This standard applies to the seed germination test of commercial grains and oilseeds. GB/T 5520-1985 Seed germination test for grain and oilseed inspection GB/T5520-1985 Standard download decompression password: www.bzxz.net

National Standard of the People's Republic of China
Inspection of grain and oilseedsGermination test of seeds
This standard applies to the germination test of commercial grain and oilseeds seeds. 1 Apparatus and tools
1.1 Petri dish:wwW.bzxz.Net
1.2 Filter paper,
1.3 Germination box or electric thermostat,
1.4 Tweezers.
2 Technical regulations for germination test
Technical regulations for germination test are shown in Table 1:
Seed name
Germination bed
Filter paper or sand
Filter paper or sand
Filter paper or sand
Temperature bar
Note: Rice seeds are first planted with 30℃ water for 24 hours, and cotton seeds are first planted with 60℃ water for 3-4 hours. 3
Preparation of germination bed
UDC (633.1+ 633.85)
.001.4
GB 5520--85
Germination potential days
Germination rate days
According to the technical regulations, choose an appropriate germination bed, lay 1cm thick washed fine sand or two layers of filter paper in the culture dish or plate, and inject clean water until it reaches saturation.
All utensils and germination beds used in germination tests must be sterilized by steam or boiling water. Issued by the National Bureau of Standards on November 2, 1985
Implemented on July 1, 1986
4 Test Method
GB5520-85
4.1 Sample Preparation: Randomly select 4 groups of samples from the good seeds that have been tested for purity: large seeds such as peanuts, soybeans, corn and peas, etc., with 50 seeds in a group (50×4) and medium and small seeds such as rice, wheat, millet, rapeseed, etc., with 100 seeds in a group (100×4). 4.2 Seed Placement: Place the seeds in groups on the germination bed, with the distance between the seeds being 1~~2 times the length of the grain. After placing (the seeds and fine sand can be flattened for the sand bed), cover them, but do not hinder air circulation. 4.3 Mark and send them to the germination box: Attach a label to the germination dish, indicating the sample number, variety name, and test start date. Or just number the germination bed and set up a separate germination test record. Finally, the germination bed is sent to the germination box or constant temperature box, and the germination test is carried out according to the temperature and days specified in the technology.
4.4 Inspection: After the germination test begins, in addition to maintaining the moisture and temperature required for germination, the germination situation is checked once a day. According to the deadline for germination potential and germination rate, normal and abnormal germination seeds are checked in time and records are made. 4.4.1 Normal germination seeds
4.4.1.1 The young roots of long-grain cereal seeds are as long as the seed, and the young sprouts are at least half of the length of the grain. 4.4.1.2 The young roots and young sprouts of monocotyledonous round seeds are as long as the diameter of the seed, and the young roots of dicotyledonous round seeds are as long as the diameter of the seed. 4.4.1.3 Legume seeds have normal young roots, and at least one cotyledon is connected to the young root or more than two-thirds of the two cotyledons are retained. 4.4.1.4 Although cereal seeds have no taproot, their lateral roots develop normally. 4.4.2 Abnormally germinated seeds
4.4.2.1 Seeds of cereals with incomplete, deformed or rotten young roots or young sprouts. 4.4.2.2 Young roots are significantly shrunken, with fibrous young roots in the middle, and young sprouts edematous and without root hairs. 4.4.2.3 Both cotyledons of legume seeds are completely detached or more than one-third are damaged. Hard grains in legumes are generally counted as one-half of the germinated seeds.
4.4.3 Determine the degree of aging of grain quality When identifying germination, the fresh young sprouts that break through the seed coat (commonly known as whitening) are considered normal germinated grains.
5 Calculation of results
Seed germination tests are expressed in terms of germination potential and germination rate. Seed germination potential and seed germination rate are calculated according to formula (1) and formula (2) respectively:
Seed germination potential (%)
Seed germination rate (%)
Wherein: M—Number of normal germinated grains within the germination potential days, M2—Number of all normal germinated grains,
M#x100
M-Number of test seeds.
Seed germination potential and germination rate are expressed as the average value of four groups of test results. Whether the average value is reliable depends on whether the difference between the maximum and minimum values ​​of the four parallel determinations exceeds the allowable difference range listed in Table 2. The allowable difference between the maximum and minimum values ​​of the germination rate and the average value of the germination rate are shown in Table 2: 282
Average value of germination rate
Average value of germination rate
11～12
15～17
GB5520--85
Allowable difference between the maximum and minimum values
Average value of germination rate
Average value of germination rate
Allowable difference between the maximum and minimum values
If the difference between the maximum and minimum values ​​of the four parallel results is less than the given range in the table, it means that the average value is reliable and can be used as the germination rate of the sample. If it exceeds the given range, the average value is unreliable and a second test should be conducted. The average value of the second test is also checked for reliability using the same method. If it is reliable, the second result is used as the sample germination rate. If the second average value is still unreliable. The average values ​​of the first and second tests can be compared. If the difference does not exceed the value given in Table 3, the average value can be used as the germination rate of the sample. Table 3
Germination rate
Average value
91～94
11～16
"Germination rate
42～50
If it exceeds the given value, a third test should be conducted and the result that meets the given value in Table 2 or Table 3 should be taken as the germination rate of the sample. Additional remarks;
This standard was proposed by the Ministry of Commerce of the People's Republic of China. This standard was drafted by the Grain Storage and Transportation Bureau of the Ministry of Commerce. The main drafters of this standard are Gao Xiuwu, Yang Haoran, Wu Yanxia and Lv Guifen. 283
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