This standard specifies the determination method of furazolidone in livestock and poultry meat and aquatic products. This standard is applicable to the inspection of furazolidone in livestock and poultry meat and aquatic products. SB/T 10387-2004 Determination of furazolidone in livestock and poultry meat and aquatic products SB/T10387-2004 Standard download decompression password: www.bzxz.net

1.s 67.120
Preparation No.: 14168-2CC4
Domestic Trade Industry Standard of the People's Republic of China SL/10387--2004
Determination of furazolidoncin in meat, poultry and aquatic products
Published on August 4, 2004
Ministry of Commerce of the People's Republic of China
Implementation on September 1, 2004
This standard is issued by the Ministry of Commerce of the People's Republic of China. Preface
SB/T10387—20G4
This standard was drafted by the Ministry of Commerce and the Ministry of Industry and Information Technology. The Ministry of Industry and Information Technology Supervision Center was responsible for the drafting. The Ministry of Industry and Information Technology Supervision Center was responsible for the drafting. The Ministry of Industry and Information Technology Supervision Center was responsible for the drafting. 1 Scope
Determination of azoles in meat and aquatic products of livestock and poultry This standard briefly describes the determination method of azoles in meat and aquatic products. This standard is applicable to the detection of azoles in meat and aquatic products. 2 Principle
SB/T10387—2004
The sample contains 2,0-dihydro-1,1-dioxane. After purification by anhydrous sodium column, the sample is filtered through a 0.5 microfiltration membrane and analyzed by HPLC.
3 Reagents and Standards
The following reagents are analytically pure unless otherwise specified. Water is deionized water 3.1 Ethylene glycol: high-purity for use as solvent,
3.2 Ethylene glycol: chromatographic stability
3.3 Ethylene dehydrate: 2:1 water (80+2c.V/V), 3.4:1 hexane.
3.5 Methanol, pure.
3.6 Anhydrous sodium sulfate, calcined at 5CT for about 4h, put into a fine tube for use, 3.7 Anhydrous sodium sulfate 6cm×1.%cm, filled with 5enl high-pressure anhydrous sodium sulfide (if no suitable end is available, a 1 ml syringe can be used instead),
3. B-1-oxane,
3.9 Phosphoric acid.
3.10 Azoles, standard purity 99.7%.
3.11 Standard stock of oxadiazole: Collect 10.0 μl of oxadiazole. Use ethyl amine aqueous solution (3.3) to decompose and dilute to 50 μl. Brown volumetric oxygen solution, store in 1 μl equivalent to 23 μl. 3.12 Standard stock of oxadiazole: Accurately take 2.0 μl of standard stock of oxadiazole into a 50 mL volumetric flask, add to a certain concentration, and obtain a solution of 8 μg/μl. After heating, accurately pipette 0.05, 0.10, 0.20, 0.50, 1, 5, 2. 0mL, 10.CmL. respectively. Add water rate scale to the core package volumetric bottle, add water rate scale, add hook, and get a standard series of liquids containing 0.4, 0.16, 0.4C, 0.0, R0, 1.601r2 per drop.
4 Apparatus and equipment
4.1 High performance liquid chromatograph: with UV detector. 4.2 Acoustic washer.
4.3 Vortex moistening spoon 1 coupon.
4.4 Business machine.
4.5 English transfer instrument. bzxz.net
4.6 Microplate injector, 35L.
5 Determination steps
5. 1 According to the sample for purification, 10 g of the crushed product (accurate to 0.01) was weighed into 100 mL of a sieve, and 125 mL of dichloromethane was added. The extract was filtered through anhydrous sodium sulfate and placed in a 3 mL evaporating bottle. 2 mL of deuterated dihydrogen 2-oxo-1-oxane was added. The mixture was filtered through a sodium thiosulfate column and placed in the same evaporating bottle. 15 mL of dihydrogen 2-oxo-1 ... . Pour the sodium pyraldehyde into the filter. Pour the solution into the filter and evaporate to dryness on a rotary evaporator (water temperature is 30: -3]. Add 1 ml of silica gel and 1 ml of n-hexane and mix on a vortex mixer for 2 min. Transfer to a 5L centrifuge for 2 min (centrifuge for 10 min). Transfer to a centrifuge tube and add 1 ml of n-hexane. Add 2 ml of charcoal to the centrifuge tube. Centrifuge for 2 min. Remove the upper layer with a pipette. layer, the lower layer of sake is filtered through 3.4> pores, and the more concentrated it is for HIL analysis
5.2 Chromatographic determination
5.2.1 Liquid chromatography reference conditions
=Chromatographic housing: Hypers]Ulxs2Cte, 250mmX4.6mm, particle size 5rm: system: 7-water (40:60), 100CmL draw 1,mI do; flow reading, 1.CmLmin
d: detection wavelength: 36:m|| tt||Let it warm up.
5.2.2 Preparation of Furazolidone Standard Addition Curve
According to the above chromatographic conditions, separate the standard working solution at each point, select 20L of each standard solution, and measure its peak. Then use the peak surface limit of the standard narrow gradient as the calibration curve, and calculate the correlation coefficient of the return curve. 5.2.3 Sample Sub-determination
Under the above chromatographic conditions, accurately take 2) L of test sample droplets and perform HP1.℃ analysis. 6 Conclusion
6. 1 Calculation
The rate of each point on the standard loop is compared with the corresponding value, and then the formula (1) is used to calculate the content of the test sample.
X--XVx10
The content of ketone in the sample, unit is grams per kilogram (m/k): The volume of the test solution, which is equivalent to the value of the standard curve, unit is grams per kilogram (m/k); - The volume of the test solution, unit is mL: The unit mass, unit is grams (g) 6.2 Detection limit
The detection limit of this method is 3.01g/m. When the value is 10, the minimum detection amount is 1.2/k. The allowable difference
The difference between the results of simultaneous or consecutive determinations shall not exceed 2.
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