Some standard content:
rCsa7.080
Agricultural Industry Standard of the People's Republic of China
NY/T 629—2002
2002-12-30 Issued
2003-03-01 Implementation
Issued by the Ministry of Agriculture of the People's Republic of China
The insulation resistance of this standard is the standard resistance.
This standard is issued by the Ministry of Agriculture of the People's Republic of China. Former
This standard is revised by: Institute of Honeycomb, China Agricultural University. The subsequent unit of this standard is: Beijing Shibang Zhencai Technology Development Co., Ltd. The main contributors of this standard are: Xu Pin, Lu Shangyi Lai Shu Xie Shiyu NY/T629—2002
1 Scope
This standard specifies the definition of rubber, quality requirements, test methods, inspection specifications, labeling, packaging and transportation requirements. This standard applies to the production, processing and marketing of rubber. 2 Normative references
NY/I6292002
The following documents have become clauses of this standard through reference in this standard. All the applicable documents that can be used in due course shall be used in accordance with all revisions (excluding the contents of errors) or versions that you subscribe to. However, the parties to this standard shall discuss whether the updated versions of these documents can be used. For all the referenced documents that are not in due course, the updated versions shall apply to this standard. GB/T191 Packaging end indication Marking
Head H/T01 Chemical agent content analysis (narrow analysis) mark for difficult to fall the cutting of the trade GB1631 Food packaging material nylon resin hygiene standard G1E2 Food packaging material nylon molding hygiene standard GB/T5009.12 Food lead in food side 152C1 Food container, packaging material ethylene oxide vinyl chloride co-product health cattle mark 3 terms and definitions
The following terms and definitions apply to wood labels.
Rubber propolis|| tt||The sticky substance formed after bees combine the secretions of pollen from leaves and callus with a small amount of pollen from wax glands, etc.,
Total ictalflavoids
The total content of ictalflavoids and ictal-like substances in propolis. This label is based on the high content of queruetin, myicetin, kaemptervl, and chrysanthemum in propolis. apigenin), pine (pinocembr:n), chryaine (chrysanthemum) and other flavonoids (3.3
Gxidation time
Chemical time
The comprehensive normalized energy of the bee cavity), usually refers to the time required for the red color of a certain area of the bee cavity to completely disappear when the concentration of C.0 diamacid egg has a certain rest plan, and the quality requirement is measured by sand ($).
4. 1 Sensory index of bee bites! Regulations. Nx/T629-—2002
Superior products
Sensory indicators
School products
Opaque non-use blocks or pieces, below 1°C for the same body, ℃ also broken, sticky and plastic, above 0°C for the same body
There are strong longevity swallowing skin
Package, etc., with beautiful light
I taste Xin Ping love investment 42 Physical and chemical indicators: have a light and strong taste, good color, perfect luster, and the physical and chemical indicators of enamine should meet the requirements of Table 2, with obvious fragrance, gray-black color, light and obvious bitterness. Table 2 Physical and chemical indicators of wax and beer. See 5) Oxidation time/total dosage/1% dosage: in h>If the mark, packaging and content are unqualified, the production unit is allowed to apply for re-testing once after rectification: If the inspection results do not meet the indicators specified in this standard, re-testing is allowed once. The re-testing should be carried out on samples from the same batch of products, and the results shall prevail. If the indicators are unqualified, re-testing is not allowed. 7 Marks
7.1 Food labels
shall comply with the requirements of GH/T18, and the marks on the transport packaging shall be consistent with the labels of qualified products: 7.2 Graphic marks
shall comply with the requirements of GH/T191.
8 Packaging, transportation and storage
8.1 Packaging
8.1.1 Packaging materials shall comply with the requirements of food safety standards. B, 1,? The packaging should be solid, flexible and easy to load and unload, store and transport. 8.1.3 Products of the same origin and specifications shall be packaged separately. 8.2 Transportation
8.2.1 During transportation, the transportation tools shall be clean, odorless and non-polluting. Products shall not be mixed with noisy, harmful, smelly and easily polluted items.
8.2.2 During transportation, it is strictly prevented from being wetted by the sun. Loading and unloading shall be done gently. 3
NY/T 6292002
8.3.1 When inspected or stored for a short period of time, it should be stored in a cool, dry and hygienic place. It should be strictly away from the sun, the sun and pollution of toxic and organic substances. B.3.2 When stored for a short period of time, it should be separated according to product grade and specifications. The storage place should be kept cool and dry. 8.3.3 The product should not be stored together with toxic, harmful and foreign objects: A.1 Determination of total propolis content
A.1.1 Principle
(Normative Appendix]
Testing methods for physical and chemical indicators of propolis
NY/T 629—2002
On the mother nucleus, oxygen is released, and a series of flavonoids or flavonoids are formed. The following are the ones collected by bees: galangal, galangin, pine, and pine. They all absorb at ultraviolet wavelength 2. These 8 flavonoids have different effects on the internal structure and do not produce hydroxylation. They have different retention times on the column. As the number of bases decreases, the retention time is called H. Therefore, the order of the following eight flavonoids peaks should be: galangal → galangal → pine + apigenin → galangin → galangin. The flavonoid content in these gums was determined by reverse HPLC, which was fast, accurate, and precise.
A. 1.2 Reagents: 1) 1-hydroxy-2-nitropropene, 1-hydroxy-2-nitropropene, 1-hydroxy-2-nitropropene, 1-hydroxy-2-nitropropene, 1-hydroxy-2-nitropropene, 1-hydroxy-2-nitropropene (chromatographically pure), 1-hydroxy-2-nitropropene (superior purity, filtered through 0.5 μm filter), 1-hydroxy-2-nitropropene (superior purity), 1-hydroxy-2-nitropropene (heavy distilled water, filtered through 0.4 μm buffer). 4.1.3 Equipment and instruments: Ultrasonic equipment: 1) bipolar transducer; ||High pressure phase chromatography (external detector):
A.1.4 color matching system
Color mixing: KramasilC;(o.4X2c)t
Infusion phase: ethanol-35% water (use [H to 3) flow rate: U.7F1./m1
Detector: t270nmX.1AUFS
Setting formula: external standard method)||t A.1.5 Step A.1.5.1 Preparation of standard solution Accurately weigh 100 mg of standard sample (200 mg + 500 ml) and put it into a container with 0.1 ml of sample solution. A.1.5.2 Preparation of sample solution Weigh 0.1 g of sample (100 mg + 500 ml) and put it into a container with 0.1 ml of sample solution. ,C people ml. Methanol is heated in supernatant water to oscillation min. After being dissolved, it is filtered with 0.5% sodium hydroxide and the filtrate is used as a separator. A.1.5.3 Determination
Under the above color conditions, take the standard wave for analysis and get the negative spectrum: repeat the sample 6 times and calculate the average value of the evaluation area; under the same color conditions, take 101. The sample was analyzed and the content of each flavonoid in the sample was calculated by external standard method: 4, 1.5. 4 Calculation
See branch. 1)
MXA: XV X_(10
NYT629—2002
Wu Zhong:
The content of various flavonoids in every 100 samples, in grams (tug); M——The content of each flavonoid in 10 standard samples, in grams); A-The average value of the peak surface of various corresponding flavonoids in the sample wave of one UL. The volume of a sample, in microliters (pl); W——The weighed amount of the sample, in grams (g) A: The peak and half-mean of the yellow energy in 1L standard solution: The sample output unit is microliter: 1.2 Determination of propolis oxidation time
A.2.1 Determination principle
Potassium manganate is a strong oxidizing agent. Its oxidation performance is the best under acidic conditions. Mn () ions are red in concentrated form. After sensitive reduction, 5 colors of Mn-2+ are obtained. The color of the solution is as follows: Mn0, ++h +ir---+Mn! +dh,O
colorless)
The chemical composition of this stock is extremely complex, and more than 300 substances are found in the electrochemical separation, among which there are more than 100 unsaturated alcohols, aldehydes, acids, yellow alkene, and other chemical substances with reducing properties. These purple compounds react with potassium permanganate to produce an oxidation-reduction reaction, and the color of the solution changes from purple to colorless. Due to this characteristic, the time required for the complete disappearance of the purple-red potassium permanganate solution of the same quality of propolis with a fixed concentration and volume (in autumn, 9) is called the oxidation time of the sample. The short oxidation time has a strong anti-chemical ability, and the comparison of the chemical time of propolis can make corresponding decisions on their quality. www.bzxz.net
A.2.2 Reagents
3) Alcohol (S5%) analytical system
b) High-purity acid. Prepare 0.01n01/L potassium permanganate solution, that is, accurately weigh 3.1U potassium permanganate, sieve with water at a rate of 11.
0.5%~5:1% pure sulfuric acid, and prepare 20% sulfuric acid. d) Distilled water,
A.2.3 Equipment and apparatus
a) Balance (with a 100 μg display).
1) Electric pulverizer,
c) Vibrating belt.
d Seconds
25CmL solid [1 conical flask with a convex surface:
50 ml.,100 nT.,1 rr rr. Volumetric flask if T, mT conical extraction.
h) 0.2ml1.5mL.2.0ml,5.0mL10.3L process liquid +.) Stir to the maximum recommended dryness
A, 2. 4 Steps
) At room temperature, weigh (confirm) fresh sample, put it into 250ml of cone-shaped cylinder, add 25mT.z alcohol, put it in a bottle, place it on a low temperature for 1h, then add 0L distilled water, after fully mixing, filter and collect the liquid.
Transfer and absorb 0.5l. 1 reverse liquid, put it into 0m of the discarded plate with hot water and until the pressure is less than 1 rml. Weigh the concentrate, put it into 50 1ml. of standard bottle, add 3,T.2% of slurry. Vibrate 6
NY/T629—2002
1 min, then, with 0.2 A pipette with a volume of 0.05 mL of 0.01 mol/L potassium permanganate was added. At the same time as the potassium permanganate solution was added, the stopwatch was started. When the purple-red color completely disappeared, the stopwatch was stopped and the time taken for the purple-red color to completely disappear was recorded (in seconds). This was the antioxidant time of the sample. Each sample was measured twice in parallel, and the mean of the two values was taken as the measured value of the sample. A.3 Determination of the ethanol-soluble content in propolis 4.3.1 Principle of determination The main biocompatible and pharmacological components contained in the propolis raw material can be dissolved in 5% ethanol, which is used as a measure to evaluate the quality of the propolis raw material. During the determination, the mass of the ethanol part in the test extract was weighed - the mass of the 75% ethanol fraction was obtained by the reduction method, that is, the mass of the 75% ethanol extract, and the daily content in the sample was calculated. 1. 3.2 Test Method: Ethanol (95%): Hot water; c) Quantitative tissue paper Instruments and equipment Balance (or 0.001g): Electric crusher; Blast drying oven, Automatic sterilizer 1 Seat-mouthed bottle (250ml.) Recording bottle (1Wm5mt) Burn 100ml.5c0mL];
wave effect Xie Dou (1.
Method and steps
Prepare 5-way ethanol stock:
Weigh 10 accurately to 0001g of 75% 70% 7-stop commercial mortar, grind it into a shape, put the product into a cone, rinse the grinder with a small amount of ethanol solution for three times, wash and concentrate it in the male shape, make the ethanol concentration reach [1nl. Good bottle, put this bottle in a 1:, room temperature under humidification, shake every 1h for 3)in, do not mention (m! dry for 24 h;
Take a piece of previously weighed filter paper and place it in a slide bucket, filter the above extract into another conical bottle, and then rinse the original conical bottle and filter paper with a little ethanol. After the filter paper is dried at room temperature for ten minutes, it is placed in a blast oven, and then replaced with a constant temperature for ten minutes. Weigh and count the quality of the ethanol extraction: perform three parallel tests under the same conditions. A,3. 5 Calculate
Formula (A2).
X, =WW>×100
The content of ethanol extract in the sample is 1.5%;
The same quality is expressed in grams (g);
The content of the substance is expressed in grams
The error allowed for parallel tests shall not exceed 1.5%. Take the average value of three measurements, A,4, the content of beeswax and insoluble matter, and use formula (4, 3) to obtain X, and then use the reduction method to obtain the content of beeswax and insoluble matter. The specific calculation formula is shown in 4, 3): (A.3)
NY/T629—2002
In the formula,
-the content of beeswax substances is
X[——the total amount of ethanol extract,%.
= 100%-K,
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