Pesticide guidelines for the field efficacy trials—Part 35:Weed control of herbicides in direct seeding vegetable fields
Some standard content:
ICS 65.100
Agricultural Industry Standard of the People's Republic of China
NY/T 1464.35-2010
Pesticide guidelines fur the field efficacy trialsPart 35 : Wced control of nerbicides in direct seeding vegetable fields2010-05-20 Released
2010-09-01 Implementation
The Ministry of Agriculture of the People's Republic of China released
NY/T1464 "Test Methods for the Effectiveness of Pesticides" is a series of standards, totaling 36 parts: Part 1: Anti-locusts for Controlling Migratory Locusts;
Part 2: Tea Insecticides for Controlling Rice Water Bugs; Part 3: Mountain Insecticides for Controlling Elliptic Stink Bugs;
Part 4: Insecticides for Controlling Pear Yellow Aphids; Part 5: Insecticides for Controlling Apple Aphids; Part 6: Tea Insecticides for Controlling Vegetable Thrips; Part 7 Part: Fungicides for the control of tobacco charcoal hernia; Part 8: Fungicides for the control of tomato virus diseases: Part 9: Fungicides for the control of pepper tender virus diseases; Part 10: Fungicides for the control of mushroom bubble disease; Part 11: Tea fungus for the control of banana Xiken disease; Part 12: Tea fungus for the control of grape powdery mildew; Part 13: Fungicides for the control of grape chrysanthemum anthracnose; Part 14: Fungicides for the control of water wilt; Part 1.5: Tea fungus for the control of wheat red rain disease; Part 16: Fungicides for the control of wheat root rot; Part 17: Herbicides for the control of green bean mouth weeds; Part 18: herbicides for controlling weeds in sesame fields; Part 19: herbicides for controlling weeds in wolfberry fields; Part 20: herbicides for controlling weeds in tomatoes; Part 21: herbicides for controlling weeds in cucumber fields; Part 22: herbicides for controlling weeds in Renmiao fields; Part 23: herbicides for controlling weeds in Shoujiri; Part 24: herbicides for controlling weeds in red beans; Part 25: herbicides for controlling weeds in tobacco seedlings; Part 26: test for cotton wilt accelerators:
Part 27: insecticides for controlling cruciferous vegetables Aphids, Part 28: pesticides for broad-leaved tree longhorn beetles; Part 29: insecticides for loose-coupled longhorn beetles; Part 30: fungicides for tobacco angular spot disease; Part 31: tea fungus for ginger blight; Part 32: fungicides for solanum bacterial wilt; Part 33: fungicides for rainbow spot rust; Part 31: fungicides for finger yellow water chestnut disease; Part 35: herbicides for direct-seeded vegetable Ⅱ weeds; Part 36: herbicides for pineapple weeds. This part is Part 5 of the "Guidelines for Field Efficacy Tests of Pesticides". NY/T 1464.35—2010
NY/T1464.35—2010
This part is for export to the Ministry of Agriculture of the People's Republic of China. Drafting unit of this part: Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this part are: Zhang Hongge, Zhang Wei, Nie Dongxing, Chen Liping, Liu Yixue, Cui Hailan, Lu Xingtao 1 Scope
Guidelines for field efficacy tests of pesticides
NY/T 1464.35—2010
Part 35: Herbicide control of weeds in direct-seeded vegetable fields The small part specifies the methods and basic requirements for field efficacy plot tests of herbicides for controlling weeds in direct-seeded vegetables. This part is applicable to the field efficacy plot tests and efficacy evaluation of herbicides for controlling weeds in direct-seeded vegetables. Other direct-seeded vegetable efficacy tests can be used as a reference.
2 Test conditions
2.1 Vegetables and cultivars
Record the species and cultivation type. Select widely planted conventional vegetable varieties or vegetable varieties required by the agreement, and record the vegetable variety name, germination rate and sowing time.
2.2 Selection of test weeds
The test site must have representative weeds. The weed species must be consistent with the killing spectrum of the herbicide to be tested. The distribution must be uniform and the weed density must meet the test needs. The leaf names and Latin names of various weeds should be recorded: 2.3 Cultivation conditions
The soil conditions (soil type, organic matter content, pH value, soil quality, fertility, etc.) and tillage measures of all test plots must be consistent and in line with local scientific agricultural practices (GAP). The sowing period, sowing amount, sowing depth, row spacing and plant spacing of vegetables should be in line with local production practices.
Record the previous crop and the herbicide used before. Avoid choosing herbicides that have been used before and affect the growth of vegetables or the effect of the test agent. If there is irrigation, record the irrigation time, water volume and method.3 Experimental design and arrangement
3.1 Chemical
3.1.1 Test Chemical
The test chemical treatment is set at high, medium, low and multiple of medium, with a total of 4 doses (multiple of medium is set to evaluate the safety of the chemical on vegetables) or the drug dosage specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). Record the chemical name (in Chinese, English) or code, dosage form, content, manufacturer and treatment dosage (expressed in effective concentration ng/kg or mg/L)3.1.2 Control Chemical
The control chemical should be a product that has been registered and proven to have good safety and herbicidal effect in practice. Its type and mode of action should be similar to that of the test chemical: the control chemical should be used at the local conventional application rate. Special circumstances can be determined according to the test month. If the test agent is a single agent, at least one local area should be used as a single agent as a control agent; when the test agent is a mixture, each single agent and the local commonly used agent should be used as a control agent.
Record the generic name, dosage form, content, manufacturer, and application amount of the control agent: 3.2 Plot Arrangement
3.2.1 Plot Arrangement
The treatment plots of the test agent, control agent, blank control, and manual weeding control are arranged in random blocks. In special cases, such as the experiment of perennial weed removal, in order to avoid the disturbance caused by uneven distribution of weeds, the plots can be arranged irregularly according to the actual situation, and the plots can be arranged irregularly according to the actual situation, and the plots can be arranged irregularly according to the actual situation, and the plots can be arranged irregularly according to the actual situation. 1
NY/T1464.35-2010
3.2.2 Plot area and repetition
Plot area: 1.5m2~3iz for densely planted vegetables. Repetition number: at least 4 repetitions.
3.3 Application method
3.3.1 Usage method
Follow the requirements of the agreement and the instructions on the label, and the penetration method is usually used. The application method should be in line with the actual local agricultural production. 3.3.2 Application equipment
Select a sprayer with stable pressure and fan-shaped nozzle for application to ensure that the pesticide is evenly distributed. Distribute to the designated application site of the test, record all information such as the type of equipment used and operating conditions (operating pressure, nozzle type and height, nozzle diameter, mixing depth), etc.: The dosage should be accurate, and any deviation of the dosage exceeding 10% should be recorded, and the factors that affect the efficacy, duration and selectivity of the weed control seal should be recorded. 3.3.3 Application time and frequency
Follow the requirements of the agreement and the instructions on the label. The application time is related to the emergence time of weeds and vegetables: a) Before sowing vegetables (mixed with soil or not); h) After sowing vegetables to before emergence (mixed with soil or not); 3) After vegetable seedlings.
Record the date and time of each application, as well as the growth status of weeds and vegetables (seedlings, growth period) at the time of application. If the application time is indicated on the label (or If there is no special note on the protocol, the pesticide should be applied according to the characteristics of the test agent. The same agent can be used once or in divided doses, and the number of applications and time should be recorded. 3.3.4 Application amount and water consumption
Apply the pesticide according to the requirements of the protocol and the dosage indicated on the label. Usually the dosage of the pesticide is expressed in active ingredient/hm, and the water consumption is expressed in I./hm. When the amount of water is not specified in the protocol, the amount of water can be determined based on the mode of action of the test agent, the type of sprayer, and local experience.
3.3.5 Agents for controlling pests and diseases and non-target weeds If other agents are used, agents that have no effect on the test agent, the objects to be controlled and the vegetables should be selected, and all plots should be treated evenly, and the test drugs should be used together. The pesticide and control pesticide should be used separately to minimize the interference of these pesticides. Record the exact number of pesticides applied (such as pesticide name, application time, dosage, etc.). 4 Survey records and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
Record the temperature on the day of application and the previous 10 days (average temperature, maximum and minimum temperature, expressed in °C), rainfall (type and rainfall amount, expressed in mm), wind force, cloudy weather and other meteorological data. All these meteorological data can be recorded at the test site or obtained at a weather station near the test site.
Record severe weather during the entire test period, such as severe rain or continuous drought, heavy rain, late cold, ice, etc. 4.1.2 Soil data
Record soil type (record its composition as much as possible), organic matter content, soil fertility, pH value, soil moisture (mixing degree, water accumulation), etc. 4.2 Management
If there are agricultural activities such as fertilization, irrigation, disease prevention and control, etc., the time, frequency and method should be recorded. Recorder! Covering time and number of weeding plots, etc. 4.3 Weed survey
4.3.1 Survey method
NY/T 1464.35—2010
Detailed description of the symptoms of weed damage (such as growth inhibition, chlorosis, deformity, etc.) to accurately explain the mode of action of the pesticide Record the weed population in the plot, such as weed species, number of weeds, coverage or weed weight, etc., using the following absolute value method or estimated value a) Absolute value (number measurement) survey method
Calculate the number or weight of weeds, conduct a survey of the entire plot or randomly select 3~4 points in each plot, and conduct a sample survey of 0.25m1.00㎡ square blocks at each point. In some cases, special organs of the weed plot (such as the number of monocotyledonous weeds) are investigated.
b) Estimated value (visual observation) survey method.
Each pesticide-treated area is compared with the adjacent blank control area to estimate the relative weed population. This survey method includes a survey of weed communities and weed species, and can be measured by weed number, coverage, height and growth potential (such as actual weed volume). The results can be expressed as a simple percentage (0 means no weeds, 100% means the same weeds as the blank control area), or converted to express the percentage effect of weed control (0 means no control effect, 1% means all weeds are controlled). The absolute value of the number of weeds in the blank control area should also be recorded. In order to overcome the difficulties of accurately estimating percentages and making variances homogeneous, the following grading standards can be used for investigation: Grade 1: no weeds; Grade 2: equivalent to 0-2.5% of the blank control area; Grade 3: equivalent to 2.6%~5% of the blank control area; Grade 2: equivalent to 5.1%~10% of the solid film control area; Grade 5: equivalent to the blank film area 10.1%~1% of the blank control area: 6th level: equivalent to 15.1%~25% of the blank control area; 7th level: equivalent to 25.1%~35% of the blank control area; 8th level: equivalent to 35.1%~67.5% of the blank control area; 9th level: equivalent to 67.6%~100% of the research area. Investigators must be trained before using this grading standard. This grading standard can be used directly without converting it into an average value of the estimated value percentage.
4.3.2 Survey time and frequency
The survey time varies according to the type of vegetable cultivation, the characteristics of the pesticide and the time of application. The specific adjustment times are as follows: a) Apply pesticides before vegetable sowing or before seedlings emerge after sowing. First survey: after weeds emerge in the control area (generally 10-15 days after application in summer and autumn, 20-30 days after application in winter).
First adjustment: during the vigorous growth period of weeds in the control area, about 1 month after application in spring, summer and autumn, and about 60 days after application in winter).
Third survey: after new weeds emerge in the treatment area (generally 45-60 days after application in summer and autumn, and 75-90 days after application in winter).
Due to the length of the growth period of root vegetables, it may be considered to conduct another adjustment before harvesting vegetables. 1) Application of pesticides in the seedling stage of vegetables
First adjustment: before application of pesticides. Investigate the types and growth period of weeds, as well as the self-proportion of the main weeds (base survey). Second survey: 10-30 days after application (generally 10 days after application in spring, summer and autumn, and 20-30 days after application in winter) (quick-acting survey). Third survey: conducted when the weed control effect is the best. Fourth survey: conducted after new weeds sprout in the treatment area or before vegetable harvest (lasting effect survey). 4.3.3 Calculation method of control effect
Before sowing and before seedling after sowing and the last adjustment of post-seedling application, the fresh weight or plant height of each buried control effect is calculated according to formula (1), and the control effect of post-seedling application except the last investigation is calculated according to formula (2): 3
NY/T 1464. 35--2010
Where:
E-control effect.%
C-number of live weeds in the blank control area (or fresh);
T-number of remaining weeds in the treatment area (or fresh weight); X,-number of weeds in the treatment area before application; X...number of remaining weeds in the treatment area after application. The calculation result shall be rounded to two decimal places.
4.4 Crop Survey
4.4.1 Survey Method
E=X=×i×100
..........
...-+-..(2)
Observe whether the pesticide has any effect on the growth of the crops. If there is any, record the type and extent of the damage. The following requirements can be followed: a) Record the time and symptoms of the damage in detail (such as growth inhibition, chlorosis, sticky spots, deformity, etc.). At the same time, observe the interaction between the damage and the adverse factors (cultivation methods, inversion, disease invasion, long-term high temperature or freezing damage, etc.). b) If the damage can be counted or measured, measure the absolute value, such as the rate of damaged plants or plant height. d) In other cases, the extent and rate of damage can be estimated by the following two methods. 1) Compare the pesticide-treated area with the control area to evaluate the pesticide damage rate. 2) According to the pesticide damage grading method, grade and score the pesticide damage in each plot: Level 1: normal growth, no symptoms; Level 2: no obvious pesticide damage, the symptom rate is less than 10%, and the yield is not affected; Level 3: slight pesticide damage, which can be recovered, and the yield is not affected; Level 4: moderate pesticide damage, difficult to recover, resulting in a significant reduction in yield; Level 5: severe pesticide damage, which cannot be recovered, resulting in a serious reduction in yield or no yield. 4.4.2 Survey time and frequency
Please check the time and frequency according to the pesticide application. The actual survey frequency is as follows: a) Apply pesticides (soil treatment agents) before sowing or before seedlings emerge after sowing. The first survey: Conducted after all the seedlings in the control area emerge. Observe whether there is delayed emergence of seedlings in the treatment area, use the absolute number adjustment method to investigate the seedling situation, and count the number of seedlings. The second investigation: Conduct it during the vigorous growth period of the control vegetables or the period when the test agent is most effective, and observe for any signs of drug damage. If there is drug damage, carefully record the symptoms of drug damage, the damage situation and the time required for the vegetables to return to normal growth. At the same time, use the absolute number adjustment method to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. The third investigation: Conduct sample measurement before the vegetable is harvested. b) Apply the drug after emergence (purpurinol treatment agent). Observe for drug damage at 3d, 5d, 7a, 10d and 15d after application. If there is drug damage, the degree of drug damage should be recorded in detail, and the time required for the vegetables to return to normal growth should be observed and recorded. If there is drug damage, the absolute value method should be used when the damage symptoms are most obvious to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. Before harvesting vegetables, take samples to measure yield.
4.5 Yield and quality survey
The yield measurement area of each test plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches. The yield of the plot is the sum of the yield of each batch. Yield is usually expressed in kg/hm2. If required, the quality of vegetables should also be assessed according to relevant standards. 6 Side effect observation
Record the impact on non-target organisms.
5 Results
NY/T1464.35—2010
The results obtained in the test were analyzed using biological statistical methods, and a test report was written in a formal format, and the test results were analyzed and evaluated. The original data should be kept for examination and verification. If the pesticide shows signs of long-lasting effectiveness in the test, subsequent crop safety tests should be carried out.00㎡ square block for sampling survey. In some cases, special organs of the weed patch (such as the number of monocotyledonous weeds) are investigated.
b) Estimated value (visual inspection) survey method.
Each pesticide treatment area is compared with the adjacent blank control area to estimate the relative weed population. This survey method includes weed community and weed species investigation, and can be measured by weed number, coverage, height and growth potential (such as actual weed quantity). The results can be expressed as a simple percentage (0 means no weeds, 100% means the same weeds as the blank control area), or converted to express the percentage effect of weed control (0 means no control effect, 1% means all weeds are controlled). The absolute value of the number of weeds in the blank control area should also be recorded. In order to overcome the difficulties of accurately estimating percentages and making variances homogeneous, the following grading standards can be used for investigation: Grade 1: no weeds; Grade 2: equivalent to 0-2.5% of the blank control area; Grade 3: equivalent to 2.6%~5% of the blank control area; Grade 2: equivalent to 5.1%~10% of the solid film control area; Grade 5: equivalent to the blank film area 10.1%~1% of the blank control area: 6th level: equivalent to 15.1%~25% of the blank control area; 7th level: equivalent to 25.1%~35% of the blank control area; 8th level: equivalent to 35.1%~67.5% of the blank control area; 9th level: equivalent to 67.6%~100% of the research area. Investigators must be trained before using this grading standard. This grading standard can be used directly without converting it into an average value of the estimated value percentage.
4.3.2 Survey time and frequency
The survey time varies according to the type of vegetable cultivation, the characteristics of the pesticide and the time of application. The specific adjustment times are as follows: a) Apply pesticides before vegetable sowing or before seedlings emerge after sowing. First survey: after weeds emerge in the control area (generally 10-15 days after application in summer and autumn, 20-30 days after application in winter).
First adjustment: during the vigorous growth period of weeds in the control area, about 1 month after application in spring, summer and autumn, and about 60 days after application in winter). Www.bzxZ.net
Third survey: after new weeds emerge in the treatment area (generally 45-60 days after application in summer and autumn, and 75-90 days after application in winter).
Due to the length of the growth period of root vegetables, it may be considered to conduct another adjustment before harvesting vegetables. 1) Application of pesticides in the seedling stage of vegetables
First adjustment: before application of pesticides. Investigate the types and growth period of weeds, as well as the self-proportion of the main weeds (base survey). Second survey: 10-30 days after application (generally 10 days after application in spring, summer and autumn, and 20-30 days after application in winter) (rapid-acting product survey). Third survey: conducted when the weed control effect is the best. Fourth survey: conducted after new weeds sprout in the treatment area or before vegetable harvest (lasting effect survey). 4.3.3 Calculation method of control effect
Before sowing and before seedling after sowing and the last adjustment of post-seedling application, the fresh weight or plant height of each buried control effect is calculated according to formula (1), and the control effect of post-seedling application except the last investigation is calculated according to formula (2): 3
NY/T 1464. 35--2010
Where:
E-control effect.%
C-number of live weeds in the blank control area (or fresh);
T-number of remaining weeds in the treatment area (or fresh weight); X,-number of weeds in the treatment area before application; X...number of remaining weeds in the treatment area after application. The calculation result shall be rounded to two decimal places.
4.4 Crop Survey
4.4.1 Survey Method
E=X=×i×100
..........
...-+-..(2)
Observe whether the pesticide has any effect on the growth of the crops. If there is any, record the type and extent of the damage. The following requirements can be followed: a) Record the time and symptoms of the damage in detail (such as growth inhibition, chlorosis, sticky spots, deformity, etc.). At the same time, observe the interaction between the damage and the adverse factors (cultivation methods, inversion, disease invasion, long-term high temperature or freezing damage, etc.). b) If the damage can be counted or measured, measure the absolute value, such as the rate of damaged plants or plant height. d) In other cases, the extent and rate of damage can be estimated by the following two methods. 1) Compare the pesticide-treated area with the control area to evaluate the pesticide damage rate. 2) According to the pesticide damage grading method, grade and score the pesticide damage in each plot: Level 1: normal growth, no symptoms; Level 2: no obvious pesticide damage, the symptom rate is less than 10%, and the yield is not affected; Level 3: slight pesticide damage, which can be recovered, and the yield is not affected; Level 4: moderate pesticide damage, difficult to recover, resulting in a significant reduction in yield; Level 5: severe pesticide damage, which cannot be recovered, resulting in a serious reduction in yield or no yield. 4.4.2 Survey time and frequency
Please check the time and frequency according to the pesticide application. The actual survey frequency is as follows: a) Apply pesticides (soil treatment agents) before sowing or before seedlings emerge after sowing. The first survey: Conducted after all the seedlings in the control area emerge. Observe whether there is delayed emergence of seedlings in the treatment area, use the absolute number adjustment method to investigate the seedling situation, and count the number of seedlings. The second investigation: Conduct it during the vigorous growth period of the control vegetables or the period when the test agent is most effective, and observe for any signs of drug damage. If there is drug damage, carefully record the symptoms of drug damage, the damage situation and the time required for the vegetables to return to normal growth. At the same time, use the absolute number adjustment method to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. The third investigation: Conduct sample measurement before the vegetable is harvested. b) Apply the drug after emergence (purpurinol treatment agent). Observe for drug damage at 3d, 5d, 7a, 10d and 15d after application. If there is drug damage, the degree of drug damage should be recorded in detail, and the time required for the vegetables to return to normal growth should be observed and recorded. If there is drug damage, the absolute value method should be used when the damage symptoms are most obvious to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. Before harvesting vegetables, take samples to measure yield.
4.5 Yield and quality survey
The yield measurement area of each test plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches. The yield of the plot is the sum of the yield of each batch. Yield is usually expressed in kg/hm2. If required, the quality of vegetables should also be assessed according to relevant standards. 6 Side effect observation
Record the impact on non-target organisms.
5 Results
NY/T1464.35—2010
The results obtained in the test were analyzed using biological statistical methods, and a test report was written in a formal format, and the test results were analyzed and evaluated. The original data should be kept for examination and verification. If the pesticide shows signs of long-lasting effectiveness in the test, subsequent crop safety tests should be carried out.00㎡ square block for sampling survey. In some cases, special organs of the weed patch (such as the number of monocotyledonous weeds) are investigated.
b) Estimated value (visual inspection) survey method.
Each pesticide treatment area is compared with the adjacent blank control area to estimate the relative weed population. This survey method includes weed community and weed species investigation, and can be measured by weed number, coverage, height and growth potential (such as actual weed quantity). The results can be expressed as a simple percentage (0 means no weeds, 100% means the same weeds as the blank control area), or converted to express the percentage effect of weed control (0 means no control effect, 1% means all weeds are controlled). The absolute value of the number of weeds in the blank control area should also be recorded. In order to overcome the difficulties of accurately estimating percentages and making variances homogeneous, the following grading standards can be used for investigation: Grade 1: no weeds; Grade 2: equivalent to 0-2.5% of the blank control area; Grade 3: equivalent to 2.6%~5% of the blank control area; Grade 2: equivalent to 5.1%~10% of the solid film control area; Grade 5: equivalent to the blank film area 10.1%~1% of the blank control area: 6th level: equivalent to 15.1%~25% of the blank control area; 7th level: equivalent to 25.1%~35% of the blank control area; 8th level: equivalent to 35.1%~67.5% of the blank control area; 9th level: equivalent to 67.6%~100% of the research area. Investigators must be trained before using this grading standard. This grading standard can be used directly without converting it into an average value of the estimated value percentage.
4.3.2 Survey time and frequency
The survey time varies according to the type of vegetable cultivation, the characteristics of the pesticide and the time of application. The specific adjustment times are as follows: a) Apply pesticides before vegetable sowing or before seedlings emerge after sowing. First survey: after weeds emerge in the control area (generally 10-15 days after application in summer and autumn, 20-30 days after application in winter).
First adjustment: during the vigorous growth period of weeds in the control area, about 1 month after application in spring, summer and autumn, and about 60 days after application in winter).
Third survey: after new weeds emerge in the treatment area (generally 45-60 days after application in summer and autumn, and 75-90 days after application in winter).
Due to the length of the growth period of root vegetables, it may be considered to conduct another adjustment before harvesting vegetables. 1) Application of pesticides in the seedling stage of vegetables
First adjustment: before application of pesticides. Investigate the types and growth period of weeds, as well as the self-proportion of the main weeds (base survey). Second survey: 10-30 days after application (generally 10 days after application in spring, summer and autumn, and 20-30 days after application in winter) (rapid-acting product survey). Third survey: conducted when the weed control effect is the best. Fourth survey: conducted after new weeds sprout in the treatment area or before vegetable harvest (lasting effect survey). 4.3.3 Calculation method of control effect
Before sowing and before seedling after sowing and the last adjustment of post-seedling application, the fresh weight or plant height of each buried control effect is calculated according to formula (1), and the control effect of post-seedling application except the last investigation is calculated according to formula (2): 3
NY/T 1464. 35--2010
Where:
E-control effect.%
C-number of live weeds in the blank control area (or fresh);
T-number of remaining weeds in the treatment area (or fresh weight); X,-number of weeds in the treatment area before application; X...number of remaining weeds in the treatment area after application. The calculation result shall be rounded to two decimal places.
4.4 Crop Survey
4.4.1 Survey Method
E=X=×i×100
..........
...-+-..(2)
Observe whether the pesticide has any effect on the growth of the crops. If there is any, record the type and extent of the damage. The following requirements can be followed: a) Record the time and symptoms of the damage in detail (such as growth inhibition, chlorosis, sticky spots, deformity, etc.). At the same time, observe the interaction between the damage and the adverse factors (cultivation methods, inversion, disease invasion, long-term high temperature or freezing damage, etc.). b) If the damage can be counted or measured, measure the absolute value, such as the rate of damaged plants or plant height. d) In other cases, the extent and rate of damage can be estimated by the following two methods. 1) Compare the pesticide-treated area with the control area to evaluate the pesticide damage rate. 2) According to the pesticide damage grading method, grade and score the pesticide damage in each plot: Level 1: normal growth, no symptoms; Level 2: no obvious pesticide damage, the symptom rate is less than 10%, and the yield is not affected; Level 3: slight pesticide damage, which can be recovered, and the yield is not affected; Level 4: moderate pesticide damage, difficult to recover, resulting in a significant reduction in yield; Level 5: severe pesticide damage, which cannot be recovered, resulting in a serious reduction in yield or no yield. 4.4.2 Survey time and frequency
Please check the time and frequency according to the pesticide application. The actual survey frequency is as follows: a) Apply pesticides (soil treatment agents) before sowing or before seedlings emerge after sowing. The first survey: Conducted after all the seedlings in the control area emerge. Observe whether there is delayed emergence of seedlings in the treatment area, use the absolute number adjustment method to investigate the seedling situation, and count the number of seedlings. The second investigation: Conduct it during the vigorous growth period of the control vegetables or the period when the test agent is most effective, and observe for any signs of drug damage. If there is drug damage, carefully record the symptoms of drug damage, the damage situation and the time required for the vegetables to return to normal growth. At the same time, use the absolute number adjustment method to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. The third investigation: Conduct sample measurement before the vegetable is harvested. b) Apply the drug after emergence (purpurinol treatment agent). Observe for drug damage at 3d, 5d, 7a, 10d and 15d after application. If there is drug damage, the degree of drug damage should be recorded in detail, and the time required for the vegetables to return to normal growth should be observed and recorded. If there is drug damage, the absolute value method should be used when the damage symptoms are most obvious to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. Before harvesting vegetables, take samples to measure yield.
4.5 Yield and quality survey
The yield measurement area of each test plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches. The yield of the plot is the sum of the yield of each batch. Yield is usually expressed in kg/hm2. If required, the quality of vegetables should also be assessed according to relevant standards. 6 Side effect observation
Record the impact on non-target organisms.
5 Results
NY/T1464.35—2010
The results obtained in the test were analyzed using biological statistical methods, and a test report was written in a formal format, and the test results were analyzed and evaluated. The original data should be kept for examination and verification. If the pesticide shows signs of long-lasting effectiveness in the test, subsequent crop safety tests should be carried out.2 Survey time and frequency
Survey time varies according to vegetable cultivation type, pesticide characteristics and pesticide application time. The specific survey frequency is as follows: a) Apply pesticide before vegetable sowing or before seedlings emerge after sowing. First survey: Conduct weeds in the control area after weeds emerge (generally 10-15 days after pesticide application in summer and autumn, 20-30 days after pesticide application in winter)
First survey: Conduct weeds in the control area during the vigorous growth period (about 1 month after pesticide application in spring, summer and autumn, about 60 days after pesticide application in winter).
Third survey: Conduct weeds in the pesticide treatment area after new weeds emerge (generally 45-60 days after pesticide application in summer and autumn, 75-90 days after pesticide application in winter).
The length of the growth period of root-thrown vegetables can be considered to be adjusted again before the vegetables are harvested. 1) Application of pesticides in the seedling stage of vegetables
The first adjustment: before applying pesticides. Investigate the types and growth periods of weeds, as well as the self-distribution ratio of the main weeds (base survey). The second survey: 10d~30d after the application of pesticides. In spring, summer and autumn, the survey is generally conducted 10d1s after the application of pesticides, and in winter, the survey is generally conducted 20d-~30d after the application of pesticides). The third survey: It is conducted when the weed control effect is the best. The fourth survey: It is conducted after the new weeds in the pesticide-treated area have sprouted or before the vegetables are harvested (lasting effect survey). 4.3.3 Calculation method of control effect
Before sowing and before seedling after sowing and the last adjustment of post-seedling application, the fresh weight or plant height of each buried control effect is calculated according to formula (1), and the control effect of post-seedling application except the last investigation is calculated according to formula (2): 3
NY/T 1464. 35--2010
Where:
E-control effect.%
C-number of live weeds in the blank control area (or fresh);
T-number of remaining weeds in the treatment area (or fresh weight); X,-number of weeds in the treatment area before application; X...number of remaining weeds in the treatment area after application. The calculation result shall be rounded to two decimal places.
4.4 Crop Survey
4.4.1 Survey Method
E=X=×i×100
..........
...-+-..(2)
Observe whether the pesticide has any effect on the growth of the crops. If there is any, record the type and extent of the damage. The following requirements can be followed: a) Record the time and symptoms of the damage in detail (such as growth inhibition, chlorosis, sticky spots, deformity, etc.). At the same time, observe the interaction between the damage and the adverse factors (cultivation methods, inversion, disease invasion, long-term high temperature or freezing damage, etc.). b) If the damage can be counted or measured, measure the absolute value, such as the rate of damaged plants or plant height. d) In other cases, the extent and rate of damage can be estimated by the following two methods. 1) Compare the pesticide-treated area with the control area to evaluate the pesticide damage rate. 2) According to the pesticide damage grading method, grade and score the pesticide damage in each plot: Level 1: normal growth, no symptoms; Level 2: no obvious pesticide damage, the symptom rate is less than 10%, and the yield is not affected; Level 3: slight pesticide damage, which can be recovered, and the yield is not affected; Level 4: moderate pesticide damage, difficult to recover, resulting in a significant reduction in yield; Level 5: severe pesticide damage, which cannot be recovered, resulting in a serious reduction in yield or no yield. 4.4.2 Survey time and frequency
Please check the time and frequency according to the pesticide application. The actual survey frequency is as follows: a) Apply pesticides (soil treatment agents) before sowing or before seedlings emerge after sowing. The first survey: Conducted after all the seedlings in the control area emerge. Observe whether there is delayed emergence of seedlings in the treatment area, use the absolute number adjustment method to investigate the seedling situation, and count the number of seedlings. The second investigation: Conduct it during the vigorous growth period of the control vegetables or the period when the test agent is most effective, and observe for any signs of drug damage. If there is drug damage, carefully record the symptoms of drug damage, the damage situation and the time required for the vegetables to return to normal growth. At the same time, use the absolute number adjustment method to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. The third investigation: Conduct sample measurement before the vegetable is harvested. b) Apply the drug after emergence (purpurinol treatment agent). Observe for drug damage at 3d, 5d, 7a, 10d and 15d after application. If there is drug damage, the degree of drug damage should be recorded in detail, and the time required for the vegetables to return to normal growth should be observed and recorded. If there is drug damage, the absolute value method should be used when the damage symptoms are most obvious to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. Before harvesting vegetables, take samples to measure yield.
4.5 Yield and quality survey
The yield measurement area of each test plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches. The yield of the plot is the sum of the yield of each batch. Yield is usually expressed in kg/hm2. If required, the quality of vegetables should also be assessed according to relevant standards. 6 Side effect observation
Record the impact on non-target organisms.
5 Results
NY/T1464.35—2010
The results obtained in the test were analyzed using biological statistical methods, and a test report was written in a formal format, and the test results were analyzed and evaluated. The original data should be kept for examination and verification. If the pesticide shows signs of long-lasting effectiveness in the test, subsequent crop safety tests should be carried out.2 Survey time and frequency
Survey time varies according to vegetable cultivation type, pesticide characteristics and pesticide application time. The specific survey frequency is as follows: a) Apply pesticide before vegetable sowing or before seedlings emerge after sowing. First survey: Conduct weeds in the control area after weeds emerge (generally 10-15 days after pesticide application in summer and autumn, 20-30 days after pesticide application in winter)
First survey: Conduct weeds in the control area during the vigorous growth period (about 1 month after pesticide application in spring, summer and autumn, about 60 days after pesticide application in winter).
Third survey: Conduct weeds in the pesticide treatment area after new weeds emerge (generally 45-60 days after pesticide application in summer and autumn, 75-90 days after pesticide application in winter).
The length of the growth period of root-thrown vegetables can be considered to be adjusted again before the vegetables are harvested. 1) Application of pesticides in the seedling stage of vegetables
The first adjustment: before applying pesticides. Investigate the types and growth periods of weeds, as well as the self-distribution ratio of the main weeds (base survey). The second survey: 10d~30d after the application of pesticides. In spring, summer and autumn, the survey is generally conducted 10d1s after the application of pesticides, and in winter, the survey is generally conducted 20d-~30d after the application of pesticides). The third survey: It is conducted when the weed control effect is the best. The fourth survey: It is conducted after the new weeds in the pesticide-treated area have sprouted or before the vegetables are harvested (lasting effect survey). 4.3.3 Calculation method of control effect
Before sowing and before seedling after sowing and the last adjustment of post-seedling application, the fresh weight or plant height of each buried control effect is calculated according to formula (1), and the control effect of post-seedling application except the last investigation is calculated according to formula (2): 3
NY/T 1464. 35--2010
Where:
E-control effect.%
C-number of live weeds in the blank control area (or fresh);
T-number of remaining weeds in the treatment area (or fresh weight); X,-number of weeds in the treatment area before application; X...number of remaining weeds in the treatment area after application. The calculation result shall be rounded to two decimal places.
4.4 Crop Survey
4.4.1 Survey Method
E=X=×i×100
..........
...-+-..(2)
Observe whether the pesticide has any effect on the growth of the crops. If there is any, record the type and extent of the damage. The following requirements can be followed: a) Record the time and symptoms of the damage in detail (such as growth inhibition, chlorosis, sticky spots, deformity, etc.). At the same time, observe the interaction between the damage and the adverse factors (cultivation methods, inversion, disease invasion, long-term high temperature or freezing damage, etc.). b) If the damage can be counted or measured, measure the absolute value, such as the rate of damaged plants or plant height. d) In other cases, the extent and rate of damage can be estimated by the following two methods. 1) Compare the pesticide-treated area with the control area to evaluate the pesticide damage rate. 2) According to the pesticide damage grading method, grade and score the pesticide damage in each plot: Level 1: normal growth, no symptoms; Level 2: no obvious pesticide damage, the symptom rate is less than 10%, and the yield is not affected; Level 3: slight pesticide damage, which can be recovered, and the yield is not affected; Level 4: moderate pesticide damage, difficult to recover, resulting in a significant reduction in yield; Level 5: severe pesticide damage, which cannot be recovered, resulting in a serious reduction in yield or no yield. 4.4.2 Survey time and frequency
Please check the time and frequency according to the pesticide application. The actual survey frequency is as follows: a) Apply pesticides (soil treatment agents) before sowing or before seedlings emerge after sowing. The first survey: Conducted after all the seedlings in the control area emerge. Observe whether there is delayed emergence of seedlings in the treatment area, use the absolute number adjustment method to investigate the seedling situation, and count the number of seedlings. The second investigation: Conduct it during the vigorous growth period of the control vegetables or the period when the test agent is most effective, and observe for any signs of drug damage. If there is drug damage, carefully record the symptoms of drug damage, the damage situation and the time required for the vegetables to return to normal growth. At the same time, use the absolute number adjustment method to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. The third investigation: Conduct sample measurement before the vegetable is harvested. b) Apply the drug after emergence (purpurinol treatment agent). Observe for drug damage at 3d, 5d, 7a, 10d and 15d after application. If there is drug damage, the degree of drug damage should be recorded in detail, and the time required for the vegetables to return to normal growth should be observed and recorded. If there is drug damage, the absolute value method should be used when the damage symptoms are most obvious to measure the growth indicators such as plant height, leaf age and fresh weight of the vegetables. Before harvesting vegetables, take samples to measure yield.
4.5 Yield and quality survey
The yield measurement area of each test plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches. The yield of the plot is the sum of the yield of each batch. Yield is usually expressed in kg/hm2. If required, the quality of vegetables should also be assessed according to relevant standards. 6 Side effect observation
Record the impact on non-target organisms.
5 Results
NY/T1464.35—2010
The results obtained in the test were analyzed using biological statistical methods, and a test report was written in a formal format, and the test results were analyzed and evaluated. The original data should be kept for examination and verification. If the pesticide shows signs of long-lasting effectiveness in the test, subsequent crop safety tests should be carried out.The third survey: Take samples to measure yield before harvesting vegetables. b) Apply pesticide (purpurogenous pyridine treatment agent) after emergence. Observe whether there is any pesticide damage at 3d, 5d, 7a, 10d, and 15d after application. If there is any pesticide damage, the symptoms and degree of the pesticide should be recorded in detail, and the time required for the vegetables to resume normal growth should be observed and recorded. If there is any pesticide damage, the absolute value method should be adjusted when the symptoms are most obvious, and the growth indicators such as plant height, leaf age and fresh weight of the vegetables should be measured. Samples should be taken to measure yield before harvesting vegetables.
4.5 Yield and quality survey
The yield measurement area of each experimental plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches, and the yield of 4 plots is the sum of the yields of each batch. The yield is usually expressed in kg/hm2. If required, the quality of the vegetable dye should also be assessed according to the relevant standards. 6 Side effects observation
Record the impact on non-target organisms,
5 Results
NY/T1464.35—2010
The results obtained from the test should be analyzed using biological statistical methods, and a test report should be written in a formal format, and the test results should be analyzed and evaluated. The original data should be kept for examination and verification. If the agent shows signs of long-lasting effectiveness in the test, a safety test for subsequent crops should be carried out. 5The third survey: Take samples to measure yield before harvesting vegetables. b) Apply pesticide (purpurogenous pyridine treatment agent) after emergence. Observe whether there is any pesticide damage at 3d, 5d, 7a, 10d, and 15d after application. If there is any pesticide damage, the symptoms and degree of the pesticide should be recorded in detail, and the time required for the vegetables to resume normal growth should be observed and recorded. If there is any pesticide damage, the absolute value method should be adjusted when the symptoms are most obvious, and the growth indicators such as plant height, leaf age and fresh weight of the vegetables should be measured. Samples should be taken to measure yield before harvesting vegetables.
4.5 Yield and quality survey
The yield measurement area of each experimental plot varies according to the cultivation type of vegetables. For densely planted and scattered vegetables, it should not be less than 1/3 of the plot area. For row-sown vegetables, the middle 2 to 4 rows of the plot should be harvested, and the length should not be less than 1/2 of the length of the plot. Varieties that are harvested in batches should be measured in batches, and the yield of 4 plots is the sum of the yields of each batch. The yield is usually expressed in kg/hm2. If required, the quality of the vegetable dye should also be assessed according to the relevant standards. 6 Side effects observation
Record the impact on non-target organisms,
5 Results
NY/T1464.35—2010
The results obtained from the test should be analyzed using biological statistical methods, and a test report should be written in a formal format, and the test results should be analyzed and evaluated. The original data should be kept for examination and verification. If the agent shows signs of long-lasting effectiveness in the test, a safety test for subsequent crops should be carried out. 5
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