This standard specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling apple leaf rollers (Adoxophyes orana). This standard applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling apple leaf roller larvae for registration. Other field efficacy tests shall refer to this standard. GB/T 17980.8-2000 Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling apple leaf rollers GB/T17980.8-2000 Standard download decompression password: www.bzxz.net
This standard specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling apple leaf rollers (Adoxophyes orana). This standard applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling apple leaf roller larvae for registration. Other field efficacy tests shall refer to this standard.

GB/T17980.8-2000
Field efficacy test is one of the important contents of pesticide registration management in my country, and is an important technical basis for the formulation of pesticide product labels, while labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, we will continue to formulate national standards for my country's field efficacy test guidelines. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Leaf roller is one of the important pests of apple trees in my country, and pesticides are often used for prevention and control in production. In order to determine the optimal field use concentration for the prevention and control of apple leaf roller, test the effects of pesticides on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and rational use technology of pesticide registration, this standard is specially formulated. This standard is one of the standards in the Guidelines for Field Efficacy Trials of Pesticides (I) series, but it is an independent standard in itself. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This standard is drafted and interpreted by the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this standard are Jiang Hui, Wang Xiaojun, Chen Jingfen, Xia Yan, Hao Shixiong and Wang Shaocheng. 373
1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I)
Pesticide-
Guidelines for the field efficacy trials (I)-Insecticides against leaf minor on appleGB/T 17980. 8—-2000
This standard specifies the methods and basic requirements for field efficacy trials of insecticides against apple leaf rollers (Adoxophyes orana).
This standard applies to the field efficacy plot test and efficacy evaluation of insecticides for the control of apple leaf roller larvae. Other field efficacy tests shall be carried out in accordance with this standard.
2 Test conditions
2.1 Selection of test objects, crops and varieties The test objects are the larvae of apple leaf rollers, and the overwintering generation or first generation larvae can be selected at will. The test crops should be the main cultivated varieties. Record the variety name. 2.2 Environmental conditions
Select representative orchards for the test. The cultivation conditions (soil type, fertilizer, tillage, plant spacing) of all test plots must be uniform and consistent, and conform to local scientific agricultural practices (GAP). 3 Test design and arrangement
3.1 Agents
3.1.1 Test agents
Indicate the trade name/code, Chinese name, generic name, dosage form content and manufacturer of the agent. The treatment of the test drug shall be no less than three doses or the dose specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control drug
The control drug must be a registered product that has been proven to have good efficacy in practice. The type and mode of action of the control drug should be similar to that of the test drug and the local commonly used dose should be used. Special circumstances may depend on the purpose of the test. 3.2 Plot arrangement
3.2.1 Plot arrangement
The plots of the test drug, control drug and blank control are arranged in random blocks. Special circumstances must be explained. 3.2.2 Plot area and repetition
Plot area: 3 to 5 trees.
Approved by the State Administration of Quality and Technical Supervision on February 1, 200071
Implemented on May 1, 2000
Number of repetitions: at least 4 repetitions.
3.3 Application methodbzxz.net
3.3.1 Application method
GB/T 17980.8--2000
Perform according to the requirements of the agreement and the instructions on the label. Application of pesticides should be adapted to local scientific agricultural practices. 3.3.2 Equipment used
Use the equipment commonly used in production. It is advisable to use a high-capacity motorized sprayer. A backpack blower mist machine can also be used. Record all the information on the type of equipment used and the operating conditions (operating pressure, nozzle diameter). The amount of pesticides should be accurate and evenly distributed when applying pesticides. The deviation of the amount of pesticides exceeding ±10% should be recorded.
3.3.3 Application time and frequency
Perform according to the requirements of the agreement and the instructions on the label. Generally, the pesticides are applied twice, with an interval of 10 to 15 days. If the test is carried out during the hibernation period of the overwintering generation larvae, the pesticides should be applied once before and after flowering. Record the amount and date of each application. 3.3.4 Dosage and volume
Apply the pesticide according to the agreement and the concentration indicated on the label. Usually the active ingredient content in the pesticide is expressed as mg/kg or mg/L (milligrams per kilogram or milligrams per liter). When used for spraying, the multiple of the drug and the amount of the liquid per hectare (L/hm (liters per hectare)) should be recorded at the same time. 3.3.5 Requirements for pesticide data for the prevention and control of other pests and diseases If other pesticides are used, pesticides that have no effect on the test pesticides and test objects should be selected, and all plots should be treated uniformly. They should be used separately from the test pesticides and control pesticides to minimize the interference of these pesticides. Record the accurate data of the application of such pesticides.
4 Survey, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type, daily rainfall in mm) and temperature (daily average temperature, maximum and minimum temperature in °C) data should be obtained from the test site or the nearest meteorological station. The adverse weather factors that affect the test results during the entire test period, such as severe or long-term dry cover, rainstorms, ice buds, etc., must be recorded. 4.1.2 Soil data
Record the data on soil type, topography, soil fertility, irrigation conditions and soil cover such as weeds. 4.2 Survey methods, time and frequency
4.2.1 Survey methods
Survey two trees in each plot and record the number of insect bags on 100 to 200 branches in the upper crown. When conducting the pre-drug base survey, peel and check the insect rate of 100 insect bags in the entire test area to correct the insect bag base. For the investigation after the application of pesticides, the rate of insects (larvae, pupae and pupa shells) in peeling 30 to 50 insect bags in the corresponding time and plot is used to correct the corresponding number of insect bags. The number of damaged branches must also be recorded in the last investigation. 4.2.2 Investigation time and number of times
The base number of investigations before the application of pesticides is 3 days, 10 days after the first application of pesticides and 15 days after the second application of pesticides, for a total of 4 investigations. 4.2.3 Calculation method of drug efficacy
The drug efficacy is calculated according to formula (1) and formula (2):
Branch damage rate (%) = number of investigated branch points + number of branches corrected × 100 Total number of investigated branches
CKPT × 100
Control effect (%) = 9
In the formula; CK--the number of insects in the blank control area;
PT.-the number of insects in the pesticide treatment area.
4.3 Direct effects on crops
(1)
GB/T17980.8—2000
Observe whether the pesticide has any damage to the crops and record the type and extent of the damage. In addition, the beneficial effects on the crops (such as accelerated maturity, increased vitality, etc.) should also be recorded.
Record the damage in the following ways:
a) If the damage can be counted or measured, it should be expressed in absolute values, such as plant height. b) In other cases, the extent and frequency of the damage can be estimated by the following two methods: 1) Record the damage in each plot according to the damage grading method, expressed as one, ten, ten ten, ten ten ten, ten ten ten ten. Grading method for pesticide damage:
·No pesticide damage;
+10: Mild pesticide damage, does not affect the normal growth of crops; +10+10: Moderate pesticide damage, can be recovered, will not cause crop yield reduction; +10+10: Severe pesticide damage, affects the normal growth of crops, causes a certain degree of loss in crop yield and quality; +10+10: Severe pesticide damage, crop growth is hindered, and crop yield and quality loss are serious. 2) Compare the pesticide-treated area with the blank control area to evaluate the percentage of pesticide damage. At the same time, accurately describe the pesticide damage symptoms of crops (dwarfing, chlorosis, deformity, etc.). 4.4 Impact on other organisms
4.4.1 Impact on other pests and diseases
Any impact on other pests and diseases should be recorded, including beneficial and unbeneficial impacts. 4.4.2 Impact on other non-target organisms
Record any impact of the pesticide on wildlife and beneficial insects. 4.5. Product quality and yield. Generally, no record is required.
5. Results. Use the Duncan's New Multiple Range (DMRT) method to analyze the test data. In special cases, use the corresponding biostatistical methods. Write a formal test report and analyze and evaluate the test results. The test report should list the original data. 876
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