This standard specifies the determination of phytic acid content in plant foods by ion exchange separation and spectrophotometry. This standard applies to cereals, beans, nuts and tubers. GB/T 5009.153-2003 Determination of phytic acid in plant foods GB/T5009.153-2003 Standard download decompression password: www.bzxz.net

TCS 67.040
National Standard of the People's Republic of China
CB/T 5009.153—2003
GB/17406—1998
Determination of phytic acid in vegetable foods
Determination of phytic acid in vegetable foods2003-08-11Promulgated
Ministry of Health of the People's Republic of China
National Chemical Engineering Administration
2004-01-01Implementation
GB/T5009.153—2003
This standard replaces GB/T740-1998 Determination of phytic acid in foods3. Compared with GB/T174351998, this standard has the following major changes: the name of the standard is changed to "Determination of plant substances in food" 3. According to the CB/T20001,4-2001 standard writing rules Part 4: Chemical analysis methods, the structure of the original standard has been revised.
This standard is issued and registered by the Ministry of Health of the People's Republic of China, and the drafting unit is: China Academy of Food and Fermentation Medicine and Food Industry Research Institute; the drafting unit is: China Food Fermentation Industry Research Institute; the main drafters of the previous standard are: Liu Wanglie, Qu Ning, Yuan Xiao; the original standard was first issued in 1598, and this standard is the first revision. 288
1 Scope
Determination of phytic acid in plant foods
This standard specifies the determination of phytic acid content in plant foods by cation exchange separation and spectrophotometry. This standard is applicable to cereals, A, nuts and tubers. 2 Principle
GBT5009.153-2003
Use cation exchange resin to adsorb phytic acid and phytic acid salts, separate them from inorganic cations and their salts and other impurities, elute with sodium chloride solution, and react the phytic acid in the eluted solution with tri-hydroxysalicylic acid mixture to produce a color reaction. The phytic acid content is proportional to the colorimetric value. Use a spectrophotometer to measure the light intensity at 500 μm from the sample and calculate the phytic acid content in the sample. 3 Reagents
3.130 g/T. Sodium hydroxide solution.
3.2t).mal/L sodium chloride extraction solution.
3.30.05 ol/1. Sodium chloride filtration solution, 3.4EG0g/L acidification sodium-hydrochloric acid extraction solution: weigh 50g anhydrous sodium sulfate and dissolve in 1.2% salicylic acid solution, and dilute to 50>T. 3.5 ferric chloride-basic salicylic acid extraction solution: weigh 1.58 ferric chloride and 15% basic salicylic acid, add water to dissolve and dilute to 500mL. Dilute 10 times with water before use.
3.6 standard alcohol, take 1.7347 sodium phytate standard (purity 28%), accurate to 0.00018, add water to dissolve and dilute to 10mL. Before use, take 5 mL of water and dilute to 500 mL, which is 0.1 mg/mL3.7 to ion exchange resin: AG1-X4 (200-200 days). 4 Instruments and equipment
4.1 Spectrophotometer.
4.2 Ion exchange base: .scmx15m,
5 Preparation of test solution
5.1 Extraction: Weigh 0.5g or 2.0g of the test group that has been crushed by explosion (about 3mg of phytic acid), extract to 0.01, put it in a triangle, add 50mL of hydrochloric acid (3.4) 5)m. Vibrate for 21 seconds, filter, and collect the test solution for later use. 5.2 Separation: 0.5 g of ion resin (3.7) was loaded into the exchange holder (1.2), and the exchange was removed by sodium hydroxide (3.2). Then the exchange column was washed with water until there was no ion. 5 ml~10 ml of the collected solution (5.1) was taken, and 1 ml of the calcium hydroxide solution (3.1) was added to make the total volume 5 ml. After cutting, it was poured into the exchange holder, and then 15 IL of water and sodium chloride solution (3.3) were used to wash the exchange column at a rate of 1 ml/min. The subsequent washing was discarded. Finally, the exchange column was eluted with sodium hydroxide (3.2), 25 ml of the sample was collected and the volume was adjusted to the scale.
No. Analysis step
6.1 T. The action line was prepared, and phytic acid solution (3.6) was accurately drawn into 0.5 ml, 1.2.9.3.g, 4.6.5.0 mL in six! 10ml colorimetric solution is supplemented with water to 5mT. Add to the reaction chain (3.5)m, and rotate at 3000r/min for 1cm. After standing for 10min-120min, add the upper liquid to 1cm colorimetric tube, measure the absorbance at 500nm, and use the absorbance as the vertical standard and the content as the coordinate: draw a line (see Figure 1) or calculate the regression equation. 289
(B/T5009.153—2003
6.2 Test reduction determination, take 5mL of the visual desorption solution (5.2), (about 0.3mg of phytic acid) in a 1mL colorimetric tube. Add 4mL of the reaction solution (3.5), unhook, and the remaining steps are the same as 6.1. Measure the intensity value, and find out the maximum phytic acid content in the test on the working curve or calculate the equivalent equation.
Calculate the phytic acid content in the test sample according to formula (1).
Test:
Phytic acid standard curve
Phytic acid content in the test control, the unit is gram per gram (m g/g）)——the content of the test solution, in milligrams (mg): the mass of the test column, in grams (gbzxZ.net
V-the volume after the determination, in milliliters (ml); V, the volume after separation, in milliliters (mL); V, the volume after the separation, in milliliters (nI); when the test solution is measured, the volume obtained by separation, in milliliter (nI). The absolute difference between two independent determination results obtained under the repeatability condition shall not exceed 5% of the arithmetic mean. 296
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