3 and 8.4 have been met, 6 months after the last sick poultry has been culled, or 6 months after the last immune poultry has been slaughtered, under the conditions of culling measures and serological monitoring and emergency immunization.1 Strengthen feeding management and improve the level of environmental control. Feeding, production and business sites must meet the conditions for animal epidemic prevention and obtain animal epidemic prevention certificates. The breeding farm implements a full-in and full-out feeding method, controls the entry and exit of personnel, vehicles and related items, and implements cleaning and disinfection procedures. Chickens and waterfowl are prohibited from being mixed. Chicken farms and waterfowl farms should be more than 3km apart and must not share the same water source. Poultry farms must have good facilities to prevent birds (including waterfowl) from entering the breeding area, and have sound rodent and insect control facilities and measures. 7.2 Strengthen disinfection and do a good job in basic epidemic prevention. All breeding farms, slaughterhouses (farms), animal epidemic prevention supervision and inspection stations, etc. must establish a strict hygiene (disinfection) management system. 7.3 Monitoring
7.3.1 Organization and implementation unit
Organized and implemented by animal epidemic prevention supervision agencies at or above the county level. 7.3.2 Monitoring methods
Non-immunized areas: mainly epidemiological surveys and serological monitoring (including agar diffusion test, hemagglutination inhibition test, etc.), combined with pathogen isolation and toxin type identification, virulence identification for monitoring. Immunized areas: mainly pathogen monitoring, combined with serological monitoring. 7.3.3 Monitoring objects
Mainly chickens, turkeys, ducks, geese, etc., but also include susceptible poultry such as quail, pheasant, partridge, ostrich, pigeon, peacock and migratory birds. 7.3.4 Monitoring links and measures
7.3.4.1 Origin monitoring: All poultry farms can be monitored by pathogen monitoring methods. All original, great-grandparent, grandparent and parent generation poultry farms, poultry farms with export tasks, and commercial generation poultry farms should be monitored twice a year; free-range poultry are randomly inspected.
Sampling ratio: Take cotton swab samples from the throat or cloaca of 10 birds in each group, put them in the same container, mix them into one sample, and use them for chicken embryo inoculation and virus isolation.
For unimmunized poultry farms, serological monitoring is the main method. For the monitoring of poultry farms with export tasks, sampling is carried out at a rate of 0.5% per batch; for poultry farms with more than 100,000 poultry per batch, sampling is carried out at a rate of 0.1%; all other poultry farms are monitored at least once every six months. For parent-generation and above breeding poultry farms, each batch (group) is monitored at a rate of 0.5%; for commercial generation poultry farms, each batch (group) is monitored at a rate of 0.1%. Random poultry is sampled irregularly. The number of samples for each batch (group) shall not be less than 20. 7.3.4.2 Monitoring of the circulation link: Conduct irregular etiological and serological monitoring of live poultry and poultry products transferred from trading markets, poultry slaughter squares (fields), and other places.
7.3.5 Handling of Monitoring Results
The monitoring results shall be timely summarized and reported to the National Animal Husbandry and Veterinary Center by the provincial animal epidemic prevention supervision agencies on a regular basis. If pathogen-positive and non-immunized poultry are found to be serologically positive, they shall be reported and handled immediately in accordance with the relevant provisions of the Animal Epidemic Prevention Law of the People's Republic of China. If serologically positive poultry flocks are found during monitoring due to the use of avian influenza vaccines that have not been approved by the State Council's animal husbandry and veterinary administrative department, they shall be handled as avian influenza epidemics.
7.3.6 Immunization
When an epidemic occurs, all susceptible poultry in the threatened area shall be urgently immunized. The vaccine used must be an avian influenza vaccine approved for use by the State Council's animal husbandry and veterinary administrative department and shall be uniformly organized and supplied by the provincial animal husbandry and veterinary stations.
7.3.7 Quarantine of Introduction
When introducing breed poultry, semen, and eggs from other places in China, quarantine approval procedures shall be handled at the local animal epidemic prevention supervision agency and quarantine shall be qualified. The introduced breeding poultry must be isolated and raised for more than 21 days and tested by the animal epidemic prevention supervision agency. Only after passing the test can they be mixed and raised. 4
When introducing breeding poultry, semen and breeding eggs from abroad, the relevant national regulations shall be followed. 8 Standards for highly pathogenic avian influenza-free areas
Highly pathogenic avian influenza-free areas must meet the following conditions: 8.1 There are regular and rapid animal epidemic reporting records. GB19442—2004
8.2 There has been no highly pathogenic avian influenza in the past 3 years; no avian influenza vaccine has been administered in the past 6 months; after stopping vaccination, no poultry vaccinated with avian influenza vaccine has been introduced. 8.3 There is an effective monitoring system and monitoring area in the area. In the past 3 years, epidemic monitoring has been implemented, and no H5 and H7 pathogens have been detected, and the H5 and H7 avian influenza HI tests are negative.
8.4 All reports, monitoring records and other relevant materials are accurate, detailed and complete. 8.5 If highly pathogenic avian influenza occurs, the zone may be re-approved for as a zone free of highly pathogenic avian influenza only after effective epidemic monitoring and serological testing have been implemented and confirmed that the requirements of 8.1, 8.2, 8.3 and 8.4 have been met, 6 months after the last sick poultry has been culled, or 6 months after the last immune poultry has been slaughtered, under the conditions of culling measures and serological monitoring and emergency immunization.1 Strengthen feeding management and improve the level of environmental control. Feeding, production and business sites must meet the conditions for animal epidemic prevention and obtain animal epidemic prevention certificates. The breeding farm implements a full-in and full-out feeding method, controls the entry and exit of personnel, vehicles and related items, and implements cleaning and disinfection procedures. Chickens and waterfowl are prohibited from being mixed. Chicken farms and waterfowl farms should be more than 3km apart and must not share the same water source. Poultry farms must have good facilities to prevent birds (including waterfowl) from entering the breeding area, and have sound rodent and insect control facilities and measures. 7.2 Strengthen disinfection and do a good job in basic epidemic prevention. All breeding farms, slaughterhouses (farms), animal epidemic prevention supervision and inspection stations, etc. must establish a strict hygiene (disinfection) management system. 7.3 Monitoring
7.3.1 Organization and implementation unit
Organized and implemented by animal epidemic prevention supervision agencies at or above the county level. 7.3.2 Monitoring methods
Non-immunized areas: mainly epidemiological surveys and serological monitoring (including agar diffusion test, hemagglutination inhibition test, etc.), combined with pathogen isolation and toxin type identification, virulence identification for monitoring. Immunized areas: mainly pathogen monitoring, combined with serological monitoring. 7.3.3 Monitoring objects
Mainly chickens, turkeys, ducks, geese, etc., but also include susceptible poultry such as quail, pheasant, partridge, ostrich, pigeon, peacock and migratory birds. 7.3.4 Monitoring links and measures
7.3.4.1 Origin monitoring: All poultry farms can be monitored by pathogen monitoring methods. All original, great-grandparent, grandparent and parent generation poultry farms, poultry farms with export tasks, and commercial generation poultry farms should be monitored twice a year; free-range poultry are randomly inspected.
Sampling ratio: Take cotton swab samples from the throat or cloaca of 10 birds in each group, put them in the same container, mix them into one sample, and use them for chicken embryo inoculation and virus isolation.
For unimmunized poultry farms, serological monitoring is the main method. For the monitoring of poultry farms with export tasks, sampling is carried out at a rate of 0.5% per batch; for poultry farms with more than 100,000 poultry per batch, sampling is carried out at a rate of 0.1%; all other poultry farms are monitored at least once every six months. For parent-generation and above breeding poultry farms, each batch (group) is monitored at a rate of 0.5%; for commercial generation poultry farms, each batch (group) is monitored at a rate of 0.1%. Random poultry is sampled irregularly. The number of samples for each batch (group) shall not be less than 20. 7.3.4.2 Monitoring of the circulation link: Conduct irregular etiological and serological monitoring of live poultry and poultry products transferred from trading markets, poultry slaughter squares (fields), and other places.
7.3.5 Handling of Monitoring Results
The monitoring results shall be timely summarized and reported to the National Animal Husbandry and Veterinary Center by the provincial animal epidemic prevention supervision agencies on a regular basis. If pathogen-positive and non-immunized poultry are found to be serologically positive, they shall be reported and handled immediately in accordance with the relevant provisions of the Animal Epidemic Prevention Law of the People's Republic of China. If serologically positive poultry flocks are found during monitoring due to the use of avian influenza vaccines that have not been approved by the State Council's animal husbandry and veterinary administrative department, they shall be handled as avian influenza epidemics. bzxZ.net
7.3.6 Immunization
When an epidemic occurs, all susceptible poultry in the threatened area shall be urgently immunized. The vaccine used must be an avian influenza vaccine approved for use by the State Council's animal husbandry and veterinary administrative department and shall be uniformly organized and supplied by the provincial animal husbandry and veterinary stations.
7.3.7 Quarantine of Introduction
When introducing breed poultry, semen, and eggs from other places in China, quarantine approval procedures shall be handled at the local animal epidemic prevention supervision agency and quarantine shall be qualified. The introduced breeding poultry must be isolated and raised for more than 21 days and tested by the animal epidemic prevention supervision agency. Only after passing the test can they be mixed and raised. 4
When introducing breeding poultry, semen and breeding eggs from abroad, the relevant national regulations shall be followed. 8 Standards for highly pathogenic avian influenza-free areas
Highly pathogenic avian influenza-free areas must meet the following conditions: 8.1 There are regular and rapid animal epidemic reporting records. GB19442—2004
8.2 There has been no highly pathogenic avian influenza in the past 3 years; no avian influenza vaccine has been administered in the past 6 months; after stopping vaccination, no poultry vaccinated with avian influenza vaccine has been introduced. 8.3 There is an effective monitoring system and monitoring area in the area. In the past 3 years, epidemic monitoring has been implemented, and no H5 and H7 pathogens have been detected, and the H5 and H7 avian influenza HI tests are negative.
8.4 All reports, monitoring records and other relevant materials are accurate, detailed and complete. 8.5 If highly pathogenic avian influenza occurs, the zone may be re-approved for as a zone free of highly pathogenic avian influenza only after effective epidemic monitoring and serological testing have been implemented and confirmed that the requirements of 8.1, 8.2, 8.3 and 8.4 have been met, 6 months after the last sick poultry has been culled, or 6 months after the last immune poultry has been slaughtered, under the conditions of culling measures and serological monitoring and emergency immunization.5 If highly pathogenic avian influenza occurs, the zone may be re-approved for being a zone free of highly pathogenic avian influenza only after effective epidemic monitoring and serological testing have been implemented and confirmed, and after meeting the requirements of 8.1, 8.2, 8.3 and 8.4, 6 months after the last sick poultry has been culled, or 6 months after the last immune poultry has been slaughtered, under the conditions of culling measures, serological monitoring and emergency immunization.5 If highly pathogenic avian influenza occurs, the zone may be re-approved for being a zone free of highly pathogenic avian influenza only after effective epidemic monitoring and serological testing have been implemented and confirmed, and after meeting the requirements of 8.1, 8.2, 8.3 and 8.4, 6 months after the last sick poultry has been culled, or 6 months after the last immune poultry has been slaughtered, under the conditions of culling measures and serological monitoring and emergency immunization.
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