NY/T 1156.12-2008 Guidelines for Indoor Bioassay Tests for Pesticides Fungicides Part 12: Pot Method for Control of Late Blight NY/T1156.12-2008 Standard Download Decompression Password: www.bzxz.net

ICS 65.100
Agricultural Industry Standard of the People's Republic of China
NY/T1156.12—2008
Guideline for laboratory bioassay of pesticidesPart 12:Potted plant test for fungicide control late blight [Phytophthorainfestans (Mont.) de Baryl on potato and tomato2008-05~16 Released
2008-07-01 Implementation
The Ministry of Agriculture of the People's Republic of China released
"Standards for Indoor Bioassay Tests of Agricultural Fungicides" is a series of standards: Part 1: Slide method for inhibiting the germination of pathogenic fungi; Part 2: Blood method for inhibiting the growth of pathogenic fungi; Part 3: Blood slide method for inhibiting cucumber frostbite; Part 4: Pot method for controlling wheat powdery mildew; Part 5: Broad bean leaf method for inhibiting rice sheath blight; Part 6: Determination of the combined effect of mixtures; Part 7: Test method for controlling cucumber frost screen;|| tt||- Part 8: Pot method for control of rice blast;
- Part 9: Leaf method for control of gray dew fungus; Part 10: Pot method for control of gray chaff: Part 11: Pot method for control of cucurbit powdery mildew;- Part 12: Pot method for control of late blight;- Part 13: Plate method for control of late blight;: Part 14: Golden pot method for control of cucurbit charcoal;
- Part 15: Control of leaf rust of cucurbits; Part 16: Pot method for control of bacterial growth:
Deep turbidity method:
This part is part 12 of the "Guidelines for Indoor Bioassay of Pesticides for Fungicides". This part is proposed and managed by the Ministry of Agriculture of the People's Republic of China. Drafting unit of this part: Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this part: Liu Xue, Zhu Chunyu, Xu Wenping, Wu Xinping, Shuai Shankui, Zhang Wei, Yang Jun NY/T 1156. 12—2008
1 Scope
Guidelines for indoor bioassay tests of pesticides Fungicides Part 12: Tests for the control of late blight: Pot method This part specifies the pot method test method for the control of late blight by fungicides. NY/T 1156. 12—2008
This part is applicable to the indoor bioassay test of fungicides for the control of late blight of tomatoes and potatoes for registered pesticides. 2 Instruments and equipment
General laboratory routine instruments and equipment.
2.1 Electronic balance (sensitivity 0.1mg).
2.2 Spraying equipment.
2.3 Artificial climate chamber.
2.4 Pipette or pipette, etc.
3 Reagents and Materials
Methods All reagents used, if the specifications are not specified, are of analytical grade; water is steamed stuffing water, 3.1 Biological Test Materials
The test pathogen is a wild sensitive pathogenic fungus Phytohthorainfestan (Mont.) deBary strain. Record the source of the strain. The test crops are mushroom or potato varieties, which are potted and cultured to the stage of 2 to 4 true leaves and numbered for use. , 3.2 Test Agents
Technical Agents (or Mother Drugs).
3.3 Control Agents
Use the technical agents (or mother drugs) commonly used in the production of registered products, and their chemical structure type or mode of action should be the same or similar to that of the test agents.
4 Test Procedures
4. 1. Preparation of zoospore suspension
Cultivate the pathogenic bacteria for testing on a suitable culture medium, or culture the diseased tissue in a moisturizing manner. After the sporangium is produced, wash the sporangium with sterile water, filter it with double-layer gauze, and prepare a suspension of spore mushrooms. Place it at a low temperature of 4℃ and treat it in the dark for 0.5h-3h to release the zoospores. Adjust the spore concentration to 1×105 spores/mL for standby use, or directly prepare a 1×195 spore/mL suspension as an inoculum. 4.2 Preparation of drugs
Water-soluble drugs are directly dissolved and diluted with distilled water. Other drugs are dissolved in suitable solvents (methanol, propylene glycol, dimethylformamide or dimethoate, etc.), and diluted with 0.1% Tween 80 or other aqueous surfactant solutions. According to the activity of the drug, set 5 to 7 series of mass concentrations, and the final content of organic solvents generally does not exceed 0.5% to 1%. The preparation can be directly diluted with water, 4. 3 Chemical treatment
Apply the liquid medicine evenly to the leaves until they are completely wetted, and use them after the liquid medicine has dried. Treat 3 pots each time, repeat 4 times, and set up a treatment containing only solvents and surfactants but no active ingredients as a blank control. 4.4 Inoculation and culture
NY/T 1156.12—2008
Spray inoculation with sporangium or zoospore suspension. Protective tests are generally inoculated about 24 hours after chemical treatment: therapeutic tests are generally inoculated 24 hours before chemical treatment. The inoculated piece is kept in a continuous light/dark alternation of 12 hours each day (light intensity 5000Lux~20000) at a temperature of 18℃~20℃, and there is a water film on the surface within 2 hours after inoculation. Thereafter, the relative filtration rate is above 90%. Under the above conditions, the plants were raised in towers for 7 l
4.5 Investigation
When the diseased leaf rate of the blank control reached more than 50%, the disease situation of each treatment was investigated in a graded manner. At least 30 leaves were investigated for each treatment. The grading method was:
Grade 0: disease-free;
Grade 1: only a few small lesions on the leaves, and the lesions accounted for less than 10% of the leaf area; Grade 3: the lesions on the leaves accounted for 10% to 25% of the leaf area; Grade 5: the diseased spots on the leaves accounted for 25% to 50% of the leaf area %; 7: spots on leaves occupy more than 50% of the leaf area; 9: all leaves are affected by wilt.
5 Statistical analysis
5.1 Calculation method
According to the survey data, calculate the disease index and control effect of each treatment. 5.1.1 Disease index
Calculate according to formula (1), and keep two decimal places: X
Where:
X—…disease index; www.bzxz.net
N.—levels Number of diseased leaves;
i-value of the diseased leaf group;
-total number of leaves investigated.
5.1.2 Control effect
Z(N:i)
Calculate according to formula (2), and the result is rounded to two decimal places: X100
Where:
Control effect. The unit is white fraction (%):
CK-blank control disease index;
PT-drug treatment disease index.
5.2 Statistical analysis
Use standard statistical software such as TDPS (data processing system), SAS (statistical analysis system) or SI\SS (statistical program for social sciences) to analyze the logarithmic value of drug concentration and the value of control efficiency, calculate the EC, EC% and other values ​​of each drug and their 95% confidence limits, and conduct a significant difference analysis between each drug treatment.
Results and Report Writing
Analyze and evaluate the statistical results and write a formal test report. 2
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