HY/T 140-2011 Specification for Survey of Marine Microbenthos HY/T140-2011 Standard compression package decompression password: www.bzxz.net
This standard defines the basic requirements for survey of marine microbenthos, and establishes general provisions and methods such as sampling and fixation, separation and cultivation, centrifugation and extraction, staining and slide preparation, qualitative and quantitative analysis. This standard is applicable to survey of marine microbenthos in different habitats such as intertidal zone, shallow sea and deep sea. ||tt
||This standard is drafted in accordance with the rules given in GB/T1.1-2009.
Please note that some contents of this document may involve patents. The issuing agency of this document does not assume the responsibility for identifying these patents.
This standard is proposed by the Institute of Oceanology, Chinese Academy of Sciences.
This standard is under the jurisdiction of the National Technical Committee for Marine Standardization (SAC/TC283).
The drafting unit of this standard is: Institute of Oceanology, Chinese Academy of Sciences.
The main drafters of this standard are: Lei Yanli, Xu Kuidong.
The following documents are indispensable for the application of this document. For all dated referenced documents, only the dated version applies to this document. For all undated referenced documents, the latest version (including all amendments) applies to this document.
GB/T12763.1 Specifications for marine surveys Part 1: General
GB/T12763.6 Specifications for marine surveys Part 6: Marine biological surveys
Foreword III
Introduction IV
1 Scope1
2 Normative references1 3
Terms and definitions1
4 Basic requirements1
4.1 Main observations1
4.2 Auxiliary observations1
4.3 Observation sites and hierarchy design1
4.4 Documents and drawings2
4.5 Technical requirements and procedural steps for surveys2
4.6 Quality plan and quality control measures2
5 Sampling and fixation2
5.1
5.2 Sampling in the intertidal zone3 5.3
Collection of living samples3
5.4 Sampling tubes3
5.5 Sample processing3
5.6 Determination of environmental factors3
6 Survey of living micro-benthic organisms4
6.1 Overview of methods4
6.2 Equipment and materials4
6.3 Sample separation and culture4
6.4 Observation and record analysis5
7 Survey of benthic microalgae and benthic bacteria5
7.1 Overview of methods5
7.2 Fluorescent staining for laboratory operations5
7.3 Fluorescent staining for shipboard operations7
8 Survey of benthic protozoa8
8.1 Method overview8
8.2 Tools and reagents8
8.3 Percoll centrifugation separation technique8
8.4 Ludox centrifugation separation technique9
8.5 Quantitative protein silver staining technique10
9 Qualitative and quantitative analysis of microbenthic organisms13
9.1 Microscopic analysis13
9.2 Calculation of common community parameters13
9.3 Filling in reports14
9.4 Statistical analysis and drawing14
10 Content and requirements of survey results14
10.1 Original records14
10.2 Documents15
10.3 Metadata16
10.4 Illustrations17
10.5 Survey conclusions 17
10.6 Quality assessment of the results 17
Appendix A (Normative Appendix) Several main groups of microbenthic organisms Figure 18
Appendix B (Informative Appendix) Microbenthic sampling and microscopic examination record table 19
Appendix C (Normative Appendix) Several instruments and equipment for microbenthic surveys Figure 21
Appendix D (Normative Appendix) Biomass conversion coefficient table of microbenthic organisms 22
Appendix E (Normative Appendix) Several key technical flow charts for microbenthic surveys 23
Appendix F (Normative Appendix) Calculation formula for biovolume of microbenthic organisms 25
Appendix G (Normative Appendix) Recipes and preparations of various solutions 27
References 29
Figure A.1 Several main groups of microbenthic organismsFigure 18
Figure C.1 Vacuum filtration systemFigure 21
Figure C.2 Filter basketFigure 21
Figure E.1 Fluorescence staining technology flow chart of benthic microalgae and bacteriaFigure 23
Figure E.2 Percoll centrifugal separation technology flow chart23
Figure E.3 Ludox centrifugal separation technology flow chart24
Figure E.4 Quantitative protein silver staining (QPS) technology flow chart24
Figure F.1 Biovolume calculation formula of microbenthic organismsFigure 25
Table B.1 Microbenthic organism sampling record table19
Table B.2 Microscopic examination record table of microbenthic organisms20
Table D.1 Biomass conversion coefficient table of microbenthic organisms22

ICS 07. 060
Marine Industry Standard of the People's Republic of Chinawww.bzxz.net
HY/T140—2011
Specifications for the survey of marine micro-benthic organisms
microbenthos2011-05-09 Issued
State Oceanic Administration
2011-06-01 Implementation
1 Model State
Normative reference documents
Terms and definitions
Basic requirements
Main body survey
Auxiliary observation panels
Observation sites and hierarchy design
Documents and drawings
Technical requirements and process steps for survey
Quality plan and quality control measures
Sampling and fixation
Avoid sampling
Tidal zone sampling
Collection of living samples
Sample tube
Sample treatment
5.6 Determination of environmental endonucleases
6 Survey of living micro-benthic organisms
Method overview
Equipment and materials
Sample separation and culture
Observation and record analysis
Benthic microalgae and benthic algae
Method overview
7.2 Fluorescent staining using laboratory instruments
7.3 Fluorescent staining using laboratory instruments
8 Survey of benthic protozoa
8.1 Method overview
8.2 Tools and reagents
8.3 Percoll centrifugation separation technique
8. 4Ludx centrifugal separation technology
8.5Fixed egg silver staining technology..
9Qualitative and quantitative analysis of microbenthic organisms
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HY/T 140—2011
HY/1 140—2011
9.1Microscopic analysis
9.2Calculation of common community parameters
9.3Filling in reports·
9.4Statistical analysis and drawing·
10Contents and requirements of survey results
Original records
Documents·
Metadata
[0.5Survey conclusions
10.6Quality assessment of results
Appendix A (Normative Appendix) List of all major groups of microbenthic organisms A (Informative Appendix) Record form for sampling and microscopic examination of microbenthic organisms Appendix (Normative Appendix) Diagram of instruments and equipment for microbenthic organism survey Appendix D (Normative Appendix) Table of biomass conversion coefficients of microbenthic organisms Appendix (Exemplary Appendix) Flow chart of several key technologies for microbenthic organism survey Appendix F (Exemplary Appendix)
Appendix G (Normative Appendix)
References
Figure 1
Formula for calculating the biovolume of microbenthic organisms Formula and preparation of various biofilms
Several major groups of microbenthic organisms Diagram
Vacuum filtration system Diagram
Filter membrane basket Diagram
Flow chart of fluorescent staining technology for microalgae and bacteria Figure E.2 Flow chart of Troll centrifugal separation technology... Flow chart of Ludox centrifugal separation technology
Quantitative silver staining (QPS) technology Technical flow chart
Biomass calculation formula of microbenthic organisms Microbenthic organism sampling record table
Microbenthic organism microscopic examination record table
Table 1 Biomass conversion coefficient table of microbenthic organisms TTTKAONATKACA
This standard was drafted according to the rules given in (H/T1.1-2009HY/T140-2011
Please note that some contents of this document may involve patents. The issuing agency of this document does not bear the responsibility for identifying these patents. This standard was proposed by the Institute of Oceanology, Chinese Academy of Sciences. This standard is under the jurisdiction of the National Technical Committee for Marine Standardization (SAT No. 283). The drafting unit of this standard is: Institute of Oceanology, Chinese Academy of Sciences. The main drafters of this standard are: Erli, Xu Quandong. E
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HY/T 140—2011
This standard is formulated to fill the gap in GB/12763.6 or the 6th part of the marine survey specification. The lack of provisions on the investigation of micro-benthic organisms in the marine plankton survey.
In order to improve the content of marine surveys in my country, provide accurate, advanced and practical research methods for the investigation of marine micro-benthic organisms, and realize the systematic integration research of large, small and micro-benthic organisms, the "908 Special Office" of the State Oceanic Administration organized the formulation of this standard. This standard is applicable to the investigation of marine micro-benthic organisms in different habitats such as intertidal zones, shallow seas and deep seas, and plays an important role in ensuring and supporting the smooth development of the investigation work, unifying the investigation procedures, operation requirements and data processing methods. TTTKANTKACA
1 Scope
Specification for Survey of Marine Microbenthic Organisms
HY/T 140—2011
This standard defines the basic requirements for survey of marine microbenthic organisms. It establishes general provisions and methods for sampling and fixation, separation and cultivation, centrifugation and extraction, staining and slide preparation, qualitative and quantitative analysis, etc. This standard is applicable to survey of marine microbenthic organisms in different habitats such as intertidal zones, shallow seas and deep seas. 2 Normative References
The following documents are indispensable for the application of this document. For any referenced document with a date, only the version with the date is applicable to this document. For any undated referenced document, the latest version (including all amendments) shall apply to this document. GB/T12763.1 Marine Survey Specification Part 1: General GB/T12763.6 Marine Survey Specification Part 6 Marine Biological Survey 3 Terms and Definitions
The following terms and definitions apply to this document: 3.1
Microbenthalid organisms
microhcnthos
All unicellular nuclei and eukaryotic microorganisms living on the surface and bottom of sediments and retained by 2.22μm filters. Note 1: Microbenthalid organisms mainly include benthic microorganisms (benthicchacleria), cyanobacteria (cyanobacteria), benthic microalgae (benthicmicroalgae) and benthic protozoa
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