NY/T 1443.1-2007 Technical specification for evaluation of wheat resistance to diseases and insect pests Part 1: Technical specification for evaluation of wheat resistance to stripe rust NY/T1443.1-2007 Standard download decompression password: www.bzxz.net

ICS65.020.01
Agricultural Industry Standard of the People's Republic of China
NY/T 1443.1---2007
Technical Specifications for Evaluation of Wheat Resistance to Diseases and Insect Pests
Part 1: Technical Specifications for Evaluation of Wheat Resistance to Yellw Rust(Puccinia striiformis West. f. sp.trifici Eriks.et Henn,)Published on September 14, 2007
Implementation on December 1, 2007
Published by the Ministry of Agriculture of the People's Republic of China
NY/T1443—2007≤Technical Specifications for Evaluation of Wheat Resistance to Diseases and Insect Pests" is a series of standards. Part 1: Technical specification for evaluation of wheat resistance to stripe rust; - Part 2: Technical specification for evaluation of wheat resistance to leaf rust; Part 3: Technical specification for evaluation of wheat resistance to stem rust; - Part 4: Technical specification for evaluation of wheat resistance to water blight; - Part 5: Technical specification for evaluation of wheat resistance to stripe disease; - Part 6: Technical specification for evaluation of wheat resistance to yellow dwarf disease; - Part 7: Technical specification for evaluation of wheat resistance to aphids; Part 8: Technical specification for evaluation of wheat resistance to midges: This part is Part 1 of VY/T1443.
Appendix A of this part is an informative appendix, and Appendix B is a normative appendix. This part is proposed and managed by the Ministry of Agriculture of the People's Republic of China. Drafting unit of this part: Institute of Plant Protection, Chinese Academy of Agricultural Sciences. Main drafters of this part: Chen Wanquan, Liu Taiguo, Chen Fzun, Xu Shichang. NY/T1443.1—2007
Technical Specifications for Evaluation of Wheat Disease and Pest Resistance
Part 1: Technical Specifications for Evaluation of Wheat Stripe Rust Resistance This standard specifies the technology for evaluating wheat stripe rust resistance and the method for evaluating resistance. NY/T 1443.12007
This standard is applicable to ordinary wheat (including selected varieties/lines, local varieties, special genetic materials, near-isogenic lines, recombinant inbred lines, DII populations), hybrid wheat, genetically modified wheat, other cultivated wheat species, wild wheat, wheat determination and evaluation.
2 Terms and Definitions
The following terms and definitions apply to this standard. 2.1
Field evaluation of resistance of closely related species to wheat stripe rustDisease resistance
A heritable trait of a plant that can reduce or overcome the pathogenic effects of a pathogen.2.2
Slow rust
Under environmental conditions suitable for the occurrence of the disease, the interaction between the host plant and the pathogen shows a susceptible response of type 3…4, but the disease develops relatively slowly, with the final disease index below 25.2.3
Pathogenicity
The ability of a pathogen to cause disease.2.4
Artificial inocilation2.5
Under suitable conditions, the process of placing the inoculum on the susceptible part of the plant and causing it to become diseased through artificial manipulation. Disease level diseasescale
Numerical description of the disease degree of individual or group plants Resistance evaluation: qualitative description of the host plant's reaction degree and resistance level to specific pests and diseases according to the adopted technical standards. 2.7
Isolate isoale
Pathogen inoculum obtained from the diseased part of the plant by artificial methods and cultured under specific environmental conditions
Pathogen that can infect the host plant and cause disease. Physiologic race NY/T 1443.1—2007
A taxonomic unit within a pathogenic fungus species, variant or specialized type. There is no morphological difference between the physiologic races, but there are differences in pathogenicity to differential varieties with different disease resistance genes.
Differential host
Used to identify and differentiate a set of host varieties with different resistance genes for physiological races or strains of specific pathogens. 2.11
Incidence
Or the incidence rate, the percentage of diseased plant units to the total number of investigated plant units, is used to indicate the prevalence of the disease. In this standard, the plant unit is the leaf.
Severity
The percentage of the diseased area or volume on the diseased plant unit to the total area or volume of the unit. It can also be expressed by grading, that is, the severity of the disease is divided into several levels from light to heavy, and represented by some representative values ​​to indicate the severity of the disease. 2.13
Disease index
is a comprehensive index that takes both the incidence and severity into account. When the severity is represented by the graded representative value, the disease index calculation formula is: 2(X · S)/2(X, . St) × 100
Wherein, DI is the disease index, X is the disease level (0-), X is the unit number of the level, S is the representative value of the level severity, Sx is the highest level value of the severity, and is the cumulative symbol, starting from level U (. disease-free unit). When half severity is expressed as a percentage, the following formula is used for calculation: DI = I × $ × 100
Where 1 is the prevalence rate and Li is the average severity. 2.14
Infection type infectiontype
The lesion type is divided according to the presence or absence of wheat allergic necrosis reaction and its intensity. It is used to indicate the degree of resistance of wheat varieties to stripe rust. There are six types: 0, 0; 1, 2, 3, 4. Each type can be accompanied by a "\" or "-" sign to indicate that it is heavier or lighter. 2.15
Latent period latent period
The period from when the pathogen infects wheat to establish a parasitic relationship to when the wheat is planted and symptoms show is called the latent period. 2.16
wheat yellew rust ur siripe rustFhwheat stripe rust fungus Pucxiniu strformisWest: ", ap.tniticiEriks.etTenn, is a wheat disease with rust-like lesions on the leaves. Wheat stripe disease mainly harms wheat leaves, but can also harm leaf sheaths, purple bars and spikes. When wheat is infected, chlorotic spots grow on the surface of the leaves, followed by yellow powdery sores, which are the summer spores of the pathogen, and black blisters, which are the winter spores of the pathogen. The summer spores are bright yellow, narrow and long to oblong, arranged in strips parallel to the leaf veins in the adult stage, but not in the seedling stage. Arranged in rows, forming a multi-sub-wheel shape with the infection point as the center. The winter rust pile is narrow and long, buried under the epidermis, and in a stripe shape. 3 Preparation of pathogen inoculum
3.1 Isolation of stripe rust inoculation strains
Pick a small diseased piece with typical stripe rust spots and place it on a culture leaf covered with filter paper, spread the leaves flat, and soak for 16h~24h. Use a blade to scrape the diseased spots, and apply the scraped summer spores on the diseased variety Xianxian 169, spray evenly, and place it in a moisturizing chamber at 9℃-13℃ for 2 hours. Observe the disease situation after 15d~17d and obtain the isolate. After the isolate was confirmed as Puccinzia striformis West.f.sp-tritici Friksse Henn. by morphological identification, it was purified separately, tested for pathogenicity, and then propagated and stored for future use. 3.2 Identification of physiological races of isolated stripe rust strains: The stripe rust used for resistance identification inoculation should first be identified as physiological races. The identification of physiological races uses a unified set of identification hosts (see Appendix A), and the physiological races of different strains are determined based on the disease resistance or disease susceptibility patterns (see Appendix A.1) produced by the interaction between the stripe rust and the identification host soil.
3.3 Reproduction and preservation of wheat stripe rust: "3.3.1 Seedlings
Select a flower pot with a diameter of 10 cm and a height of 10 cm, fill it with soil rich in organic matter, sow 20 to 25 healthy and plump seeds of the disease-susceptible variety Mingxian 169 in each pot, cover it with 1 cm of soil, and then place the seedlings in a seedling tray filled with water, so that the water is slowly absorbed from the bottom of the seedling pot to the surface of the soil. Remove it and cover it in a greenhouse at 15C18℃ for 6d to 8d for use. 3.3.2 Inoculation and moisturizing
Inoculation: Carry out in the operating table (or inoculation room). First, purify the air with disinfectant spray, and then use 75% ethanol to disinfect the operating table (inoculation room) and inoculation needle. Place the seedlings to be inoculated on the operating table (inoculation room). When transferring the seedlings, bag them or take other measures to prevent spore contamination in the air: write labels, record the inoculation date and the number of the inoculated strain, dip the wheat stripe rust summer spores by hand [or scrape the few summer spores from the standard sample after moisturizing), and inoculate them on the first leaf of the sterile seedlings. Mixing: Place the inoculated seedlings in the inoculation bucket, use clean water to mist, so that a uniform layer of mist droplets adheres to the surface of the leaves, seal, and moisturize for 18h~24h under the conditions of 13℃ and 13℃.
3.3.3 Strain propagation
Chrysanthemum seed propagation is divided into primary propagation and extended propagation.
Primary propagation: The summer spores of stripe rust are propagated on Mingxian 169. After the first leaf of the seedling Ming 169 is fully unfolded, take the ampoule containing the strain from the liquid nitrogen, activate it in warm water at 40℃～45℃ for 5min, then break the ampoule, place it in a culture medium containing moist filter paper, and hydrate it at 8℃-10℃ dark conditions for 10h-2ha. The initial propagation adopts the smearing method. This method is mainly used to propagate a small number of strains or inoculate a small amount of identification materials: take out a small amount of stripe rust summer spores from the ampoule or finger-shaped tube and place them on a clean frosted glass, add a small amount of water with a dropper, and use an inoculation needle to mix the stripe rust summer spores with water for later use. In addition, use a clean finger dipped in clean water or 0.05% Tween 20 (Tween20) aqueous solution to rub the wheat leaves several times to remove the wax and hair on the surface of the leaves, so as to facilitate the adsorption of the bacterial solution on the leaf surface: use the spore suspension prepared on the thawed seed needle to smear it on the front of the wheat leaves, insert a label, and record the inoculation date and the strain used. After inoculation, put the wheat into a moisturizing bucket and spray it with clean water; make the wheat seedlings and the inner wall of the wet bucket covered with droplets, and do not spray too much. After spraying, immediately cover the plastic film or glass, and place the moisturizing bucket in a dark environment of 9℃-13℃ to keep moist for 18h~24hc
When inoculating different physiological species, all the materials used for inoculation must be sterilized first to prevent possible contamination. :: Expansion: Inoculation can be carried out by smearing, sweeping, spraying (sprinkling) powder or spraying. Sweeping method: first put the Mingxian 169 Xiaoding in the inoculation bucket of the first period, spray the leaves with 0.05% Tween20 aqueous solution, then turn the diseased seedlings upside down, sweep the diseased leaves on the sterile nodes in the inoculation bucket 3 to 5 times, and then spray with 0.05% T1ween20 aqueous solution to make a uniform layer of droplets on the surface of the leaves, seal it, and place it in a dark environment of 9℃-13℃ to keep moist for 18h24h. Spray (sprinkle) powder method: After disinfection and drying, use a small powder sprayer (or a glass test tube sealed with double gauze) to evenly mix dry talcum powder and leaf rust fungus Adolphe lucidum powder in a ratio of 20 to 30:1, then add a small amount of newly collected stripe rust fungus Adolphe lucidum, mix well and set aside (the ratio of talcum powder to Adolphe lucidum is about 20-30:1). Before inoculation, wipe the leaves with water for several times to remove the wax, and evenly spray the leaves with a sprayer. Then use a duster (or a glass test tube sealed with double gauze) to evenly spray (or shake off) the diluted spore powder onto the leaves, and then spray with water. Then put it in a storage bucket, cover it with plastic film, and keep it moist in the dark at 9℃～13℃ for 18h~24ha.
Use a small sprayer to spray the newly prepared spore suspension (in a small beaker, add the stripe rust spores to be inoculated, and first use a small amount of NY/T 1443.1—2007
Moisten with water, stir into a paste, add water at a ratio of 2g spores: 1000mL, transfer to an extract sprayer; or dilute to a spore suspension at a ratio of 10mg spores: 1mL non-toxic light mineral oil-Soltral170) and spray on the wheat seedlings whose leaves have been waxed, dry naturally for 15min30rin, then put the wheat seedlings in a moisturizing bucket and keep them moist in the dark at 9℃~13℃ for 18h~24h. The spray (dust) method and the spray method are suitable for inoculation identification of a large number of materials. 3.3.4 Latent inoculation
Take the inoculated seedlings and place them in a temperature-controlled greenhouse for cultivation. Keep the day temperature at 15℃～19℃, and the night temperature at 10℃14℃; the light intensity is 600Lux~·10000Lux, and the light time is 16h. When the leaves are visible (7d after grafting), cut off the core and add a glass cover for isolation. About 15d～17d after inoculation, the summer spores will mature and be ready for use. 3.3.5 Collection of strains
The operation of air treatment and instrument disinfection is the same as 3.3.2. Take the wheat seedlings carefully and shake their leaves gently with an inoculation needle to disperse the strains into the glass cover. Remove the wheat seedlings, cover the glass cover lightly, collect the strains, and put them into a finger-shaped tube. 3.3.6 Preservation of strains
According to the length of standby time, strain preservation can be divided into short-term preservation and long-term preservation. 3.3.6.1 Short-term preservation
When the strain is to be used within 5 days to 6 days, place the finger-shaped tube containing the strain into a desiccator containing color-changing silica gel and place it in a refrigerator at 0℃~5 for standby use.
3.3.6.2 Long-term storage
When the strain is to be used in the second year or longer, long-term storage can be used. First, place the strain into a desiccator containing color-changing silica gel and place it in a refrigerator at 0℃5 for 5 days to dry, then transfer it to an ampoule tube, evacuate it, and cover it with liquid nitrogen. Or a refrigerator at 80℃, 4 Field resistance identification
Field resistance identification is carried out in the identification field: 4.1 Identification field site selection
The identification field is set in the wheat stripe rust suitable area, and the selected plots have good natural disease environment and controllable irrigation conditions, flat terrain and fertile soil.
4.2 Selection and adjustment of susceptible control varieties and induced varieties The susceptible control varieties and induced varieties used are all 9 of Ming Arm 16. 4.3 Identification field configuration and field sowing
4.3.1 Field configuration
The identification plot adopts open strip sowing and equal row spacing. The width of the row is 50CIL, the width of the row is 250cm, and the length depends on the terrain and topography; 125cm away from the row, a row of induction lines is planted in sequence, and the identification materials are planted horizontally 20cm on both sides of the induction line. The row length is 100cr and the row spacing is 33cm. Repeat 1 to 3 times, arrange in sequence, number, and set a 100cm wide protection area around the identification line. 1
1111111111111
Description:
Short-shaped frame indicates silicon!
Solid line indicates Erto release and control varieties:
·Dirty velvet indicates identification material.
Figure 1 Schematic diagram of identification solid field configuration
4.3.2 Sowing
4.3.2.1 Sowing time
NY/T 1443:1--2007
The sowing time should be consistent with the daytime production, or the sowing time of different materials should be appropriately adjusted so that the plant inoculation period and the disease period can meet the appropriate climatic conditions (condensation and temperature). Winter materials should be sown in autumn according to the local climate, weak winter materials should be sown in late autumn, and spring materials should be sown in spring. 4.3.2.2 Sowing method and sowing amount
Manual furrowing and strip sowing are adopted. One row is sown for each batch of materials, and one row of the disease-susceptible control variety Mingxian 169 is sown every 20 copies of identification materials. 100 seeds are evenly sown in each row of identification materials; 100 seeds are evenly sown in each 100 cm row length for induced rows. 4.4 Inoculation
4.4.1 Inoculation period
The inoculation period for wheat stripe rust resistance adjustment is the early stage of wheat jointing, and it should be carried out in the evening when it is sunny and windless. 4.4.2 Inoculation method
Use summer spore suspension spraying method, spraying (sprinkling) powder method or injection method to inoculate the induced line, and use the summer spore derivatives produced after the induced line to spread and infect the fixed materials: select a single species or a mixed species according to the identification needs. The specific operation is to adjust the spore powder of the selected Niu Li species with a few drops of 0.05% Twen20 aqueous solution to a state, and release it into summer spore suspension according to the ratio of 2 spores: 1000mL. Water. Set a 100cm long inoculation section every 500cm of the induced line, and use a handheld sprayer to evenly spray the summer spore suspension on the wheat leaves of the inoculation section, then quickly cover the plastic film, press it tightly with soil around, and tear off the film the next morning. Injection method, this method is mostly used in the jointing stage when the wheat seedlings have a certain height. Before inoculation, a spore suspension should be prepared (preparation method: spray method). Generally, 10 to 20 single stems are selected every 100 cm on the induction line for injection and inoculation. The method is to use a syringe to inject the spore suspension from about 1 cm below the junction of the wheat heart leaf and the leaf sheath below it. The needle should be tilted downward to pierce, but do not pierce through. Squeeze a small amount of suspension to see water droplets on the heart leaf. The suspension must be stirred or sprinkled after use. The inoculation method is best carried out in the evening on a cloudy day: if it is early in the morning, water the inoculation point 1 to 2 times, or irrigate appropriately before or after inoculation.
When inoculating again, the interval is 3α~5d, and different inoculation sections of the induction line are selected for inoculation. 4.5 Field management before and after inoculation
Field irrigation should be carried out 6-7 days before inoculation, or inoculation should be carried out after rain; if it rains continuously after inoculation, intercropping should be carried out in time, and no fungicide should be applied.
4.6 Disease investigation and recording standards
4.6.1 Investigation time
During the investigation, the disease investigation should be carried out during the wheat milk maturity period.
4.6.2 Investigation methods and items
During the investigation, 50 leaves (mainly flag leaves) were randomly sampled from each material, and the severity and infection type were investigated and recorded leaf by leaf, and the prevalence, average severity and disease index were calculated. The meal was adjusted twice with an interval of 10 days. 4.6.3 Recording and standards of disease severity
4.6.3.1 Recording and standards of infection type
The infection type of lesions is divided into six types: 0.0;, 1, 2, 3, and 4. The classification standards are shown in Table 1. Each type can be added with "\" or "3" to indicate heavy or light, which is used to indicate the degree of resistance of wheat varieties to mold. For each identification material, 50 leaves are randomly surveyed, and the infection type is recorded leaf by leaf. When different infection types are found on the same leaf, the main infection type is recorded first. Table 1 Wheat stripe rust infection type level and symptom description Infection type
Leaf [does not produce any visible symptoms
Leaf produces small sticky substances, does not secrete symptom-free
Drilled spots or spots appear on the leaves, which are very small and rarely seen in a few days NY/T 1443.1—2007
Infection type
Table 1 (Continued)
Symptoms
Summer spores are small to medium in size, less in number, and their leaf tissues are dead or significantly chlorotic. Summer spores are large and more numerous, and their surrounding leaf tissues are chlorotic. Summer spores are large and numerous, and their surrounding edges do not fade.
Note: The infection type level is finely divided by the following symbols, that is, "" indicates that the spores are slightly smaller than the corresponding summer spores of the normal infection type; \+" indicates that the spores are slightly larger than the corresponding normal infection type. Commercial resistance (11H)
4.6.3.2 Severity record and standard
Medium resistance (MI)
China (M)
Figure 2 Grading chart of infection types of wheat stripe rust in the adult stage High susceptibility (H5)
The severity is expressed by grading method, with eight levels of 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. During the investigation, 50 leaves were randomly sampled from each material, the severity was recorded leaf by leaf, and the average severity was calculated. s(%)
X,×100%
Among them, is the average severity, is the disease level (1~n), x, is the number of units with the disease level:, S is the severity value of the disease level (such as the percentage of each level of wheat stripe rust), 4.6.3.3 Prevalence record and standard
50 leaves were randomly surveyed for each identification material Leaves, count the number of diseased leaves, and calculate the prevalence. Prevalence (%) = (number of diseased leaves/total number of leaves surveyed) × 100% 4.7 Resistance rating
4.7.1 Identification validity judgment
When the severity of disease in susceptible or highly susceptible control varieties in the identification field reaches more than 60%, the stripe rust resistance identification of this batch is considered valid. 4.7.2 Repeat identification
Materials that show high resistance or medium resistance in the initial identification need to be repeated with the same pathogen in the following year. 4.7.3 Resistance evaluation criteria
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified material. The evaluation criteria are shown in Table 2. If the results of the two years of identification are inconsistent, the degree of disease with weak resistance shall prevail. If there is a clear difference in resistance and susceptibility in an identification group, it shall be noted in the index table as "resistance separation", and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immune (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slcowrusting (SR)
Moderately wetible (vIS)Highly Sixeccptilale (HS)Original records of wheat stripe rust resistance identification and fruiting records are shown in Appendix NY/T 1443.1—2007
1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogens and physiological races of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccac, Pucxinia. The hyphae are filamentous and septate. They grow between host cells and use haustoria to enter wheat cells to absorb nutrients. They produce spores in the diseased part. Summer spores are spherical, bright yellow, with fine thorns on the surface, 32um~40um×22μm-29um in size, and 6~12 germination holes. Winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the septum, 36um~68jum×12m~20m in size, and short stalks. The pathogenicity of the fungus has physiological differentiation. my country has named 32 physiological subspecies, from No. 1 to No. 32 in the strip. The physiological subspecies of the strip-induced fungus are very easy to mutate. After 1950, there have been many changes in the dominant subspecies. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):2 Survey methods and items
During the survey, 50 leaves (mainly flag leaves) were randomly sampled from each material, and the severity and infection type were recorded leaf by leaf. The prevalence, average severity and disease index were calculated. The food was adjusted twice with an interval of 10 days. 4.6.3 Recording and standards of disease severity
4.6.3.1 Recording and standards of infection type
The infection type of the lesion is divided into six types: 0.0;, 1, 2, 3, and 4. The classification standards are shown in Table 1. Each type can be added with "\" or "three" to indicate heavy or light, which is used to indicate the degree of resistance of wheat to mold. For each identification material, 50 leaves were randomly surveyed, and the infection type was recorded leaf by leaf. When different infection types were found on the same leaf, the main infection type was recorded first. Table 1 Infection type level and symptom description of wheat stripe rust Infection type
Leaf [does not produce any visible symptoms
Leaf produces small sticky rust, does not secrete rust. Symptom-free
Dead rust spots or spots appear on the leaves, the rust is very small, rarely seen in a few days NY/T 1443.1—2007
Infection type
Table 1 (Continued)
Symptoms
Summer spores are small to medium in size, less in number, and their leaf tissues are dead or significantly chlorotic. Summer spores are large and more numerous, and their surrounding leaf tissues are chlorotic. Summer spores are large and numerous, and their surrounding edges do not fade.
Note: The infection type level is finely divided by the following symbols, that is, "" indicates that the spores are slightly smaller than the corresponding summer spores of the normal infection type; \+" indicates that the spores are slightly larger than the corresponding normal infection type. Commercial resistance (11H)
4.6.3.2 Severity record and standard
Medium resistance (MI)
China (M)
Figure 2 Grading chart of infection types of wheat stripe rust in the adult stage High susceptibility (H5)
The severity is expressed by grading method, with eight levels of 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. During the investigation, 50 leaves were randomly sampled from each material, the severity was recorded leaf by leaf, and the average severity was calculated. s(%)
X,×100%
Among them, is the average severity, is the disease level (1~n), x, is the number of units with the disease level:, S is the severity value of the disease level (such as the percentage of each level of wheat stripe rust), 4.6.3.3 Prevalence record and standard
50 leaves were randomly surveyed for each identification material Leaves, count the number of diseased leaves, and calculate the prevalence. Prevalence (%) = (number of diseased leaves/total number of leaves surveyed) × 100% 4.7 Resistance rating
4.7.1 Identification validity judgment
When the severity of disease in susceptible or highly susceptible control varieties in the identification field reaches more than 60%, the stripe rust resistance identification of this batch is considered valid. 4.7.2 Repeat identification
Materials that show high resistance or medium resistance in the initial identification need to be repeated with the same pathogen in the following year. 4.7.3 Resistance evaluation criteria
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified material. The evaluation criteria are shown in Table 2. If the results of the two years of identification are inconsistent, the degree of disease with weak resistance shall prevail. If there is a clear difference in resistance and susceptibility in an identification group, it shall be noted in the index table as "resistance separation", and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immune (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slcowrusting (SR)
Moderately wetible (vIS)Highly Sixeccptilale (HS)Original records of wheat stripe rust resistance identification and fruiting records are shown in Appendix NY/T 1443.1—2007
1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogens and physiological races of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccac, Pucxinia. The hyphae are filamentous and septate. They grow between host cells and use haustoria to enter wheat cells to absorb nutrients. They produce spores in the diseased part. Summer spores are spherical, bright yellow, with fine thorns on the surface, 32um~40um×22μm-29um in size, and 6~12 germination holes. Winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the septum, 36um~68jum×12m~20m in size, and short stalks. The pathogenicity of the fungus has physiological differentiation. my country has named 32 physiological subspecies, from No. 1 to No. 32 in the strip. The physiological subspecies of the strip-induced fungus are very easy to mutate. After 1950, there have been many changes in the dominant subspecies. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9bzxZ.net
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):2 Survey methods and items
During the survey, 50 leaves (mainly flag leaves) were randomly sampled from each material, and the severity and infection type were recorded leaf by leaf. The prevalence, average severity and disease index were calculated. The food was adjusted twice with an interval of 10 days. 4.6.3 Recording and standards of disease severity
4.6.3.1 Recording and standards of infection type
The infection type of the lesion is divided into six types: 0.0;, 1, 2, 3, and 4. The classification standards are shown in Table 1. Each type can be added with "\" or "three" to indicate heavy or light, which is used to indicate the degree of resistance of wheat to mold. For each identification material, 50 leaves were randomly surveyed, and the infection type was recorded leaf by leaf. When different infection types were found on the same leaf, the main infection type was recorded first. Table 1 Infection type level and symptom description of wheat stripe rust Infection type
Leaf [does not produce any visible symptoms
Leaf produces small sticky rust, does not secrete rust. Symptom-free
Dead rust spots or spots appear on the leaves, the rust is very small, rarely seen in a few days NY/T 1443.1—2007
Infection type
Table 1 (Continued)
Symptoms
Summer spores are small to medium in size, less in number, and their leaf tissues are dead or significantly chlorotic. Summer spores are large and more numerous, and their surrounding leaf tissues are chlorotic. Summer spores are large and numerous, and their surrounding edges do not fade.
Note: The infection type level is finely divided by the following symbols, that is, "" indicates that the spores are slightly smaller than the corresponding summer spores of the normal infection type; \+" indicates that the spores are slightly larger than the corresponding normal infection type. Commercial resistance (11H)
4.6.3.2 Severity record and standard
Medium resistance (MI)
China (M)
Figure 2 Grading chart of infection types of wheat stripe rust in the adult stage High susceptibility (H5)
The severity is expressed by grading method, with eight levels of 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. During the investigation, 50 leaves were randomly sampled from each material, the severity was recorded leaf by leaf, and the average severity was calculated. s(%)
X,×100%
Among them, is the average severity, is the disease level (1~n), x, is the number of units with the disease level:, S is the severity value of the disease level (such as the percentage of each level of wheat stripe rust), 4.6.3.3 Prevalence record and standard
50 leaves were randomly surveyed for each identification material Leaves, count the number of diseased leaves, and calculate the prevalence. Prevalence (%) = (number of diseased leaves/total number of leaves surveyed) × 100% 4.7 Resistance rating
4.7.1 Identification validity judgment
When the severity of disease in susceptible or highly susceptible control varieties in the identification field reaches more than 60%, the stripe rust resistance identification of this batch is considered valid. 4.7.2 Repeat identification
Materials that show high resistance or medium resistance in the initial identification need to be repeated with the same pathogen in the following year. 4.7.3 Resistance evaluation criteria
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified material. The evaluation criteria are shown in Table 2. If the results of the two years of identification are inconsistent, the degree of disease with weak resistance shall prevail. If there is a clear difference in resistance and susceptibility in an identification group, it shall be noted in the index table as "resistance separation", and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immune (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slcowrusting (SR)
Moderately wetible (vIS)Highly Sixeccptilale (HS)Original records of wheat stripe rust resistance identification and fruiting records are shown in Appendix NY/T 1443.1—2007
1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogens and physiological races of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccac, Pucxinia. The hyphae are filamentous and septate. They grow between host cells and use haustoria to enter wheat cells to absorb nutrients. They produce spores in the diseased part. Summer spores are spherical, bright yellow, with fine thorns on the surface, 32um~40um×22μm-29um in size, and 6~12 germination holes. Winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the septum, 36um~68jum×12m~20m in size, and short stalks. The pathogenicity of the fungus has physiological differentiation. my country has named 32 physiological subspecies, from No. 1 to No. 32 in the strip. The physiological subspecies of the strip-induced fungus are very easy to mutate. After 1950, there have been many changes in the dominant subspecies. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):1—2007
Infection type
Table 1 (continued)
Symptom summary
Summer spores are small to medium in size, less in number, and the leaf tissue is dead or significantly chlorotic. Summer spores are large and more in number, and the surrounding leaf tissue is chlorotic. Summer spores are large and numerous, and the surrounding leaf tissue is not chlorotic.
Note: The infection type level is finely divided by the following symbols, that is, "" indicates that the spores are slightly smaller than the corresponding summer spores of the normal infection type; \+" indicates that the spores are slightly larger than the corresponding normal infection type. Commercial resistance (11H)
4.6.3.2 Severity record and standard
Medium resistance (MI)
China (M)
Figure 2 Grading chart of infection types of wheat stripe rust in the adult stage High susceptibility (H5)
The severity is expressed by grading method, with eight levels of 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. During the investigation, 50 leaves were randomly sampled from each material, the severity was recorded leaf by leaf, and the average severity was calculated. s(%)
X,×100%
Among them, is the average severity, is the disease level (1~n), x, is the number of units with the disease level:, S is the severity value of the disease level (such as the percentage of each level of wheat stripe rust), 4.6.3.3 Prevalence record and standard
50 leaves were randomly surveyed for each identification material Leaves, count the number of diseased leaves, and calculate the prevalence. Prevalence (%) = (number of diseased leaves/total number of leaves surveyed) × 100% 4.7 Resistance rating
4.7.1 Identification validity judgment
When the severity of disease in susceptible or highly susceptible control varieties in the identification field reaches more than 60%, the stripe rust resistance identification of this batch is considered valid. 4.7.2 Repeat identification
Materials that show high resistance or medium resistance in the initial identification need to be repeated with the same pathogen in the following year. 4.7.3 Resistance evaluation criteria
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified material. The evaluation criteria are shown in Table 2. If the results of the two years of identification are inconsistent, the degree of disease with weak resistance shall prevail. If there is a clear difference in resistance and susceptibility in an identification group, it shall be noted in the index table as "resistance separation", and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immune (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slcowrusting (SR)
Moderately wetible (vIS)Highly Sixeccptilale (HS)Original records of wheat stripe rust resistance identification and fruiting records are shown in Appendix NY/T 1443.1—2007
1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogens and physiological races of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccac, Pucxinia. The hyphae are filamentous and septate. They grow between host cells and use haustoria to enter wheat cells to absorb nutrients. They produce spores in the diseased part. Summer spores are spherical, bright yellow, with fine thorns on the surface, 32um~40um×22μm-29um in size, and 6~12 germination holes. Winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the septum, 36um~68jum×12m~20m in size, and short stalks. The pathogenicity of the fungus has physiological differentiation. my country has named 32 physiological subspecies, from No. 1 to No. 32 in the strip. The physiological subspecies of the strip-induced fungus are very easy to mutate. After 1950, there have been many changes in the dominant subspecies. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):1—2007
Infection type
Table 1 (continued)
Symptom summary
Summer spores are small to medium in size, less in number, and the leaf tissue is dead or significantly chlorotic. Summer spores are large and more in number, and the surrounding leaf tissue is chlorotic. Summer spores are large and numerous, and the surrounding leaf tissue is not chlorotic.
Note: The infection type level is finely divided by the following symbols, that is, "" indicates that the spores are slightly smaller than the corresponding summer spores of the normal infection type; \+" indicates that the spores are slightly larger than the corresponding normal infection type. Commercial resistance (11H)
4.6.3.2 Severity record and standard
Medium resistance (MI)
China (M)
Figure 2 Grading chart of infection types of wheat stripe rust in the adult stage High susceptibility (H5)
The severity is expressed by grading method, with eight levels of 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. During the investigation, 50 leaves were randomly sampled from each material, the severity was recorded leaf by leaf, and the average severity was calculated. s(%)
X,×100%
Among them, is the average severity, is the disease level (1~n), x, is the number of units with the disease level:, S is the severity value of the disease level (such as the percentage of each level of wheat stripe rust), 4.6.3.3 Prevalence record and standard
50 leaves were randomly surveyed for each identification material Leaves, count the number of diseased leaves, and calculate the prevalence. Prevalence (%) = (number of diseased leaves/total number of leaves surveyed) × 100% 4.7 Resistance rating
4.7.1 Identification validity judgment
When the severity of disease in susceptible or highly susceptible control varieties in the identification field reaches more than 60%, the stripe rust resistance identification of this batch is considered valid. 4.7.2 Repeat identification
Materials that show high resistance or medium resistance in the initial identification need to be repeated with the same pathogen in the following year. 4.7.3 Resistance evaluation criteria
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified material. The evaluation criteria are shown in Table 2. If the results of the two years of identification are inconsistent, the degree of disease with weak resistance shall prevail. If there is a clear difference in resistance and susceptibility in an identification group, it shall be noted in the index table as "resistance separation", and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immune (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slcowrusting (SR)
Moderately wetible (vIS)Highly Sixeccptilale (HS)Original records of wheat stripe rust resistance identification and fruiting records are shown in Appendix NY/T 1443.1—2007
1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogens and physiological races of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccac, Pucxinia. The hyphae are filamentous and septate. They grow between host cells and use haustoria to enter wheat cells to absorb nutrients. They produce spores in the diseased part. Summer spores are spherical, bright yellow, with fine thorns on the surface, 32um~40um×22μm-29um in size, and 6~12 germination holes. Winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the septum, 36um~68jum×12m~20m in size, and short stalks. The pathogenicity of the fungus has physiological differentiation. my country has named 32 physiological subspecies, from No. 1 to No. 32 in the strip. The physiological subspecies of the strip-induced fungus are very easy to mutate. After 1950, there have been many changes in the dominant subspecies. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):3 Resistance evaluation standards
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified materials. The evaluation standards are shown in Table 2. If the identification results of the two years are inconsistent, the disease severity of the weaker resistance shall prevail. If there are obvious differences in resistance and susceptibility in an identified population, "resistance separation" shall be noted in the evaluation table, and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immine (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slowly rusting (SR)
Moderately wetible (vIS)Highly Sixeccptillale (HS)Original records of wheat resistance to stripe rust and fruiting record form are shown in Appendix NY/T 1443.1—2007
degree.1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogen and physiological species of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccacaceae, Pucxinia. The hyphae are filamentous and septate, growing between host cells, using haustoria to enter wheat cells to absorb nutrients, and producing spores in the diseased part. The summer spores are spherical, bright yellow, with fine spines on the surface, 32um~40um×22μm-29um in size, and have 6~12 germination holes. The winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the separation, 36um~68um×12m~20m in size, with a short stalk. The pathogenicity of the fungus shows physiological differentiation. my country has named 32 physiological races, namely, No. 1 to No. 32. The physiological races of the fungus are very easy to mutate, and there have been many changes in the dominant race since 1950. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):3 Resistance evaluation standards
The resistance level to stripe rust is determined based on the disease severity (disease index and infection type) of the identified materials. The evaluation standards are shown in Table 2. If the identification results of the two years are inconsistent, the disease severity of the weaker resistance shall prevail. If there are obvious differences in resistance and susceptibility in an identified population, "resistance separation" shall be noted in the evaluation table, and the proportion shall be separated by "". Table 2 Evaluation criteria for resistance to stripe rust
Infection type
Disease cycle index
Immine (I)
Resistance evaluation
Nearly immune (NIM))
Infection type
Identification record form
Disease index
Table 2 (continued)
NY/T 1443.1—2007
Resistance evaluation
Highly Rcsistani (HR)
Moclerately resistent (MR)Slowly rusting (SR)
Moderately wetible (vIS)Highly Sixeccptillale (HS)Original records of wheat resistance to stripe rust and fruiting record form are shown in Appendix NY/T 1443.1—2007
degree.1 Scientific name and morphological description
Appendix A
(Informative Appendix)
Pathogen and physiological species of wheat stripe rust
Puccinia striifarmis Wcst, f.sp, tritici Eriks.ct Hern belongs to Fungi, Basidiomycotina, Urediniamyeetes, Puccinaccacaceae, Pucxinia. The hyphae are filamentous and septate, growing between host cells, using haustoria to enter wheat cells to absorb nutrients, and producing spores in the diseased part. The summer spores are spherical, bright yellow, with fine spines on the surface, 32um~40um×22μm-29um in size, and have 6~12 germination holes. The winter spores are twin, club-shaped, flat or oblique at the front, slightly constricted at the separation, 36um~68um×12m~20m in size, with a short stalk. The pathogenicity of the fungus shows physiological differentiation. my country has named 32 physiological races, namely, No. 1 to No. 32. The physiological races of the fungus are very easy to mutate, and there have been many changes in the dominant race since 1950. A.2 Identification of Physiological Races
A.2.1 Hosts Used for Identification
The hosts used for identification of physiological races were TrigoEureka, Fulhard, Baochun 128 (1utescens 128), Mentana 2419 (Mentana), Virgilio, Abbondanza, Early Prcmium, Funo, Daxish 1, Jubilejina 2, Fengchan 3, Iovrin 13, Kangyin 655, Shuiyuan 11, 7hong 4, Lovrin 10, Hybrid 46, Triticuan spetta album and Guinong 22. 22) There are 19 varieties in total.
A.2.2 Coding rules and naming of physiological races of stripe rust The physiological races and pathotypes of Chinese wheat stripe rust and their responses in host identification are shown in Table A.1. NY/T1443.1—2007
Medium tea number
Medium strip number
Zhong No. 8
Lizhong No. 5
ertirr
Zhongshao No.
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang "9
NY/T1443.1—2007
No. Variety name
Note 1: Identification location
Note 2: Inoculated pathogen isolate abbreviation
Note 3; According to the date of planting Note 4: Sowing height
Note 5: Height
[Normative Appendix]
Original record and result record table of identification of wheat varieties (lines) against stripe rust in 2017 Epidemic level
Severity
[100! Average
Common rate %
Average incidence
Number of leaves
Physiological subspecies type
Survey date
Checking point
Number of leaves
Adjustment result
Deviation index
Identification technical person in charge (signed by Ning):1—2007
Tea Middle Number
Strip Middle Number
Zhong No. 8
Lizhong No. 5
ertirr
Strip Middle Number
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang“9
NY/T1443.1—2007
No. Variety Name
Note 1: Identification Location
Note 2: Abbreviation of Inoculated Pathogen Isolate
Note 3; By Species Date||tt ||Note 4: Sowing height
Note 5: Height
【Normative Appendix】
Original record and result record table of identification of wheat varieties (lines) with resistance to stripe rust in 2017 Epidemic level
Severity
[100！ Average
Common rate%
Average incidence
Number of leaves
Physiological subspecies type
Investigation date
Investigation point
Number of leaves
Setting result
Deviation index
Identification technical personnel (signed by Ning):1—2007
Tea Middle Number
Strip Middle Number
Zhong No. 8
Lizhong No. 5
ertirr
Strip Middle Number
Rong No. 4
Caizhong No. 2
Chazhong No. 2
Taizhong No. 18
Bang“9
NY/T1443.1—2007
No. Variety Name
Note 1: Identification Location
Note 2: Abbreviation of Inoculated Pathogen Isolate
Note 3; By Species Date||tt ||Note 4: Sowing height
Note 5: Height
【Normative Appendix】
Original record and result record table of identification of wheat varieties (lines) with resistance to stripe rust in 2017 Epidemic level
Severity
[100！ Average
Common rate%
Average incidence
Number of leaves
Physiological subspecies type
Investigation date
Investigation point
Number of leaves
Setting result
Deviation index
Identification technical personnel (signed by Ning):
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